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MX2022005594A - Lavados de salinidad elevada durante cromatografia de intercambio cationico para eliminar impurezas relacionadas con el producto. - Google Patents

Lavados de salinidad elevada durante cromatografia de intercambio cationico para eliminar impurezas relacionadas con el producto.

Info

Publication number
MX2022005594A
MX2022005594A MX2022005594A MX2022005594A MX2022005594A MX 2022005594 A MX2022005594 A MX 2022005594A MX 2022005594 A MX2022005594 A MX 2022005594A MX 2022005594 A MX2022005594 A MX 2022005594A MX 2022005594 A MX2022005594 A MX 2022005594A
Authority
MX
Mexico
Prior art keywords
cation exchange
product
exchange chromatography
high salinity
eliminate impurities
Prior art date
Application number
MX2022005594A
Other languages
English (en)
Inventor
Natalia Gomez
Malhar R Ambhaikar
Benjamin J Tillotson
Luis Diaz
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MX2022005594A publication Critical patent/MX2022005594A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La invención se refiere a métodos para eliminar impurezas relacionadas con el producto de punto isoeléctrico bajo durante operaciones de purificación de intercambio catiónico.
MX2022005594A 2019-11-07 2020-11-04 Lavados de salinidad elevada durante cromatografia de intercambio cationico para eliminar impurezas relacionadas con el producto. MX2022005594A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962931874P 2019-11-07 2019-11-07
PCT/US2020/058774 WO2021091933A1 (en) 2019-11-07 2020-11-04 High salt washes during cation exchange chromatography to remove product-realated impurities

Publications (1)

Publication Number Publication Date
MX2022005594A true MX2022005594A (es) 2022-06-09

Family

ID=73598212

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005594A MX2022005594A (es) 2019-11-07 2020-11-04 Lavados de salinidad elevada durante cromatografia de intercambio cationico para eliminar impurezas relacionadas con el producto.

Country Status (14)

Country Link
US (1) US20220372071A1 (es)
EP (1) EP4055042A1 (es)
JP (1) JP2022554283A (es)
KR (1) KR20220092958A (es)
CN (1) CN114651007A (es)
AR (1) AR120398A1 (es)
AU (1) AU2020377919A1 (es)
BR (1) BR112022008782A2 (es)
CA (1) CA3160208A1 (es)
CL (2) CL2022001169A1 (es)
IL (1) IL292602A (es)
MX (1) MX2022005594A (es)
TW (2) TW202523676A (es)
WO (1) WO2021091933A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021401316A1 (en) * 2020-12-18 2023-07-06 Kiniksa Pharmaceuticals, Gmbh Protein compositions and methods for producing and using the same

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9034298A (en) * 1997-08-25 1999-03-16 Walter H. Runkis A simple, environmentally benign, method for purifying protein
HRP20150282T4 (hr) * 2007-10-30 2018-08-10 Genentech, Inc. Purifikacija protutijela pomoću kromatografije kationskom izmjenom
WO2010127069A1 (en) * 2009-04-29 2010-11-04 Schering Corporation Antibody purification
US20120238730A1 (en) * 2011-03-15 2012-09-20 Abbott Laboratories Integrated approach to the isolation and purification of antibodies
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
EP3145951A1 (en) * 2014-06-24 2017-03-29 InSight Biopharmaceuticals Ltd. Methods of purifying antibodies
PE20170263A1 (es) * 2014-08-04 2017-03-30 Hoffmann La Roche Moleculas biespecificas de union a antigeno activadoras de celulas t
CA2963692A1 (en) * 2014-10-09 2016-04-14 Engmab Ag Bispecific antibodies against cd3epsilon and ror1
JP6699085B2 (ja) * 2015-03-25 2020-05-27 東ソー株式会社 Ｆｃ結合性タンパク質の精製方法
HRP20241323T1 (hr) * 2016-06-17 2024-12-20 F. Hoffmann - La Roche Ag Pročišćavanje multispecifičnih protutijela
US11426706B2 (en) * 2017-06-21 2022-08-30 Cephalon, Inc. Cation exchange chromatography wash buffer
CN110305217B (zh) * 2018-03-27 2022-03-29 广州爱思迈生物医药科技有限公司 双特异性抗体及其应用

Also Published As

Publication number Publication date
TWI878377B (zh) 2025-04-01
TW202523676A (zh) 2025-06-16
WO2021091933A1 (en) 2021-05-14
KR20220092958A (ko) 2022-07-04
BR112022008782A2 (pt) 2022-07-26
CL2024001792A1 (es) 2024-11-08
CL2022001169A1 (es) 2023-01-27
CN114651007A (zh) 2022-06-21
EP4055042A1 (en) 2022-09-14
AR120398A1 (es) 2022-02-09
AU2020377919A1 (en) 2022-06-16
TW202132323A (zh) 2021-09-01
IL292602A (en) 2022-07-01
JP2022554283A (ja) 2022-12-28
US20220372071A1 (en) 2022-11-24
CA3160208A1 (en) 2021-05-14

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