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MX2018013367A - Metodos para etiquetar bibliotecas codificadas por adn. - Google Patents

Metodos para etiquetar bibliotecas codificadas por adn.

Info

Publication number
MX2018013367A
MX2018013367A MX2018013367A MX2018013367A MX2018013367A MX 2018013367 A MX2018013367 A MX 2018013367A MX 2018013367 A MX2018013367 A MX 2018013367A MX 2018013367 A MX2018013367 A MX 2018013367A MX 2018013367 A MX2018013367 A MX 2018013367A
Authority
MX
Mexico
Prior art keywords
methods
encoded libraries
libraries
tagging dna
tagging
Prior art date
Application number
MX2018013367A
Other languages
English (en)
Inventor
Anthony D Keefe
Richard W Wagner
Alexander Litovchick
Matthew Clark
John W Cuozzo
Ying Zhang
Paolo A Centrella
Christopher D Hupp
Original Assignee
X Chem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47832597&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2018013367(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by X Chem Inc filed Critical X Chem Inc
Publication of MX2018013367A publication Critical patent/MX2018013367A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Structural Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

La presente invención se refiere a bibliotecas codificadas por oligonucleótidos y métodos de etiquetas tales bibliotecas. En particular, los métodos y oligonucleótidos pueden incluir uno o más nucleótidos 2'-sustituidos, tales como 2'-O-metil o 2'-fluoro nucleótidos, y otras condiciones u otros reactivos para crecentar la ligación enzimática de una o más funcionalidades químicas para dar soporte a la ligación química.
MX2018013367A 2011-09-07 2014-03-07 Metodos para etiquetar bibliotecas codificadas por adn. MX2018013367A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161531820P 2011-09-07 2011-09-07
US201161536929P 2011-09-20 2011-09-20

Publications (1)

Publication Number Publication Date
MX2018013367A true MX2018013367A (es) 2021-11-16

Family

ID=47832597

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014002854A MX360438B (es) 2011-09-07 2012-09-07 Metodos para etiquetar bibliotecas codificadas por adn.
MX2018013367A MX2018013367A (es) 2011-09-07 2014-03-07 Metodos para etiquetar bibliotecas codificadas por adn.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014002854A MX360438B (es) 2011-09-07 2012-09-07 Metodos para etiquetar bibliotecas codificadas por adn.

Country Status (17)

Country Link
US (2) US10865409B2 (es)
EP (3) EP3828271A1 (es)
JP (3) JP6674738B2 (es)
KR (2) KR102124613B1 (es)
CN (1) CN103998658B (es)
AP (1) AP2014007483A0 (es)
AU (3) AU2012304387B2 (es)
BR (1) BR112014005205A2 (es)
CA (2) CA3077662A1 (es)
DK (2) DK2748357T3 (es)
EA (1) EA032438B1 (es)
ES (2) ES2852073T3 (es)
IL (2) IL231191B (es)
IN (1) IN2014CN02574A (es)
MX (2) MX360438B (es)
SG (2) SG10201605812YA (es)
WO (1) WO2013036810A1 (es)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2518626A1 (en) * 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
CA3077662A1 (en) 2011-09-07 2013-03-14 X-Chem, Inc. Methods for tagging dna-encoded libraries
ES2855137T3 (es) * 2012-07-13 2021-09-23 X Chem Inc Bibliotecas codificadas por ADN que tienen vínculos de oligonucleótidos codificadores que no se pueden leer por polimerasas
US10156559B2 (en) 2012-12-10 2018-12-18 Fred Hutchinson Cancer Research Center Lipocalin fusion partners
CN103882532B (zh) * 2012-12-20 2015-10-21 成都先导药物开发有限公司 一种先导化合物的合成及筛选方法与试剂盒
WO2014100069A1 (en) * 2012-12-20 2014-06-26 Merck Sharp & Dohme Corp. POST-SYNTHETIC ORTHOGONAL AMIDATION PLUS METAL CATALYZED AZIDE-ALKYNE CYCLOADDITION CLICK CHEMISTRY ON siRNA
GB201322692D0 (en) 2013-12-20 2014-02-05 Philochem Ag Production of encoded chemical libraries
GB201404552D0 (en) * 2014-03-14 2014-04-30 Philochem Ag Purification of dna-conjugate products
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
MA41298A (fr) * 2014-12-30 2017-11-07 X Chem Inc Procédés de marquage de banques codées par de l'adn
CN104894651B (zh) * 2015-06-29 2017-04-12 天津诺禾医学检验所有限公司 微量起始dna的高通量测序文库构建方法及其所构建的高通量测序文库
WO2017218293A1 (en) * 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
CN107130300A (zh) * 2016-10-08 2017-09-05 深圳劲宇生物科技有限公司 一种dna编码分子库的合成方法及dna模板
US11359227B2 (en) * 2016-12-09 2022-06-14 University Of Southern California Genome-wide mapping of DNA-DNA proximities in the nucleus
EP3360962A1 (en) * 2017-02-14 2018-08-15 Technische Universität Dortmund Synthesis of dna-encoded libraries by micellar catalysis
JP6911248B2 (ja) 2017-03-17 2021-07-28 成都先導薬物開発股▲フン▼有限公司Ｈｉｔｇｅｎ Ｉｎｃ． エンコードライブラリの合成方法及び組成物
WO2018204420A1 (en) * 2017-05-02 2018-11-08 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
CA3068446A1 (en) * 2017-06-30 2019-01-02 The Regents Of The University Of California Methods and systems for evaluating dna methylation in cell-free dna
CN111295444B (zh) * 2017-09-25 2024-08-09 哈斯达克科学公司 用于寡核苷酸指导的组合化学的多项式编码
CN108070009B (zh) * 2017-12-12 2021-04-13 上海药明康德新药开发有限公司 一种制备dna编码化合物文库的方法及起始头片段化合物和制得的dna编码化合物
EP3737755B1 (en) * 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
WO2019149198A1 (zh) * 2018-01-31 2019-08-08 成都先导药物开发股份有限公司 一种dna编码化合物库及化合物筛选方法
WO2019157693A1 (zh) * 2018-02-14 2019-08-22 深圳劲宇生物科技有限公司 Dna编码分子库及应用广的化合物筛选方法
WO2019157692A1 (zh) * 2018-02-14 2019-08-22 深圳劲宇生物科技有限公司 Dna 编码分子库及无靶点限制的化合物筛选方法
CN120905352A (zh) 2018-04-02 2025-11-07 埃努梅拉分子股份有限公司 用于对核酸分子进行计数的方法、系统和组合物
CN110658163A (zh) * 2018-06-29 2020-01-07 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
CN109468310B (zh) * 2018-10-25 2020-12-01 深圳劲宇生物科技有限公司 Dna编码碎片分子库的合成方法和连接基团的筛选方法
CA3120895C (en) 2018-12-20 2024-07-02 Sergey N. Krylov BINDER SELECTION USING CAPILLARY ELECTROPHORESIS
CN120648764A (zh) * 2019-02-18 2025-09-16 味之素株式会社 包含互补部分的修饰寡核苷酸的制造方法
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
KR20210148304A (ko) * 2019-04-05 2021-12-07 클라렛 바이오사이언스, 엘엘씨 핵산을 분석하기 위한 방법 및 조성물
US20200392485A1 (en) * 2019-05-09 2020-12-17 Pacific Biosciences Of California, Inc. COMPOSITIONS AND METHODS FOR IMPROVED cDNA SYNTHESIS
US20220275362A1 (en) * 2019-07-25 2022-09-01 X-Chem, Inc. Methods for tagging and encoding of pre-existing compound libraries
EP4423103A4 (en) * 2021-10-27 2025-12-10 Juan Pablo Maianti DNA-encoded affinity-labeled, masked payload compounds and their use in assembling small-molecule libraries activated for late-stage purification and multiplex screening of covalent ligands
EP4594520A2 (en) 2022-09-30 2025-08-06 Sixfold Bioscience Ltd. Compositions containing oligonucleotides with theranostic applications
WO2025042899A2 (en) * 2023-08-24 2025-02-27 X-Chem, Inc. Methods for identifying compounds binding to a target molecule using mirror-imaging of the target molecule
KR102774172B1 (ko) * 2023-10-25 2025-02-28 서울대학교산학협력단 Rna 변형의 모든 주변 서열을 포함하는 광범위한 rna 변형 라이브러리 설계법 및 그 이용
WO2025217391A1 (en) * 2024-04-12 2025-10-16 Insitro, Inc. Methods of preparing oligonucleotide-directed combinatorial libraries

Family Cites Families (161)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0260032B1 (en) * 1986-09-08 1994-01-26 Ajinomoto Co., Inc. Compounds for the cleavage at a specific position of RNA, oligomers employed for the formation of said compounds, and starting materials for the synthesis of said oligomers
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5025388A (en) 1988-08-26 1991-06-18 Cramer Richard D Iii Comparative molecular field analysis (CoMFA)
US6005087A (en) 1995-06-06 1999-12-21 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5670633A (en) 1990-01-11 1997-09-23 Isis Pharmaceuticals, Inc. Sugar modified oligonucleotides that detect and modulate gene expression
US5723289A (en) 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
JP2780572B2 (ja) 1991-09-13 1998-07-30 株式会社島津製作所 オリゴヌクレオチドの酵素的合成法及びオリゴヌクレオチドのプライマーとしての使用
CA2118806A1 (en) 1991-09-18 1993-04-01 William J. Dower Method of synthesizing diverse collections of oligomers
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5633360A (en) 1992-04-14 1997-05-27 Gilead Sciences, Inc. Oligonucleotide analogs capable of passive cell membrane permeation
JPH08506175A (ja) 1992-10-01 1996-07-02 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク タグでコードされる複合体の組合せ化学ライブラリー
US6503759B1 (en) 1992-10-01 2003-01-07 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5807683A (en) 1992-11-19 1998-09-15 Combichem, Inc. Combinatorial libraries and methods for their use
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
AU684279B2 (en) 1993-04-12 1997-12-11 Northwestern University Method of forming oligonucleotides
CA2163637C (en) 1993-05-27 2008-05-06 Michal Lebl Topologically segregated, encoded solid phase libraries
US5840485A (en) 1993-05-27 1998-11-24 Selectide Corporation Topologically segregated, encoded solid phase libraries
AU7323194A (en) 1993-07-02 1995-01-24 Lynx Therapeutics, Inc. Synthesis of branched nucleic acids
US5571903A (en) 1993-07-09 1996-11-05 Lynx Therapeutics, Inc. Auto-ligating oligonucleotide compounds
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
AU7390994A (en) 1993-08-25 1995-03-21 Symbicom Aktiebolag Molecular modelling and drug design
US5658782A (en) 1993-10-20 1997-08-19 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University A Non-Profit Organization Amino acid transporters and uses
GB2298863B (en) 1993-11-02 1998-03-11 Affymax Tech Nv Apparatus and process for the synthesis of diverse compounds especially for generating and screening compound libraries
US6117976A (en) 1993-11-04 2000-09-12 Medical Research Council Manufacture and use of polypeptides tagged using binding molecules
EP0736103A4 (en) 1993-12-17 1999-07-28 Roger S Cubicciotti NUCLEOTIDE-DIRECTED ASSEMBLY OF MEDICAMENTS AND BIMOLECULAR AND MULTIMOLECULAR SYSTEMS
US6936477B2 (en) 1994-04-13 2005-08-30 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
JPH08268A (ja) 1994-06-16 1996-01-09 Koichi Nishigaki 連結した２本鎖ｄｎａの製造方法
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5525734A (en) 1994-06-22 1996-06-11 Affymax Technologies N.V. Methods for synthesizing diverse collections of pyrrolidine compounds
NZ291554A (en) 1994-07-26 1999-02-25 Scripps Research Inst Production of soluble polymer-linked peptide/nucleic acid libraries
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
WO1996038726A1 (en) 1995-05-30 1996-12-05 Ecole Polytechnique Federale De Lausanne (Epfl) Covalently immobilized phospholipid bilayers on solid surfaces
US5780613A (en) 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5962228A (en) 1995-11-17 1999-10-05 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5846839A (en) 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5898031A (en) 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
DE19642751A1 (de) 1996-10-16 1998-04-23 Deutsches Krebsforsch Saccharid-Bibliothek
RU2233878C2 (ru) 1997-01-21 2004-08-10 Дзе Дженерал Хоспитал Корпорейшн Способ отбора желательного белка и нуклеиновой кислоты, средства для его осуществления
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US20020034732A1 (en) 1997-07-30 2002-03-21 Daniel J. Capon Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening
CA2302292A1 (en) 1997-08-29 1999-03-04 Selective Genetics, Inc. Methods using phage display for selecting internalizing ligands for gene delivery
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
ATE215604T1 (de) * 1998-06-22 2002-04-15 Larova Biochemie Gmbh Herstellung von polymeren aus nukleotidischen und/oder nicht-nukleotidischen bausteinen auf enzymatischem weg
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
AU6502599A (en) 1998-10-05 2000-04-26 Lynx Therapeutics, Inc. Enzymatic synthesis of oligonucleotide tags
US7479472B1 (en) 1998-10-19 2009-01-20 The Board Of Trustees Of The Leland Stanford Junior University DNA-templated combinatorial library chemistry
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
CA2350417C (en) 1998-12-02 2010-02-09 Phylos, Inc. Dna-protein fusions and uses thereof
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
CA2403209A1 (en) 1999-04-08 2000-10-19 Pavel V. Sergeev Synthesis of biologically active compounds in cells
EP1177281B1 (en) * 1999-04-30 2009-01-21 Cyclops Genome Sciences Limited Isolation of nucleic acid
US7270969B2 (en) 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
EP1212452B1 (en) 1999-08-27 2013-07-17 Bristol-Myers Squibb Company Methods for encoding and sorting in vitro translated proteins
DE60034954D1 (de) 1999-09-14 2007-07-05 Xenoport Inc Substrate und screeningverfahren für transportproteine
EP1218544B1 (en) 1999-10-04 2009-06-03 The University of Medicine and Dentistry of New Jersey TAR RNA binding peptides
US6844324B1 (en) 1999-11-12 2005-01-18 Massachusetts Institute Of Technology Modular peptide mediated intracellular delivery system and uses therefore
AU2001241668A1 (en) 2000-02-23 2001-09-03 Xenoport, Inc. Self-encoded combinatorial synthesis of compound multiplets
US6936467B2 (en) 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US20020072887A1 (en) 2000-08-18 2002-06-13 Sandor Szalma Interaction fingerprint annotations from protein structure models
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
EP1331572B1 (en) 2000-09-20 2012-08-08 ARKRAY, Inc. Client support system
US6706481B2 (en) 2000-10-27 2004-03-16 Research Development Foundation In vitro selection of signaling aptamers
JP2002315577A (ja) 2000-11-14 2002-10-29 Gencom Co 核酸ライブラリーの作製方法
US20030049616A1 (en) 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
ES2271306T5 (es) 2001-03-19 2013-10-16 President And Fellows Of Harvard College Evolución de una nueva función molecular
US20060234231A1 (en) 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
WO2004001042A2 (en) 2002-06-20 2003-12-31 Nuevolution A/S Microarrays displaying encoded molecules
US20030143561A1 (en) 2001-06-20 2003-07-31 Nuevolution A/S Nucleoside derivatives for library preparation
NZ530098A (en) 2001-06-20 2006-02-24 Nuevolution As Templated molecules and methods for using such molecules
EP1401850A1 (en) 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
AU2002310846B2 (en) 2002-03-08 2008-03-20 Eidgenossische Technische Hochschule Zurich Encoded self-assembling chemical libraries (ESACHEL)
AU2003218629A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
AU2003214032A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c=c double bond upon reaction
US20050221318A1 (en) 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
AU2003218630A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
CA2518626A1 (en) 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
US7749699B2 (en) 2002-07-18 2010-07-06 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
WO2004009814A1 (en) 2002-07-23 2004-01-29 Nuevolution A/S Gene shuffling by template switching
EP1539980B1 (en) 2002-08-01 2016-02-17 Nuevolution A/S Library of complexes comprising small non-peptide molecules and double-stranded oligonucleotides identifying the molecules
WO2004016767A2 (en) 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
WO2004024929A2 (en) 2002-09-12 2004-03-25 Nuevolution A/S Proximity-aided synthesis of templated molecules
DK2348124T3 (en) * 2002-10-30 2014-03-10 Nuevolution As Synthesis of a bifunctional complex
EP1756277B1 (en) 2002-12-19 2009-12-02 Nuevolution A/S Quasirandom structure and function guided synthesis methods
WO2004074429A2 (en) 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
EP1597394A2 (en) 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
DK1608748T3 (da) 2003-03-20 2009-06-29 Nuevolution As Ligeringskodning af små molekyler
EP1652123A2 (en) 2003-07-03 2006-05-03 Biogen Idec MA Inc. STRUCTURAL INTERACTION FINGERPRINT (SIFt)
EP1668157A4 (en) 2003-07-07 2007-10-24 One Cell Systems Inc HAIRPIN MARKED PROBES AND METHOD OF USE
EP1533385A1 (en) 2003-09-05 2005-05-25 Nuevolution A/S Templated compounds for generation of encoded molecules and directional methods using such compounds
US7365179B2 (en) 2003-09-09 2008-04-29 Compass Genetics, Llc Multiplexed analytical platform
DE602004023960D1 (de) 2003-09-18 2009-12-17 Nuevolution As Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen
WO2005050224A2 (en) 2003-11-13 2005-06-02 Epitome Biosystems Inc. Small molecule and peptide arrays and uses thereof
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
WO2005058479A2 (en) 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US20050153321A1 (en) 2003-12-30 2005-07-14 Saba James A. Libraries of multiple-ligand-conjugated nucleic acids
EP1713936B1 (en) 2004-02-12 2009-12-09 Population Genetics Technologies Ltd Corporation of Great Britain Genetic analysis by sequence-specific sorting
EP1723255B1 (en) 2004-02-17 2010-12-29 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
DK1730277T3 (da) 2004-03-22 2010-03-01 Nuevolution As Kodning ved ligering under anvendelse af byggebloksoligonukleotider
US7405287B2 (en) 2004-08-03 2008-07-29 Board Of Regents, The University Of Texas System Method of treating a cancer
US7745614B2 (en) 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
US20060099626A1 (en) 2004-10-27 2006-05-11 Harbury Pehr B DNA-templated combinatorial library device and method for use
KR101411361B1 (ko) 2004-11-08 2014-06-25 비퍼겐 에이피에스 구조적 핵산에 의해 가이드되는 화학적 합성
US8168381B2 (en) 2004-11-22 2012-05-01 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries
AU2005316503A1 (en) 2004-12-17 2006-06-22 Dynavax Technologies Corporation Methods and compositions for induction or promotion of immune tolerance
CN101090979A (zh) * 2004-12-22 2007-12-19 埃克斯魁恩公司 用于分析核酸混合物的探针、文库和试剂盒及其构建方法
AU2006206219B2 (en) * 2005-01-21 2012-07-26 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US20060211030A1 (en) 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
DK1910538T3 (da) * 2005-06-09 2011-08-08 Praecis Pharm Inc Fremgangsmåder til syntese af kodede biblioteker
WO2006138560A2 (en) 2005-06-17 2006-12-28 President And Fellows Of Harvard College Nucleic acid-templated chemistry in organic solvents
WO2007014267A2 (en) 2005-07-26 2007-02-01 Oregon Health & Science University Nanoparticle probes for capture, sorting and placement of targets
WO2007016488A2 (en) 2005-07-29 2007-02-08 Ensemble Discovery Corporation Analysis of encoded chemical libraries
EP2458011B1 (en) 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
AU2006309096B2 (en) 2005-10-28 2013-07-04 Glaxosmithkline Llc Methods for identifying compounds of interest using encoded libraries
DK1957644T3 (da) 2005-12-01 2011-03-28 Nuevolution As Enzymatiske kodnings metoder til effektiv syntese af store biblioteker
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007124758A1 (en) 2006-05-03 2007-11-08 Vipergen Aps A method for preparing compounds by nucleic acid directed synthesis
CN101528673B (zh) 2006-10-26 2012-08-01 住友化学株式会社 不对称铜络合物结晶的制造方法
CN101219219B (zh) 2007-01-10 2013-02-13 北京普罗吉生物科技发展有限公司 包含血管抑素或其片段的复合物、其制备方法及应用
ES2395240T3 (es) 2007-10-22 2013-02-11 Lgc Limited Oligonucleótidos y usos de los mismos
JP4940311B2 (ja) 2007-11-19 2012-05-30 国立大学法人北陸先端科学技術大学院大学 光クロスリンク能を有する光応答性人工ヌクレオチド
WO2009077173A2 (en) * 2007-12-19 2009-06-25 Philochem Ag Dna-encoded chemical libraries
US20120107840A1 (en) 2008-04-09 2012-05-03 Sru Biosystems, Inc Methods for Label Free Testing of Cells
JP4814904B2 (ja) 2008-04-16 2011-11-16 国立大学法人北陸先端科学技術大学院大学 核酸類の配列選択的な精製方法
EP2283132B1 (en) * 2008-05-02 2016-10-26 Epicentre Technologies Corporation Selective 5' ligation tagging of rna
TW201011006A (en) 2008-06-16 2010-03-16 Nuevolution As IAP binding compounds
ES2752025T3 (es) 2008-07-25 2020-04-02 Wagner Richard W Métodos de cribado de proteínas
US9222082B2 (en) 2009-01-30 2015-12-29 Oxford Nanopore Technologies Limited Hybridization linkers
AU2010213497A1 (en) 2009-02-13 2011-08-25 X-Body, Inc. Identification of nucleic acid delivery vehicles using DNA display
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
AU2010213510B2 (en) 2009-02-16 2014-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded DNA
US8728725B2 (en) 2009-07-06 2014-05-20 Trilink Biotechnologies Chemically modified ligase cofactors, donors and acceptors
WO2011056866A2 (en) * 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
DE102009058769A1 (de) 2009-12-16 2011-06-22 MagForce Nanotechnologies AG, 10589 Temperaturabhängige Aktivierung von katalytischen Nukleinsäuren zur kontrollierten Wirkstofffreisetzung
WO2011120042A1 (en) 2010-03-26 2011-09-29 Sru Biosystems, Inc. Use of induced pluripotent cells and other cells for screening compound libraries
EP3540059A1 (en) 2010-04-16 2019-09-18 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
EP3798236B1 (en) 2011-03-15 2022-08-10 X-Body, Inc. Antibody screening methods
US8846883B2 (en) 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
CA3077662A1 (en) 2011-09-07 2013-03-14 X-Chem, Inc. Methods for tagging dna-encoded libraries
ES2855137T3 (es) 2012-07-13 2021-09-23 X Chem Inc Bibliotecas codificadas por ADN que tienen vínculos de oligonucleótidos codificadores que no se pueden leer por polimerasas
GB201322692D0 (en) 2013-12-20 2014-02-05 Philochem Ag Production of encoded chemical libraries

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