BG104161A - Recombinant expression of insulin c-peptide - Google Patents

Recombinant expression of insulin c-peptide

Info

Publication number: BG104161A
Authority: BG; Bulgaria
Prior art keywords: insulin; peptide; recombinant expression; polypeptide; multimer
Prior art date: 1997-08-07

Application number

BG104161A

Other languages

Bulgarian (bg)

English (en)

Inventor

Stefan Stahl

Mathias Uhlen

Per-Ake Nygren

Per Jonasson

Original Assignee

Creative Peptides Sweden Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-08-07

Filing date

2000-02-17

Publication date

2000-12-29

2000-02-17 Application filed by Creative Peptides Sweden Ab filed Critical Creative Peptides Sweden Ab

2000-12-29 Publication of BG104161A publication Critical patent/BG104161A/xx

Links

VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 title abstract 4
238000003259 recombinant expression Methods 0.000 title 1
238000000034 method Methods 0.000 abstract 3
229920001184 polypeptide Polymers 0.000 abstract 3
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
102000004196 processed proteins & peptides Human genes 0.000 abstract 2
108090000765 processed proteins & peptides Proteins 0.000 abstract 2
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
239000002253 acid Substances 0.000 abstract 1
230000015556 catabolic process Effects 0.000 abstract 1
238000006731 degradation reaction Methods 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Diabetes (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Toxicology (AREA)
Physics & Mathematics (AREA)
Chemical Kinetics & Catalysis (AREA)
Obesity (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
General Chemical & Material Sciences (AREA)
Hematology (AREA)
Emergency Medicine (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

BG104161A 1997-08-07 2000-02-17 Recombinant expression of insulin c-peptide BG104161A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB9716790.2A GB9716790D0 (en)	1997-08-07	1997-08-07	Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
PCT/GB1998/002382 WO1999007735A2 (en)	1997-08-07	1998-08-07	Recombinant expression of insulin c-peptide

Publications (1)

Publication Number	Publication Date
BG104161A true BG104161A (en)	2000-12-29

Family

ID=10817184

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
BG104161A BG104161A (en)	1997-08-07	2000-02-17	Recombinant expression of insulin c-peptide

Country Status (21)

Country	Link
US (1)	US6558924B1 (pt)
EP (1)	EP1001980A1 (pt)
JP (1)	JP2001512745A (pt)
KR (1)	KR20010022590A (pt)
CN (1)	CN1268141A (pt)
AU (1)	AU750685B2 (pt)
BG (1)	BG104161A (pt)
BR (1)	BR9811850A (pt)
CA (1)	CA2297382A1 (pt)
EA (1)	EA200000202A1 (pt)
EE (1)	EE200000072A (pt)
GB (1)	GB9716790D0 (pt)
HU (1)	HUP0004626A1 (pt)
IL (1)	IL134372A0 (pt)
IS (1)	IS5368A (pt)
NO (1)	NO20000575L (pt)
NZ (1)	NZ502450A (pt)
PL (1)	PL339085A1 (pt)
SK (1)	SK1532000A3 (pt)
TR (1)	TR200000288T2 (pt)
WO (1)	WO1999007735A2 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20040005669A1 (en) *	1997-08-07	2004-01-08	Stefan Stahl	Recombinant expression of insulin C-peptide
FR2810674A1 (fr) *	2000-06-22	2001-12-28	Pf Medicament	Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
FR2810675B1 (fr) *	2000-06-22	2002-09-27	Pf Medicament	Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes
JP4656814B2 (ja) *	2000-12-20	2011-03-23	エフ．ホフマン−ラロシュアーゲー	エリスロポエチンコンジュゲート
EP1572908A4 (en) *	2002-02-14	2008-10-22	William J Rutter	CHIMERIC MOLECULES FOR ADMINISTERING CLEAVAGE TO A TREATED HOST
CA2504287A1 (en)	2002-11-26	2004-06-10	Nobex Corporation	Natriuretic compounds, conjugates, and uses thereof
US20050026826A1 (en) *	2003-01-17	2005-02-03	Margarethe Hoenig	Feline proinsulin, insulin and constituent peptides
US7795384B2 (en) *	2003-06-03	2010-09-14	Shanghai Centre Of Research & Development Of New Drugs	Fusion protein suitable for high efficiency expression and the production method thereof
CN102504025B (zh) *	2004-03-10	2015-10-21	克赖顿大学	雌激素受体和使用方法
MX2012013375A (es)	2010-05-17	2013-04-11	Cebix Inc	Peptido c pegilado.
WO2013075117A2 (en)	2011-11-17	2013-05-23	John Wahren	Pegylated c-peptide
KR20140148420A (ko) *	2012-03-19	2014-12-31	마들렌 파마수티컬스 피티와이 리미티드	재조합 펩티드의 생산 방법
WO2016145389A1 (en)	2015-03-12	2016-09-15	Board Of Trustees Of Michigan State University	Compositions and methods for measuring c-peptide binding and diagnosing immune-mediated diseases
CN109705221B (zh) *	2018-12-27	2021-03-09	美康生物科技股份有限公司	C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5210872B2 (pt)	1973-07-14	1977-03-26
GB2068971B (en)	1980-01-30	1983-06-08	Searle & Co	Recombinant dna techniques
CA1213537A (en)	1984-05-01	1986-11-04	Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee	Polypeptide expression method
ATE113985T1 (de)	1987-03-20	1994-11-15	Creative Biomolecules Inc	Verfahren zur reinigung von rekombinanten polypeptiden.
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
SE501169C2 (sv)	1988-02-05	1994-11-28	Aake Nygren	Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US5130236A (en)	1989-12-07	1992-07-14	Eli Lilly And Company	Process for producing des(64,65)-proinsulin
US5595887A (en)	1990-07-16	1997-01-21	Bionebraska, Inc.	Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
GB9015825D0 (en)	1990-07-18	1990-09-05	Ciba Geigy Ag	In vitro processing of fusion proteins
JPH07108232B2 (ja)	1990-10-09	1995-11-22	エム・ディ・リサーチ株式会社	ペプチド又は蛋白質の製造方法
US5953418A (en)	1995-06-14	1999-09-14	David Hall	Providing selective data broadcast receiver addressability
WO1997023639A1 (en)	1995-12-22	1997-07-03	Toray Industries, Inc.	Process for producing biologically active fused proteins

1997
- 1997-08-07 GB GBGB9716790.2A patent/GB9716790D0/en active Pending
1998
- 1998-08-07 EA EA200000202A patent/EA200000202A1/ru unknown
- 1998-08-07 EE EEP200000072A patent/EE200000072A/xx unknown
- 1998-08-07 WO PCT/GB1998/002382 patent/WO1999007735A2/en not_active Ceased
- 1998-08-07 IL IL13437298A patent/IL134372A0/xx unknown
- 1998-08-07 KR KR1020007001182A patent/KR20010022590A/ko not_active Withdrawn
- 1998-08-07 BR BR9811850-1A patent/BR9811850A/pt not_active Application Discontinuation
- 1998-08-07 US US09/485,286 patent/US6558924B1/en not_active Expired - Fee Related
- 1998-08-07 NZ NZ502450A patent/NZ502450A/xx unknown
- 1998-08-07 SK SK153-2000A patent/SK1532000A3/sk unknown
- 1998-08-07 EP EP98937689A patent/EP1001980A1/en not_active Withdrawn
- 1998-08-07 AU AU86407/98A patent/AU750685B2/en not_active Ceased
- 1998-08-07 JP JP2000506237A patent/JP2001512745A/ja active Pending
- 1998-08-07 CA CA002297382A patent/CA2297382A1/en not_active Abandoned
- 1998-08-07 HU HU0004626A patent/HUP0004626A1/hu unknown
- 1998-08-07 TR TR2000/00288T patent/TR200000288T2/xx unknown
- 1998-08-07 PL PL98339085A patent/PL339085A1/xx not_active Application Discontinuation
- 1998-08-07 CN CN98808075A patent/CN1268141A/zh active Pending
2000
- 2000-02-01 IS IS5368A patent/IS5368A/is unknown
- 2000-02-04 NO NO20000575A patent/NO20000575L/no not_active Application Discontinuation
- 2000-02-17 BG BG104161A patent/BG104161A/xx unknown

Also Published As

Publication number	Publication date
EE200000072A (et)	2000-10-16
CA2297382A1 (en)	1999-02-18
AU750685B2 (en)	2002-07-25
NO20000575D0 (no)	2000-02-04
SK1532000A3 (en)	2000-09-12
NO20000575L (no)	2000-04-04
KR20010022590A (ko)	2001-03-26
IS5368A (is)	2000-02-01
BR9811850A (pt)	2000-08-08
US6558924B1 (en)	2003-05-06
EP1001980A1 (en)	2000-05-24
GB9716790D0 (en)	1997-10-15
AU8640798A (en)	1999-03-01
HUP0004626A1 (hu)	2001-04-28
CN1268141A (zh)	2000-09-27
TR200000288T2 (tr)	2000-06-21
EA200000202A1 (ru)	2001-04-23
NZ502450A (en)	2002-10-25
WO1999007735A3 (en)	1999-04-29
WO1999007735A2 (en)	1999-02-18
PL339085A1 (en)	2000-12-04
IL134372A0 (en)	2001-04-30
JP2001512745A (ja)	2001-08-28

Publication	Publication Date	Title
BG104161A (en)	2000-12-29	Recombinant expression of insulin c-peptide
US6114148C1 (en)	2012-05-01	High level expression of proteins
US5786464C1 (en)	2012-04-24	Overexpression of mammalian and viral proteins
AR056723A2 (es)	2007-10-17	Polipeptido purificado metodo para producirlo , metodo para identificarlo y/ o aislarlo composicion farmaceutica que lo comprende, secuencia de acidos nucleicos aislada que codifica dicho polipeptido, vector que contiene a dicha secuencia celula huesped transfectada por dicho vector, sonda nucleotid
NZ504548A (en)	2003-06-30	Polypeptides and nucleic acids encoding human chordin
MD960317A (ro)	1998-05-31	Noile peptide-agoniste ale amilinei şi utilizarea lor
WO1998022574A3 (de)	1998-07-09	Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
CA2094027A1 (en)	1992-04-19	Osteogenic peptides
DE69833667D1 (de)	2006-04-27	Polynukleotid kodierend für ein polypeptid mit heparanase-aktivität und dessen expression in transduzierten zellen
DE69417252D1 (de)	1999-04-22	Verbesserte immunogene zusammensetzung gegen mensliches gastrin 17
AU6991894A (en)	1995-01-17	Prostaglandin receptor fp and dna encoding it
CA2205237A1 (en)	1996-06-13	Pesticidal composition comprising cryif chimeric and cryia(c) chimeric bacillus thuringiensis delta-endotoxin
EP0372707A3 (en)	1991-09-11	Modified proteins, modified interferons, alphas and beta, phosphorylated proteins and analogues, dna sequences, applications and uses thereof
EP1380648A3 (en)	2004-04-14	Recombinant DNAase B derived from streptococcus pyogenes
AR012499A1 (es)	2000-10-18	Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin
WO1998040498A3 (en)	1999-01-14	Immunoadhesins and methods of production and use thereof
WO1996007739A3 (en)	1996-04-18	Interleukin-1 type 3 receptors
AU3390995A (en)	1996-03-29	Novel implant and novel vector for the treatment of acquired diseases
IL147350A0 (en)	2002-08-14	Neurotrophic factor receptor
WO1998025955A3 (en)	1998-07-30	Cloned glucagon-like peptide-2 receptors
WO1994029456A3 (en)	1995-03-02	Herpes simplex virus type-2 protease
AU7324094A (en)	1995-02-13	Cell surface protein present on nk (natural killer cells) named dx1
WO2000074629A3 (fr)	2001-03-22	Composition destinee a la mise en oeuvre d'un traitement cytotoxique, notamment antitumoral ou antiviral, chez un mammifere
EP0290029A3 (en)	1990-07-18	(19-alanine) calcitonin
AU6015696A (en)	1997-01-15	Modified polypeptide, dna encoding the same, transformant, and medicinal composition containing the polypeptide