AR040004A1 - PECTATOLIASA VARIANTS - Google Patents
PECTATOLIASA VARIANTSInfo
- Publication number
- AR040004A1 AR040004A1 ARP030101676A AR040004A1 AR 040004 A1 AR040004 A1 AR 040004A1 AR P030101676 A ARP030101676 A AR P030101676A AR 040004 A1 AR040004 A1 AR 040004A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- pectatoliase
- occupies
- precursor enzyme
- variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere a variantes de pectatoliasa que exhiben alteraciones con respecto a una enzima precursora que manifiesta actividad de pectatoliasa como su mayor actividad enzimática; a un método para producir dichas enzimas; y a métodos para emplear tales enzimas en la industria textil, de detergentes y de procesamiento de fibras celulósicas. En comparación con la enzima precursora, las variantes de pectatoliasa de la presente exhiben estabilidad mejorada en detergentes. Reivindicación 1: una variante de enzima precursora que tiene actividad de pectatoliasa (EC 4.2.2.2) y comprende una alteración en una o más posiciones del grupo que consiste en las posiciones número: 5, 9, 11,26, 28, 30, 31, 37, 40, 45, 46, 47, 48, 49, 50, 51, 52, 54, 61, 64, 68, 69, 70, 71, 74, 75, 76, 79, 86, 87, 91, 99, 105, 106, 107, 111, 115, 116, 118, 122, 123, 134, 136, 139, 140, 141, 146, 148, 156, 158, 170, 182, 185, 186, 189, 193, 194, 196, 199, 201, 202, 204, 213, 215, 218, 224, 228, 229, 234, 235, 237, 251, 256, 257, 258, 272, 277, 286, 295, 298, 301, 302, 303, 305, 307, 308, 314, 316, 323, 324, 326, 331, 333, 334, 335, 336, 337, 338, 339, 340, 341, 349, 356, 357, 363, 366, 378, 381, 384, 386, 387, 389, 390, 391, 393 y 397, siendo la(s) alteración(ciones) independientemente (i) una inserción de un aminoácido que corriente abajo del aminoácido que ocupa la posición; (ii) una supresión del aminoácido que ocupa la posición; o (iii) una sustitución del aminoácido que ocupa la posición con un aminoácido diferente, y en donde cada posición corresponde a una posición de la secuencia de aminoácidos de la pectatoliasa que tiene la secuencia de aminoácidos de SEQ ID NO: 2, y en donde la enzima precursora es la pectatoliasa mostrada en la secuencia SEQ ID NO: 2 o una pectatoliasa que tiene una identidad de por lo menos el 65% respecto de la secuencia de aminoácidos de SEQ ID NO: 2. Reivindicación 12: Un polinucleótido que codifica la variante de cualquiera de las reivindicaciones precedentes. Reivindicación 13: Un vector de expresión que comprende el polinucleótido de la reivindicación 12. Reivindicación 14: Una célula huésped microbiana transformada con el vector de expresión de la reivindicación 13.This refers to variants of pectatoliase that exhibit alterations with respect to a precursor enzyme that manifests pectatoliase activity as its greatest enzymatic activity; to a method to produce said enzymes; and to methods for employing such enzymes in the textile, detergent and cellulosic fiber processing industry. In comparison to the precursor enzyme, the pectatoliase variants of the present exhibit improved stability in detergents. Claim 1: a precursor enzyme variant that has pectatoliase activity (EC 4.2.2.2) and comprises an alteration in one or more positions of the group consisting of positions number: 5, 9, 11,26, 28, 30, 31 , 37, 40, 45, 46, 47, 48, 49, 50, 51, 52, 54, 61, 64, 68, 69, 70, 71, 74, 75, 76, 79, 86, 87, 91, 99 , 105, 106, 107, 111, 115, 116, 118, 122, 123, 134, 136, 139, 140, 141, 146, 148, 156, 158, 170, 182, 185, 186, 189, 193, 194 , 196, 199, 201, 202, 204, 213, 215, 218, 224, 228, 229, 234, 235, 237, 251, 256, 257, 258, 272, 277, 286, 295, 298, 301, 302 , 303, 305, 307, 308, 314, 316, 323, 324, 326, 331, 333, 334, 335, 336, 337, 338, 339, 340, 341, 349, 356, 357, 363, 366, 378 , 381, 384, 386, 387, 389, 390, 391, 393 and 397, the alteration (s) being independently (i) an insertion of an amino acid that flows downstream of the amino acid that occupies the position; (ii) a suppression of the amino acid that occupies the position; or (iii) a substitution of the amino acid that occupies the position with a different amino acid, and where each position corresponds to a position of the amino acid sequence of the pectatoliase having the amino acid sequence of SEQ ID NO: 2, and wherein the precursor enzyme is the pectatoliase shown in the sequence SEQ ID NO: 2 or a pectatoliase having an identity of at least 65% with respect to the amino acid sequence of SEQ ID NO: 2. Claim 12: A polynucleotide encoding the variant of any of the preceding claims. Claim 13: An expression vector comprising the polynucleotide of claim 12. Claim 14: A microbial host cell transformed with the expression vector of claim 13.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200200746 | 2002-05-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR040004A1 true AR040004A1 (en) | 2005-03-09 |
Family
ID=29414636
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030101676 AR040004A1 (en) | 2002-05-14 | 2003-05-14 | PECTATOLIASA VARIANTS |
| ARP130100157 AR089755A2 (en) | 2002-05-14 | 2013-01-18 | PECTATOLIASA VARIANTS |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130100157 AR089755A2 (en) | 2002-05-14 | 2013-01-18 | PECTATOLIASA VARIANTS |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US7601529B2 (en) |
| EP (1) | EP1506292B2 (en) |
| JP (1) | JP4523404B2 (en) |
| CN (1) | CN100482791C (en) |
| AR (2) | AR040004A1 (en) |
| AT (1) | ATE494369T1 (en) |
| AU (1) | AU2003223931A1 (en) |
| BR (1) | BRPI0309937B1 (en) |
| DE (1) | DE60335620D1 (en) |
| ES (1) | ES2359381T5 (en) |
| MX (1) | MXPA04011011A (en) |
| WO (1) | WO2003095638A1 (en) |
Families Citing this family (95)
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| CN115772531B (en) * | 2022-11-11 | 2024-11-12 | 吉林农业大学 | A pectate lyase gene mutant and its cloning method and application |
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| WO2025202370A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
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| US6607902B2 (en) * | 2000-05-04 | 2003-08-19 | Novozymes A/S | Cell-wall degrading enzyme variants |
| MXPA03000435A (en) * | 2000-07-19 | 2004-04-02 | Novozymes As | Cell-wall degrading enzyme variants. |
| US7611882B2 (en) * | 2001-05-14 | 2009-11-03 | Novozymes A/S | Detergent compositions comprising Bacillus subtilis pectate lyases |
| BR0215220A (en) * | 2001-12-21 | 2007-01-09 | Novozymes Biopolymer As | method for producing a hyaluronic acid, bacillus host cell, nucleic acid construct, isolated nucleic acid sequence, expression vector, recombinant expression vector, recombinant host cell, method for producing a polypeptide, and isolated polypeptide |
| DK1995319T3 (en) * | 2002-04-10 | 2011-12-05 | Novozymes As | Enhanced bacillus host cell |
| ES2359381T5 (en) * | 2002-05-14 | 2014-07-10 | Novozymes A/S | Variants of pectate lyase |
-
2003
- 2003-05-14 ES ES03720292.6T patent/ES2359381T5/en not_active Expired - Lifetime
- 2003-05-14 AT AT03720292T patent/ATE494369T1/en not_active IP Right Cessation
- 2003-05-14 JP JP2004503631A patent/JP4523404B2/en not_active Expired - Fee Related
- 2003-05-14 AU AU2003223931A patent/AU2003223931A1/en not_active Abandoned
- 2003-05-14 MX MXPA04011011A patent/MXPA04011011A/en active IP Right Grant
- 2003-05-14 AR ARP030101676 patent/AR040004A1/en active IP Right Grant
- 2003-05-14 BR BRPI0309937A patent/BRPI0309937B1/en not_active IP Right Cessation
- 2003-05-14 US US10/513,951 patent/US7601529B2/en not_active Expired - Lifetime
- 2003-05-14 EP EP20030720292 patent/EP1506292B2/en not_active Expired - Lifetime
- 2003-05-14 DE DE60335620T patent/DE60335620D1/en not_active Expired - Lifetime
- 2003-05-14 WO PCT/DK2003/000316 patent/WO2003095638A1/en not_active Ceased
- 2003-05-14 CN CNB038138050A patent/CN100482791C/en not_active Expired - Lifetime
-
2009
- 2009-06-10 US US12/481,922 patent/US8288144B2/en not_active Expired - Lifetime
-
2012
- 2012-09-06 US US13/605,455 patent/US8563290B2/en not_active Expired - Fee Related
-
2013
- 2013-01-18 AR ARP130100157 patent/AR089755A2/en unknown
- 2013-09-13 US US14/026,491 patent/US9005950B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0309937B1 (en) | 2016-05-31 |
| US8563290B2 (en) | 2013-10-22 |
| US9005950B2 (en) | 2015-04-14 |
| EP1506292B2 (en) | 2014-04-09 |
| WO2003095638A1 (en) | 2003-11-20 |
| BR0309937A (en) | 2005-02-09 |
| ATE494369T1 (en) | 2011-01-15 |
| AU2003223931A8 (en) | 2003-11-11 |
| AU2003223931A1 (en) | 2003-11-11 |
| EP1506292A1 (en) | 2005-02-16 |
| US20090247448A1 (en) | 2009-10-01 |
| MXPA04011011A (en) | 2005-02-14 |
| US20140005093A1 (en) | 2014-01-02 |
| US20060089283A1 (en) | 2006-04-27 |
| US20130012424A1 (en) | 2013-01-10 |
| EP1506292B1 (en) | 2011-01-05 |
| ES2359381T5 (en) | 2014-07-10 |
| JP2005525118A (en) | 2005-08-25 |
| US7601529B2 (en) | 2009-10-13 |
| CN1662650A (en) | 2005-08-31 |
| ES2359381T3 (en) | 2011-05-23 |
| DE60335620D1 (en) | 2011-02-17 |
| US8288144B2 (en) | 2012-10-16 |
| CN100482791C (en) | 2009-04-29 |
| AR089755A2 (en) | 2014-09-17 |
| JP4523404B2 (en) | 2010-08-11 |
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