AR010993A1 - VECTOR RECOMBINANTE; CELULA HUÉSPED RECOMBINANTE, METODO PARA USAR Y METODO PARA DETECTAR UN SEGMENTO DE ACIDOS NUCLEICOS QUE CODIFICA UNA PROTEINA CRISTALINA; CONJUNTO DE ELEMENTOS, ANTICUERPO PURIFICADO; CELULA RECOMBINANTE BACILLUS THURINGIENSIS, COMPOSICIoN INSECTICIDA Y METODO PARA PREPARAR UNA - Google Patents
VECTOR RECOMBINANTE; CELULA HUÉSPED RECOMBINANTE, METODO PARA USAR Y METODO PARA DETECTAR UN SEGMENTO DE ACIDOS NUCLEICOS QUE CODIFICA UNA PROTEINA CRISTALINA; CONJUNTO DE ELEMENTOS, ANTICUERPO PURIFICADO; CELULA RECOMBINANTE BACILLUS THURINGIENSIS, COMPOSICIoN INSECTICIDA Y METODO PARA PREPARAR UNAInfo
- Publication number
- AR010993A1 AR010993A1 ARP970104383A ARP970104383A AR010993A1 AR 010993 A1 AR010993 A1 AR 010993A1 AR P970104383 A ARP970104383 A AR P970104383A AR P970104383 A ARP970104383 A AR P970104383A AR 010993 A1 AR010993 A1 AR 010993A1
- Authority
- AR
- Argentina
- Prior art keywords
- recombinant
- cell
- nucleic acid
- larvae
- cryet34
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 241000193388 Bacillus thuringiensis Species 0.000 title abstract 3
- 229940097012 bacillus thuringiensis Drugs 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 239000002917 insecticide Substances 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 101710151559 Crystal protein Proteins 0.000 abstract 4
- 241000254101 Popillia japonica Species 0.000 abstract 3
- 241000254113 Tribolium castaneum Species 0.000 abstract 3
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 abstract 1
- 241000254173 Coleoptera Species 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 abstract 1
- 230000000749 insecticidal effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 108700012359 toxins Proteins 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
- G01N2333/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se revelan cepas de Bacillus Thuringiensis que comprenden nuevas proteínas cristalinas que tienen actividad insecticida contra insectos coleopteros entrelos que se incluyen las larvas del escarabajo colorado de la harina (Tribolium castaneum) ylas larvas del escarabajo japonés (Popillia japonica). También sedescriben genes de nuevas toxinas cristalinas de B. thuringiensis, que se denominan cryET33 y cryET34, que codifican las proteínas cristalinas toxicaspara los coleopteros, laproteín a cristalina CryET33 (29-kDa), y el gen cryET34 codifica la proteína cristalina de 14-kDa CryET34. Las proteínas cristalinasCryET33 y CryET34 son toxicas para las larvas del escarabajo colorado de la harina y las larvas del escarabajojaponés. Ta mbién se revelan métodos defabricar y usar células transgénicas que comprenden las nuevas secuencias de ácidos nucleicos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/718,905 US6063756A (en) | 1996-09-24 | 1996-09-24 | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR010993A1 true AR010993A1 (es) | 2000-08-02 |
Family
ID=24888036
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970104383A AR010993A1 (es) | 1996-09-24 | 1997-09-24 | VECTOR RECOMBINANTE; CELULA HUÉSPED RECOMBINANTE, METODO PARA USAR Y METODO PARA DETECTAR UN SEGMENTO DE ACIDOS NUCLEICOS QUE CODIFICA UNA PROTEINA CRISTALINA; CONJUNTO DE ELEMENTOS, ANTICUERPO PURIFICADO; CELULA RECOMBINANTE BACILLUS THURINGIENSIS, COMPOSICIoN INSECTICIDA Y METODO PARA PREPARAR UNA |
| ARP060102842A AR054156A2 (es) | 1996-09-24 | 2006-06-30 | Celula vegetal recombinante transformada para expresar un polipeptido insecticida, metodo para preparar dicha celula , anticuerpo purificado metodo de deteccion de un polipeptido y conjunto de elementos (kit) de inmunodeteccion |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP060102842A AR054156A2 (es) | 1996-09-24 | 2006-06-30 | Celula vegetal recombinante transformada para expresar un polipeptido insecticida, metodo para preparar dicha celula , anticuerpo purificado metodo de deteccion de un polipeptido y conjunto de elementos (kit) de inmunodeteccion |
Country Status (19)
| Country | Link |
|---|---|
| US (8) | US6063756A (es) |
| EP (1) | EP1015592B1 (es) |
| JP (1) | JP2001523944A (es) |
| KR (1) | KR20000048593A (es) |
| CN (1) | CN100473725C (es) |
| AR (2) | AR010993A1 (es) |
| AT (1) | ATE324447T1 (es) |
| AU (1) | AU741704B2 (es) |
| BR (1) | BR9713219B1 (es) |
| CA (1) | CA2267665C (es) |
| CO (1) | CO4650229A1 (es) |
| DE (1) | DE69735776T2 (es) |
| IL (1) | IL129160A0 (es) |
| MX (1) | MXPA99002819A (es) |
| PL (1) | PL189474B1 (es) |
| TR (1) | TR199900650T2 (es) |
| UA (1) | UA75317C2 (es) |
| UY (1) | UY24725A1 (es) |
| WO (1) | WO1998013498A1 (es) |
Families Citing this family (153)
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|---|---|---|---|---|
| US6063756A (en) * | 1996-09-24 | 2000-05-16 | Monsanto Company | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
| WO2001011076A1 (en) * | 1999-08-04 | 2001-02-15 | Tosk, Inc. | In vivo high throughput toxicology screening method |
| US6365129B1 (en) | 1999-08-04 | 2002-04-02 | Tosk, Inc. | Invivo high throughput toxicology screening method |
| AU2001290919A1 (en) | 2000-09-12 | 2002-03-26 | Monsanto Technology Llc | Insect inhibitory bacillus thuringiensis proteins, fusions, and methods of use therefor |
| US9816104B2 (en) * | 2000-10-06 | 2017-11-14 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge as a seed blend |
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| US20050033137A1 (en) * | 2002-10-25 | 2005-02-10 | The Regents Of The University Of Michigan | Ablation catheters and methods for their use |
| EP1613730A4 (en) | 2003-03-28 | 2007-12-05 | Monsanto Technology Llc | NEW PLANT PROMOTORS FOR USE IN THE EARLY SEED DEVELOPMENT PHASE |
| US8039229B2 (en) * | 2003-07-25 | 2011-10-18 | University Of Georgia Research Foundation, Inc. | Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods |
| PL1658364T3 (pl) | 2003-08-25 | 2017-10-31 | Monsanto Technology Llc | Elementy regulatorowe tubuliny do stosowania w roślinach |
| CA2547933C (en) | 2003-12-16 | 2014-08-12 | Monsanto Technology Llc | Secreted insecticidal protein and gene compositions from bacillus thuringiensis and uses therefor |
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| AU2006217847B2 (en) | 2005-02-26 | 2011-04-07 | Basf Plant Science Gmbh | Expression cassettes for seed-preferential expression in plants |
| CN101198701B (zh) | 2005-04-19 | 2013-04-24 | 巴斯福植物科学有限公司 | 单子叶植物中的淀粉胚乳特异性和/或萌芽胚特异性表达 |
| BRPI0614070A2 (pt) | 2005-05-10 | 2018-07-31 | Basf Plant Science Gmbh | cassetes de expressão, sequência de nucleotídeos isolada, seqüência sintética reguladora de transcrição, métodos para proporcionar uma seqüência sintética de nucleotídeos reguladora de transcrição, e um cassete de expressão, vetor, organismo não-humano ou célula hospedeira transgênico(a), e, célula de planta ou planta transgênica |
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| EP2455391A3 (en) | 2006-06-15 | 2012-08-22 | Athenix Corporation | A family of pesticidal proteins and methods for their use |
| CN101631868B (zh) | 2007-02-16 | 2016-02-10 | 巴斯福植物科学有限公司 | 用于在单子叶植物中调节胚特异性表达的核酸序列 |
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