AR040004A1 - Variantes de pectatoliasa - Google Patents
Variantes de pectatoliasaInfo
- Publication number
- AR040004A1 AR040004A1 ARP030101676A AR040004A1 AR 040004 A1 AR040004 A1 AR 040004A1 AR P030101676 A ARP030101676 A AR P030101676A AR 040004 A1 AR040004 A1 AR 040004A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- pectatoliase
- occupies
- precursor enzyme
- variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere a variantes de pectatoliasa que exhiben alteraciones con respecto a una enzima precursora que manifiesta actividad de pectatoliasa como su mayor actividad enzimática; a un método para producir dichas enzimas; y a métodos para emplear tales enzimas en la industria textil, de detergentes y de procesamiento de fibras celulósicas. En comparación con la enzima precursora, las variantes de pectatoliasa de la presente exhiben estabilidad mejorada en detergentes. Reivindicación 1: una variante de enzima precursora que tiene actividad de pectatoliasa (EC 4.2.2.2) y comprende una alteración en una o más posiciones del grupo que consiste en las posiciones número: 5, 9, 11,26, 28, 30, 31, 37, 40, 45, 46, 47, 48, 49, 50, 51, 52, 54, 61, 64, 68, 69, 70, 71, 74, 75, 76, 79, 86, 87, 91, 99, 105, 106, 107, 111, 115, 116, 118, 122, 123, 134, 136, 139, 140, 141, 146, 148, 156, 158, 170, 182, 185, 186, 189, 193, 194, 196, 199, 201, 202, 204, 213, 215, 218, 224, 228, 229, 234, 235, 237, 251, 256, 257, 258, 272, 277, 286, 295, 298, 301, 302, 303, 305, 307, 308, 314, 316, 323, 324, 326, 331, 333, 334, 335, 336, 337, 338, 339, 340, 341, 349, 356, 357, 363, 366, 378, 381, 384, 386, 387, 389, 390, 391, 393 y 397, siendo la(s) alteración(ciones) independientemente (i) una inserción de un aminoácido que corriente abajo del aminoácido que ocupa la posición; (ii) una supresión del aminoácido que ocupa la posición; o (iii) una sustitución del aminoácido que ocupa la posición con un aminoácido diferente, y en donde cada posición corresponde a una posición de la secuencia de aminoácidos de la pectatoliasa que tiene la secuencia de aminoácidos de SEQ ID NO: 2, y en donde la enzima precursora es la pectatoliasa mostrada en la secuencia SEQ ID NO: 2 o una pectatoliasa que tiene una identidad de por lo menos el 65% respecto de la secuencia de aminoácidos de SEQ ID NO: 2. Reivindicación 12: Un polinucleótido que codifica la variante de cualquiera de las reivindicaciones precedentes. Reivindicación 13: Un vector de expresión que comprende el polinucleótido de la reivindicación 12. Reivindicación 14: Una célula huésped microbiana transformada con el vector de expresión de la reivindicación 13.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200200746 | 2002-05-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR040004A1 true AR040004A1 (es) | 2005-03-09 |
Family
ID=29414636
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030101676 AR040004A1 (es) | 2002-05-14 | 2003-05-14 | Variantes de pectatoliasa |
| ARP130100157 AR089755A2 (es) | 2002-05-14 | 2013-01-18 | Variantes de pectatoliasa |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130100157 AR089755A2 (es) | 2002-05-14 | 2013-01-18 | Variantes de pectatoliasa |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US7601529B2 (es) |
| EP (1) | EP1506292B2 (es) |
| JP (1) | JP4523404B2 (es) |
| CN (1) | CN100482791C (es) |
| AR (2) | AR040004A1 (es) |
| AT (1) | ATE494369T1 (es) |
| AU (1) | AU2003223931A1 (es) |
| BR (1) | BRPI0309937B1 (es) |
| DE (1) | DE60335620D1 (es) |
| ES (1) | ES2359381T5 (es) |
| MX (1) | MXPA04011011A (es) |
| WO (1) | WO2003095638A1 (es) |
Families Citing this family (95)
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| EP2989117B1 (en) | 2013-04-23 | 2019-06-12 | Novozymes A/S | Liquid automatic dish washing detergent compositions with stabilised subtilisin |
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| EP3907271A1 (en) | 2020-05-07 | 2021-11-10 | Novozymes A/S | Cleaning composition, use and method of cleaning |
| PH12023550203A1 (en) | 2020-07-27 | 2024-06-24 | Unilever Ip Holdings B V | Use of an enzyme and surfactant for inhibiting microorganisms |
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| CN115772531B (zh) * | 2022-11-11 | 2024-11-12 | 吉林农业大学 | 一种果胶酸裂解酶基因突变体及其克隆方法和应用 |
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| WO2025132258A1 (en) | 2023-12-20 | 2025-06-26 | Basf Se | Stabilized enzyme composition comprising a protease |
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| US6607902B2 (en) * | 2000-05-04 | 2003-08-19 | Novozymes A/S | Cell-wall degrading enzyme variants |
| MXPA03000435A (es) * | 2000-07-19 | 2004-04-02 | Novozymes As | Variantes de enzimas que degradan la pared celular. |
| US7611882B2 (en) * | 2001-05-14 | 2009-11-03 | Novozymes A/S | Detergent compositions comprising Bacillus subtilis pectate lyases |
| BR0215220A (pt) * | 2001-12-21 | 2007-01-09 | Novozymes Biopolymer As | método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado |
| DK1995319T3 (da) * | 2002-04-10 | 2011-12-05 | Novozymes As | Forbedret bacillusværtscelle |
| ES2359381T5 (es) * | 2002-05-14 | 2014-07-10 | Novozymes A/S | Variantes de pectato liasa |
-
2003
- 2003-05-14 ES ES03720292.6T patent/ES2359381T5/es not_active Expired - Lifetime
- 2003-05-14 AT AT03720292T patent/ATE494369T1/de not_active IP Right Cessation
- 2003-05-14 JP JP2004503631A patent/JP4523404B2/ja not_active Expired - Fee Related
- 2003-05-14 AU AU2003223931A patent/AU2003223931A1/en not_active Abandoned
- 2003-05-14 MX MXPA04011011A patent/MXPA04011011A/es active IP Right Grant
- 2003-05-14 AR ARP030101676 patent/AR040004A1/es active IP Right Grant
- 2003-05-14 BR BRPI0309937A patent/BRPI0309937B1/pt not_active IP Right Cessation
- 2003-05-14 US US10/513,951 patent/US7601529B2/en not_active Expired - Lifetime
- 2003-05-14 EP EP20030720292 patent/EP1506292B2/en not_active Expired - Lifetime
- 2003-05-14 DE DE60335620T patent/DE60335620D1/de not_active Expired - Lifetime
- 2003-05-14 WO PCT/DK2003/000316 patent/WO2003095638A1/en not_active Ceased
- 2003-05-14 CN CNB038138050A patent/CN100482791C/zh not_active Expired - Lifetime
-
2009
- 2009-06-10 US US12/481,922 patent/US8288144B2/en not_active Expired - Lifetime
-
2012
- 2012-09-06 US US13/605,455 patent/US8563290B2/en not_active Expired - Fee Related
-
2013
- 2013-01-18 AR ARP130100157 patent/AR089755A2/es unknown
- 2013-09-13 US US14/026,491 patent/US9005950B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0309937B1 (pt) | 2016-05-31 |
| US8563290B2 (en) | 2013-10-22 |
| US9005950B2 (en) | 2015-04-14 |
| EP1506292B2 (en) | 2014-04-09 |
| WO2003095638A1 (en) | 2003-11-20 |
| BR0309937A (pt) | 2005-02-09 |
| ATE494369T1 (de) | 2011-01-15 |
| AU2003223931A8 (en) | 2003-11-11 |
| AU2003223931A1 (en) | 2003-11-11 |
| EP1506292A1 (en) | 2005-02-16 |
| US20090247448A1 (en) | 2009-10-01 |
| MXPA04011011A (es) | 2005-02-14 |
| US20140005093A1 (en) | 2014-01-02 |
| US20060089283A1 (en) | 2006-04-27 |
| US20130012424A1 (en) | 2013-01-10 |
| EP1506292B1 (en) | 2011-01-05 |
| ES2359381T5 (es) | 2014-07-10 |
| JP2005525118A (ja) | 2005-08-25 |
| US7601529B2 (en) | 2009-10-13 |
| CN1662650A (zh) | 2005-08-31 |
| ES2359381T3 (es) | 2011-05-23 |
| DE60335620D1 (de) | 2011-02-17 |
| US8288144B2 (en) | 2012-10-16 |
| CN100482791C (zh) | 2009-04-29 |
| AR089755A2 (es) | 2014-09-17 |
| JP4523404B2 (ja) | 2010-08-11 |
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