AR109017A1 - Métodos para utilizar polinucleótidos largos de adn simple cadena como cebadores (primers) en ensayos de pcr - Google Patents
Métodos para utilizar polinucleótidos largos de adn simple cadena como cebadores (primers) en ensayos de pcrInfo
- Publication number
- AR109017A1 AR109017A1 ARP170101919A ARP170101919A AR109017A1 AR 109017 A1 AR109017 A1 AR 109017A1 AR P170101919 A ARP170101919 A AR P170101919A AR P170101919 A ARP170101919 A AR P170101919A AR 109017 A1 AR109017 A1 AR 109017A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- primers
- polyucleotides
- methods
- single chain
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 108091092878 Microsatellite Proteins 0.000 abstract 2
- 108091026890 Coding region Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 238000002944 PCR assay Methods 0.000 abstract 1
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para realizar un ensayo de PCR que comprende los siguientes pasos: a) obtener una muestra de ácido nucleico; b) hibridizar dicha muestra de ácido nucleico a uno o más pares de primers donde al menos un primer consiste en un polinucleótido de ADN simple cadena de 60 o más nucleótidos; c) someter dicha muestra de ácido nucleico a una PCR donde el medio de la muestra de reacción contiene al menos uno de dichos primers; y d) detectar la longitud de los productos amplificados. El ácido nucleico amplificado puede contener cualquier secuencia o múltiples secuencias de repeticiones cortas en tándem (STRs, Short Tandem Repeats), genes o cualquier región codificante que tenga una ubicación definida en el genoma. Las muestras de ácido nucleico preferidas para ser amplificadas están degradadas o fragmentadas y contienen uno o más marcadores genéticos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662361184P | 2016-07-12 | 2016-07-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR109017A1 true AR109017A1 (es) | 2018-10-17 |
Family
ID=60941961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP170101919A AR109017A1 (es) | 2016-07-12 | 2017-07-11 | Métodos para utilizar polinucleótidos largos de adn simple cadena como cebadores (primers) en ensayos de pcr |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US10822647B2 (es) |
| EP (1) | EP3325671B1 (es) |
| CN (1) | CN109477139B (es) |
| AR (1) | AR109017A1 (es) |
| WO (1) | WO2018011101A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200005894A1 (en) * | 2018-06-29 | 2020-01-02 | Rady Children's Hospital Research Center | Method and system for sample identity assurance |
| JP2025063392A (ja) * | 2023-10-04 | 2025-04-16 | 株式会社日立製作所 | 塩基検出用長鎖ｓｓＤＮＡプライマー |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3834636A1 (de) | 1988-10-11 | 1990-04-19 | Max Planck Gesellschaft | Verfahren zur analyse von laengenpolymorphismen in dna-bereichen |
| US5582979A (en) | 1989-04-21 | 1996-12-10 | Marshfield Clinic | Length polymorphisms in (dC-dA)n.(dG-dT)n sequences and method of using the same |
| US5364759B2 (en) | 1991-01-31 | 1999-07-20 | Baylor College Medicine | Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats |
| US6479235B1 (en) | 1994-09-30 | 2002-11-12 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| DE10113265B4 (de) * | 2001-03-16 | 2018-03-08 | Qiagen Gmbh | Verwendung einer stabilisierten Nukleinsäure zur Herstellung eines Proteins |
| US6743905B2 (en) | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| CN101341258A (zh) * | 2004-11-03 | 2009-01-07 | 第三次浪潮技术公司 | 单步检测测定法 |
| CN101210240B (zh) * | 2007-12-25 | 2011-11-16 | 天津大学 | 大引物pcr进行定点突变的方法 |
| CN101818192B (zh) * | 2009-08-27 | 2013-03-20 | 基点认知技术(北京)有限公司 | 20个短串联重复序列的复合扩增试剂盒 |
| EP2872650A2 (en) * | 2012-07-13 | 2015-05-20 | Life Technologies Corporation | Human identifiation using a panel of snps |
| US9556482B2 (en) * | 2013-07-03 | 2017-01-31 | The United States Of America, As Represented By The Secretary Of Commerce | Mouse cell line authentication |
| EP2860262A1 (en) | 2013-10-09 | 2015-04-15 | Universität Heidelberg | Method for the quantitative analysis of nucleic acid fragmentation and amplifiability |
| CN106164298B (zh) * | 2014-02-18 | 2020-02-21 | 伊鲁米那股份有限公司 | 用于dna谱系分析的方法及组合物 |
-
2016
- 2016-10-03 US US15/283,851 patent/US10822647B2/en active Active
-
2017
- 2017-07-10 EP EP17755066.2A patent/EP3325671B1/en active Active
- 2017-07-10 CN CN201780042965.9A patent/CN109477139B/zh not_active Expired - Fee Related
- 2017-07-10 WO PCT/EP2017/067189 patent/WO2018011101A1/en not_active Ceased
- 2017-07-11 AR ARP170101919A patent/AR109017A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN109477139B (zh) | 2022-07-29 |
| CN109477139A (zh) | 2019-03-15 |
| US20180016621A1 (en) | 2018-01-18 |
| WO2018011101A1 (en) | 2018-01-18 |
| US10822647B2 (en) | 2020-11-03 |
| EP3325671B1 (en) | 2022-10-26 |
| EP3325671A1 (en) | 2018-05-30 |
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