MX2018003354A - Seleccion de celulas pluripotentes para producir ratones hembra xy fertiles. - Google Patents
Seleccion de celulas pluripotentes para producir ratones hembra xy fertiles.Info
- Publication number
- MX2018003354A MX2018003354A MX2018003354A MX2018003354A MX2018003354A MX 2018003354 A MX2018003354 A MX 2018003354A MX 2018003354 A MX2018003354 A MX 2018003354A MX 2018003354 A MX2018003354 A MX 2018003354A MX 2018003354 A MX2018003354 A MX 2018003354A
- Authority
- MX
- Mexico
- Prior art keywords
- animal
- compositions
- methods
- cells
- pluripotent
- Prior art date
Links
- 241000699670 Mus sp. Species 0.000 title 1
- 241001465754 Metazoa Species 0.000 abstract 5
- 230000001135 feminizing effect Effects 0.000 abstract 5
- 238000000034 method Methods 0.000 abstract 5
- 239000000203 mixture Substances 0.000 abstract 5
- 210000004102 animal cell Anatomy 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 3
- 210000002257 embryonic structure Anatomy 0.000 abstract 3
- 210000002593 Y chromosome Anatomy 0.000 abstract 2
- 238000012606 in vitro cell culture Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000030279 gene silencing Effects 0.000 abstract 1
- 230000004952 protein activity Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5073—Stem cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/12—Animals modified by administration of exogenous cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/35—Animals modified by environmental factors, e.g. temperature, O2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Environmental Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se proporcionan métodos y composiciones para generar animales hembra XY fértiles FO. Los métodos y composiciones implican 5 producir células animales pluripotentes o totipotentes XY, cultivos celulares in vitro o embriones que sean capaces de producir un animal XY hembra fértil en una generación FO. Dichas células, embriones y animales pueden elaborarse al silenciar una región del cromosoma Y. De manera opcional, las 10 células también pueden cultivarse en medio feminizante, tal como un medio con osmolalidad baja, y/o pueden modificarse para disminuir el nivel y/o actividad de una proteína Sry. También se proporcionan métodos y composiciones para silenciar una región del cromosoma Y en una célula animal pluripotente o 15 totipotente XY, o cultivos celulares in vitro, embriones o animales derivados de estos, al mantener la célula animal pluripotente o totipotente XY en un medio feminizante. También se proporcionan métodos y composiciones para mantener una población de células animales pluripotentes o totipotentes XY 20 en un medio feminizante y seleccionar células o clones que tengan capacidades aumentadas para producir un animal XY hembra fértil en una generación FO. También se proporcionan métodos y composiciones para evaluar los compuestos con actividad feminizante o para optimizar las concentraciones de componentes 25 en el medio feminizante.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562219927P | 2015-09-17 | 2015-09-17 | |
| PCT/US2016/052345 WO2017049240A1 (en) | 2015-09-17 | 2016-09-16 | Selection of pluripotent cells for production of fertile xy female mice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2018003354A true MX2018003354A (es) | 2018-05-28 |
| MX388475B MX388475B (es) | 2025-03-20 |
Family
ID=57113699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018003354A MX388475B (es) | 2015-09-17 | 2016-09-16 | Selección de células pluripotentes para producir ratones hembra xy fértiles. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10893666B2 (es) |
| EP (1) | EP3349573A1 (es) |
| JP (1) | JP6868615B2 (es) |
| KR (1) | KR102382458B1 (es) |
| CN (1) | CN108697068B (es) |
| AU (1) | AU2016324305B2 (es) |
| CA (1) | CA2998642C (es) |
| HK (1) | HK1256687A1 (es) |
| IL (1) | IL257982B2 (es) |
| MX (1) | MX388475B (es) |
| RU (1) | RU2729407C1 (es) |
| SG (1) | SG10201913789VA (es) |
| WO (1) | WO2017049240A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2016324305B2 (en) | 2015-09-17 | 2022-03-03 | Regeneron Pharmaceuticals, Inc. | Selection of pluripotent cells for production of fertile XY female mice |
| CN108998540A (zh) * | 2018-08-18 | 2018-12-14 | 兰州大学 | 绵羊eif2s3y基因的单核苷酸多态性标记及其检测引物、试剂盒、方法及应用 |
| CN110656167A (zh) * | 2019-09-27 | 2020-01-07 | 中国科学院苏州生物医学工程技术研究所 | 用于y染色体微缺失检测的引物组、试剂盒及基于数字pcr的检测方法 |
| CN111321232B (zh) * | 2020-03-18 | 2022-08-16 | 西北农林科技大学 | 一种快速检测肉牛eif4a2基因拷贝数变异的方法及其应用 |
| CN113558012A (zh) * | 2021-08-05 | 2021-10-29 | 陈米米 | 一种哺乳动物繁育雌性多胎的方法 |
| CN114015705A (zh) * | 2021-11-28 | 2022-02-08 | 华中科技大学同济医学院附属协和医院 | 一种小鼠体外受精繁育性别选择方法 |
| CN115251007B (zh) * | 2022-09-02 | 2024-01-26 | 山西医科大学 | 一种新品系裸大鼠、其培育方法及应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0907722A1 (en) | 1996-04-29 | 1999-04-14 | Leuven Research & Development V.Z.W. | Pluripotent rabbit embryonic stem (es) cell lines and their use in the generation of chimeric rabbits |
| EP1067141A1 (en) | 1999-06-11 | 2001-01-10 | Rijksuniversiteit te Leiden | A male-specific protein homologue involved in histocompatibility |
| EP1227719A4 (en) | 1999-11-02 | 2003-01-15 | Univ Massachusetts | USE OF HAPLOID GENOMES FOR GENETIC DIAGNOSIS, MODIFICATION AND MULTIPLICATION |
| JP2003517317A (ja) | 1999-12-20 | 2003-05-27 | ペリー、アンソニー、シー．エフ． | 培養細胞からクローン化胚及び成体を作製する方法 |
| EP1486116A4 (en) | 2002-02-27 | 2008-01-23 | Yasumitsu Nagao | METHOD FOR CONSTRUCTING CHEMICAL ANIMAL WITH GERMINAL LINE |
| DE60301953T2 (de) | 2002-03-05 | 2006-07-27 | Artemis Pharmaceuticals Gmbh | Durch inzucht erzeugte von embryonalen stammzellen abgeleitete mäuse |
| EP1516924A1 (en) | 2003-09-17 | 2005-03-23 | Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) | Generation of human embryonic stem cells from triploid zygotes |
| KR101178786B1 (ko) | 2004-03-23 | 2012-09-07 | 다이이찌 산쿄 가부시키가이샤 | 다능성 줄기세포의 증식 방법 |
| PL2767161T3 (pl) | 2004-10-19 | 2018-09-28 | Regeneron Pharmaceuticals, Inc. | Sposób wytwarzania zwierzęcia homozygotycznego pod względem modyfikacji genetycznej |
| EP2500417A1 (en) | 2006-08-09 | 2012-09-19 | Vivalis | Method of production of transgenic avian using embryonic stem cells |
| GB0623635D0 (en) | 2006-11-27 | 2007-01-03 | Stem Cell Sciences Uk Ltd | Pluripotent cell growth media |
| WO2009037337A1 (en) * | 2007-09-20 | 2009-03-26 | Novartis Ag | Robust and tissue independent gender-specific transcript markers for molecular gender determination |
| BRPI1008197A2 (pt) | 2009-02-08 | 2016-05-03 | Murdoch Childrens Res Inst | determinação de sexo e métodos de especificação do mesmo. |
| EP2488631B1 (en) | 2009-10-13 | 2020-01-08 | Stemcell Technologies Inc. | Manipulation of osmolality for differentiating stem cells |
| ES2727738T3 (es) | 2010-06-11 | 2019-10-18 | Regeneron Pharma | Producción de animales hembra xy fértiles a partir de células es xy |
| WO2012022634A1 (en) | 2010-08-16 | 2012-02-23 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Classification, diagnosis and prognosis of multiple myeloma |
| ES2694629T3 (es) | 2014-06-26 | 2018-12-26 | Regeneron Pharmaceuticals, Inc. | Métodos y composiciones para modificaciones genéticas objetivo y métodos de uso |
| CN107002042A (zh) | 2014-10-15 | 2017-08-01 | 瑞泽恩制药公司 | 用于产生或维持多能细胞的方法和组合物 |
| AU2016324305B2 (en) | 2015-09-17 | 2022-03-03 | Regeneron Pharmaceuticals, Inc. | Selection of pluripotent cells for production of fertile XY female mice |
-
2016
- 2016-09-16 AU AU2016324305A patent/AU2016324305B2/en active Active
- 2016-09-16 SG SG10201913789VA patent/SG10201913789VA/en unknown
- 2016-09-16 CN CN201680067024.6A patent/CN108697068B/zh active Active
- 2016-09-16 MX MX2018003354A patent/MX388475B/es unknown
- 2016-09-16 WO PCT/US2016/052345 patent/WO2017049240A1/en not_active Ceased
- 2016-09-16 EP EP16778528.6A patent/EP3349573A1/en active Pending
- 2016-09-16 KR KR1020187009166A patent/KR102382458B1/ko active Active
- 2016-09-16 JP JP2018514376A patent/JP6868615B2/ja active Active
- 2016-09-16 US US15/268,452 patent/US10893666B2/en active Active
- 2016-09-16 RU RU2018113330A patent/RU2729407C1/ru active
- 2016-09-16 HK HK18115672.3A patent/HK1256687A1/zh unknown
- 2016-09-16 CA CA2998642A patent/CA2998642C/en active Active
-
2018
- 2018-03-08 IL IL257982A patent/IL257982B2/en unknown
-
2020
- 2020-12-11 US US17/119,076 patent/US12041918B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR102382458B1 (ko) | 2022-04-04 |
| WO2017049240A8 (en) | 2018-05-24 |
| AU2016324305A9 (en) | 2019-01-03 |
| IL257982B1 (en) | 2023-01-01 |
| EP3349573A1 (en) | 2018-07-25 |
| JP2018527005A (ja) | 2018-09-20 |
| NZ740899A (en) | 2021-11-26 |
| SG10201913789VA (en) | 2020-03-30 |
| US20170079250A1 (en) | 2017-03-23 |
| MX388475B (es) | 2025-03-20 |
| AU2016324305A1 (en) | 2018-04-12 |
| CN108697068B (zh) | 2022-02-11 |
| KR20180058731A (ko) | 2018-06-01 |
| CA2998642C (en) | 2023-09-12 |
| WO2017049240A1 (en) | 2017-03-23 |
| RU2729407C1 (ru) | 2020-08-06 |
| HK1256687A1 (zh) | 2019-10-04 |
| CA2998642A1 (en) | 2017-03-23 |
| US10893666B2 (en) | 2021-01-19 |
| IL257982A (en) | 2018-05-31 |
| CN108697068A (zh) | 2018-10-23 |
| US20210112788A1 (en) | 2021-04-22 |
| US12041918B2 (en) | 2024-07-23 |
| IL257982B2 (en) | 2023-05-01 |
| JP6868615B2 (ja) | 2021-05-12 |
| AU2016324305B2 (en) | 2022-03-03 |
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