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AR125200A2 - Secuencia de ácidos nucleicos para la detección de planta de soja tolerante a herbicida dbn9004 y su método de detección - Google Patents

Secuencia de ácidos nucleicos para la detección de planta de soja tolerante a herbicida dbn9004 y su método de detección

Info

Publication number
AR125200A2
AR125200A2 ARP220100680A ARP220100680A AR125200A2 AR 125200 A2 AR125200 A2 AR 125200A2 AR P220100680 A ARP220100680 A AR P220100680A AR P220100680 A ARP220100680 A AR P220100680A AR 125200 A2 AR125200 A2 AR 125200A2
Authority
AR
Argentina
Prior art keywords
detection
dbn9004
soybean plant
sequence
detection method
Prior art date
Application number
ARP220100680A
Other languages
English (en)
Inventor
Dengyuan Wang
Caihong Yu
Chengwei Zhang
Chao Han
Xiaojiao Li
Ziqin Jiang
Liangjun Zhang
Zhujun Wu
Kangle Tian
Xiaoming Bao
Original Assignee
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Biotechnology Co Ltd
Publication of AR125200A2 publication Critical patent/AR125200A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01183—Phosphinothricin acetyltransferase (2.3.1.183)
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    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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Abstract

La presente invención se refiere a una secuencia de ácidos nucleicos para la detección de una planta de soja tolerante a herbicida DBN9004 y su método de detección, la secuencia de ácidos nucleicos de la planta de soja comprende la SEQ ID Nº 1 o su secuencia complementaria o la SEQ ID Nº 2 o su secuencia complementaria. El evento de soja transgénica DBN9004 de la presente invención tiene mejor tolerancia a un herbicida de glifosato y un herbicida de glufosinato y no tiene influencia sobre el rendimiento y el método de detección puede identificar de modo preciso y rápido si una molécula de ADN del evento de soja transgénico DBN9004 está contenida en una muestra biológica.
ARP220100680A 2016-06-18 2022-03-22 Secuencia de ácidos nucleicos para la detección de planta de soja tolerante a herbicida dbn9004 y su método de detección AR125200A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610440310.4A CN106086011B (zh) 2016-06-18 2016-06-18 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法

Publications (1)

Publication Number Publication Date
AR125200A2 true AR125200A2 (es) 2023-06-21

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Family Applications (2)

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ARP170101647A AR108751A1 (es) 2016-06-18 2017-06-15 Secuencia de ácidos nucleicos para la detección de planta de soja tolerante a herbicida dbn9004 y su método de detección
ARP220100680A AR125200A2 (es) 2016-06-18 2022-03-22 Secuencia de ácidos nucleicos para la detección de planta de soja tolerante a herbicida dbn9004 y su método de detección

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EP (1) EP3473719A4 (es)
JP (1) JP7039493B2 (es)
KR (1) KR102296563B1 (es)
CN (1) CN106086011B (es)
AR (2) AR108751A1 (es)
AU (1) AU2017284519B9 (es)
BR (1) BR112018001306B1 (es)
MX (1) MX2018015213A (es)
RU (1) RU2743397C2 (es)
UY (1) UY37299A (es)
WO (1) WO2017215328A1 (es)
ZA (1) ZA201806079B (es)

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US12213421B2 (en) 2019-08-09 2025-02-04 Beijing Dabeinong Biotechnology Co. Ltd. Nucleic acid sequence for detecting soybean plant DBN8002 and detection method therefor
CN111247255B (zh) * 2019-08-09 2023-06-27 北京大北农生物技术有限公司 用于检测大豆植物dbn8007的核酸序列及其检测方法
CN112831584B (zh) * 2021-01-27 2023-06-06 隆平生物技术(海南)有限公司 转基因玉米事件lp007-2及其检测方法
EP4484580A4 (en) 2022-02-21 2025-12-03 Beijing Dabeinong Biotechnology Co Ltd Nucleic acid sequence for the detection of the Glycine Max plant DBN8205 and its detection method
CN116103332B (zh) * 2022-09-07 2025-09-23 杭州瑞丰生物科技有限公司 转基因大豆事件cal16及其检测方法
CN116732215A (zh) * 2023-03-13 2023-09-12 北京大北农生物技术有限公司 用于检测玉米植物dbn9229的核酸序列及其检测方法
CN116656869B (zh) * 2023-07-24 2023-09-22 捷康生物科技(海南)有限公司 转基因大豆事件jk1001-2及其检测方法
CN116656870B (zh) * 2023-07-25 2023-09-22 隆平生物技术(海南)有限公司 转基因大豆事件lp086-3及其检测方法
CN121249963A (zh) * 2025-12-04 2026-01-02 捷康生物科技(海南)有限公司 转基因大豆事件jk1013-2及其检测方法

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CN106086011B (zh) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法

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KR20190003994A (ko) 2019-01-10
KR102296563B1 (ko) 2021-08-31
AU2017284519A1 (en) 2018-12-13
WO2017215328A1 (zh) 2017-12-21
AU2017284519B9 (en) 2021-03-25
UY37299A (es) 2017-08-31
BR112018001306A2 (pt) 2020-12-01
RU2018141490A3 (es) 2020-07-20
AU2017284519B2 (en) 2021-03-04
AR108751A1 (es) 2018-09-19
MX2018015213A (es) 2019-04-25
CN106086011B (zh) 2019-10-18
RU2743397C2 (ru) 2021-02-18
BR112018001306B1 (pt) 2024-03-12
EP3473719A1 (en) 2019-04-24
ZA201806079B (en) 2019-06-26
JP7039493B2 (ja) 2022-03-22
CN106086011A (zh) 2016-11-09
EP3473719A4 (en) 2020-01-15
RU2018141490A (ru) 2020-07-20
JP2019527042A (ja) 2019-09-26

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