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AR105862A1 - Cepas de levadura que coexpresan glucoamilasas exógenas, procedimiento de obtención y su utilización para producir bioetanol - Google Patents

Cepas de levadura que coexpresan glucoamilasas exógenas, procedimiento de obtención y su utilización para producir bioetanol

Info

Publication number
AR105862A1
AR105862A1 ARP160102643A ARP160102643A AR105862A1 AR 105862 A1 AR105862 A1 AR 105862A1 AR P160102643 A ARP160102643 A AR P160102643A AR P160102643 A ARP160102643 A AR P160102643A AR 105862 A1 AR105862 A1 AR 105862A1
Authority
AR
Argentina
Prior art keywords
glucoamylase
saccharomyces cerevisiae
var
gene
obtaining
Prior art date
Application number
ARP160102643A
Other languages
English (en)
Inventor
Thomas Benot
Bavouzet Jean
Michel - Pignede Georges
Petit Maud
Original Assignee
Lesaffre & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lesaffre & Cie filed Critical Lesaffre & Cie
Publication of AR105862A1 publication Critical patent/AR105862A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Cepas de levadura de Saccharomyces cerevisiae mejoradas, que se caracterizan porque coexpresan un gen que codifica para una glucoamilasa de origen fúngico y un gen que codifica para la glucoamilasa de Saccharomyces cerevisiae var. diastaticus. La presente divulgación se relaciona también con un método para la obtención de cepas de levadura, en donde dicho método comprende los siguientes pasos: a) modificar genéticamente una levadura de Saccharomyces cerevisiae de forma de hacerla coexpresar un gen que codifica para una glucoamilasa de origen fúngico y un gen que codifica para la glucoamilasa de Saccharomyces cerevisiae var. diastaticus; b) cultivar y fermentar la cepa que se obtuvo en el paso a) en un medio de dextrina; c) seleccionar las cepas que presenten una cinética de fermentación por lo menos igual o superior a la cepa ingresada el 9 de julio de 2015 en el marco del tratado de Budapest a la CNCM bajo el número I-4999 en las mismas condiciones. Las cepas de levadura son de interés particular en la producción de bioetanol.
ARP160102643A 2015-08-31 2016-08-30 Cepas de levadura que coexpresan glucoamilasas exógenas, procedimiento de obtención y su utilización para producir bioetanol AR105862A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1558079A FR3040395B1 (fr) 2015-08-31 2015-08-31 Souches de levure co-exprimant des glucoamylases exogenes, leur procede d'obtention et leur utilisation pour produire du bioethanol

Publications (1)

Publication Number Publication Date
AR105862A1 true AR105862A1 (es) 2017-11-15

Family

ID=54329795

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160102643A AR105862A1 (es) 2015-08-31 2016-08-30 Cepas de levadura que coexpresan glucoamilasas exógenas, procedimiento de obtención y su utilización para producir bioetanol

Country Status (9)

Country Link
US (1) US10947519B2 (es)
EP (1) EP3344763B1 (es)
CN (1) CN108603186B (es)
AR (1) AR105862A1 (es)
BR (1) BR112018004066B1 (es)
DK (1) DK3344763T3 (es)
FR (1) FR3040395B1 (es)
PL (1) PL3344763T3 (es)
WO (1) WO2017037362A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111148840B (zh) 2017-09-29 2024-08-30 丹尼斯科美国公司 改进的无甘油乙醇生产
FR3083245B1 (fr) 2018-07-02 2022-06-17 Lesaffre & Cie Souches de saccharomyces cerevisiae exprimant des enzymes glucoamylase et xylanase exogenes et leur utilisation dans la production de bioethanol
CN111334446B (zh) * 2018-12-19 2022-08-26 吉林中粮生化有限公司 一株耐高温糖化酵母菌株及其应用
CN113052271B (zh) * 2021-05-14 2022-02-15 江南大学 基于深度神经网络的生物发酵数据预测方法
WO2023079048A1 (en) * 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243256B2 (en) * 2010-04-12 2016-01-26 Stellenbosch University Biofuel production

Also Published As

Publication number Publication date
BR112018004066A2 (pt) 2018-12-11
DK3344763T3 (da) 2021-11-22
PL3344763T3 (pl) 2022-02-07
CN108603186A (zh) 2018-09-28
US10947519B2 (en) 2021-03-16
BR112018004066B1 (pt) 2023-11-28
WO2017037362A1 (fr) 2017-03-09
FR3040395B1 (fr) 2019-01-25
EP3344763A1 (fr) 2018-07-11
US20190292532A1 (en) 2019-09-26
EP3344763B1 (fr) 2021-10-06
CN108603186B (zh) 2022-01-11
FR3040395A1 (fr) 2017-03-03

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