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MX2018011276A - Regulación del sistema de señalizacion ras/camp/pka en levaduras. - Google Patents

Regulación del sistema de señalizacion ras/camp/pka en levaduras.

Info

Publication number
MX2018011276A
MX2018011276A MX2018011276A MX2018011276A MX2018011276A MX 2018011276 A MX2018011276 A MX 2018011276A MX 2018011276 A MX2018011276 A MX 2018011276A MX 2018011276 A MX2018011276 A MX 2018011276A MX 2018011276 A MX2018011276 A MX 2018011276A
Authority
MX
Mexico
Prior art keywords
yeast cells
mutated
ras
camp
protein
Prior art date
Application number
MX2018011276A
Other languages
English (en)
Inventor
Argyros Aaron
Froehlich Allan
HENNINGSEN Brooks
F Rice Charles
Oeser Michelle
Fisher Janet
M Zelle Rintze
Original Assignee
Lallemand Hungary Liquidity Man Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Man Llc filed Critical Lallemand Hungary Liquidity Man Llc
Publication of MX2018011276A publication Critical patent/MX2018011276A/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La descripción actual se refiere a la modulación en el sistema de señalización RAS/cAMP/PKA para mantener la eficiencia de propagación e incrementar eficiencia de fermentación de células de levadura. La descripción actual proporciona células de levadura que tienen se producen por ingeniería para exhibir una modulación en la señalización en un sistema RAS/cAMP/PKA, dependiendo de las condiciones. Por ejemplo las células de levadura pueden seleccionarse o modificarse genéticamente para expresar una proteína Rasl mutada, una proteína Ras2 mutada, una proteína Ira1 mutada y/o una proteina Ira2 mutada, opcionalmente en combinación con promotores específicos. También se proporcionan en la presente métodos para propagar las células de levadura así como el uso de las células de levadura para generar un producto fermentado (tal como etanol).
MX2018011276A 2016-03-18 2017-03-17 Regulación del sistema de señalizacion ras/camp/pka en levaduras. MX2018011276A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662310251P 2016-03-18 2016-03-18
PCT/EP2017/056456 WO2017158189A1 (en) 2016-03-18 2017-03-17 Regulation of the ras/camp/pka signaling pathway in yeasts

Publications (1)

Publication Number Publication Date
MX2018011276A true MX2018011276A (es) 2019-07-04

Family

ID=58358617

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018011276A MX2018011276A (es) 2016-03-18 2017-03-17 Regulación del sistema de señalizacion ras/camp/pka en levaduras.

Country Status (7)

Country Link
US (1) US11667680B2 (es)
EP (1) EP3430029A1 (es)
CN (1) CN109071617A (es)
BR (1) BR112018068981A2 (es)
CA (1) CA3018013A1 (es)
MX (1) MX2018011276A (es)
WO (1) WO2017158189A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11667886B2 (en) * 2017-06-13 2023-06-06 Dsm Ip Assets B.V. Recombinant yeast cell
US11739338B2 (en) * 2017-09-19 2023-08-29 Lallemand Hungary Liquidity Management Llc Acetate toxicity tolerance in recombinant microbial host cells
US11326174B2 (en) * 2018-08-17 2022-05-10 Wisconsin Alumni Research Foundation Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth
EP3853245A4 (en) 2018-09-19 2022-06-15 Lallemand USA, Inc. OPTIMIZATION OF YEAST HOST CELLS FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS
US11118017B2 (en) 2019-11-13 2021-09-14 American Process International LLC Process for the production of bioproducts from lignocellulosic material
US11306113B2 (en) 2019-11-13 2022-04-19 American Process International LLC Process for the production of cellulose, lignocellulosic sugars, lignosulfonate, and ethanol
CN110904087B (zh) * 2019-12-28 2021-05-11 浙江工业大学 L-阿拉伯糖差向异构酶突变体及其应用
CN113817662B (zh) * 2021-10-08 2023-08-29 徐州工程学院 用于提高微生物法制备3-羟基丙酸产率的诱导剂

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
AUPS334602A0 (en) 2002-06-28 2002-07-25 Unisearch Limited Glutathione production
JP5553433B2 (ja) 2006-10-02 2014-07-16 ディーエスエム アイピー アセッツ ビー．ブイ． アラビノース発酵性酵母細胞の代謝工学
DE102007016534B4 (de) 2007-04-05 2019-03-28 Butalco Gmbh Neuer Expressionsvektor von Codon-optimierten Genen eines Arabinose-Stoffwechselweges zur Arabinose-Umsetzung in Hefe
EP2171038A2 (en) 2007-07-19 2010-04-07 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
DE102008031350B4 (de) 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
EP3168300A1 (en) 2010-06-03 2017-05-17 Lallemand Hungary Liquidity Management LLC Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
US9598689B2 (en) 2011-11-10 2017-03-21 Lallemand Hungary Liquidity Management Llc Genetically modified strain of S. cerevisiae engineered to ferment xylose and arabinose
US9920312B2 (en) * 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization

Also Published As

Publication number Publication date
WO2017158189A1 (en) 2017-09-21
US11667680B2 (en) 2023-06-06
CA3018013A1 (en) 2017-09-21
BR112018068981A2 (pt) 2019-04-16
EP3430029A1 (en) 2019-01-23
CN109071617A (zh) 2018-12-21
US20190106464A1 (en) 2019-04-11

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