AR098283A1 - Polinucleótido donante universal para reconocimiento de genes - Google Patents
Polinucleótido donante universal para reconocimiento de genesInfo
- Publication number
- AR098283A1 AR098283A1 ARP140104120A ARP140104120A AR098283A1 AR 098283 A1 AR098283 A1 AR 098283A1 AR P140104120 A ARP140104120 A AR P140104120A AR P140104120 A ARP140104120 A AR P140104120A AR 098283 A1 AR098283 A1 AR 098283A1
- Authority
- AR
- Argentina
- Prior art keywords
- domain
- plant cell
- site
- genome
- nuclease
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 abstract 5
- 102000040430 polynucleotide Human genes 0.000 abstract 5
- 239000002157 polynucleotide Substances 0.000 abstract 5
- 101710163270 Nuclease Proteins 0.000 abstract 4
- 230000004568 DNA-binding Effects 0.000 abstract 3
- 230000010354 integration Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000004570 RNA-binding Effects 0.000 abstract 1
- 230000027455 binding Effects 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se describe un polinucleótido donante universal que puede insertarse en ubicaciones reconocidas como objetivo en genomas de plantas para facilitar la integración rápida y de alto rendimiento de una molécula donante dentro de una ubicación genómica específica. Reivindicación 1: Un casete de donante polinucleótido que comprende un dominio de unión de nucleasa específica de un sitio, un dominio analítico, y un dominio de plásmido. Reivindicación 36: Un método para integración objetivada de un casete de donante polinucleótido dentro del genoma de una célula de planta, en donde el método comprende: a) expresar una nucleasa de unión de ADN específico de sitio, que comprende por lo menos un dominio de unión de ARN y por lo menos un dominio de nucleasa, en donde por lo menos un dominio de unión de ADN se une a un sitio objetivo dentro del genoma de la célula de planta; b) poner en contacto la célula de planta con un casete de donante polinucleótido; c) clivar el sitio objetivo dentro del genoma de la célula de planta con la nucleasa de unión de ADN especifico de sitio; y d) integrar el casete de donante polinucleótido dentro del sitio objetivo dentro del genoma de la célula de planta.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361899543P | 2013-11-04 | 2013-11-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR098283A1 true AR098283A1 (es) | 2016-05-26 |
Family
ID=53005271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP140104120A AR098283A1 (es) | 2013-11-04 | 2014-11-03 | Polinucleótido donante universal para reconocimiento de genes |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10077449B2 (es) |
| EP (1) | EP3066192B1 (es) |
| JP (1) | JP6560203B2 (es) |
| KR (1) | KR102248730B1 (es) |
| CN (1) | CN105683357B (es) |
| AR (1) | AR098283A1 (es) |
| AU (1) | AU2014341928B2 (es) |
| BR (1) | BR102014027448A8 (es) |
| CA (1) | CA2926534A1 (es) |
| IL (1) | IL245313B (es) |
| RU (1) | RU2687369C2 (es) |
| TW (1) | TWI669395B (es) |
| WO (1) | WO2015066637A1 (es) |
| ZA (1) | ZA201601929B (es) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| WO2015070083A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine,Inc. | CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| MX2017002930A (es) | 2014-09-12 | 2017-06-06 | Du Pont | Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso. |
| KR102763527B1 (ko) | 2014-12-03 | 2025-02-05 | 애질런트 테크놀로지스, 인크. | 화학적 변형을 갖는 가이드 rna |
| EP4219730A1 (en) | 2015-01-27 | 2023-08-02 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Method for conducting site-specific modification on entire plant via gene transient expression |
| ES2884838T3 (es) | 2015-04-06 | 2021-12-13 | Univ Leland Stanford Junior | ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS |
| EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
| JP2018527920A (ja) | 2015-08-14 | 2018-09-27 | インスティテュート・オブ・ジェネティクス・アンド・ディヴェロプメンタル・バイオロジー、チャイニーズ・アカデミー・オブ・サイエンシズＩｎｓｔｉｔｕｔｅ ｏｆ Ｇｅｎｅｔｉｃｓ ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔａｌ Ｂｉｏｌｏｇｙ， Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ | 部位特異的ヌクレオチド置換によりグリホサート耐性イネを取得するための方法 |
| US11171126B2 (en) * | 2015-09-04 | 2021-11-09 | Octavo Systems Llc | Configurable substrate and systems |
| US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US11306308B2 (en) | 2015-11-13 | 2022-04-19 | Massachusetts Institute Of Technology | High-throughput CRISPR-based library screening |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| CN106591293A (zh) * | 2016-12-28 | 2017-04-26 | 贵州省草业研究所 | 基于酶切连接从未知基因组中分离已知序列侧翼序列的方法 |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| BR112019019655A2 (pt) | 2017-03-23 | 2020-04-22 | Harvard College | editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| CN107545153B (zh) * | 2017-10-25 | 2021-06-11 | 桂林电子科技大学 | 一种基于卷积神经网络的核小体分类预测方法 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| ES3023563T3 (en) | 2018-10-16 | 2025-06-02 | Blueallele Corp | Methods for targeted insertion of dna in genes |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| CN114127285B (zh) | 2019-03-19 | 2024-09-10 | 布罗德研究所股份有限公司 | 编辑核苷酸序列的方法和组合物 |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| CN114317588B (zh) * | 2020-09-29 | 2023-06-27 | 中国农业科学院生物技术研究所 | 一种作物高温响应型基因组精准甲基化调控回路 |
| US11884915B2 (en) | 2021-09-10 | 2024-01-30 | Agilent Technologies, Inc. | Guide RNAs with chemical modification for prime editing |
| US20230266293A1 (en) * | 2021-09-21 | 2023-08-24 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| CN114369677A (zh) * | 2022-01-04 | 2022-04-19 | 江汉大学 | 一种检测小麦转基因成分和转基因品系的引物组合、试剂盒、检测方法及应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002059294A1 (en) * | 2001-01-26 | 2002-08-01 | Commonwealth Scientific And Industrial Research O Rganisation | Methods and means for producing efficient silencing construct using recombinational cloning |
| EP1504092B2 (en) * | 2002-03-21 | 2014-06-25 | Sangamo BioSciences, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| CA2660485C (en) * | 2006-08-11 | 2016-04-12 | Sangamo Biosciences, Inc. | Zinc finger nuclease-mediated homologous recombination |
| CA2689345C (en) * | 2007-06-05 | 2017-07-11 | Bayer Bioscience N.V. | Methods and means for exact replacement of target dna in eukaryotic organisms |
| WO2010002984A1 (en) * | 2008-07-01 | 2010-01-07 | Monsanto Technology, Llc | Recombinant dna constructs and methods for modulating expression of a target gene |
| CA2747124C (en) * | 2008-12-17 | 2018-09-04 | Dow Agrosciences Llc | Targeted integration into the zp15 locus |
| BRPI1006273A2 (pt) * | 2009-03-20 | 2019-09-24 | Basf Plant Science Gmbh | " vetor de expressão isolado, vegetal transgênico, método para produção de um vegetal transgênico e método para conferir resistência contra nematoide a um vegetal " |
| AU2011207387B2 (en) * | 2010-01-22 | 2015-02-26 | Corteva Agriscience Llc | Excision of transgenes in genetically modified organisms |
| MX2012008486A (es) * | 2010-01-22 | 2012-08-17 | Dow Agrosciences Llc | Plataformas de aterrizaje diseñadas para el envio al objetivo del gen en las plantas. |
| AU2011264074B2 (en) | 2010-06-09 | 2015-01-22 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
| EP2729567B1 (en) * | 2011-07-08 | 2016-10-05 | Cellectis | Method for increasing the efficiency of double-strand break-induced mutagenssis |
| CA2922823C (en) * | 2013-09-04 | 2023-01-17 | Dow Agrosciences Llc | Rapid targeting analysis in crops for determining donor insertion |
-
2014
- 2014-11-03 CN CN201480060417.5A patent/CN105683357B/zh not_active Expired - Fee Related
- 2014-11-03 CA CA2926534A patent/CA2926534A1/en not_active Abandoned
- 2014-11-03 RU RU2016122038A patent/RU2687369C2/ru active
- 2014-11-03 AU AU2014341928A patent/AU2014341928B2/en not_active Ceased
- 2014-11-03 JP JP2016526812A patent/JP6560203B2/ja not_active Expired - Fee Related
- 2014-11-03 TW TW103138081A patent/TWI669395B/zh not_active IP Right Cessation
- 2014-11-03 WO PCT/US2014/063732 patent/WO2015066637A1/en not_active Ceased
- 2014-11-03 KR KR1020167014602A patent/KR102248730B1/ko not_active Expired - Fee Related
- 2014-11-03 BR BR102014027448A patent/BR102014027448A8/pt not_active Application Discontinuation
- 2014-11-03 EP EP14858498.0A patent/EP3066192B1/en not_active Not-in-force
- 2014-11-03 AR ARP140104120A patent/AR098283A1/es unknown
- 2014-11-03 US US14/531,710 patent/US10077449B2/en not_active Expired - Fee Related
-
2016
- 2016-03-18 ZA ZA2016/01929A patent/ZA201601929B/en unknown
- 2016-04-25 IL IL245313A patent/IL245313B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| JP6560203B2 (ja) | 2019-08-14 |
| TW201522630A (zh) | 2015-06-16 |
| RU2687369C2 (ru) | 2019-05-13 |
| CN105683357B (zh) | 2020-10-30 |
| CA2926534A1 (en) | 2015-05-07 |
| KR20160079090A (ko) | 2016-07-05 |
| EP3066192A1 (en) | 2016-09-14 |
| WO2015066637A1 (en) | 2015-05-07 |
| IL245313A0 (en) | 2016-06-30 |
| RU2016122038A3 (es) | 2018-06-21 |
| EP3066192B1 (en) | 2019-05-01 |
| BR102014027448A2 (pt) | 2015-09-15 |
| AU2014341928B2 (en) | 2017-11-30 |
| ZA201601929B (en) | 2019-06-26 |
| US20150143588A1 (en) | 2015-05-21 |
| US10077449B2 (en) | 2018-09-18 |
| JP2016534727A (ja) | 2016-11-10 |
| RU2016122038A (ru) | 2017-12-11 |
| EP3066192A4 (en) | 2017-07-19 |
| KR102248730B1 (ko) | 2021-05-07 |
| IL245313B (en) | 2019-05-30 |
| CN105683357A (zh) | 2016-06-15 |
| BR102014027448A8 (pt) | 2021-08-24 |
| TWI669395B (zh) | 2019-08-21 |
| AU2014341928A1 (en) | 2016-04-07 |
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