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AR097805A1 - Células de cambio de temperatura para la expresión de alto rendimiento de polipéptidos en levadura y otros transformados - Google Patents

Células de cambio de temperatura para la expresión de alto rendimiento de polipéptidos en levadura y otros transformados

Info

Publication number
AR097805A1
AR097805A1 ARP140101246A ARP140101246A AR097805A1 AR 097805 A1 AR097805 A1 AR 097805A1 AR P140101246 A ARP140101246 A AR P140101246A AR P140101246 A ARP140101246 A AR P140101246A AR 097805 A1 AR097805 A1 AR 097805A1
Authority
AR
Argentina
Prior art keywords
temperature
expression
transformed
high performance
temperature change
Prior art date
Application number
ARP140101246A
Other languages
English (en)
Inventor
Hartner Franz
LESNICKI Gary
Dianne Mneill Patricia
Young Mark
Original Assignee
Alder Biopharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alder Biopharmaceuticals Inc filed Critical Alder Biopharmaceuticals Inc
Publication of AR097805A1 publication Critical patent/AR097805A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Métodos para la producción de proteínas heterólogas. En particular, la actual solicitud proporciona métodos de producir proteínas deseadas, incluyendo proteínas múltiples subunidades tales como anticuerpos, con un modificado rendimiento y pureza. En encarnaciones ejemplares, las células transformadas son una levadura, por ejemplo, la levadura metilotrófica como Pichia pastoris. Reivindicación 1: Un método para producir una proteína deseada, que comprende: (a) cultivar células eucarióticas que comprenden uno o más genes que proveen para la expresión de dicha proteína deseada a una primera temperatura; y (b) cultivar dichas células eucarióticas a una segunda temperatura y permitir que dichas células eucarióticas produzcan dicha proteína deseada; en donde dicha segunda temperatura es diferente de dicha primera temperatura.
ARP140101246A 2013-03-15 2014-03-17 Células de cambio de temperatura para la expresión de alto rendimiento de polipéptidos en levadura y otros transformados AR097805A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361790613P 2013-03-15 2013-03-15

Publications (1)

Publication Number Publication Date
AR097805A1 true AR097805A1 (es) 2016-04-20

Family

ID=51538487

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140101246A AR097805A1 (es) 2013-03-15 2014-03-17 Células de cambio de temperatura para la expresión de alto rendimiento de polipéptidos en levadura y otros transformados

Country Status (3)

Country Link
US (2) US10189892B2 (es)
AR (1) AR097805A1 (es)
WO (1) WO2014145521A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2905180C (en) 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US10202630B2 (en) 2013-03-15 2019-02-12 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
AU2016271628B2 (en) 2015-05-29 2020-01-30 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom
EP4063513A1 (en) 2015-05-29 2022-09-28 Cargill, Incorporated Heat treatment to produce glycosides
CN107614677A (zh) * 2015-05-29 2018-01-19 嘉吉公司 用于使用多阶段进料生产甜菊醇糖苷的发酵方法
AU2016301190A1 (en) 2015-08-06 2018-02-15 Cargill, Incorporated Fermentation methods for producing steviol glycosides
CN108220175B (zh) * 2016-12-12 2021-06-18 安琪酵母股份有限公司 酿酒酵母高密度培养方法及其pH调控方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278053A (en) * 1991-09-16 1994-01-11 Eli Lilly And Company Method of producing a polyether antibiotic from actinomadura fibrosa sp. nov. NRRL 18348 and actinomadura sp. NRRL 18880
US6220076B1 (en) * 1999-05-06 2001-04-24 Queen's University At Kingston Differential gas analyzer
US7751981B2 (en) * 2001-10-26 2010-07-06 The Regents Of The University Of California Articles of manufacture and methods for modeling Saccharomyces cerevisiae metabolism
EP1660661A2 (en) 2003-08-08 2006-05-31 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
ITMI20041494A1 (it) 2004-07-23 2004-10-23 Getters Spa Composizioni per il rilascio di mercurio e processo per la loro produzione
JP2006288201A (ja) * 2005-04-04 2006-10-26 Asahi Breweries Ltd 微生物の流加培養方法及び流加培養装置
US8647840B2 (en) * 2007-12-11 2014-02-11 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
WO2010053636A1 (en) * 2008-11-06 2010-05-14 Tate & Lyle Technology Ltd Enhanced production and purification of a natural high intensity sweetener
CA2813513A1 (en) * 2010-10-13 2012-04-19 Dsm Ip Assets B.V. Polypeptides with permease activity

Also Published As

Publication number Publication date
WO2014145521A3 (en) 2014-12-24
US10189892B2 (en) 2019-01-29
US11597758B2 (en) 2023-03-07
US20160031970A1 (en) 2016-02-04
WO2014145521A2 (en) 2014-09-18
US20190211080A1 (en) 2019-07-11

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