BG104154A - Индуциране на мъжка стерилност в растения чрез експресия на високи нива авидин - Google Patents

Индуциране на мъжка стерилност в растения чрез експресия на високи нива авидин Download PDF

Info

Publication number: BG104154A
Authority: BG; Bulgaria
Prior art keywords: plant; gene; promoter; nucleotide sequence; avidin
Prior art date: 1997-07-14

Application number

BG104154A

Other languages

Bulgarian (bg)

English (en)

Inventor

Marc Albertsen

John Howard

Sheila Maddock

Original Assignee

Pioneer Hi-Bred International, Inc.

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-07-14

Filing date

2000-02-11

Publication date

2000-08-31

2000-02-11 Application filed by Pioneer Hi-Bred International, Inc. filed Critical Pioneer Hi-Bred International, Inc.

2000-08-31 Publication of BG104154A publication Critical patent/BG104154A/bg

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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility

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Organic Chemistry (AREA)
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Physics & Mathematics (AREA)
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Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Pregnancy & Childbirth (AREA)
Reproductive Health (AREA)
Pharmacology & Pharmacy (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

BG104154A 1997-07-14 2000-02-11 Индуциране на мъжка стерилност в растения чрез експресия на високи нива авидин BG104154A (bg)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US08/893,049 US5962769A (en)	1995-06-07	1997-07-14	Induction of male sterility in plants by expression of high levels of avidin
PCT/US1998/008065 WO1999004023A1 (en)	1997-07-14	1998-04-22	Induction of male sterility in plants by expression of high levels of avidin

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BG104154A true BG104154A (bg)	2000-08-31

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Country Status (22)

Country	Link
US (1)	US5962769A (es)
EP (1)	EP1000164B1 (es)
JP (1)	JP2001510052A (es)
KR (1)	KR20010021940A (es)
CN (1)	CN1150330C (es)
AR (1)	AR015402A1 (es)
AT (1)	ATE310826T1 (es)
AU (1)	AU742366B2 (es)
BG (1)	BG104154A (es)
BR (1)	BR9811697A (es)
CA (1)	CA2296445A1 (es)
CR (1)	CR5812A (es)
DE (1)	DE69832489T2 (es)
ES (1)	ES2253813T3 (es)
HU (1)	HU225685B1 (es)
MX (1)	MXPA00000580A (es)
NZ (1)	NZ502688A (es)
RO (1)	RO120920B1 (es)
SK (1)	SK712000A3 (es)
TR (1)	TR200000098T2 (es)
WO (1)	WO1999004023A1 (es)
ZA (1)	ZA986087B (es)

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Publication number	Priority date	Publication date	Assignee	Title
IL125632A0 (en) *	1998-08-03	1999-04-11	Israel State	Degeneration and restoration of plant tissue
ATE476513T1 (de) *	1998-12-22	2010-08-15	Dow Agrosciences Llc	Verfahren zum limitieren des herauskreuzens und unerwünschten genflusses in nutzpflanzen
DE10015862A1 (de)	2000-03-30	2001-10-11	Bayer Ag	Polyamidzusammensetzung und Verfahren zu ihrer Herstellung
US7517975B2 (en)	2000-09-26	2009-04-14	Pioneer Hi-Bred International, Inc.	Nucleotide sequences mediating male fertility and method of using same
WO2002052924A2 (en) *	2001-01-04	2002-07-11	Yeda Research And Development Co. Ltd.	Method to maintain a genic male-sterile female parental lines for the production of hybrid wheat
US20020129399A1 (en) *	2001-03-09	2002-09-12	Marc Albertsen	Biotin-binding compounds for induction of sterility in plants
US7230168B2 (en) *	2001-12-20	2007-06-12	The Curators Of The University Of Missouri	Reversible male sterility in transgenic plants by expression of cytokinin oxidase
FR2836782B1 (fr) *	2002-03-08	2004-06-04	Biogemma Fr	Nouveau procede de production de semences hybrides de mais
US7667096B2 (en)	2003-06-03	2010-02-23	University Of Georgia Research Foundation, Inc.	Conditional sterility in plants
BRPI0411030A (pt)	2003-06-06	2006-09-19	Arborgen Llc	composições de meio, meio de pré-cultura de eucalyptus, plantas transgênicas, polpa, fibra, óleo, papel e chá da madeira e métodos de transformação de pelo menos uma célula de explante de árvore com dna estranho, de produção de árvores e plantas não quiméricas e transgênicas, de madeira, de polpa, de óleo, de papel da madeira e de seleção de célula de planta transformada
DE60329236D1 (de) *	2003-07-07	2009-10-22	Univ Delhi South Campus	Methode zur herstellung verbesserter fruchtbarkeitswiederherstellungslinien für sterile männliche transgene nutzpflanzen und eine dns-konstruktion zur benutzung in dieser methode
ES2339559T3 (es) *	2003-12-16	2010-05-21	Pioneer Hi-Bred International, Inc.	Transgenes de supresion de gen dominante y metodos de uso de los mismos.
WO2005070088A2 (en) *	2004-01-15	2005-08-04	University Of Georgia Research Foundation, Inc.	Chimeric sequences for tissue-specific gene expression in plants
US7453025B2 (en) *	2004-09-22	2008-11-18	Arborgen, Llc	Reproductive ablation constructs
US20060101536A1 (en) *	2004-11-05	2006-05-11	Arborgen, Llc	Eucalyptus urophylla transformation and regeneration
WO2009020458A1 (en)	2007-08-03	2009-02-12	Pioneer Hi-Bred International, Inc.	Msca1 nucleotide sequences impacting plant male fertility and method of using same
US8115060B2 (en) *	2008-02-22	2012-02-14	Applied Biotechnology Institute, Inc.	Means and methods of controlling plant seed germination
EP2612918A1 (en)	2012-01-06	2013-07-10	BASF Plant Science Company GmbH	In planta recombination
CN104703998B (zh)	2012-03-13	2020-08-21	先锋国际良种公司	植物中雄性育性的遗传减少
WO2013138309A1 (en)	2012-03-13	2013-09-19	Pioneer Hi-Bred International, Inc.	Genetic reduction of male fertility in plants
EP2781151A1 (en)	2013-03-18	2014-09-24	Bayer CropScience AG	Methods of separating hybrid seed from a mixture of seeds
WO2015101243A1 (zh)	2014-01-02	2015-07-09	北京思创达科技有限公司	雌性不育系的繁殖及杂交制种技术
EP4118955A1 (en)	2015-03-19	2023-01-18	Pioneer Hi-Bred International, Inc.	Compositions for accelerated trait introgression
US10934556B2 (en)	2015-04-16	2021-03-02	Council Of Scientific And Industrial Research	Reversible expression system for transgene expression in plants

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2624470B2 (ja) *	1984-10-02	1997-06-25	バイオジェンインコーポレイテッド	ストレプトアビジン様ポリペプチドの製造
US5356799A (en) *	1988-02-03	1994-10-18	Pioneer Hi-Bred International, Inc.	Antisense gene systems of pollination control for hybrid seed production
EP0342926B1 (en) *	1988-05-17	1994-09-28	Mycogen Plant Science, Inc.	Plant ubiquitin promoter system
DK0412006T3 (da) *	1989-08-04	2001-03-05	Aventis Cropscience Nv	Planter med modificerede blomster, frø eller embryoer
DE3926390A1 (de) *	1989-08-10	1991-02-14	Bayer Ag	Verwendung von lysozym genen in pflanzen zur resistenzerhoehung
US5225341A (en) *	1990-07-19	1993-07-06	The Regents Of The University Of California	Biologically safe plant transformation system using a ds transposon
WO1994000992A1 (en) *	1992-07-10	1994-01-20	Pioneer Hi-Bred International, Inc.	Avidin and homologues as larvicides against insect pests
US5583210A (en) *	1993-03-18	1996-12-10	Pioneer Hi-Bred International, Inc.	Methods and compositions for controlling plant development
US5837850A (en) *	1994-04-21	1998-11-17	Pioneer Hi-Bred International, Inc.	Regulatory element conferring tapetum specificity
US5633438A (en) *	1994-11-22	1997-05-27	Pioneer Hi-Bred International	Microspore-specific regulatory element
US5837851A (en) *	1994-12-08	1998-11-17	Pioneer Hi-Bred International, Inc.	DNA promoter 5126 and constructs useful in a reversible nuclear genetic system for male sterility in transgenic plants
RO120270B1 (ro) *	1995-06-07	2005-11-30	Pioneer Hi-Bred International Inc.	Inducerea sterilităţii masculine la plante, prin expresia la nivele ridicate a avidinei
US5767379A (en) *	1995-11-06	1998-06-16	John Howard	Commercial production of avidin in plants

1997
- 1997-07-14 US US08/893,049 patent/US5962769A/en not_active Expired - Lifetime
1998
- 1998-04-22 HU HU0002649A patent/HU225685B1/hu not_active IP Right Cessation
- 1998-04-22 RO ROA200000048A patent/RO120920B1/ro unknown
- 1998-04-22 CN CNB988089157A patent/CN1150330C/zh not_active Expired - Fee Related
- 1998-04-22 WO PCT/US1998/008065 patent/WO1999004023A1/en not_active Ceased
- 1998-04-22 TR TR2000/00098T patent/TR200000098T2/xx unknown
- 1998-04-22 NZ NZ502688A patent/NZ502688A/xx unknown
- 1998-04-22 DE DE69832489T patent/DE69832489T2/de not_active Expired - Lifetime
- 1998-04-22 BR BR9811697-5A patent/BR9811697A/pt not_active Application Discontinuation
- 1998-04-22 JP JP2000503229A patent/JP2001510052A/ja active Pending
- 1998-04-22 AU AU71460/98A patent/AU742366B2/en not_active Ceased
- 1998-04-22 AT AT98918556T patent/ATE310826T1/de not_active IP Right Cessation
- 1998-04-22 KR KR1020007000505A patent/KR20010021940A/ko not_active Ceased
- 1998-04-22 EP EP98918556A patent/EP1000164B1/en not_active Expired - Lifetime
- 1998-04-22 CA CA002296445A patent/CA2296445A1/en not_active Abandoned
- 1998-04-22 ES ES98918556T patent/ES2253813T3/es not_active Expired - Lifetime
- 1998-04-22 MX MXPA00000580A patent/MXPA00000580A/es unknown
- 1998-04-22 SK SK71-2000A patent/SK712000A3/sk unknown
- 1998-07-09 ZA ZA9806087A patent/ZA986087B/xx unknown
- 1998-07-13 AR ARP980103402A patent/AR015402A1/es not_active Application Discontinuation
- 1998-07-14 CR CR5812A patent/CR5812A/es not_active Application Discontinuation
2000
- 2000-02-11 BG BG104154A patent/BG104154A/bg unknown

Also Published As

Publication number	Publication date
EP1000164B1 (en)	2005-11-23
AR015402A1 (es)	2001-05-02
WO1999004023A1 (en)	1999-01-28
ATE310826T1 (de)	2005-12-15
KR20010021940A (ko)	2001-03-15
ZA986087B (en)	2000-05-16
EP1000164A1 (en)	2000-05-17
CN1269837A (zh)	2000-10-11
AU7146098A (en)	1999-02-10
DE69832489D1 (de)	2005-12-29
US5962769A (en)	1999-10-05
HUP0002649A2 (hu)	2000-12-28
RO120920B1 (ro)	2006-09-29
NZ502688A (en)	2002-12-20
MXPA00000580A (es)	2005-09-08
DE69832489T2 (de)	2006-07-20
HUP0002649A3 (en)	2002-01-28
TR200000098T2 (tr)	2000-11-21
JP2001510052A (ja)	2001-07-31
HU225685B1 (hu)	2007-06-28
CR5812A (es)	2001-02-15
AU742366B2 (en)	2002-01-03
BR9811697A (pt)	2002-01-02
CN1150330C (zh)	2004-05-19
SK712000A3 (en)	2000-08-14
ES2253813T3 (es)	2006-06-01
CA2296445A1 (en)	1999-01-28

Publication	Publication Date	Title
BG104154A (bg)	2000-08-31	Индуциране на мъжка стерилност в растения чрез експресия на високи нива авидин
AU708618B2 (en)	1999-08-05	Induction of male sterility in plants by expression of high levels of avidin
JP2000515020A (ja)	2000-11-14	植物の成長および収穫量を増大させる方法
HUT73336A (en)	1996-07-29	Methods and compositions for controlling plant development
HU219543B (hu)	2001-05-28	Eljárás hímsteril növények és az eljárásban használható rekombináns DNS előállítására
JP2002354954A (ja)	2002-12-10	トランスジェニック植物における雄性不稔のための可逆的核遺伝システム
JP2000510342A (ja)	2000-08-15	アポミクト種子の作成
HU225428B1 (en)	2006-11-28	Methods for altering organ mass, controlling fertility and enhancing asexual reproduction in plants
KR100505908B1 (ko)	2005-08-05	조건적 자성 불임을 사용하는 잡종 종자 생산 방법
US20090151025A1 (en)	2009-06-11	Indeterminate Gametophyte 1 (ig1), Mutations of ig1, Orthologs of ig1, and Uses Thereof
JP2002507381A (ja)	2002-03-12	核の雄性不稔性植物、雄性不稔性植物を作出する方法および稔性を回復するための方法
JPH11509417A (ja)	1999-08-24	細胞呼吸の阻害および雄性不稔植物の生成
US7056739B1 (en)	2006-06-06	Compositions and methods for modulation of plant cell division
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CZ2000182A3 (cs)	2000-07-12	Vyvolání samčí sterility u rostlin silnou expresí avidinu
MXPA97009731A (es)	1998-10-15	Induccion de esterilidad masculina en plantas mediante la expresion de altos niveles de avidina
CZ339899A3 (cs)	2000-08-16	Ztracené bednění stropů
HU221518B (hu)	2002-11-28	Hímfertilitást közvetítő nukleotidszekvenciák és eljárások azok alkalmazására