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AR104438A1 - Planta de maíz dbn9868 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección - Google Patents

Planta de maíz dbn9868 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección

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Publication number
AR104438A1
AR104438A1 ARP160101207A ARP160101207A AR104438A1 AR 104438 A1 AR104438 A1 AR 104438A1 AR P160101207 A ARP160101207 A AR P160101207A AR P160101207 A ARP160101207 A AR P160101207A AR 104438 A1 AR104438 A1 AR 104438A1
Authority
AR
Argentina
Prior art keywords
dbn9868
nucleic acid
detection
corn plant
sequence
Prior art date
Application number
ARP160101207A
Other languages
English (en)
Inventor
Bao Xiaoming
Xu Liang
Xiong Guanjun
Xia Zuling
Tang Bo
Wei Xuesong
Jiao Guowei
Ding Derong
Zhang Chengwei
Liu Haili
Guo Mingxin
Kang Yuejing
Original Assignee
Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Tech Group, Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Tech Group
Publication of AR104438A1 publication Critical patent/AR104438A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente se refiere a una secuencia de ácido nucleico para detectar la planta de maíz DBN9868 tolerante a herbicidas y un método para su detección, donde la secuencia de ácido nucleico de la planta de maíz comprende la SEQ ID Nº 1 o la secuencia complementaria de la misma, o la SEQ ID Nº 2 o la secuencia complementaria de la misma. El evento de maíz transgénico DBN9868 de la presente tiene mejor tolerancia al herbicida glifosato y al herbicida glufosinato sin afectar el rendimiento, y el método de detección puede detectar precisa y rápidamente si una muestra biológica comprende la molécula de ADN del evento de maíz transgénico DBN9868.
ARP160101207A 2015-04-30 2016-04-28 Planta de maíz dbn9868 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección AR104438A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510219912.2A CN104878096B (zh) 2015-04-30 2015-04-30 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法

Publications (1)

Publication Number Publication Date
AR104438A1 true AR104438A1 (es) 2017-07-19

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CN (1) CN104878096B (es)
AR (1) AR104438A1 (es)
WO (1) WO2016173539A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104878096B (zh) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法
CN109868273B (zh) * 2019-04-09 2023-02-03 北京大北农生物技术有限公司 用于检测玉米植物dbn9501的核酸序列及其检测方法
CN111247255B (zh) * 2019-08-09 2023-06-27 北京大北农生物技术有限公司 用于检测大豆植物dbn8007的核酸序列及其检测方法
CN112831584B (zh) * 2021-01-27 2023-06-06 隆平生物技术(海南)有限公司 转基因玉米事件lp007-2及其检测方法
CN112852991B (zh) * 2021-01-27 2023-08-04 隆平生物技术(海南)有限公司 转基因玉米事件lp007-7及其检测方法
CN113201531B (zh) * 2021-04-27 2023-06-27 隆平生物技术(海南)有限公司 转基因玉米事件lw2-1及其检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020144A2 (en) * 1996-11-07 1998-05-14 Zeneca Limited Herbicide resistant plants
BRPI0100752B1 (pt) * 2000-06-22 2015-10-13 Monsanto Co moléculas e pares de moléculas de dna, processos para detectar molécula de dna e para criar um traço tolerante a glifosato em plantas de milho, bem como kit de detecção de dna
AU2007267586B2 (en) * 2006-05-26 2012-04-05 Monsanto Technology, Llc Corn plant and seed corresponding to transgenic event MON89034 and methods for detection and use thereof
CN101641443A (zh) * 2006-10-30 2010-02-03 先锋高级育种国际公司 玉米事件dp-098140-6，鉴定和/或检测其的组合物和方法
MD20140016A2 (ro) * 2011-07-28 2014-07-31 Genective Transformare VCO-Ø1981-5 a porumbului pentru conferirea toleranţei la glifosat, set şi metodă de detectare a acesteia
KR102085131B1 (ko) * 2012-06-25 2020-03-05 다우 아그로사이언시즈 엘엘씨 곤충 저항성 및 제초제 내성 대두 이벤트 pDAB9582.816.15.1
CN104878096B (zh) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法

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WO2016173539A1 (zh) 2016-11-03
CN104878096A (zh) 2015-09-02
CN104878096B (zh) 2019-01-15

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