MX2019000805A - Variantes de serinproteasa y polinucleotidos que las codifican. - Google Patents
Variantes de serinproteasa y polinucleotidos que las codifican.Info
- Publication number
- MX2019000805A MX2019000805A MX2019000805A MX2019000805A MX2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A
- Authority
- MX
- Mexico
- Prior art keywords
- variants
- protease
- polynucleotides encoding
- serine protease
- increase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Confectionery (AREA)
Abstract
La presente invención se refiere a variantes de proteasa, que presentan una mejora de las propiedades en comparación con la proteasa progenitora, en particular variantes de una serinproteasa que pertenece a la familia 53 obtenida a partir de una cepa de Meripilus giganteus. Las variantes de acuerdo con la invención presentan en particular un incremento de la termoestabilidad, por ejemplo, un incremento de la actividad residual después de 30 min a una temperatura en el intervalo de 55 a 65 °C y/o incremento de la temperatura de desnaturalización térmica, en comparación con la proteasa de Meripilus giganteus progenitora. La presente invención también se refiere a polinucleótidos que codifican las variantes; a constructos de ácido nucleico, vectores y células huésped que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16180497 | 2016-07-21 | ||
| EP16195078 | 2016-10-21 | ||
| PCT/EP2017/067883 WO2018015304A1 (en) | 2016-07-21 | 2017-07-14 | Serine protease variants and polynucleotides encoding same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019000805A true MX2019000805A (es) | 2019-06-20 |
Family
ID=59501396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019000805A MX2019000805A (es) | 2016-07-21 | 2017-07-14 | Variantes de serinproteasa y polinucleotidos que las codifican. |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US10927361B2 (es) |
| EP (1) | EP3487996B1 (es) |
| CN (1) | CN109642225A (es) |
| CA (1) | CA3028875A1 (es) |
| DK (1) | DK3487996T5 (es) |
| ES (1) | ES2981748T3 (es) |
| FI (1) | FI3487996T3 (es) |
| MX (1) | MX2019000805A (es) |
| WO (1) | WO2018015304A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018015303A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
| WO2018015304A1 (en) * | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
| US12359227B2 (en) * | 2019-03-18 | 2025-07-15 | Novozymes A/S | Polypeptides having pullulanase activity suitable for use in liquefaction |
| KR102316445B1 (ko) * | 2019-11-29 | 2021-10-26 | 씨제이제일제당 주식회사 | 신규 세린 프로테아제 변이체 |
| CN112574978B (zh) * | 2021-01-19 | 2021-08-17 | 青岛尚德生物技术有限公司 | 提高降解醇溶蛋白能力的蛋白酶突变体及其编码基因和应用 |
| PE20251535A1 (es) | 2022-05-14 | 2025-06-05 | Novozymes As | Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas |
| CN120693401A (zh) | 2022-12-19 | 2025-09-23 | 诺维信公司 | 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途 |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| EP4638768A2 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
| EP4638725A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
| US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
| US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
| JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| DE3781732T2 (de) | 1986-07-09 | 1993-03-25 | Novo Industri As | Mischungen von alpha-amylase zur verfluessigung von staerke. |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| JP3016587B2 (ja) | 1989-12-04 | 2000-03-06 | ザ・リサーチ・ファンデーション・オブ・ステート・ユニバーシティ・オブ・ニューヨーク | 非ステロイド抗炎症剤及びテトラサイクリンの配合 |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| FI964808A0 (fi) | 1994-06-03 | 1996-12-02 | Novo Nordisk Biotech Inc | Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot |
| JP3167729B2 (ja) | 1994-06-30 | 2001-05-21 | ノボ ノルディスク バイオテック，インコーポレイティド | 無毒、非毒素原性、非病原性の発現系、並びにその中で利用するためのプロモーター及びターミネーター |
| CA2305191C (en) | 1997-10-13 | 2011-09-27 | Novo Nordisk A/S | .alpha.-amylase mutants |
| CA2344619C (en) | 1998-10-26 | 2012-01-03 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
| AU4025700A (en) | 1999-03-22 | 2000-10-09 | Novo Nordisk Biotech, Inc. | Promoters for expressing genes in a fungal cell |
| AU2002319696B2 (en) | 2001-07-27 | 2007-11-01 | The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| DK1468093T4 (en) | 2002-01-23 | 2018-04-09 | Dsm Ip Assets Bv | Fermentation of pentose sugars |
| AU2003217338A1 (en) | 2002-02-08 | 2003-09-02 | Genencor International, Inc. | Methods for producing ethanol from carbon substrates |
| WO2004085627A1 (en) | 2003-03-26 | 2004-10-07 | Forskarpatent I Syd Ab | New saccharomyces cerevisiae strains utilizing xylose |
| US7968318B2 (en) | 2006-06-06 | 2011-06-28 | Genencor International, Inc. | Process for conversion of granular starch to ethanol |
| EP2250262B1 (en) | 2008-03-07 | 2015-07-29 | DSM IP Assets B.V. | A pentose sugar fermenting cell |
| CN102186982A (zh) * | 2008-08-20 | 2011-09-14 | 诺维信公司 | 生产发酵产物的方法 |
| EP2367928B1 (en) | 2008-12-24 | 2018-03-14 | DSM IP Assets B.V. | Xylose isomerase genes and their use in fermentation of pentose sugars |
| AU2010276468B2 (en) | 2009-11-30 | 2015-05-14 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| MX2012006176A (es) | 2009-12-01 | 2012-06-25 | Novozymes North America Inc | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. |
| CN102753680B (zh) | 2009-12-11 | 2015-05-13 | 诺维信公司 | 蛋白酶变体 |
| CN103189715B (zh) | 2010-11-05 | 2015-12-02 | 松下知识产权经营株式会社 | 立体图像处理装置及立体图像处理方法 |
| US9732332B2 (en) | 2010-11-08 | 2017-08-15 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| EP2527448A1 (en) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi |
| WO2013006756A2 (en) | 2011-07-06 | 2013-01-10 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
| EP2893012B1 (en) * | 2012-09-05 | 2018-08-15 | Novozymes A/S | Polypeptides having protease activity |
| BR112016003339B1 (pt) * | 2013-08-30 | 2023-02-14 | Novozymes A/S | Composição de enzima, e, processo de produção de um produto de fermentação a partir de material contendo amido |
| CN106455630B (zh) | 2014-06-27 | 2021-08-24 | 帝斯曼知识产权资产管理有限公司 | 改进动物饲料的营养价值的方法 |
| CA2995423A1 (en) | 2015-09-25 | 2017-03-30 | Novozymes A/S | Use of serine proteases for improving ethanol yield |
| WO2018015304A1 (en) * | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
| WO2018015303A1 (en) * | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
-
2017
- 2017-07-14 WO PCT/EP2017/067883 patent/WO2018015304A1/en not_active Ceased
- 2017-07-14 CN CN201780044163.1A patent/CN109642225A/zh active Pending
- 2017-07-14 DK DK17746005.2T patent/DK3487996T5/da active
- 2017-07-14 MX MX2019000805A patent/MX2019000805A/es unknown
- 2017-07-14 CA CA3028875A patent/CA3028875A1/en active Pending
- 2017-07-14 EP EP17746005.2A patent/EP3487996B1/en active Active
- 2017-07-14 FI FIEP17746005.2T patent/FI3487996T3/fi active
- 2017-07-14 US US16/318,496 patent/US10927361B2/en active Active
- 2017-07-14 ES ES17746005T patent/ES2981748T3/es active Active
-
2021
- 2021-01-14 US US17/148,790 patent/US11447763B2/en active Active
- 2021-12-09 US US17/547,098 patent/US12291730B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DK3487996T3 (da) | 2024-05-21 |
| EP3487996B1 (en) | 2024-04-10 |
| US12291730B2 (en) | 2025-05-06 |
| US10927361B2 (en) | 2021-02-23 |
| US20210139876A1 (en) | 2021-05-13 |
| US11447763B2 (en) | 2022-09-20 |
| US20220090041A1 (en) | 2022-03-24 |
| WO2018015304A1 (en) | 2018-01-25 |
| CN109642225A (zh) | 2019-04-16 |
| US20190352629A1 (en) | 2019-11-21 |
| ES2981748T3 (es) | 2024-10-10 |
| FI3487996T3 (fi) | 2024-07-10 |
| EP3487996A1 (en) | 2019-05-29 |
| CA3028875A1 (en) | 2018-01-25 |
| DK3487996T5 (da) | 2024-09-02 |
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