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MX2018005692A - Limitación de trehalosa producida por levaduras en fermentación. - Google Patents

Limitación de trehalosa producida por levaduras en fermentación.

Info

Publication number
MX2018005692A
MX2018005692A MX2018005692A MX2018005692A MX2018005692A MX 2018005692 A MX2018005692 A MX 2018005692A MX 2018005692 A MX2018005692 A MX 2018005692A MX 2018005692 A MX2018005692 A MX 2018005692A MX 2018005692 A MX2018005692 A MX 2018005692A
Authority
MX
Mexico
Prior art keywords
production
trehalose
fermentation
allowing
reducing
Prior art date
Application number
MX2018005692A
Other languages
English (en)
Inventor
Argyros Aaron
F Rice Charles
Skinner Ryan
Barrett Trisha
Original Assignee
Lallemand Hungary Liquidity Man Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Man Llc filed Critical Lallemand Hungary Liquidity Man Llc
Publication of MX2018005692A publication Critical patent/MX2018005692A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01028—Alpha,alpha-trehalase (3.2.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente descripción hace referencia a células hospedadoras de levadura recombinantes que presentan (i) una primera modificación genética para reducir la producción de una o más enzimas de origen natural que sirven para producir glicerol o que regulan la síntesis de glicerol y/o hacen posible la producción de una glucoamilasa heteróloga, y (ii) una segunda modificación genética para reducir la producción de una o más enzimas de origen natural que sirven para producir trehalosa o que regulan la síntesis de trehalosa y/o hacen posible la expresión de una trehalasa heteróloga. Las células hospedadoras de levadura recombinante se pueden utilizar para limitar la producción de trehalosa (en particular la trehalosa extracelular, producidas con levadura) durante la fermentación y, en alguna modalidades, pueden aumentar la producción de un producto de fermentación (tal como, por ejemplo, etanol).
MX2018005692A 2015-11-06 2016-11-04 Limitación de trehalosa producida por levaduras en fermentación. MX2018005692A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562251885P 2015-11-06 2015-11-06
PCT/IB2016/056658 WO2017077504A1 (en) 2015-11-06 2016-11-04 Limiting yeast-produced trehalose in fermentation

Publications (1)

Publication Number Publication Date
MX2018005692A true MX2018005692A (es) 2019-03-14

Family

ID=57321370

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018005692A MX2018005692A (es) 2015-11-06 2016-11-04 Limitación de trehalosa producida por levaduras en fermentación.

Country Status (6)

Country Link
US (6) US10570421B2 (es)
EP (1) EP3371317A1 (es)
CN (1) CN108474010A (es)
CA (1) CA3004228A1 (es)
MX (1) MX2018005692A (es)
WO (1) WO2017077504A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2018005692A (es) * 2015-11-06 2019-03-14 Lallemand Hungary Liquidity Man Llc Limitación de trehalosa producida por levaduras en fermentación.
CA3062324A1 (en) * 2017-05-04 2018-11-08 Cargill, Incorporated Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts
WO2019063542A1 (en) * 2017-09-29 2019-04-04 Dsm Ip Assets B.V. IMPROVED ETHANOL PRODUCTION WITHOUT GLYCEROL
US11866751B2 (en) 2018-07-25 2024-01-09 Novozymes A/S Yeast expressing a heterologous alpha-amylase for ethanol production
CN109182301B (zh) * 2018-08-17 2021-12-07 广东溢多利生物科技股份有限公司 一种二糖降解酶基因及其应用
CN112714794A (zh) * 2018-09-17 2021-04-27 南京百斯杰生物工程有限公司 海藻糖酶在发酵生产中的应用
CA3114783A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
BR112021009161A2 (pt) * 2018-11-13 2021-08-31 Lallemand Hungary Liquidity Management Llc. Expressão combinada de enzimas de produção de trealose e degradação de trealose
BR112021009163A2 (pt) * 2018-11-13 2021-08-17 Lallemand Hungary Liquidity Management Llc. combinações sinergísticas de bactéria e levedura
EP3744853A1 (en) * 2019-05-29 2020-12-02 Ohly GmbH Trehalose-rich yeast hydrolysate
BR112021026477A2 (pt) 2019-08-06 2022-02-08 Novozymes As Célula hospedeira recombinante, métodos de produção de um produto de fermentação a partir de um material contendo amido ou contendo celulose, de produção do polipeptídeo maduro, de produção de um derivado de uma célula hospedeira recombinante e de produção de etanol, construto de ácido nucleico ou vetor de expressão, composição, e, uso de uma célula hospedeira recombinante
WO2021104672A1 (en) 2019-11-29 2021-06-03 Lallemand Hungary Liquidity Management Llc Yeast expressing heterologous glucoamylase
BR112022010438A2 (pt) 2019-11-29 2022-10-11 Lallemand Hungary Liquidity Man Llc Processo para deslocamento de uma enzima exógena
BR112022022797A2 (pt) * 2020-05-11 2022-12-13 Lallemand Hungary Liquidity Man Llc Célula hospedeira de levedura recombinante que expressa uma hidrolase
MX2023014545A (es) 2021-06-07 2024-03-01 Novozymes As Microorganismo diseñado para mejorar la fermentación del etanol.
WO2023079048A1 (en) * 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
CN114350726A (zh) * 2022-01-11 2022-04-15 华南理工大学 一种以纤维素为底物合成海藻糖的方法
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
CN117568197B (zh) * 2023-11-20 2024-07-12 湖南农业大学 一种生产前列腺素F2α的酵母工程菌及其应用
WO2025133860A1 (en) 2023-12-21 2025-06-26 Danstar Ferment Ag Increased production of acetyl-phosphate and products derived therefrom in yeasts

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101914462B (zh) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程
CN102869770B (zh) * 2009-11-30 2015-12-16 诺维信公司 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸
EP3168300A1 (en) 2010-06-03 2017-05-17 Lallemand Hungary Liquidity Management LLC Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
US9732332B2 (en) * 2010-11-08 2017-08-15 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
DK2694662T3 (da) 2011-04-05 2020-03-02 Lallemand Hungary Liquidity Man Llc Fremgangsmåder til forbedring af produktudbytte og fremstilling i en mikroorganisme gennem addition af alternerende elektronacceptorer
WO2015023989A1 (en) 2013-08-15 2015-02-19 Lallemand Hungary Liquidity Management Llc Methods for the improvement of product yield and production in a microorganism through glycerol recycling
BR112016009365A2 (pt) 2013-10-28 2017-09-19 Danisco Us Inc Método para aumentar, melhorar e estender a produção de etanol e método para diminuir as concentrações finais de dp2 em um produto de fermentação
CN105722969A (zh) 2013-10-28 2016-06-29 丹尼斯科美国公司 大规模基因工程化活性干酵母
WO2015148272A1 (en) * 2014-03-28 2015-10-01 Danisco Us Inc. Altered host cell pathway for improved ethanol production
MX2018005692A (es) * 2015-11-06 2019-03-14 Lallemand Hungary Liquidity Man Llc Limitación de trehalosa producida por levaduras en fermentación.
WO2021104672A1 (en) * 2019-11-29 2021-06-03 Lallemand Hungary Liquidity Management Llc Yeast expressing heterologous glucoamylase

Also Published As

Publication number Publication date
US20210163999A1 (en) 2021-06-03
US20250179537A1 (en) 2025-06-05
US11634734B2 (en) 2023-04-25
BR112018009161A2 (pt) 2019-04-16
US20230265463A1 (en) 2023-08-24
EP3371317A1 (en) 2018-09-12
US10947568B2 (en) 2021-03-16
US20200231991A1 (en) 2020-07-23
CA3004228A1 (en) 2017-05-11
US20180320203A1 (en) 2018-11-08
US11572576B2 (en) 2023-02-07
US10570421B2 (en) 2020-02-25
WO2017077504A1 (en) 2017-05-11
US20210163998A1 (en) 2021-06-03
BR122023025807A2 (pt) 2024-01-16
CN108474010A (zh) 2018-08-31
US12258606B2 (en) 2025-03-25

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