MX2020005458A - Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. - Google Patents
Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.Info
- Publication number
- MX2020005458A MX2020005458A MX2020005458A MX2020005458A MX2020005458A MX 2020005458 A MX2020005458 A MX 2020005458A MX 2020005458 A MX2020005458 A MX 2020005458A MX 2020005458 A MX2020005458 A MX 2020005458A MX 2020005458 A MX2020005458 A MX 2020005458A
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- alpha
- polynucleotides encoding
- variants
- amylase variants
- Prior art date
Links
- 102000004139 alpha-Amylases Human genes 0.000 title abstract 3
- 108090000637 alpha-Amylases Proteins 0.000 title abstract 3
- 229940024171 alpha-amylase Drugs 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title abstract 3
- 108091033319 polynucleotide Proteins 0.000 title abstract 3
- 239000002157 polynucleotide Substances 0.000 title abstract 3
- 238000006467 substitution reaction Methods 0.000 abstract 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Emergency Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a variantes de alfaamilasa que comprenden una sustitución en una posición correspondiente a la posición 188 y al menos una sustitución adicional en una posición correspondiente a la posición 242 o 279 o 275 de SEC. ID NO.: 1, en particular, una o más combinaciones de sustituciones seleccionadas del grupo que consiste en E188P+S242Y, E188P+S242F, E188P+S242H, E188P+S242W, E188P+S242P, E188P+S242I, E188P+S242T, E188P+S242L, E188P+K279W, E188P+K279Y, E188P+K279F, E188P+K279H, E188P+K279I, E188P+K279L, E188P+K279D, E188P+K279M, E188P+K279S, E188P+K279T E188P+K279N, E188P+K279Q, E188P+K279V, E188P+K279A, E188P+N275F, E188P+N275Y, E188P+N275W y E188P+N275H, donde la variante tiene al menos el 60 %, al menos el 65 %, al menos el 70 %, al menos el 75 %, al menos el 80 %, al menos el 85 %, al menos el 90 %, al menos el 95 %, al menos el 96 %, al menos el 97 %, al menos el 98 % o al menos el 99 %, pero menos del 100 % de identidad de secuencia con una alfa-amilasa precursora seleccionada del grupo que consiste en: SEC. ID NO.: 1, SEC. ID NO.: 2, SEC. ID NO.: 3, SEC. ID NO.: 4, SEC. ID NO.: 5, SEC. ID NO.: 6, SEC. ID NO.: 7, SEC. ID NO.: 8, SEC. ID NO.: 9, SEC. ID NO.: 10, SEC. ID NO.: 11, SEC. ID NO.: 12, SEC. ID NO.: 13, SEC. ID NO.: 14, SEC. ID NO.: 15, SEC. ID NO.: 16, SEC. ID NO.: 17, SEC. ID NO.: 18 y SEC. ID NO.: 27. La presente invención también se refiere a polinucleótidos que codifican las variantes; a construcciones de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN201741044156 | 2017-12-08 | ||
| IN201841031044 | 2018-08-20 | ||
| PCT/US2018/064413 WO2019113413A1 (en) | 2017-12-08 | 2018-12-07 | Alpha-amylase variants and polynucleotides encoding same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020005458A true MX2020005458A (es) | 2020-08-27 |
Family
ID=64746662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020005458A MX2020005458A (es) | 2017-12-08 | 2018-12-07 | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11326153B2 (es) |
| EP (1) | EP3720955B1 (es) |
| JP (2) | JP2021505167A (es) |
| CN (1) | CN111601887A (es) |
| BR (1) | BR112020011278A2 (es) |
| DK (1) | DK3720955T5 (es) |
| ES (1) | ES2955269T3 (es) |
| FI (1) | FI3720955T3 (es) |
| MX (1) | MX2020005458A (es) |
| WO (1) | WO2019113413A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113454214A (zh) * | 2019-03-21 | 2021-09-28 | 诺维信公司 | α-淀粉酶变体以及对其进行编码的多核苷酸 |
| EP3987022A1 (en) * | 2019-06-24 | 2022-04-27 | Novozymes A/S | Alpha-amylase variants |
| US20220325204A1 (en) * | 2019-08-27 | 2022-10-13 | Novozymes A/S | Detergent composition |
| AU2021370998A1 (en) * | 2020-11-02 | 2023-05-25 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| JP2023095355A (ja) * | 2021-12-24 | 2023-07-06 | 花王株式会社 | アミラーゼ配合洗浄剤組成物 |
| EP4495232A1 (en) | 2022-03-18 | 2025-01-22 | Kao Corporation | Alpha-amylase variant |
| CN114606216B (zh) * | 2022-03-31 | 2023-08-11 | 山东隆科特酶制剂有限公司 | 一种表达量提高的α-淀粉酶突变体Q441N/N442H及其编码基因和应用 |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
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| CN116286763B (zh) * | 2023-02-28 | 2024-06-25 | 西北工业大学 | 一种热稳定性提高的胱硫醚β-合成酶突变体及应用 |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
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-
2018
- 2018-12-07 WO PCT/US2018/064413 patent/WO2019113413A1/en not_active Ceased
- 2018-12-07 US US16/770,797 patent/US11326153B2/en active Active
- 2018-12-07 FI FIEP18822572.6T patent/FI3720955T3/fi active
- 2018-12-07 ES ES18822572T patent/ES2955269T3/es active Active
- 2018-12-07 EP EP18822572.6A patent/EP3720955B1/en active Active
- 2018-12-07 DK DK18822572.6T patent/DK3720955T5/da active
- 2018-12-07 JP JP2020531152A patent/JP2021505167A/ja active Pending
- 2018-12-07 CN CN201880077831.5A patent/CN111601887A/zh active Pending
- 2018-12-07 BR BR112020011278-0A patent/BR112020011278A2/pt unknown
- 2018-12-07 MX MX2020005458A patent/MX2020005458A/es unknown
-
2023
- 2023-11-09 JP JP2023191496A patent/JP2024026072A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FI3720955T3 (fi) | 2023-09-05 |
| DK3720955T5 (da) | 2024-08-26 |
| EP3720955A1 (en) | 2020-10-14 |
| JP2021505167A (ja) | 2021-02-18 |
| JP2024026072A (ja) | 2024-02-28 |
| CN111601887A (zh) | 2020-08-28 |
| US11326153B2 (en) | 2022-05-10 |
| CA3084211A1 (en) | 2019-06-13 |
| EP3720955B1 (en) | 2023-06-14 |
| ES2955269T3 (es) | 2023-11-29 |
| DK3720955T3 (da) | 2023-09-04 |
| US20210189364A1 (en) | 2021-06-24 |
| WO2019113413A1 (en) | 2019-06-13 |
| BR112020011278A2 (pt) | 2020-11-17 |
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