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MY174088A - A method of detecting porcine markers for identifying pork content in a sample - Google Patents

A method of detecting porcine markers for identifying pork content in a sample

Info

Publication number
MY174088A
MY174088A MYPI2014702291A MYPI2014702291A MY174088A MY 174088 A MY174088 A MY 174088A MY PI2014702291 A MYPI2014702291 A MY PI2014702291A MY PI2014702291 A MYPI2014702291 A MY PI2014702291A MY 174088 A MY174088 A MY 174088A
Authority
MY
Malaysia
Prior art keywords
sample
markers
comprises seq
porcine
reverse primer
Prior art date
Application number
MYPI2014702291A
Inventor
S Rozhdestvensky Timofey
Brosius Juergen
A Raabe Carsten
Thean Hock Tang
Li Pin Lee
Aminah Ahmed Siti
Original Assignee
Univ Sains Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sains Malaysia filed Critical Univ Sains Malaysia
Priority to MYPI2014702291A priority Critical patent/MY174088A/en
Priority to PCT/MY2015/050016 priority patent/WO2016028138A1/en
Publication of MY174088A publication Critical patent/MY174088A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method of detecting porcine markers for identifying a pork content in a test sample, comprising the steps of: extracting deoxyribonucleic acid (DNA) from the sample; performing polymerase chain reaction (PCR) on the extracted DNA using two pairs of primers, including a first forward primer and a first reverse primer for amplifying a target porcine DNA sequence, and a second forward primer and a second reverse primer (as an internal control for amplifying a target gene sequence; then subjecting the amplified sequence to electrophoresis to detect the presence of porcine markers in the sample; characterized in that: the first forward primer comprises SEQ ID N0:1, the first reverse primer comprises SEQ ID N0:2, the second forward primer comprises SEQ ID N0:3, and the second reverse primer comprises SEQ ID N0:4.
MYPI2014702291A 2014-08-18 2014-08-18 A method of detecting porcine markers for identifying pork content in a sample MY174088A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2014702291A MY174088A (en) 2014-08-18 2014-08-18 A method of detecting porcine markers for identifying pork content in a sample
PCT/MY2015/050016 WO2016028138A1 (en) 2014-08-18 2015-03-19 A method of detecting porcine markers for identifying pork content in a sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2014702291A MY174088A (en) 2014-08-18 2014-08-18 A method of detecting porcine markers for identifying pork content in a sample

Publications (1)

Publication Number Publication Date
MY174088A true MY174088A (en) 2020-03-09

Family

ID=55350999

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2014702291A MY174088A (en) 2014-08-18 2014-08-18 A method of detecting porcine markers for identifying pork content in a sample

Country Status (2)

Country Link
MY (1) MY174088A (en)
WO (1) WO2016028138A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108118086A (en) * 2018-01-30 2018-06-05 西北民族大学 For detecting RPA primers, probe and the method for pork content in meat products
CN111440881B (en) * 2020-05-27 2023-11-03 兰州海关技术中心 Primer group, kit and detection method for detecting pork and application
CN115927651A (en) * 2022-07-22 2023-04-07 中国食品药品检定研究院 Primer and method for detecting swine-origin DNA
CN118033122A (en) * 2024-02-24 2024-05-14 华北理工大学 Preparation method of flow strip for simultaneous identification of meat-derived components by nanoparticle aggregation and color development

Also Published As

Publication number Publication date
WO2016028138A1 (en) 2016-02-25

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