AR083569A1 - Metodo para la produccion masiva del factor vii/viia - Google Patents
Metodo para la produccion masiva del factor vii/viiaInfo
- Publication number
- AR083569A1 AR083569A1 ARP110103967A ARP110103967A AR083569A1 AR 083569 A1 AR083569 A1 AR 083569A1 AR P110103967 A ARP110103967 A AR P110103967A AR P110103967 A ARP110103967 A AR P110103967A AR 083569 A1 AR083569 A1 AR 083569A1
- Authority
- AR
- Argentina
- Prior art keywords
- factor vii
- coagulation factor
- human coagulation
- cell line
- mass production
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 229940012413 factor vii Drugs 0.000 title 1
- 229940012414 factor viia Drugs 0.000 title 1
- 101001049020 Homo sapiens Coagulation factor VII Proteins 0.000 abstract 6
- 108010022394 Threonine synthase Proteins 0.000 abstract 4
- 102000004419 dihydrofolate reductase Human genes 0.000 abstract 4
- 210000004102 animal cell Anatomy 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 241000701022 Cytomegalovirus Species 0.000 abstract 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 229940117937 Dihydrofolate reductase inhibitor Drugs 0.000 abstract 1
- 208000031220 Hemophilia Diseases 0.000 abstract 1
- 208000009292 Hemophilia A Diseases 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000003166 dihydrofolate reductase inhibitor Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- MFBOGIVSZKQAPD-UHFFFAOYSA-M sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6437—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para la producción masiva del factor de coagulación VII humano. El método comprende a) construir un vector de expresión que contiene i) un promotor de la dihidrofolato reductasa desprovista de una de más de las secuencias de repetición CCGCC de la región rica en GC del mismo y un gen de dihidrofolato reductasa (DHFR) ligado operativamente al mismo, y ii) un promotor de citomegalovirus (CMV) y un gen del factor de coagulación VII humano ligado operativamente al mismo; b) transfectar el vector de expresión del paso a) en una línea celular animal; c) cultivar la línea celular animal transfectada del paso b) en la presencia de un inhibidor dihidrofolato reductasa para seleccionar células que expresan al factor de coagulación VII humano con gran eficacia; y d) agregar butirato de sodio a las células animales del paso c), y una línea celular para la producción masiva del factor de coagulación VII humano. El uso del vector que contiene un promotor DHFR desprovisto de las secuencias de repetición ricas en GC en el método permite producir al factor de coagulación VII humano con una gran eficacia a gran escala, que contribuye en el tratamiento de la hemofilia. Una línea celular HMF 708 para producir factor de coagulación humano VII.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20100104403 | 2010-10-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR083569A1 true AR083569A1 (es) | 2013-03-06 |
Family
ID=45994561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110103967A AR083569A1 (es) | 2010-10-26 | 2011-10-26 | Metodo para la produccion masiva del factor vii/viia |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9243237B2 (es) |
| EP (1) | EP2633058B1 (es) |
| JP (2) | JP6088974B2 (es) |
| KR (1) | KR101380728B1 (es) |
| AR (1) | AR083569A1 (es) |
| ES (1) | ES2597966T3 (es) |
| TW (1) | TWI541252B (es) |
| WO (1) | WO2012057527A2 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9980471B2 (en) | 2012-06-12 | 2018-05-29 | The General Hospital Corporation | Miniature swine transgenic for one or more coagulation factors |
| KR20150026024A (ko) * | 2013-08-30 | 2015-03-11 | 한미약품 주식회사 | 인간 혈액응고 7인자 유도체의 대량 생산 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR860984B (en) * | 1985-04-17 | 1986-08-18 | Zymogenetics Inc | Expression of factor vii and ix activities in mammalian cells |
| JP2602164B2 (ja) | 1993-05-31 | 1997-04-23 | デコンタインテグレ株式会社 | 有機廃水処理方法及びその方法で使用する有機廃水処理装置 |
| KR0162021B1 (ko) * | 1995-07-27 | 1998-11-16 | 김은영 | 에리트로포이에틴의 제조방법 |
| KR100397791B1 (ko) * | 2000-08-30 | 2003-09-13 | 한국과학기술원 | ＣＨＯ세포에서 ｂｃｌ-2 유전자 과발현 및 배지내 소디움부티레이트 첨가에 의한 인간화된 항체의 생산성을증가시키는 방법 |
| US7176288B2 (en) | 2000-09-13 | 2007-02-13 | Novo Nordisk Healthcare A/G | Human coagulation factor VII variants |
| US20040185535A1 (en) | 2003-03-21 | 2004-09-23 | Giles Wilson | Industrial-scale serum-free production of recombinant FVII in mammalian cells |
| KR100880509B1 (ko) * | 2006-10-16 | 2009-01-28 | 한미약품 주식회사 | 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법 |
| KR101030978B1 (ko) * | 2008-07-10 | 2011-04-28 | (주) 에이프로젠 | 동물세포용 재조합 발현벡터 |
-
2011
- 2011-10-26 JP JP2013536513A patent/JP6088974B2/ja not_active Expired - Fee Related
- 2011-10-26 EP EP11836619.4A patent/EP2633058B1/en not_active Not-in-force
- 2011-10-26 US US13/881,974 patent/US9243237B2/en not_active Expired - Fee Related
- 2011-10-26 KR KR1020110109882A patent/KR101380728B1/ko not_active Expired - Fee Related
- 2011-10-26 ES ES11836619.4T patent/ES2597966T3/es active Active
- 2011-10-26 AR ARP110103967A patent/AR083569A1/es unknown
- 2011-10-26 WO PCT/KR2011/008040 patent/WO2012057527A2/en not_active Ceased
- 2011-10-26 TW TW100138824A patent/TWI541252B/zh not_active IP Right Cessation
-
2016
- 2016-09-30 JP JP2016192657A patent/JP2017042168A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2633058B1 (en) | 2016-07-20 |
| TWI541252B (zh) | 2016-07-11 |
| JP6088974B2 (ja) | 2017-03-01 |
| WO2012057527A3 (en) | 2012-07-26 |
| EP2633058A2 (en) | 2013-09-04 |
| US9243237B2 (en) | 2016-01-26 |
| KR20120043657A (ko) | 2012-05-04 |
| EP2633058A4 (en) | 2014-04-16 |
| TW201307382A (zh) | 2013-02-16 |
| US20130273634A1 (en) | 2013-10-17 |
| ES2597966T3 (es) | 2017-01-24 |
| WO2012057527A2 (en) | 2012-05-03 |
| KR101380728B1 (ko) | 2014-04-03 |
| JP2013544080A (ja) | 2013-12-12 |
| JP2017042168A (ja) | 2017-03-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |