AU2001227768A1 - Improved synthesis of sulfurized oligonucleotides - Google Patents

Improved synthesis of sulfurized oligonucleotides

Info

Publication number: AU2001227768A1
Authority: AU; Australia
Prior art keywords: improved synthesis; sulfurized oligonucleotides; sulfurized; oligonucleotides; synthesis
Prior art date: 2000-01-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU2001227768A

Other languages

English (en)

Inventor

Zacharia S. Cheruvallath

Douglas L. Cole

Vasulinga T. Ravikumar

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ionis Pharmaceuticals Inc

Original Assignee

Isis Pharmaceuticals Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-01-11

Filing date

2001-01-10

Publication date

2001-07-24

2001-01-10 Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc

2001-07-24 Publication of AU2001227768A1 publication Critical patent/AU2001227768A1/en

Status Abandoned legal-status Critical Current

Links

108091034117 Oligonucleotide Proteins 0.000 title 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
230000015572 biosynthetic process Effects 0.000 title 1
238000003786 synthesis reaction Methods 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Saccharide Compounds (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

AU2001227768A 2000-01-11 2001-01-10 Improved synthesis of sulfurized oligonucleotides Abandoned AU2001227768A1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US09481486		2000-01-11
US09/481,486 US6242591B1 (en)	1997-10-15	2000-01-11	Synthesis of sulfurized 2'-substituted oligonucleotides
PCT/US2001/000715 WO2001051502A1 (fr)	2000-01-11	2001-01-10	Synthese amelioree d'oligonucleotides soufres

Publications (1)

Publication Number	Publication Date
AU2001227768A1 true AU2001227768A1 (en)	2001-07-24

Family

ID=23912113

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU2001227768A Abandoned AU2001227768A1 (en)	2000-01-11	2001-01-10	Improved synthesis of sulfurized oligonucleotides

Country Status (7)

Country	Link
US (4)	US6242591B1 (fr)
EP (1)	EP1246833A4 (fr)
JP (1)	JP2004500371A (fr)
KR (1)	KR20030032911A (fr)
AU (1)	AU2001227768A1 (fr)
CA (1)	CA2395765A1 (fr)
WO (1)	WO2001051502A1 (fr)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6242591B1 (en) *	1997-10-15	2001-06-05	Isis Pharmaceuticals, Inc.	Synthesis of sulfurized 2'-substituted oligonucleotides
US20030087256A1 (en) *	2001-04-06	2003-05-08	Micrologix Biotech Inc.	Thiophosphate nucleic acid-based compounds
US7169916B2 (en) *	2002-04-01	2007-01-30	Isis Pharmaceuticals, Inc.	Chloral-free DCA in oligonucleotide synthesis
US7002006B2 (en) *	2003-02-12	2006-02-21	Isis Pharmaceuticals, Inc.	Protection of nucleosides
US20040198640A1 (en) *	2003-04-02	2004-10-07	Dharmacon, Inc.	Stabilized polynucleotides for use in RNA interference
US7276599B2 (en) *	2003-06-02	2007-10-02	Isis Pharmaceuticals, Inc.	Oligonucleotide synthesis with alternative solvents
CA2538252C (fr) *	2003-09-18	2014-02-25	Isis Pharmaceuticals, Inc.	4'-thionucleosides et composes d'oligomeres
ES2535033T3 (es)	2003-11-13	2015-05-04	Isis Pharmaceuticals, Inc.	Derivados de 5,6 dihidroxi isoindol como conectores para la síntesis de oligómeros en fase sólida
KR101147147B1 (ko) *	2004-04-01	2012-05-25	머크 샤프 앤드 돔 코포레이션	Ｒｎａ 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
WO2005097817A2 (fr)	2004-04-05	2005-10-20	Alnylam Pharmaceuticals, Inc.	Procedes et reactifs pour la synthese et la purification d'oligonucleotides
WO2006023880A2 (fr) *	2004-08-23	2006-03-02	Isis Pharmaceuticals, Inc.	Composes et procedes permettant de caracteriser les oligonucleotides
US7923207B2 (en)	2004-11-22	2011-04-12	Dharmacon, Inc.	Apparatus and system having dry gene silencing pools
US7935811B2 (en) *	2004-11-22	2011-05-03	Dharmacon, Inc.	Apparatus and system having dry gene silencing compositions
US7923206B2 (en) *	2004-11-22	2011-04-12	Dharmacon, Inc.	Method of determining a cellular response to a biological agent
JP5352462B2 (ja)	2006-09-22	2013-11-27	ダーマコン，インコーポレイテッド	二本鎖オリゴヌクレオチド複合体、ｒｎａ干渉による遺伝子サイレンシング方法、および医薬品組成物
US8188060B2 (en)	2008-02-11	2012-05-29	Dharmacon, Inc.	Duplex oligonucleotides with enhanced functionality in gene regulation
US7723528B2 (en) *	2008-06-05	2010-05-25	Am Chemicals Llc	Sulfur transfer reagents for oligonucleotide synthesis
EP2937358B1 (fr) *	2008-06-23	2018-09-19	Antisense Therapeutics Ltd	Procédés pour traiter la sclérose en plaques au moyen d'oligonucléotides antisens
US8552175B2 (en)	2009-04-23	2013-10-08	A.M. Chemicals, Inc.	Sulfur transfer reagents for oligonucleotide synthesis
US8309710B2 (en)	2009-06-29	2012-11-13	Agilent Technologies, Inc.	Use of N-alkyl imidazole for sulfurization of oligonucleotides with an acetyl disulfide
US8642755B2 (en) *	2009-06-30	2014-02-04	Agilent Technologies, Inc.	Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
CN103068982B (zh) *	2010-07-14	2017-06-09	库尔纳公司	通过抑制盘状大同系物(dlg)的天然反义转录物而治疗dlg相关疾病
WO2012059510A1 (fr)	2010-11-02	2012-05-10	Girindus America, Inc.	Maîtrise de la contrepression au cours d'une synthèse en phase solide sur supports polymères
CA3163857A1 (fr)	2020-01-15	2021-07-22	Weimin Wang	Acides nucleiques phosphonate de 4'-o-methylene et analogues de ceux-ci

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3923763A (en) *	1972-09-15	1975-12-02	Petro Tex Chem Corp	Novel sulfur compound modifiers for chloroprene polymerization
JPS5442617B2 (fr)	1974-12-28	1979-12-15
US4959463A (en) *	1985-10-15	1990-09-25	Genentech, Inc.	Intermediates
NL8902521A (nl)	1989-10-11	1991-05-01	Rijksuniversiteit	Werkwijze voor het bereiden van fosforothioaatesters.
US5151510A (en) *	1990-04-20	1992-09-29	Applied Biosystems, Inc.	Method of synethesizing sulfurized oligonucleotide analogs
GB9127243D0 (en)	1991-12-23	1992-02-19	King S College London	Synthesis of phosphorothioate analogues of oligonucleotides
US5574146A (en)	1994-08-30	1996-11-12	Beckman Instruments, Inc.	Oligonucleotide synthesis with substituted aryl carboxylic acids as activators
US6001555A (en) *	1994-09-23	1999-12-14	The United States Of America As Represented By The Department Of Health And Human Services	Method for identifying and using compounds that inactivate HIV-1 and other retroviruses by attacking highly conserved zinc fingers in the viral nucleocapsid protein
US5902881A (en) *	1997-03-03	1999-05-11	Isis Pharmaceuticals, Inc.	Reagent useful for synthesizing sulfurized oligonucleotide analogs
US6114519A (en) *	1997-10-15	2000-09-05	Isis Pharmaceuticals, Inc.	Synthesis of sulfurized oligonucleotides
US6242591B1 (en) *	1997-10-15	2001-06-05	Isis Pharmaceuticals, Inc.	Synthesis of sulfurized 2'-substituted oligonucleotides
US6114518A (en) *	1999-09-30	2000-09-05	Becton, Dickinson And Company	Synthesis and use of labelled phosphoramidite compositions

2000
- 2000-01-11 US US09/481,486 patent/US6242591B1/en not_active Expired - Lifetime
2001
- 2001-01-10 AU AU2001227768A patent/AU2001227768A1/en not_active Abandoned
- 2001-01-10 US US10/181,200 patent/US7227015B2/en not_active Expired - Fee Related
- 2001-01-10 KR KR1020027008933A patent/KR20030032911A/ko not_active Withdrawn
- 2001-01-10 JP JP2001551086A patent/JP2004500371A/ja not_active Withdrawn
- 2001-01-10 EP EP01901916A patent/EP1246833A4/fr not_active Withdrawn
- 2001-01-10 CA CA002395765A patent/CA2395765A1/fr not_active Abandoned
- 2001-01-10 WO PCT/US2001/000715 patent/WO2001051502A1/fr not_active Ceased
2004
- 2004-05-17 US US10/847,502 patent/US7378516B2/en not_active Expired - Fee Related
2008
- 2008-04-21 US US12/106,889 patent/US7723511B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
WO2001051502A1 (fr)	2001-07-19
EP1246833A4 (fr)	2003-04-09
EP1246833A1 (fr)	2002-10-09
US20050165226A1 (en)	2005-07-28
JP2004500371A (ja)	2004-01-08
US20080293929A1 (en)	2008-11-27
CA2395765A1 (fr)	2001-07-19
US7227015B2 (en)	2007-06-05
US7723511B2 (en)	2010-05-25
US6242591B1 (en)	2001-06-05
US7378516B2 (en)	2008-05-27
KR20030032911A (ko)	2003-04-26
US20030212267A1 (en)	2003-11-13

Publication	Publication Date	Title
AU2001227768A1 (en)	2001-07-24	Improved synthesis of sulfurized oligonucleotides
AU2792499A (en)	1999-09-15	Improved methods for synthesis of oligonucleotides
AU3771100A (en)	2000-10-16	Synthesis of epothilones
AU2002332090A1 (en)	2003-05-06	Efficient synthesis of protein-oligonucleotide conjugates
AU2001284697A1 (en)	2002-02-18	Method of synthesizing diketopiperazines
AU2002211542A1 (en)	2002-04-15	Combinatorial synthesis system
AU2001272904A1 (en)	2002-03-22	Synthesis of 17f labeled fluoroalkanes
AU2001237992A1 (en)	2001-08-07	Purification of oligonucleotides
GB0100628D0 (en)	2001-02-21	Synthesis of tetramethylammonium hydroxide
AU2001241539A1 (en)	2001-08-27	Synthesis of resveratrol
AU2001269685A1 (en)	2001-11-12	Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
MXPA03000914A (es)	2003-10-06	Sintesis de (r)-3-(4 -bromobencil) -1-(3, 5-diclorofenil) -5-yodo-3 -metil-1-h -imidazo[1, 2-a]imidazol -2-ona.
AU2002316593A1 (en)	2003-01-21	Solid phase synthesis of arylretinamides
AU2001253143A1 (en)	2001-11-20	Synthesis of cyclohexene dimethanol compounds
AU2001277728A1 (en)	2002-02-25	Biphenylcarboxamidoisoindoline compounds, processes for the preparation of the same and intermediates for the synthesis thereof
AU2402600A (en)	2000-07-24	Pentafulvene synthesis
AUPR197000A0 (en)	2001-01-11	Synthesis of small particles
GB0014876D0 (en)	2000-08-09	Chemical synthesis
GB0004699D0 (en)	2000-04-19	Chemical synthesis
AU2002236882A1 (en)	2002-08-19	Improved methods for synthesis of oligonucleotides
AU3739300A (en)	2000-10-09	Synthesis of vapol ligands
GB0002333D0 (en)	2000-03-22	Compound synthesis
AU2001287422A1 (en)	2002-03-04	Synthesis of complex carbohydrates
AU2001259381A1 (en)	2001-12-11	Synthesis of diphosphinoarenes
AU2001249683A1 (en)	2001-10-08	Synthesis of thiazolium compounds