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AR077402A2 - Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas - Google Patents

Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas

Info

Publication number
AR077402A2
AR077402A2 ARP100102457A ARP100102457A AR077402A2 AR 077402 A2 AR077402 A2 AR 077402A2 AR P100102457 A ARP100102457 A AR P100102457A AR P100102457 A ARP100102457 A AR P100102457A AR 077402 A2 AR077402 A2 AR 077402A2
Authority
AR
Argentina
Prior art keywords
seq
bases
nucleic acid
plant
regulatory region
Prior art date
Application number
ARP100102457A
Other languages
English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR077402A2 publication Critical patent/AR077402A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Reivindicacion 1: Un método para conmutar la fertilidad masculina de una planta, dicho método caracterizado porque comprende: introducir en el ADN de una planta un vector de expresion de un constructo que comprende una region reguladora heterologa con especificidad para tejidos masculinos de plantas heterologas ligada a un segundo ácido nucleico, donde dicha region reguladora se selecciona del grupo que consiste en: (i) SEQ ID NO:5; (ii) SEQ ID NO:6; (iii) bases 1 a 1088 de SEQ ID NO:7; (iv) bases 830 a 962 de SEQ ID NO:7; (v) bases 830 a 914 de SEQ ID NO:7; (vi) bases 917 a 962 de SEQ ID NO:7; (vii) bases 875 a 954 de SEQ ID NO:7; (viii) bases 935 a 954 de SEQ ID NO:7; (ix) bases 975 a 924 de SEQ ID NO:7; (x) bases 1 a 1005 de SEQ ID NO:7; y (xi) un fragmento de las bases 1 a 1088 de la SEQ ID NO:7; y donde dicha region reguladora esta ligada operativamente al segundo ácido nucleico que es una secuencia que reporta (ôreporterö) o una secuencia nucleotida heterologa, donde dicho segundo ácido nucleico es una secuencia nucleotida heterologa que está ligada operativamente a la region reguladora, de manera que la region reguladora regula la expresion del segundo ácido nucleico en el tejido masculino, y cultivar una planta en la cual el segundo ácido nucleico está incorporado en forma estable dentro del genoma de la planta y expresa el segundo ácido nucleico en el tejido masculino de la planta, donde el segundo ácido nucleico controla la fertilidad masculina de la planta.
ARP100102457A 2000-09-26 2010-07-07 Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas AR077402A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67015300A 2000-09-26 2000-09-26

Publications (1)

Publication Number Publication Date
AR077402A2 true AR077402A2 (es) 2011-08-24

Family

ID=24689201

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP010104519A AR030961A1 (es) 2000-09-26 2001-09-25 Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas
ARP100102457A AR077402A2 (es) 2000-09-26 2010-07-07 Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP010104519A AR030961A1 (es) 2000-09-26 2001-09-25 Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas

Country Status (10)

Country Link
US (4) US7098388B2 (es)
EP (1) EP1320618B1 (es)
AR (2) AR030961A1 (es)
AT (1) ATE548458T1 (es)
AU (3) AU9122801A (es)
BR (1) BR0114199A (es)
CA (2) CA2423480C (es)
HU (1) HU228694B1 (es)
MX (1) MXPA03002572A (es)
WO (1) WO2002026789A2 (es)

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WO2002026789A2 (en) * 2000-09-26 2002-04-04 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
EP2333075B1 (en) 2003-12-16 2018-07-04 Pioneer Hi-Bred International Inc. Dominant gene suppression transgenes and methods of using same
AU2011265403B2 (en) * 2003-12-16 2012-07-05 Pioneer Hi-Bred International, Inc. Dominant gene suppression transgenes and methods of using same
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US7919676B2 (en) 2007-08-03 2011-04-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
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WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
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CN105143248A (zh) 2013-03-14 2015-12-09 先锋国际良种公司 玉蜀黍胁迫相关转录因子18及其用途
CA2903555A1 (en) 2013-03-15 2014-09-18 Pioneer Hi-Bred International, Inc. Compositions and methods of use of acc oxidase polynucleotides and polypeptides
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BR112017000482A2 (pt) 2014-07-11 2017-11-07 Du Pont métodos para produzir uma planta mutante e para gerar uma planta, planta, semente, rna, métodos para produzir uma célula, para duplicar um fragmento gênico, para substituir uma primeira sequência promotora, para inserir um elemento regulador em uma sequência de nucleotídeos e para inserir um íntron em uma sequência de nucleotídeos, planta de milho e célula vegetal
WO2016100309A1 (en) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
WO2016137774A1 (en) 2015-02-25 2016-09-01 Pioneer Hi-Bred International Inc Composition and methods for regulated expression of a guide rna/cas endonuclease complex
WO2016186953A1 (en) 2015-05-15 2016-11-24 Pioneer Hi Bred International Inc Guide rna/cas endonuclease systems
KR20180069832A (ko) 2015-10-20 2018-06-25 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 유도 cas 시스템을 통한 비기능성 유전자 산물에 대한 기능 회복 및 이용 방법
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
BR112018076027A2 (pt) 2016-06-14 2019-03-26 Pioneer Hi-Bred International, Inc. método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
WO2017222773A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
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Also Published As

Publication number Publication date
WO2002026789A9 (en) 2003-07-03
CA2760906C (en) 2016-11-29
US20020083483A1 (en) 2002-06-27
HU228694B1 (en) 2013-05-28
AU2001291228B2 (en) 2007-01-11
AU2007201072A1 (en) 2007-03-29
AU9122801A (en) 2002-04-08
AU2007201072B2 (en) 2009-09-17
ATE548458T1 (de) 2012-03-15
WO2002026789A2 (en) 2002-04-04
AR030961A1 (es) 2003-09-03
US7151205B2 (en) 2006-12-19
CA2423480A1 (en) 2002-04-04
WO2002026789A3 (en) 2003-02-13
AU2007201072C1 (en) 2010-02-11
US7098388B2 (en) 2006-08-29
MXPA03002572A (es) 2004-08-12
US20080134362A1 (en) 2008-06-05
US20060212971A1 (en) 2006-09-21
CA2423480C (en) 2012-02-14
HUP0501107A2 (en) 2006-06-28
EP1320618A2 (en) 2003-06-25
US20030182689A1 (en) 2003-09-25
EP1320618B1 (en) 2012-03-07
BR0114199A (pt) 2006-01-31
CA2760906A1 (en) 2002-04-04

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