AR006016A1 - Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodo - Google Patents
Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodoInfo
- Publication number
- AR006016A1 AR006016A1 ARP970100781A ARP970100781A AR006016A1 AR 006016 A1 AR006016 A1 AR 006016A1 AR P970100781 A ARP970100781 A AR P970100781A AR P970100781 A ARP970100781 A AR P970100781A AR 006016 A1 AR006016 A1 AR 006016A1
- Authority
- AR
- Argentina
- Prior art keywords
- pylori
- complex
- antibody
- kit
- antigen
- Prior art date
Links
- 230000002550 fecal effect Effects 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 3
- 239000000427 antigen Substances 0.000 abstract 4
- 102000036639 antigens Human genes 0.000 abstract 4
- 108091007433 antigens Proteins 0.000 abstract 4
- 239000003085 diluting agent Substances 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56922—Campylobacter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se trata de un proceso para determinar H.piloris muestras fecales, que comprende: (a) dispersar la muestra fecal en un diluyente; (b) poner encontacto la muestra fecal en el diluyente, con un primer anticuerpo policlonal para el antígeno deH.p ilori, para formar un complejo del anticuerpo y delantígeno; (c) separar dicha muestra y dicho complejo; (d) exponer el complejo a un segundo anticuerpo policlonal para dicho antígeno y una porción delanticuerpo que reacciona con dicho complejo,esta ndo uno de dichos anticuerpos primero y segundo, vinculado a un vehículo sólido, y estando el otro, marcadacon un agente de detección; y (e)determinar la cantidad del anticuerpo etiquetado y a su vez determinar la presencia delantígeno del H. pilori endicha muestra fecal. También se propone un kit para detectar H. pilori mediante inmunoensayos aplicado sobre muestras fecales.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/647,115 US5716791A (en) | 1996-05-09 | 1996-05-09 | Immunoassay for H. pylori in fecal specimens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR006016A1 true AR006016A1 (es) | 1999-07-21 |
Family
ID=24595765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970100781A AR006016A1 (es) | 1996-05-09 | 1997-02-27 | Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodo |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US5716791A (es) |
| EP (1) | EP0806667B2 (es) |
| JP (1) | JP3043999B2 (es) |
| CN (1) | CN1271408C (es) |
| AR (1) | AR006016A1 (es) |
| AT (1) | ATE211552T1 (es) |
| AU (1) | AU698513B2 (es) |
| CA (1) | CA2198336C (es) |
| DE (2) | DE69709434T3 (es) |
| ES (1) | ES2132044T5 (es) |
| GR (1) | GR990300022T1 (es) |
| MX (1) | MX9701877A (es) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
| CA2224551A1 (en) * | 1998-02-25 | 1999-08-25 | Alex Hongsheng Chang | A quick in situ diagnostic method for detecting alimentary diseases |
| DK1974747T3 (da) | 1998-08-11 | 2012-09-17 | Biogen Idec Inc | Kombinationsterapier for B-celle-lymfomer omfattende indgivelse af anti-CD20-antistof |
| DK1125130T3 (da) * | 1998-10-29 | 2006-11-27 | Dakocytomation Denmark As | Påvisning af syreresistente mikroorganismer i afföringen |
| US8557244B1 (en) | 1999-08-11 | 2013-10-15 | Biogen Idec Inc. | Treatment of aggressive non-Hodgkins lymphoma with anti-CD20 antibody |
| CA2387473C (en) | 1999-10-12 | 2010-02-02 | Connex Gesellschaft Zur Optimierung Von Forschung Und Entwicklung Mbh | Improved method for the detection of acid resistant microorganisms in a stool |
| DE60045870D1 (de) | 1999-10-29 | 2011-06-01 | Wakamoto Pharma Co Ltd | Monoklonaler antikörper, hybridoma, immuntestverfahren und diagnosekit |
| JP3504633B2 (ja) * | 1999-10-29 | 2004-03-08 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法及び検査試薬 |
| WO2001040801A2 (en) * | 1999-12-03 | 2001-06-07 | Meridian Bioscience, Inc. | Diagnosis and treatment for helicobacter pylori induced colic |
| TWI237695B (en) * | 1999-12-14 | 2005-08-11 | Joy Biomedical Corp | Helicobacter pylori antigens in blood |
| DE10006432A1 (de) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Verfahren zum Nachweis von Helicobacter pylori in Stuhl- und Speichelproben |
| DE60138981D1 (de) * | 2000-05-18 | 2009-07-30 | Meridian Bioscience Inc | Immunoassay für H. pylori in Fäkalienproben mit Hilfe von gattungsspezifischen Antikörpern |
| WO2002014541A1 (fr) * | 2000-08-11 | 2002-02-21 | Wakamoto Pharmaceutical Co., Ltd. | Methode d'examen d'une infection a $m(f)i$m(g)helicobacter pylori$m(f)/i$m(g) et kit de diagnostic |
| JP4443117B2 (ja) * | 2001-04-23 | 2010-03-31 | わかもと製薬株式会社 | イムノクロマトグラフィー試験片及び診断キット |
| JP4763149B2 (ja) * | 2001-05-10 | 2011-08-31 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法 |
| FI118061B (fi) | 2001-09-24 | 2007-06-15 | Beanor Oy | Menetelmä ja bioanturi analyysiä varten |
| FI115166B (fi) | 2001-12-31 | 2005-03-15 | Biofons Oy | Diagnostisia menetelmiä |
| DE10219741A1 (de) * | 2002-05-02 | 2003-11-13 | Georg S Wengler | Verfahren zur Vorbehandlung von Stuhlproben |
| KR20040046427A (ko) * | 2002-11-27 | 2004-06-05 | 주식회사 비엘에스 | 분변 시료 중의 헬리코박터 피로리의 검출방법 |
| JP2010510526A (ja) * | 2006-11-22 | 2010-04-02 | スリーエム イノベイティブ プロパティズ カンパニー | 細菌全細胞を捕捉する方法及び細菌について試料を分析する方法 |
| US8025880B2 (en) * | 2007-03-01 | 2011-09-27 | Helicure Ab | Immunoglobulin against Helicobacter pylori |
| US20090263905A1 (en) * | 2008-04-18 | 2009-10-22 | Kim Scheuringer | Detection test assembly for detecting the presence of a substance in a sample |
| US9110053B2 (en) * | 2010-08-20 | 2015-08-18 | Agilent Technologies, Inc. | Dried blood spotting paper device and method |
| US8541180B1 (en) | 2010-10-11 | 2013-09-24 | Genova Diagnostics, Inc. | Compositions and methods for assessing gastrointestinal health |
| CN102565391A (zh) * | 2011-12-23 | 2012-07-11 | 王滔 | 胃黏膜样品中幽门螺杆菌的免疫测定法 |
| CN103293305A (zh) * | 2012-03-05 | 2013-09-11 | 美利泰格诊断试剂(嘉兴)有限公司 | 口腔螺旋杆菌感染检测方法及用于检测的唾液试板 |
| CN102980884A (zh) * | 2012-11-29 | 2013-03-20 | 江苏创生生物技术有限公司 | 胃黏膜样品中幽门螺旋杆菌的化学发光免疫测定法 |
| CN103149067A (zh) * | 2013-02-28 | 2013-06-12 | 苏州和锐医药科技有限公司 | 一种粪便蛋白萃取方法及试剂 |
| KR101619862B1 (ko) | 2014-09-30 | 2016-05-12 | 주식회사 녹십자 | 간접효소면역측정법을 이용하는 인간 Fc 포함 단백질의 역가 측정키트 및 이를 이용한 Fc 포함 단백질의 역가 측정방법 |
| DE102015000626A1 (de) * | 2015-01-22 | 2016-07-28 | Kibion Gmbh | Verfahren zum Nachweis von Helicobacter Pylori |
| CN109342722A (zh) * | 2018-09-30 | 2019-02-15 | 深圳市鸿美诊断技术有限公司 | 一种用于定量测定粪便中幽门螺杆菌抗原试剂的制备方法 |
| CN117347629A (zh) * | 2023-09-22 | 2024-01-05 | 重庆新赛亚生物科技有限公司 | 一种微球标记幽门螺杆菌抗体的方法、试剂条及试剂盒 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0063810B1 (en) * | 1981-04-29 | 1986-03-05 | Ciba-Geigy Ag | New devices and kits for immunological analysis |
| EP0232085B1 (en) * | 1986-01-22 | 1993-04-28 | Amoco Corporation | Campylobacter probe |
| US4942126A (en) * | 1986-08-13 | 1990-07-17 | Allegheny-Singer Research Institute | Method of extracting antigens of bacteria |
| EP0281251A3 (en) * | 1987-02-04 | 1988-09-28 | International Immunoassay Laboratories, Inc. | A method for preparing a fecal sample composition for immunoassay testing |
| US4923801A (en) * | 1987-04-13 | 1990-05-08 | The University Of Virginia Alumni Patents Foundation | Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment |
| US5459041A (en) * | 1988-02-18 | 1995-10-17 | Enteric Research Laboratories, Inc. | Campylobacter pylori antigens and uses thereof for detection of Campylobacter pylori infection |
| US4882271A (en) * | 1988-03-10 | 1989-11-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
| US5017342A (en) * | 1988-06-01 | 1991-05-21 | Ortho Diagnostic Systems Inc. | Device for immunoassay determinations |
| US5571674A (en) * | 1989-09-18 | 1996-11-05 | The Trustees Of Columbia University In The City Of New York | DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers |
| AU644121B2 (en) * | 1989-12-04 | 1993-12-02 | Auspharm International Limited | Rapid in vitro test for helicobacter pylori using saliva |
| JP2988635B2 (ja) * | 1990-09-18 | 1999-12-13 | 塩野義製薬株式会社 | ヒトIgEに対するモノクローナル抗体 |
| US5262156A (en) * | 1991-08-12 | 1993-11-16 | Hycor Biomedical, Inc. | Antigenic compositions and their use for the detection of Helicobacter pylori |
| US5420014A (en) * | 1992-04-30 | 1995-05-30 | Auspharm International Ltd. | Rapid in vitro test for helicobacter pylori using saliva |
| JPH07509308A (ja) * | 1992-04-29 | 1995-10-12 | オースファーム インターナショナル リミテッド | ヘリコバクターピロリのinvitro試験 |
| US5403924A (en) * | 1992-10-13 | 1995-04-04 | Vanderbilt University | Taga gene and methods for detecting predisposition to peptic ulceration |
| EP0666987A4 (en) * | 1992-10-30 | 1997-08-27 | T Cell Diagnostics Inc | EVALUATION OF THE TOTAL QUANTITY OF A MOLECULE IN A SAMPLE AND METHODS BASED ON SAME. |
| GB9313437D0 (en) † | 1993-06-30 | 1993-08-11 | Smith Andrew W | Pathogenicity sequences in helicobacter pylori |
| US5486361A (en) * | 1993-10-25 | 1996-01-23 | The United States Of America As Represented By The Department Of Health And Human Services | Hybridomas and monoclonal antibodies that speifically bind to GPIB on platelets and inhibit the binding of thrombin to platelets |
| US5498528A (en) * | 1994-06-10 | 1996-03-12 | King; Wing | Detection of helicobacter pylori |
| US5610060A (en) * | 1994-06-24 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | Isolated Helicobacter hepaticus |
| US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
-
1996
- 1996-05-09 US US08/647,115 patent/US5716791A/en not_active Ceased
-
1997
- 1997-02-24 CA CA002198336A patent/CA2198336C/en not_active Expired - Lifetime
- 1997-02-27 AR ARP970100781A patent/AR006016A1/es active IP Right Grant
- 1997-02-28 AU AU15029/97A patent/AU698513B2/en not_active Expired
- 1997-03-12 CN CN97103112.6A patent/CN1271408C/zh not_active Expired - Lifetime
- 1997-03-12 MX MX9701877A patent/MX9701877A/es unknown
- 1997-03-17 JP JP9062637A patent/JP3043999B2/ja not_active Expired - Fee Related
- 1997-05-08 DE DE69709434T patent/DE69709434T3/de not_active Expired - Lifetime
- 1997-05-08 ES ES97303147T patent/ES2132044T5/es not_active Expired - Lifetime
- 1997-05-08 DE DE0806667T patent/DE806667T1/de active Pending
- 1997-05-08 AT AT97303147T patent/ATE211552T1/de not_active IP Right Cessation
- 1997-05-08 EP EP97303147A patent/EP0806667B2/en not_active Expired - Lifetime
- 1997-07-21 US US08/897,732 patent/US5871942A/en not_active Expired - Lifetime
-
1999
- 1999-01-01 GR GR990300022T patent/GR990300022T1/el unknown
-
2000
- 2000-02-08 US US09/500,365 patent/USRE38088E1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP3043999B2 (ja) | 2000-05-22 |
| ATE211552T1 (de) | 2002-01-15 |
| US5716791A (en) | 1998-02-10 |
| US5871942A (en) | 1999-02-16 |
| EP0806667B1 (en) | 2002-01-02 |
| JPH1010128A (ja) | 1998-01-16 |
| EP0806667B2 (en) | 2008-07-09 |
| DE806667T1 (de) | 1999-10-07 |
| CA2198336A1 (en) | 1997-11-10 |
| DE69709434T3 (de) | 2009-01-02 |
| ES2132044T5 (es) | 2008-12-01 |
| AU1502997A (en) | 1997-11-13 |
| AU698513B2 (en) | 1998-10-29 |
| EP0806667A1 (en) | 1997-11-12 |
| CN1271408C (zh) | 2006-08-23 |
| GR990300022T1 (en) | 1999-07-30 |
| ES2132044T1 (es) | 1999-08-16 |
| USRE38088E1 (en) | 2003-04-22 |
| DE69709434T2 (de) | 2002-09-19 |
| DE69709434D1 (de) | 2002-02-07 |
| CA2198336C (en) | 2002-06-18 |
| MX9701877A (es) | 1998-04-30 |
| CN1165299A (zh) | 1997-11-19 |
| ES2132044T3 (es) | 2002-03-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |