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AR108807A1 - Métodos y composiciones para otorgar y/o mejorar la tolerancia a los herbicidas utilizando protoporfirinógeno oxidasa o una variante de esta - Google Patents

Métodos y composiciones para otorgar y/o mejorar la tolerancia a los herbicidas utilizando protoporfirinógeno oxidasa o una variante de esta

Info

Publication number
AR108807A1
AR108807A1 ARP170101674A ARP170101674A AR108807A1 AR 108807 A1 AR108807 A1 AR 108807A1 AR P170101674 A ARP170101674 A AR P170101674A AR P170101674 A ARP170101674 A AR P170101674A AR 108807 A1 AR108807 A1 AR 108807A1
Authority
AR
Argentina
Prior art keywords
seq
amino acid
polypeptide
polypeptide according
algae
Prior art date
Application number
ARP170101674A
Other languages
English (en)
Inventor
Myoung Hong
- Park Joonghyuk Ki
Young Ock Ahn
Yunjung Han
Joonseon Yoon
Soon-Kee Sung
Original Assignee
Farmhannong Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmhannong Co Ltd filed Critical Farmhannong Co Ltd
Publication of AR108807A1 publication Critical patent/AR108807A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Facsimiles In General (AREA)

Abstract

Reivindicación 1: Un polipéptido a seleccionar a partir de los siguientes: un polipéptido que comprende una secuencia de aminoácidos en donde al menos uno a seleccionar a partir del conjunto que consiste en N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, y I408 de la secuencia de aminoácidos de SEC ID Nº 2, considerado en forma independiente, es eliminado o es sustituido por un aminoácido que se selecciona a partir del conjunto que consiste en M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), F(Phe), P(Pro), W(Trp), N(Asp), Q(Gln), G(Gly), Y(Tyr), D(Asp), E(Glu), R(Arg), H(His), y K(Lys), y es diferente del aminoácido original en la correspondiente posición en el tipo salvaje; un polipéptido que comprende una secuencia de aminoácidos en donde al menos uno a seleccionar a partir del conjunto que consiste en R101, F171, V175, A177, G194, P316, V318, F337, L340, I353, y F373 de la secuencia de aminoácidos de SEC ID Nº 4, considerado en forma independiente, es eliminado o es sustituido por un aminoácido que se selecciona a partir del conjunto que consiste en M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), F(Phe), P(Pro), W(Trp), N(Asp), Q(Gln), G(Gly), Y(Tyr), D(Asp), E(Glu), R(Arg), H(His), y K(Lys), y es diferente del aminoácido en la correspondiente posición en el tipo salvaje; y un polipéptido que consiste en una secuencia de aminoácidos que posee 95% o más de homología con respecto a las secuencias de aminoácidos de dichos polipéptidos. Reivindicación 5: Un polinucleótido que codifica el polipéptido conforme a una cualquiera de las reivindicaciones 1 a 4. Reivindicación 8: Una composición para conferir o potenciar la tolerancia a los herbicidas de una planta o alga, la cual comprende al menos uno a seleccionar a partir del conjunto que consiste en el polipéptido de la SEC ID Nº 2; el polipéptido de la SEC ID Nº 4; el polipéptido conforme a una cualquiera de las reivindicaciones 1 a 4; un polinucleótido que codifica el polipéptido; un vector recombinante que comprende el polinucleótido; y una célula recombinante que comprende el vector recombinante. Reivindicación 16: Un transformante de una planta o alga que posee la tolerancia a los herbicidas, o un clon o la progenie del mismo, la cual comprende el polipéptido conforme a la SEC ID Nº 2, el polipéptido conforme a la SEC ID Nº 4, el polipéptido conforme a una cualquiera de las reivindicaciones 1 a 4 o un polinucleótido que lo codifica. Reivindicación 19: Un método para conferir o potenciar la tolerancia a los herbicidas de plantas o algas, siendo que el método comprende transformar algas, o una célula vegetal, protoplasto, callo, hipocótilo, semilla, cotiledón, vástago, o planta completa con el polipéptido conforme a SEC ID Nº 2, el polipéptido conforme a SEC ID Nº 4, el polipéptido conforme a una cualquiera de las reivindicaciones 1 a 4 o un polinucleótido que lo codifica. Reivindicación 23: Un método para eliminar un organismo acuático no deseado de un medio de cultivo, siendo que el método comprende: proporcionar a un medio de cultivo algas que comprenden el polipéptido conforme a SEC ID Nº 2, el polipéptido conforme a SEC ID Nº 4, el polipéptido conforme a una cualquiera de las reivindicaciones 1 a 4 o un polinucleótido que lo codifica, y aplicar al medio de cultivo una dosis efectiva de herbicida inhibidor de la protoporfirinógeno oxidasa.
ARP170101674A 2016-06-16 2017-06-16 Métodos y composiciones para otorgar y/o mejorar la tolerancia a los herbicidas utilizando protoporfirinógeno oxidasa o una variante de esta AR108807A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20160075358 2016-06-16

Publications (1)

Publication Number Publication Date
AR108807A1 true AR108807A1 (es) 2018-09-26

Family

ID=60663533

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Application Number Title Priority Date Filing Date
ARP170101674A AR108807A1 (es) 2016-06-16 2017-06-16 Métodos y composiciones para otorgar y/o mejorar la tolerancia a los herbicidas utilizando protoporfirinógeno oxidasa o una variante de esta

Country Status (16)

Country Link
US (2) US10844395B2 (es)
EP (1) EP3472184A4 (es)
JP (1) JP6891198B2 (es)
KR (3) KR101862796B1 (es)
CN (2) CN109476709A (es)
AR (1) AR108807A1 (es)
AU (1) AU2017284680B2 (es)
CA (1) CA3024985A1 (es)
CL (2) CL2018003620A1 (es)
CO (1) CO2019000095A2 (es)
IL (1) IL263413B2 (es)
MX (1) MX2018015794A (es)
PH (1) PH12018502621A1 (es)
UY (1) UY37298A (es)
WO (1) WO2017217793A1 (es)
ZA (1) ZA201807594B (es)

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WO2024175475A1 (en) 2023-02-24 2024-08-29 Syngenta Crop Protection Ag Herbicidal compositions
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BR112018076029A2 (pt) 2019-03-26
CN109476709A (zh) 2019-03-15
CN116064434B (zh) 2025-10-10
UY37298A (es) 2018-01-02
IL263413B2 (en) 2024-02-01
CL2020001807A1 (es) 2020-12-04
JP2019527538A (ja) 2019-10-03
IL263413A (en) 2018-12-31
CA3024985A1 (en) 2017-12-21
ZA201807594B (en) 2020-02-26
KR101862796B1 (ko) 2018-05-30
US20210095305A1 (en) 2021-04-01
US11466286B2 (en) 2022-10-11
AU2017284680A1 (en) 2018-12-13
CL2018003620A1 (es) 2019-03-29
KR102250017B1 (ko) 2021-05-10
US10844395B2 (en) 2020-11-24
CN116064434A (zh) 2023-05-05
KR20170142120A (ko) 2017-12-27
JP6891198B2 (ja) 2021-06-18
MX2018015794A (es) 2019-03-21
KR20190127644A (ko) 2019-11-13
AU2017284680B2 (en) 2020-04-30
KR20180000717A (ko) 2018-01-03
CO2019000095A2 (es) 2019-02-19
PH12018502621A1 (en) 2019-10-07
WO2017217793A1 (en) 2017-12-21
EP3472184A1 (en) 2019-04-24
KR102044430B1 (ko) 2019-11-14
US20190330651A1 (en) 2019-10-31
IL263413B1 (en) 2023-10-01
EP3472184A4 (en) 2019-11-20

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