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GB2458863A - Improved culture of stem cells - Google Patents

Improved culture of stem cells Download PDF

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Publication number
GB2458863A
GB2458863A GB0914193A GB0914193A GB2458863A GB 2458863 A GB2458863 A GB 2458863A GB 0914193 A GB0914193 A GB 0914193A GB 0914193 A GB0914193 A GB 0914193A GB 2458863 A GB2458863 A GB 2458863A
Authority
GB
United Kingdom
Prior art keywords
culture
cloning
stem cells
stem cell
conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0914193A
Other versions
GB0914193D0 (en
GB2458863B (en
Inventor
Velt Bergendahl
James A Thomson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39277243&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=GB2458863(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of GB0914193D0 publication Critical patent/GB0914193D0/en
Publication of GB2458863A publication Critical patent/GB2458863A/en
Application granted granted Critical
Publication of GB2458863B publication Critical patent/GB2458863B/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

While culture medium and systems have been described that permit the culture and proliferation of human embry-onic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.
GB0914193A 2007-01-17 2008-01-17 Improved culture of stem cells Active GB2458863B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88074707P 2007-01-17 2007-01-17
PCT/US2008/051347 WO2008089351A1 (en) 2007-01-17 2008-01-17 Improved culture of stem cells

Publications (3)

Publication Number Publication Date
GB0914193D0 GB0914193D0 (en) 2009-09-16
GB2458863A true GB2458863A (en) 2009-10-07
GB2458863B GB2458863B (en) 2011-10-12

Family

ID=39277243

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0914193A Active GB2458863B (en) 2007-01-17 2008-01-17 Improved culture of stem cells

Country Status (6)

Country Link
US (1) US7892830B2 (en)
JP (1) JP5419279B2 (en)
CA (1) CA2675445C (en)
GB (1) GB2458863B (en)
SE (1) SE0950586L (en)
WO (1) WO2008089351A1 (en)

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US8193235B2 (en) * 2009-06-12 2012-06-05 University Of Kansas Compositions and methods for establishing and maintaining stem cells in an undifferentiated state
US9005968B2 (en) * 2009-10-16 2015-04-14 The Scripps Research Institute Induction of pluripotent cells
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DK3633025T3 (en) 2009-11-12 2022-12-12 Technion Res & Dev Foundation CULTURE MEDIA, CELL CULTURES AND METHODS FOR CULTIVATING PLURIPOTENT STEM CELLS IN AN UNDIFFERENTIATED STATE
EP2507363A4 (en) * 2009-12-02 2013-10-30 Res Dev Foundation Selection of stem cell clones with defined differentiation capabilities
AU2011235212B2 (en) 2010-03-31 2014-07-31 The Scripps Research Institute Reprogramming cells
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KR101362639B1 (en) 2010-09-16 2014-02-13 연세대학교 산학협력단 Use of compounds for inducing differentiation of mesenchymal stem cells to chondrocytes
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
KR101975688B1 (en) 2010-12-22 2019-05-07 페이트 세러퓨틱스, 인코포레이티드 Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
WO2012112609A2 (en) * 2011-02-14 2012-08-23 Life Technologies Corporation Substrates and methods for staining live cells
EP2694644B1 (en) 2011-03-30 2018-01-24 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
EP2732029B1 (en) 2011-07-11 2019-01-16 FUJIFILM Cellular Dynamics, Inc. Methods for cell reprogramming and genome engineering
PL2773748T3 (en) 2011-11-04 2020-06-15 Memorial Sloan Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
US10238755B2 (en) 2011-11-30 2019-03-26 The Wistar Institute Of Anatomy And Biology Methods and compositions for regulation of cell aging, carcinogenesis and reprogramming
CN105358680B (en) 2013-04-03 2019-06-25 富士费勒姆细胞动力学有限公司 Methods and compositions for suspension culture of endodermal progenitor cells
JP6756610B2 (en) 2013-04-26 2020-09-16 メモリアル スローン ケタリング キャンサー センター Cortical interneurons and other neuronal cells generated by directing the differentiation of pluripotent cells and pluripotent cells
WO2015134652A1 (en) 2014-03-04 2015-09-11 Bahram Valamehr Improved reprogramming methods and cell culture platforms
EP3119881B1 (en) 2014-03-21 2023-03-01 FUJIFILM Cellular Dynamics, Inc. Production of midbrain dopaminergic neurons and methods for the use thereof
US10444227B2 (en) 2014-08-19 2019-10-15 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
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JP7263005B2 (en) 2015-10-16 2023-04-24 フェイト セラピューティクス，インコーポレイテッド A platform for the induction and maintenance of ground-state pluripotency
CN108367099B (en) 2015-10-21 2021-11-23 印第安纳大学研究与技术公司 Derivation of human skin organoids from pluripotent stem cells
CN108291197B (en) 2015-10-21 2022-09-16 印第安纳大学研究与技术公司 Method for generating sensory epithelium and sensory neurons of human inner ear
AU2016343682A1 (en) 2015-10-30 2018-06-14 The Regents Of The University Of California Methods of generating T-cells from stem cells and immunotherapeutic methods using the T-cells
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Also Published As

Publication number Publication date
GB0914193D0 (en) 2009-09-16
JP5419279B2 (en) 2014-02-19
JP2010516255A (en) 2010-05-20
SE0950586L (en) 2009-08-13
WO2008089351A1 (en) 2008-07-24
US20080171385A1 (en) 2008-07-17
US7892830B2 (en) 2011-02-22
GB2458863B (en) 2011-10-12
CA2675445C (en) 2014-11-04
CA2675445A1 (en) 2008-07-24

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