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AR052111A1 - Supresiones geneticas dirigidas para polisacaridos formadores de limo - Google Patents

Supresiones geneticas dirigidas para polisacaridos formadores de limo

Info

Publication number
AR052111A1
AR052111A1 ARP060100398A ARP060100398A AR052111A1 AR 052111 A1 AR052111 A1 AR 052111A1 AR P060100398 A ARP060100398 A AR P060100398A AR P060100398 A ARP060100398 A AR P060100398A AR 052111 A1 AR052111 A1 AR 052111A1
Authority
AR
Argentina
Prior art keywords
polysaccharides
diutan
cell
polisacarids
suppressions
Prior art date
Application number
ARP060100398A
Other languages
English (en)
Inventor
Nancy E Harding
Yamini Patel
Original Assignee
Cp Kelco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cp Kelco Us Inc filed Critical Cp Kelco Us Inc
Publication of AR052111A1 publication Critical patent/AR052111A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Las cepas de Sphingomonas tienen polisacáridos extracelulares (por ejemplo, gellan, diutan) que están unidos firmemente a la superficie de la célula. Esta union puede limitar la produccion de polisacáridos, al impedir la asimilacion de nutrientes en la célula, debido a los sitios limitados para la biosíntesis de polisacáridos en la superficie de la célula. Con mutaciones de supresion, se han inactivado dos genes para la biosíntesis de polisacáridos, denominados gelM y gelN en las cepas productoras de gellan, y dpsM y dpsN en la cepas productoras de diutan, y se ha demostrado que producen polisacáridos que no están unidos firmemente a la superficie de la célula, es decir, toman la forma de limo. Se demostro el carácter de limo a través de la capacidad de las células de centrifugarse y la ausencia de agrupamientos, lo que puede observarse bajo el microscopio o en suspensiones diluidas. Las mutantes productoras de limo de diutan presentaron una productividad algo mayor, y el diutan tuvo una reología significativamente mejorada.
ARP060100398A 2005-02-04 2006-02-03 Supresiones geneticas dirigidas para polisacaridos formadores de limo AR052111A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US64955905P 2005-02-04 2005-02-04

Publications (1)

Publication Number Publication Date
AR052111A1 true AR052111A1 (es) 2007-02-28

Family

ID=36953794

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP060100398A AR052111A1 (es) 2005-02-04 2006-02-03 Supresiones geneticas dirigidas para polisacaridos formadores de limo
ARP150104167A AR103092A2 (es) 2005-02-04 2015-12-17 Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP150104167A AR103092A2 (es) 2005-02-04 2015-12-17 Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende

Country Status (5)

Country Link
US (7) US7888333B2 (es)
CN (5) CN101501213B (es)
AR (2) AR052111A1 (es)
TW (1) TWI467015B (es)
WO (1) WO2006096269A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110027838A1 (en) 2009-07-31 2011-02-03 Harding Nancy E Sphingomonas Strains Producing Greatly Increased Yield Of PHB-Deficient Sphingan (Diutan)
US8343896B2 (en) 2005-01-24 2013-01-01 Halliburton Energy Services, Inc. Sealant compositions comprising diutan and associated methods
CN101501213B (zh) * 2005-02-04 2013-07-17 Cp凯尔科美国公司 多糖粘液形成菌的靶基因缺失
DOP2006000195A (es) 2005-09-07 2017-08-15 Amgen Fremont Inc Anticuerpos monoclonales humanos para la quinasa-1 tipo receptor de activina
US7868167B2 (en) 2005-11-01 2011-01-11 Cp Kelco U.S., Inc. High viscosity diutan gums
AU2015200175A1 (en) * 2005-11-01 2015-02-05 Cp Kelco U.S., Inc. High viscosity diutan gums and methods of producing
BRPI0618145B1 (pt) * 2005-11-01 2022-06-07 Cp Kelco U.S., Inc Goma de diutana
KR101372110B1 (ko) * 2005-11-01 2014-03-14 씨피 켈코 유에스, 인코포레이티드 고점도 디우탄 검 및 제조 방법
US7584791B2 (en) 2007-02-08 2009-09-08 Halliburton Energy Services, Inc. Methods for reducing the viscosity of treatment fluids comprising diutan
US7960315B2 (en) * 2007-02-08 2011-06-14 Halliburton Energy Services, Inc. Treatment fluids comprising diutan and associated methods
US9016375B2 (en) 2011-11-30 2015-04-28 Halliburton Energy Services, Inc. Breaking diutan with oxalic acid at 180° F to 220° F
US20130333886A1 (en) 2012-06-19 2013-12-19 Halliburton Energy Services, Inc. Breaking diutan with metal activitor down to 140 °f or lower
CN103305566B (zh) * 2013-06-14 2014-12-10 浙江大学 一种提高细菌多糖产量及粘度的培养方法
CN106977618B (zh) * 2017-04-13 2022-07-01 张星昊 一种从定优胶发酵液中提取定优胶的方法
CN109735556A (zh) * 2019-02-22 2019-05-10 昆明理工大学 引导糖基转移酶基因的用途
CN110144318B (zh) * 2019-04-22 2021-03-26 南京工业大学 无色素低分子量威兰胶生产菌株及其构建方法与应用
FR3113494A1 (fr) * 2020-08-24 2022-02-25 IFP Energies Nouvelles Procédé de production de Welan de haute viscosité parSphingomonas sp .
US11694876B2 (en) 2021-12-08 2023-07-04 Applied Materials, Inc. Apparatus and method for delivering a plurality of waveform signals during plasma processing
CN115725488B (zh) * 2022-09-27 2025-09-30 南京轩凯生物科技股份有限公司 一株无色素产生的骆驼刺泛菌及其在制备胞外多糖中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985623A (en) 1995-01-24 1999-11-16 Shin-Etsu Bio, Inc. DNA segments and methods for increasing polysaccharide production
WO2000058412A1 (en) * 1999-03-31 2000-10-05 Penford Corporation Paper coating composition comprising gellan gum/starch blend
ATE394490T1 (de) * 2000-03-02 2008-05-15 Cp Kelco Us Inc Mutante bakteriestämme vom genus sphingonomas die defizient in der herstellung von polyhydroxybutyrate sind, und verfahren zur klärung von sphinganen
CA2351233C (en) * 2000-07-24 2010-10-19 Shin-Etsu Chemical Co., Ltd. Production of exopolysaccharides unattached to the surface of bacterial cells
US20030100078A1 (en) 2001-07-03 2003-05-29 Harding Nancy E. Mutant strain of Sphingomonas elodea which produces non-acetylated gellan gum
US6620775B2 (en) * 2001-11-26 2003-09-16 Cp Kelco U.S. Inc. Viscosity stabilization in alkaline solutions
CN101501213B (zh) * 2005-02-04 2013-07-17 Cp凯尔科美国公司 多糖粘液形成菌的靶基因缺失

Also Published As

Publication number Publication date
CN101501213B (zh) 2013-07-17
CN103087941B (zh) 2015-09-16
US9845476B2 (en) 2017-12-19
WO2006096269A2 (en) 2006-09-14
CN103087970A (zh) 2013-05-08
US20110171721A1 (en) 2011-07-14
CN103087940A (zh) 2013-05-08
CN101501213A (zh) 2009-08-05
CN103087970B (zh) 2016-12-21
US8759071B2 (en) 2014-06-24
US20060199201A1 (en) 2006-09-07
CN103146034A (zh) 2013-06-12
AR103092A2 (es) 2017-04-12
US20140342436A1 (en) 2014-11-20
US9422567B2 (en) 2016-08-23
US8709782B2 (en) 2014-04-29
TW200641124A (en) 2006-12-01
US20170002367A1 (en) 2017-01-05
CN103146034B (zh) 2016-01-20
US20110281334A1 (en) 2011-11-17
US8716003B2 (en) 2014-05-06
US10351858B2 (en) 2019-07-16
US20130203153A1 (en) 2013-08-08
US20180127762A1 (en) 2018-05-10
CN103087941A (zh) 2013-05-08
US7888333B2 (en) 2011-02-15
WO2006096269A3 (en) 2008-12-04
CN103087940B (zh) 2015-05-13
TWI467015B (zh) 2015-01-01

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Legal Events

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FG Grant, registration
FD Application declared void or lapsed, e.g., due to non-payment of fee