BG62892B1 - Вектор и методи за въвеждане поне на два гена врастение - Google Patents
Вектор и методи за въвеждане поне на два гена врастение Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
Landscapes
- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
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- Organic Chemistry (AREA)
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- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31139994 | 1994-11-09 | ||
| JP17012395 | 1995-05-31 | ||
| JP29325495 | 1995-10-04 | ||
| JP31343295A JP3256952B2 (ja) | 1994-11-09 | 1995-10-25 | 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法 |
| PCT/JP1995/002283 WO1996015252A2 (en) | 1994-11-09 | 1995-11-08 | Vector and methods for introducing at least two genes into a plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BG101524A BG101524A (en) | 1998-01-30 |
| BG62892B1 true BG62892B1 (bg) | 2000-10-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| BG101524A BG62892B1 (bg) | 1994-11-09 | 1997-05-29 | Вектор и методи за въвеждане поне на два гена врастение |
Country Status (25)
| Country | Link |
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| US (1) | US5965791A (es) |
| EP (2) | EP1602729A3 (es) |
| JP (1) | JP3256952B2 (es) |
| KR (1) | KR100343759B1 (es) |
| CN (1) | CN1073624C (es) |
| AP (1) | AP9700980A0 (es) |
| AR (1) | AR000479A1 (es) |
| AT (1) | ATE307211T1 (es) |
| AU (1) | AU703485B2 (es) |
| BG (1) | BG62892B1 (es) |
| BR (1) | BR9509715B1 (es) |
| CA (1) | CA2162449C (es) |
| CZ (1) | CZ138897A3 (es) |
| DE (1) | DE69534525D1 (es) |
| DK (1) | DK0716147T3 (es) |
| ES (1) | ES2248795T3 (es) |
| FI (1) | FI971961L (es) |
| HU (1) | HUT77074A (es) |
| NO (1) | NO972108L (es) |
| NZ (1) | NZ295256A (es) |
| OA (1) | OA10607A (es) |
| PL (1) | PL184707B1 (es) |
| SK (1) | SK56997A3 (es) |
| UA (1) | UA40661C2 (es) |
| WO (1) | WO1996015252A2 (es) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN903196A0 (en) * | 1996-03-29 | 1996-04-26 | Australian National University, The | Single-step excision means |
| ATE355384T1 (de) * | 1996-05-09 | 2006-03-15 | Jujo Paper Co Ltd | Vektor zum gentransfer in pflanzen, der eine wahlweise deletion des markergens erlaubt |
| US6204434B1 (en) | 1996-09-11 | 2001-03-20 | Genesis Research & Development Corporation Limited | Materials and methods for the modification of plant lignin content |
| US5850020A (en) * | 1996-09-11 | 1998-12-15 | Genesis Research & Development Corporation, Ltd. | Materials and method for the modification of plant lignin content |
| US6410718B1 (en) | 1996-09-11 | 2002-06-25 | Genesis Research & Development Corporation Ltd. | Materials and methods for the modification of plant lignin content |
| FR2759857B1 (fr) * | 1997-02-27 | 1999-04-30 | Biocem | Nouvelles utilisations de la sterilite male chez les plantes |
| US6632980B1 (en) * | 1997-10-24 | 2003-10-14 | E. I. Du Pont De Nemours And Company | Binary viral expression system in plants |
| JP2000083680A (ja) * | 1998-07-16 | 2000-03-28 | Nippon Paper Industries Co Ltd | 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ― |
| FR2784393B1 (fr) * | 1998-10-09 | 2002-02-22 | Biogemma Fr | Procede d'obtention de plantes transgeniques exprimant une proteine a activite productrice de peroxyde d'hydrogene par transformation par agrobacterium rhizogenes |
| ID30318A (id) | 1998-10-09 | 2001-11-22 | Genesis Res & Dev Corp Ltd | Bahan-bahan dan metoda untuk modifikasi kandungan lignin tanaman |
| CA2292798A1 (en) | 1999-01-06 | 2000-07-06 | Nippon Paper Industries Co., Ltd. | Novel vector for introducing a gene into a plant using a selectable marker |
| DE60037941T9 (de) | 1999-05-17 | 2009-05-28 | Icon Genetics, Inc. | SCHNELLES, VON DER VARIETäT UNABHäNGIGES PFLANZENTRANSFORMATIONSVERFAHREN |
| US6231561B1 (en) | 1999-09-20 | 2001-05-15 | Appriva Medical, Inc. | Method and apparatus for closing a body lumen |
| US6723896B1 (en) * | 1999-11-12 | 2004-04-20 | The Rockefeller University | Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants |
| CA2399469C (en) * | 2000-02-08 | 2010-12-14 | Sakata Seed Corporation | Novel methods and constructs for plant transformation |
| US7112721B2 (en) | 2000-02-08 | 2006-09-26 | Sakata Seed Corporation | Methods and constructs for plant transformation |
| US6750379B2 (en) | 2000-03-09 | 2004-06-15 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
| US6512165B1 (en) * | 2000-07-10 | 2003-01-28 | Pioneer Hi-Bred International, Inc. | Methods for enhancing plant transformation frequencies |
| US7560622B2 (en) * | 2000-10-06 | 2009-07-14 | Pioneer Hi-Bred International, Inc. | Methods and compositions relating to the generation of partially transgenic organisms |
| US6927322B2 (en) | 2001-03-30 | 2005-08-09 | Cornell Research Foundation, Inc. | Cabbage proteinase inhibitor gene confers resistance against plant pests |
| EP1264891A1 (en) * | 2001-05-31 | 2002-12-11 | Plant Research International B.V. | Modification of plant genomes by inducible site-specific recombination of transgenes |
| JP3985474B2 (ja) | 2001-07-24 | 2007-10-03 | 日本製紙株式会社 | 遺伝子導入効率を向上させたユーカリ属の植物への遺伝子導入方法 |
| EP1279737A1 (en) | 2001-07-27 | 2003-01-29 | Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. | Transformation method for obtaining marker-free plants |
| JPWO2003040363A1 (ja) * | 2001-11-08 | 2005-03-03 | 独立行政法人科学技術振興機構 | イネのトランスポゾン遺伝子 |
| WO2003069980A2 (en) * | 2002-02-20 | 2003-08-28 | J.R. Simplot Company | Precise breeding |
| US7534934B2 (en) | 2002-02-20 | 2009-05-19 | J.R. Simplot Company | Precise breeding |
| AU2003250115B2 (en) | 2002-07-26 | 2009-03-12 | Basf Plant Science Gmbh | Inversion of the negative-selective effect of negative marker proteins using selection methods |
| JP4102921B2 (ja) * | 2002-07-26 | 2008-06-18 | 日本製紙株式会社 | オーキシン前駆体を利用した遺伝子組換え植物の効率的作成方法 |
| WO2004076625A2 (en) * | 2003-02-21 | 2004-09-10 | Vector Tobacco Ltd. | Method of identifying plant cells transformed with a gene |
| WO2004087910A1 (ja) | 2003-03-28 | 2004-10-14 | National Institute Of Agrobiological Sciences | 組換えタンパク質が高生産された植物貯蔵器官の生産方法及び新規組換えタンパク質 |
| US7575917B2 (en) * | 2003-04-09 | 2009-08-18 | Monsanto Technology Llc | DNA constructs and methods to enhance the production of commercially viable transgenic plants |
| EP1699926B1 (en) | 2003-12-10 | 2014-06-18 | Monsanto Technology, LLC | Stress tolerant plants and methods thereof |
| CA2653231C (en) | 2006-05-12 | 2016-04-12 | Monsanto Technology Llc | Methods and compositions for obtaining marker-free transgenic plants |
| EP1949785A1 (en) | 2007-01-26 | 2008-07-30 | Coöperatie AVEBE U.A. | Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation |
| EP3023499A1 (en) | 2008-07-16 | 2016-05-25 | Monsanto Technology LLC | Methods and vectors for producing transgenic plants |
| US7868688B2 (en) * | 2008-12-30 | 2011-01-11 | Cosmic Circuits Private Limited | Leakage independent very low bandwith current filter |
| JP7282382B2 (ja) * | 2017-11-27 | 2023-05-29 | 国立研究開発法人理化学研究所 | ゲノム編集植物の生産方法 |
| CN108753813B (zh) * | 2018-06-08 | 2021-08-24 | 中国水稻研究所 | 获得无标记转基因植物的方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3810286A1 (de) * | 1988-03-25 | 1989-10-12 | Max Planck Gesellschaft | Transgene pflanze mit modifizierter physiologie, morphologie und modifiziertem hormonmetabolismus, gewebekulturen dieser pflanze und verfahren zu ihrer herstellung |
| US5512482A (en) | 1990-04-26 | 1996-04-30 | Calgene, Inc. | Plant thioesterases |
| US5225341A (en) * | 1990-07-19 | 1993-07-06 | The Regents Of The University Of California | Biologically safe plant transformation system using a ds transposon |
| GB9024728D0 (en) | 1990-11-14 | 1991-01-02 | Ici Plc | Herbicide resistant plants |
| WO1992020802A2 (en) | 1991-05-22 | 1992-11-26 | Mycogen Corporation | Novel bacillus thuringiensis isolates active against hymenopteran pests and genes encoding hymenopteran-active toxins |
| WO1993001283A1 (en) | 1991-07-08 | 1993-01-21 | The United States Of America As Represented By The Secretary Of Agriculture | Selection-gene-free transgenic plants |
| EP0591530A4 (en) | 1992-03-24 | 1995-05-03 | Rice Breeding Research Lab | METHOD FOR REDUCING STORED PROTEINS IN SEEDS AND METHOD FOR TRANSFORMING PLANTS. |
| DK0604662T3 (da) | 1992-07-07 | 2008-10-20 | Japan Tobacco Inc | Fremgangsmåde til transformering af monocotyledon |
| US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
| JPH06276872A (ja) | 1993-03-31 | 1994-10-04 | Mitsubishi Corp | マーカー遺伝子の除去方法 |
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- 1995-11-08 UA UA97062658A patent/UA40661C2/uk unknown
- 1995-11-08 CZ CZ971388A patent/CZ138897A3/cs unknown
- 1995-11-08 SK SK569-97A patent/SK56997A3/sk unknown
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