MX2020005724A - Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. - Google Patents
Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.Info
- Publication number
- MX2020005724A MX2020005724A MX2020005724A MX2020005724A MX2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- alpha
- polynucleotides encoding
- variants
- amylase variants
- Prior art date
Links
- 102000004139 alpha-Amylases Human genes 0.000 title abstract 3
- 108090000637 alpha-Amylases Proteins 0.000 title abstract 3
- 229940024171 alpha-amylase Drugs 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title abstract 3
- 108091033319 polynucleotide Proteins 0.000 title abstract 3
- 239000002157 polynucleotide Substances 0.000 title abstract 3
- 238000006467 substitution reaction Methods 0.000 abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a variantes de alfa-amilasa que comprenden sustituciones en las posiciones correspondientes a las posiciones 268 y 293 de SEC. ID NO.: 1, en particular, sustituciones seleccionadas del grupo que consiste en: 268G+293Y; 268G+293F; 268G+293W; 268G+293H; 268G+293A; 268G+293Q; 268A+293Y; 268A+293F; 268A+293W; 268A+293H; 268A+293A; 268A+293Q; 268P+293Y; 268P+293F; 268P+293W; 268P+293H; 268P+293A; 268P+293Q; 268S+293Y; 268S+293F; 268S+293W; 268S+293H; 268S+293A; 268S+293Q; 268T+293Y; 268T+293F; 268T+293W; 268T+293H; 268T+293A; 268T+293Q; 268V+293Y; 268V+293F; 268V+293W; 268V+293H; 268V+293A; 268V+293Q; 268I+293Y; 268I+293F; 268I+293W; 268I+293H; 268I+293A; 268I+293Q; 268L+293Y; 268L+293F; 268L+293W; 268L+293H; 268L+293A; 268L+293Q; 268M+293Y; 268M+293F; 268M+293W; 268M+293H; 268M+293A; 268M+293Q;y donde la variante tiene al menos el 70 %, al menos el 75 %, al menos el 80 %, al menos el 85 %, al menos el 90 %, al menos el 95 %, al menos el 96 %, al menos el 97 %, al menos el 98 % o al menos el 99 %, pero menos del 100 % de identidad de secuencia con una alfa-amilasa precursora seleccionada del grupo que consiste en SEC. ID NO.: 1, SEC. ID NO.: 2, SEC. ID NO.: 3, SEC. ID NO.: 4 y SEC. ID NO.: 18. La presente invención también se refiere apolinucleótidos que codifican las variantes; a construcciones de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762596231P | 2017-12-08 | 2017-12-08 | |
| US201862765268P | 2018-08-20 | 2018-08-20 | |
| PCT/US2018/064415 WO2019113415A1 (en) | 2017-12-08 | 2018-12-07 | Alpha-amylase variants and polynucleotides encoding same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020005724A true MX2020005724A (es) | 2020-08-13 |
Family
ID=64746663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020005724A MX2020005724A (es) | 2017-12-08 | 2018-12-07 | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11384347B2 (es) |
| EP (1) | EP3720956B1 (es) |
| CN (1) | CN111492054A (es) |
| BR (1) | BR112020011173A2 (es) |
| CA (1) | CA3081096A1 (es) |
| DK (1) | DK3720956T5 (es) |
| ES (1) | ES2954431T3 (es) |
| FI (1) | FI3720956T3 (es) |
| MX (1) | MX2020005724A (es) |
| WO (1) | WO2019113415A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230167386A1 (en) * | 2020-05-21 | 2023-06-01 | Dupont Nutrition Biosciences Aps | Uninhibited amylases for brewing with high tannin materials |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| EP4638725A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
| US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
| US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
| JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
| US4560651A (en) | 1981-04-20 | 1985-12-24 | Novo Industri A/S | Debranching enzyme product, preparation and use thereof |
| KR960006119B1 (ko) | 1986-07-09 | 1996-05-09 | 노보 노르디스크 아크티에 셀스카브 | 전분 액화용 알파-아밀라아제 혼합물 |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| DK0477280T3 (es) | 1989-06-13 | 1997-02-10 | Genencor Int | |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| DE69833652T2 (de) | 1997-06-10 | 2006-09-21 | Takara Bio Inc., Otsu | System zum exprimieren einer hyperthermostabilen protease |
| EP2206768B1 (en) | 1997-10-13 | 2015-04-01 | Novozymes A/S | Alpha-amylase mutants |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| DK1165762T3 (da) | 1999-03-30 | 2007-09-03 | Novozymes As | Alpha-amylase varianter |
| EP1250423B1 (en) | 2000-01-12 | 2008-09-03 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| EP1421187B1 (en) | 2001-07-27 | 2007-10-10 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| DK1456369T3 (da) | 2001-12-07 | 2010-03-22 | Novozymes As | Polypeptider med proteaseaktivitet og nukleinsyrer kodende derfor |
| MX2010004674A (es) | 2007-11-05 | 2010-05-20 | Danisco Us Inc | Variantes de alfa-amilasa con propiedades alteradas. |
| BRPI0913402B1 (pt) * | 2008-06-06 | 2019-07-02 | Danisco Us Inc. | Alfa amilases (amys) variantes de geobacillus stearothermophilus com propriedades melhoradas |
| CN102083991A (zh) | 2008-06-23 | 2011-06-01 | 诺维信公司 | 生产发酵产物的方法 |
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| WO2011066576A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| WO2011068803A1 (en) | 2009-12-01 | 2011-06-09 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| EP3101127B1 (en) | 2010-01-04 | 2019-03-20 | Novozymes A/S | Alpha-amylase variants with improved stability |
| WO2011127802A1 (en) | 2010-04-14 | 2011-10-20 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| US9732332B2 (en) | 2010-11-08 | 2017-08-15 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| DK2654567T3 (en) | 2010-12-22 | 2018-06-25 | Novozymes North America Inc | Process for making fermentation products from starch-containing materials |
| EA201490216A1 (ru) | 2011-07-06 | 2014-07-30 | Новозимс А/С | Варианты альфа-амилазы и кодирующие их полинуклеотиды |
| CN103946378B (zh) | 2011-10-11 | 2018-04-24 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| CN103857794B (zh) | 2011-10-17 | 2018-03-20 | 诺维信公司 | α‑淀粉酶变体以及编码它们的多核苷酸 |
| EP4253534A3 (en) | 2011-10-17 | 2024-02-07 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2013082486A1 (en) | 2011-12-02 | 2013-06-06 | Novozymes A/S | Processes for producing fermentation products |
| EP3060659B1 (en) | 2013-10-03 | 2019-05-29 | Danisco US Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
| WO2017015329A1 (en) | 2015-07-23 | 2017-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
-
2018
- 2018-12-07 MX MX2020005724A patent/MX2020005724A/es unknown
- 2018-12-07 FI FIEP18822573.4T patent/FI3720956T3/fi active
- 2018-12-07 CN CN201880078038.7A patent/CN111492054A/zh active Pending
- 2018-12-07 BR BR112020011173-2A patent/BR112020011173A2/pt unknown
- 2018-12-07 ES ES18822573T patent/ES2954431T3/es active Active
- 2018-12-07 DK DK18822573.4T patent/DK3720956T5/da active
- 2018-12-07 EP EP18822573.4A patent/EP3720956B1/en active Active
- 2018-12-07 WO PCT/US2018/064415 patent/WO2019113415A1/en not_active Ceased
- 2018-12-07 CA CA3081096A patent/CA3081096A1/en active Pending
- 2018-12-07 US US16/770,853 patent/US11384347B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2954431T3 (es) | 2023-11-22 |
| CA3081096A1 (en) | 2019-06-13 |
| EP3720956B1 (en) | 2023-06-07 |
| CN111492054A (zh) | 2020-08-04 |
| DK3720956T5 (da) | 2024-08-26 |
| EP3720956A1 (en) | 2020-10-14 |
| BR112020011173A2 (pt) | 2020-11-17 |
| FI3720956T3 (fi) | 2023-08-11 |
| DK3720956T3 (da) | 2023-09-04 |
| US20200392474A1 (en) | 2020-12-17 |
| US11384347B2 (en) | 2022-07-12 |
| WO2019113415A1 (en) | 2019-06-13 |
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