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MX2019009318A - Expresion de polipeptidos de nitrogenasa en celulas vegetales. - Google Patents

Expresion de polipeptidos de nitrogenasa en celulas vegetales.

Info

Publication number
MX2019009318A
MX2019009318A MX2019009318A MX2019009318A MX2019009318A MX 2019009318 A MX2019009318 A MX 2019009318A MX 2019009318 A MX2019009318 A MX 2019009318A MX 2019009318 A MX2019009318 A MX 2019009318A MX 2019009318 A MX2019009318 A MX 2019009318A
Authority
MX
Mexico
Prior art keywords
plant cells
fusions
expression
polypeptides
present disclosure
Prior art date
Application number
MX2019009318A
Other languages
English (en)
Inventor
Christopher Wood Craig
Silas Allen Robert
Okada Shoko
Charles Warden Andrew
Thelma Tilbrook Kimberley
Craig Taylor Matthew
Original Assignee
Commw Scient Ind Res Org
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2017900359A external-priority patent/AU2017900359A0/en
Application filed by Commw Scient Ind Res Org filed Critical Commw Scient Ind Res Org
Publication of MX2019009318A publication Critical patent/MX2019009318A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/07—Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/06—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with dinitrogen as acceptor (1.18.6)
    • C12Y118/06001—Nitrogenase (1.18.6.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cereal-Derived Products (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Seeds, Soups, And Other Foods (AREA)

Abstract

La presente invención se refiere a métodos y medios para producir polipéptidos de nitrogenasa en las mitocondrias de células vegetales. La presente descripción proporciona células vegetales que expresan una o más fusiones MTP-Nif y/o fusiones NifD-NifK y NifE-NifN traslacionales. La presente descripción también proporciona constructos de ácidos nucleicos que codifican estas fusiones, así como también constructos de expresión para expresar y dirigir las fusiones a las mitocondrias de células vegetales. La presente descripción también proporciona plantas transgénicas que comprenden las células vegetales de la invención y productos obtenidos de estas.
MX2019009318A 2017-02-06 2018-02-06 Expresion de polipeptidos de nitrogenasa en celulas vegetales. MX2019009318A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2017900359A AU2017900359A0 (en) 2017-02-06 Expression of nitrogenase polypeptides in plant cells
PCT/AU2018/050084 WO2018141030A1 (en) 2017-02-06 2018-02-06 Expression of nitrogenase polypeptides in plant cells

Publications (1)

Publication Number Publication Date
MX2019009318A true MX2019009318A (es) 2019-12-11

Family

ID=63039300

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019009318A MX2019009318A (es) 2017-02-06 2018-02-06 Expresion de polipeptidos de nitrogenasa en celulas vegetales.
MX2024000163A MX2024000163A (es) 2017-02-06 2019-08-05 Expresion de polipeptidos de nitrogenasa en celulas vegetales.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2024000163A MX2024000163A (es) 2017-02-06 2019-08-05 Expresion de polipeptidos de nitrogenasa en celulas vegetales.

Country Status (15)

Country Link
US (2) US11802290B2 (es)
EP (1) EP3577216A4 (es)
JP (3) JP7677753B2 (es)
KR (2) KR20190115064A (es)
CN (1) CN110475861A (es)
AU (2) AU2018216169B2 (es)
BR (1) BR112019016166A2 (es)
CA (1) CA3052215A1 (es)
IL (1) IL268466B2 (es)
MX (2) MX2019009318A (es)
MY (1) MY202283A (es)
PH (1) PH12019501787A1 (es)
UA (1) UA130051C2 (es)
WO (1) WO2018141030A1 (es)
ZA (1) ZA201905440B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018000729B1 (pt) 2015-07-13 2023-02-07 Pivot Bio, Inc Método de aumento de uma quantidade de nitrogênio atmosférico em uma planta de milho em um campo
CN114144527A (zh) * 2019-03-08 2022-03-04 联邦科学技术研究组织 固氮酶多肽在植物细胞中的表达
US20240117326A1 (en) * 2019-10-10 2024-04-11 Commonwealth Scientific And Industrial Research Organisation Expression of nitrogenase polypeptides in plant cells
CN110846270A (zh) * 2019-11-27 2020-02-28 北京市农林科学院 小麦原生质体的制备方法及小麦原生质体介导的转化方法
US20230257317A1 (en) * 2020-05-01 2023-08-17 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
CN114703233B (zh) * 2022-06-06 2022-09-13 广东省科学院生态环境与土壤研究所 一种提高梭菌发酵产氢效率的方法及其应用
CN119855908A (zh) * 2022-09-14 2025-04-18 孟山都技术公司 植物调控元件及其用途
CN116904338A (zh) * 2023-03-28 2023-10-20 福建农林大学 一株受氮调控的联合固氮菌及其应用
WO2024259585A1 (en) * 2023-06-20 2024-12-26 Peking University Method for co-expressing proteins
CN117844863B (zh) * 2024-03-06 2024-05-17 云南师范大学 一种马铃薯线粒体靶向表达载体、构建方法及应用
WO2025240869A1 (en) * 2024-05-16 2025-11-20 The Regents Of The University Of California N2-reduction by simplified nifen-based nitrogenase systems

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130305398A1 (en) * 2012-02-16 2013-11-14 Marie Coffin Genes and uses for plant enhacement
CN102690808B (zh) * 2011-03-23 2017-04-19 北京大学 为真核表达的目的构建原核基因表达岛
BR112015013058A2 (pt) * 2012-12-03 2017-09-12 Zaltsman Adi autofixação de nitrogênio em plantas por mimetização de vias procarióticas
EP2848684A1 (en) * 2013-09-11 2015-03-18 Universidad Politécnica de Madrid Reagents and methods for the expression of oxygen-sensitive proteins
US20170335294A1 (en) * 2014-05-09 2017-11-23 Nitrogenics LLC Plants capable of nitrogen fixation
US10913939B2 (en) * 2015-04-01 2021-02-09 Monsanto Technology Llc Compositions and methods for expression of nitrogenase in plant cells

Also Published As

Publication number Publication date
WO2018141030A1 (en) 2018-08-09
BR112019016166A2 (pt) 2020-03-24
IL268466A (en) 2019-09-26
JP2023052262A (ja) 2023-04-11
CA3052215A1 (en) 2018-08-09
JP2024167185A (ja) 2024-12-03
US20200095598A1 (en) 2020-03-26
RU2019127979A (ru) 2021-03-09
MX2024000163A (es) 2024-01-24
JP7677753B2 (ja) 2025-05-15
US20240360465A1 (en) 2024-10-31
US11802290B2 (en) 2023-10-31
CN110475861A (zh) 2019-11-19
IL268466B2 (en) 2025-07-01
KR20250051125A (ko) 2025-04-16
RU2019127979A3 (es) 2021-10-28
AU2018216169A1 (en) 2019-09-12
EP3577216A1 (en) 2019-12-11
IL268466B1 (en) 2025-03-01
JP2020510413A (ja) 2020-04-09
PH12019501787A1 (en) 2020-06-29
EP3577216A4 (en) 2020-10-28
ZA201905440B (en) 2021-01-27
KR20190115064A (ko) 2019-10-10
UA130051C2 (uk) 2025-10-29
AU2018216169B2 (en) 2024-07-18
AU2024227369A1 (en) 2024-11-07
MY202283A (en) 2024-04-22

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