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AR113728A1 - Sistemas crispr / cpf1 optimizados en planta - Google Patents

Sistemas crispr / cpf1 optimizados en planta

Info

Publication number
AR113728A1
AR113728A1 ARP190100059A ARP190100059A AR113728A1 AR 113728 A1 AR113728 A1 AR 113728A1 AR P190100059 A ARP190100059 A AR P190100059A AR P190100059 A ARP190100059 A AR P190100059A AR 113728 A1 AR113728 A1 AR 113728A1
Authority
AR
Argentina
Prior art keywords
plant
sequence
cells
rna
cpf1
Prior art date
Application number
ARP190100059A
Other languages
English (en)
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR113728A1 publication Critical patent/AR113728A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/02—Amaranthaceae or Chenopodiaceae, e.g. beet or spinach
    • A01H6/024—Beta vulgaris [beet]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente está relacionada con sistemas optimizados para la edición genómica de células eucariotas, preferentemente células vegetales. También se proporciona un sistema de entrega en planta que comprende al menos una enzima Cpf1, o un constructo optimizado en planta que la codifica, para que se combine sinérgicamente con un sistema Cpf1 guiado por ARN. Se proporciona además un sistema Cpf1 guiado por ARN flanqueado por una secuencia de ribozimas cabeza de martillo en el extremo 5’ y por una secuencia de ribozimas tipo Virus de la Hepatitis Delta (VHD) derivadas de planta en el extremo 3’, o incrustado dentro de una región codificadora o no codificadora, de una secuencia que codifica una secuencia marco. También se proporcionan secuencias de ribozimas tipo HDV derivadas de planta. Asimismo se proporcionan métodos para edición genómica mejorada y el uso de los diversos sistemas proporcionados en la presente para obtener plantas, células, tejidos, órganos trasformados o su progenie, o un material de planta, modificado en forma dirigida inclusive en sitios diana de difícil acceso.
ARP190100059A 2018-01-11 2019-01-11 Sistemas crispr / cpf1 optimizados en planta AR113728A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201862616136P 2018-01-11 2018-01-11

Publications (1)

Publication Number Publication Date
AR113728A1 true AR113728A1 (es) 2020-06-03

Family

ID=65041730

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190100059A AR113728A1 (es) 2018-01-11 2019-01-11 Sistemas crispr / cpf1 optimizados en planta

Country Status (7)

Country Link
US (2) US12098374B2 (es)
EP (1) EP3737691A1 (es)
CN (1) CN111836825A (es)
AR (1) AR113728A1 (es)
BR (1) BR112020014017A2 (es)
CA (1) CA3088052A1 (es)
WO (1) WO2019138052A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182370B (zh) * 2018-08-03 2022-06-17 浙江大学 一种植物多基因表达载体、转化子及其应用
EP3927820A4 (en) 2019-02-22 2024-03-27 Integrated DNA Technologies, Inc. LACHNOSPIRACEAE BACTERIUM ND2006 CAS12A MUTANT GENES AND POLYPEPTIDES ENCODED BY THEM
EP4045643A4 (en) 2019-10-17 2023-11-08 Pairwise Plants Services, Inc. CAS12A NUCLEASE VARIANTS AND METHODS OF MAKING AND USING THE SAME
US20210130838A1 (en) * 2019-11-05 2021-05-06 University Of Maryland, College Park SYSTEMS AND METHODS FOR PLANT GENOME EDITING USING CAS 12a ORTHOLOGS
US20230002761A1 (en) 2019-11-19 2023-01-05 Benson Hill, Inc. Anti-bacterial crispr compositions and methods
CN111647618A (zh) * 2020-01-15 2020-09-11 温州医科大学 一种新型基因组编辑工具（Lb2Cas12a-RVR）及其构建方法和应用方法
US12012433B1 (en) 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
WO2021175289A1 (zh) * 2020-03-04 2021-09-10 中国科学院遗传与发育生物学研究所 多重基因组编辑方法和系统
WO2021222703A2 (en) 2020-05-01 2021-11-04 Integrated Dna Technologies, Inc. Lachnospiraceae sp. cas12a mutants with enhanced cleavage activity at non-canonical tttt protospacer adjacent motifs
CN117965543A (zh) 2022-03-02 2024-05-03 苏州科锐迈德生物医药科技有限公司 一种重组核酸分子及其在制备环状rna中的应用
CN115851784B (zh) * 2022-08-02 2023-06-27 安徽农业大学 一种利用Lbcpf1变体构建的植物胞嘧啶碱基编辑系统及其应用
CN116103266B (zh) * 2022-12-28 2025-09-30 武汉艾迪晶生物科技有限公司 用于水稻基因编辑的mad7-nls融合蛋白、载体及其应用
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A
CN119193690A (zh) * 2024-11-14 2024-12-27 中国农业科学院植物保护研究所 一种基于CRISPR/LbCas12a系统抗多种双生病毒侵染载体及构建方法和应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789317B (zh) 2014-08-06 2024-02-23 基因工具股份有限公司 使用空肠弯曲杆菌crispr/cas系统衍生的rna引导的工程化核酸酶的基因编辑
WO2016073433A1 (en) * 2014-11-06 2016-05-12 E. I. Du Pont De Nemours And Company Peptide-mediated delivery of rna-guided endonuclease into cells
JP7011590B2 (ja) * 2015-10-12 2022-02-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 細胞内での遺伝子組換えおよび相同組換えの増加のための保護ｄｎａ鋳型および使用方法
EP3199632A1 (en) * 2016-01-26 2017-08-02 ACIB GmbH Temperature-inducible crispr/cas system
KR20260004568A (ko) 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
CN109312317A (zh) 2016-06-14 2019-02-05 先锋国际良种公司 Cpf1内切核酸酶用于植物基因组修饰的用途
ES2981548T3 (es) 2016-06-20 2024-10-09 Keygene Nv Método para la alteración dirigida del ADN en células vegetales
CN107012164B (zh) * 2017-01-11 2023-03-03 电子科技大学 CRISPR/Cpf1植物基因组定向修饰功能单元、包含该功能单元的载体及其应用

Also Published As

Publication number Publication date
US20210163968A1 (en) 2021-06-03
CA3088052A1 (en) 2019-07-18
CN111836825A (zh) 2020-10-27
US20240409949A1 (en) 2024-12-12
BR112020014017A2 (pt) 2020-12-08
WO2019138052A1 (en) 2019-07-18
EP3737691A1 (en) 2020-11-18
US12098374B2 (en) 2024-09-24

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