AR077844A1 - Metodos, cebadores, sondas y conjuntos de elementos para detectar mutaciones braf - Google Patents
Metodos, cebadores, sondas y conjuntos de elementos para detectar mutaciones brafInfo
- Publication number
- AR077844A1 AR077844A1 ARP100102935A ARP100102935A AR077844A1 AR 077844 A1 AR077844 A1 AR 077844A1 AR P100102935 A ARP100102935 A AR P100102935A AR P100102935 A ARP100102935 A AR P100102935A AR 077844 A1 AR077844 A1 AR 077844A1
- Authority
- AR
- Argentina
- Prior art keywords
- braf
- seq
- sample
- sequence
- mutant
- Prior art date
Links
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 title abstract 12
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 title abstract 12
- 239000000523 sample Substances 0.000 title abstract 9
- 230000035772 mutation Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000003321 amplification Effects 0.000 abstract 7
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 7
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 6
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 102200055464 rs113488022 Human genes 0.000 abstract 1
- 102220197820 rs121913227 Human genes 0.000 abstract 1
- 102200055469 rs121913377 Human genes 0.000 abstract 1
- 102220014069 rs121913378 Human genes 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Paints Or Removers (AREA)
Abstract
Métodos, cebadores y sondas de utilidad para detectar la presencia de secuencias BRAF mutantes en una muestra, específicamente para detectar la presencia de las mutaciones BRAF V600E, V600D, V600K y V600M, por lo que es posible usarlas para diagnosticar diversos canceres. Reivindicacion 1: Un método para detectar la presencia de una mutacion BRAF en una muestra, caracterizado porque comprende (a) aislar un ácido nucleico de dicha muestra, donde la muestra comprende secuencias de ADN; (b) realizar una reaccion de amplificacion con las secuencias de ADN de dicha muestra, donde dicha reaccion de amplificacion comprende un primer cebador capaz de alinearse específicamente con una secuencia BRAF mutante en una primera posicion en una secuencia de AUN BRAF, donde dicho primer cebador es SEQ ID Ns 1, SEQ ID Ns 2, SEQ ID Ns 3, SEQ ID Ns 4, SEQ ID Ns 5, SEQ ID Ns 6, SEQ ID Ns 7, SEQ ID Ns 8 o SEQ ID Ns 9, y un segundo cebador capaz de alinearse específicamente en una segunda posicion en una secuencia de ADN BRAF, donde dichos cebadores, el primero y el segundo, se alinean con las distintas cadenas de la secuencia de ADN BRAF de cadena doble, donde la reaccion de amplificacion permite generar un producto de amplificacion específico para BRAF mutante cuando las secuencias de ADN de la muestra comprenden una secuencia de ADN BRAF que comprende una secuencia mutante en dicha primera posicion de dicha secuencia de AUN BRAF; y (c) visualizar los productos de amplificacion generados en dicha reaccion de amplificacion, donde la deteccion de un producto de amplificacion especifico para BRAF mutante es un indicador positivo de una mutacion BRAF en dicha muestra.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23305409P | 2009-08-11 | 2009-08-11 | |
| US23707809P | 2009-08-26 | 2009-08-26 | |
| US30179010P | 2010-02-05 | 2010-02-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR077844A1 true AR077844A1 (es) | 2011-09-28 |
Family
ID=43586772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP100102935A AR077844A1 (es) | 2009-08-11 | 2010-08-11 | Metodos, cebadores, sondas y conjuntos de elementos para detectar mutaciones braf |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20140248614A1 (es) |
| EP (1) | EP2464751B1 (es) |
| JP (1) | JP2013501523A (es) |
| KR (1) | KR20120099630A (es) |
| CN (1) | CN102575295B (es) |
| AR (1) | AR077844A1 (es) |
| AU (1) | AU2010282632A1 (es) |
| CA (1) | CA2770716A1 (es) |
| IL (1) | IL218021A0 (es) |
| NZ (1) | NZ598166A (es) |
| TW (1) | TW201111517A (es) |
| WO (1) | WO2011019704A2 (es) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6153758B2 (ja) * | 2012-04-20 | 2017-06-28 | アークレイ株式会社 | 多型検出用プローブ、多型検出方法、薬効判定方法及び多型検出用キット |
| US10077474B2 (en) | 2012-05-29 | 2018-09-18 | Abbott Molecular, Inc. | Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits |
| CN102816851A (zh) * | 2012-08-29 | 2012-12-12 | 苏州旷远生物分子技术有限公司 | 用于检测braf基因v600e突变的引物、探针、试剂盒及方法 |
| CN102876784B (zh) * | 2012-09-13 | 2014-11-19 | 周宏灏 | 焦磷酸测序法检测B-raf基因多态性的试剂盒及方法 |
| EP2711432A1 (en) * | 2012-09-21 | 2014-03-26 | Genomica S.A.U. | Method for detection of BRAF and PI3K mutations |
| CA2895828C (en) * | 2012-12-27 | 2020-06-02 | Quest Diagnostics Investments Incorporated | Ddr2 mutations as targetable features of melanoma or basal cell carcinoma |
| CN103103269B (zh) * | 2013-01-18 | 2015-07-15 | 陕西佰美基因股份有限公司 | 一种基于探针熔解技术准确检测braf基因突变的方法 |
| EP2971147A4 (en) * | 2013-03-15 | 2016-08-31 | Abbott Molecular Inc | MULTIPLEX ALLELNACHWEIS |
| KR101378920B1 (ko) * | 2013-04-18 | 2014-03-27 | 주식회사 현일바이오 | Ｂｒａｆ 돌연변이의 선택적 검출 방법 및 이를 이용한 키트 |
| CN105705655A (zh) * | 2013-08-14 | 2016-06-22 | 佳根曼斯菲尔德有限公司 | 用于对nras和braf核酸进行多重分析的组合物及方法 |
| CN104017887A (zh) * | 2014-06-18 | 2014-09-03 | 广州好芝生物科技有限公司 | 人类braf基因突变检测引物对和探针及其试剂盒 |
| AU2015287550A1 (en) * | 2014-07-11 | 2017-01-12 | Expression Pathology, Inc. | SRM/MRM assay for the Serine/Threonine-Protein Kinase B-raf (BRAF) |
| CN105400900A (zh) * | 2015-12-29 | 2016-03-16 | 杭州迪安生物技术有限公司 | 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用 |
| CN105861691A (zh) * | 2016-05-06 | 2016-08-17 | 北京晋祺生物科技有限公司 | 一种braf基因突变检测的引物组合物、试剂盒及方法 |
| US12364776B2 (en) | 2017-06-29 | 2025-07-22 | Korea Advanced Institute Of Science And Technology | Ganglioglioma-induced animal model and a method for diagnosing and treating ganglioglioma and related diseases |
| KR20190038464A (ko) * | 2017-09-29 | 2019-04-08 | (재)록원바이오융합연구재단 | Ras/braf 돌연변이 검출용 조성물 및 이를 포함하는 키트 |
| CN109161594A (zh) * | 2018-08-17 | 2019-01-08 | 中山大学达安基因股份有限公司 | 一种检测braf基因突变的方法及其试剂盒 |
| WO2022119423A1 (ko) * | 2020-12-04 | 2022-06-09 | 주식회사 제노픽스 | 폐암 유전자 돌연변이 초고감도 선택적 증폭 방법 및 이를 위한 조성물 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004337120A (ja) * | 2003-05-19 | 2004-12-02 | Chang Gung Univ | 膀胱癌再発検査方法 |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| CN101487051B (zh) * | 2009-02-24 | 2011-07-20 | 广州益善生物技术有限公司 | Braf基因突变的检测探针、液相芯片及其检测方法 |
-
2010
- 2010-08-10 EP EP10808619.0A patent/EP2464751B1/en not_active Not-in-force
- 2010-08-10 JP JP2012524782A patent/JP2013501523A/ja active Pending
- 2010-08-10 CN CN201080045765.7A patent/CN102575295B/zh not_active Expired - Fee Related
- 2010-08-10 CA CA2770716A patent/CA2770716A1/en not_active Abandoned
- 2010-08-10 AU AU2010282632A patent/AU2010282632A1/en not_active Abandoned
- 2010-08-10 KR KR1020127004699A patent/KR20120099630A/ko not_active Ceased
- 2010-08-10 NZ NZ598166A patent/NZ598166A/en not_active IP Right Cessation
- 2010-08-10 WO PCT/US2010/044997 patent/WO2011019704A2/en not_active Ceased
- 2010-08-11 AR ARP100102935A patent/AR077844A1/es unknown
- 2010-08-11 TW TW099126740A patent/TW201111517A/zh unknown
-
2012
- 2012-02-09 IL IL218021A patent/IL218021A0/en unknown
-
2014
- 2014-02-27 US US14/192,414 patent/US20140248614A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| IL218021A0 (en) | 2012-04-30 |
| AU2010282632A1 (en) | 2012-03-08 |
| CA2770716A1 (en) | 2011-02-17 |
| EP2464751A2 (en) | 2012-06-20 |
| EP2464751B1 (en) | 2017-05-24 |
| US20140248614A1 (en) | 2014-09-04 |
| WO2011019704A2 (en) | 2011-02-17 |
| CN102575295A (zh) | 2012-07-11 |
| EP2464751A4 (en) | 2013-07-03 |
| WO2011019704A3 (en) | 2011-07-07 |
| CN102575295B (zh) | 2016-02-17 |
| TW201111517A (en) | 2011-04-01 |
| NZ598166A (en) | 2014-03-28 |
| KR20120099630A (ko) | 2012-09-11 |
| HK1172931A1 (zh) | 2013-05-03 |
| JP2013501523A (ja) | 2013-01-17 |
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| Date | Code | Title | Description |
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| FB | Suspension of granting procedure |