MX2018010283A - Metodo para producir factor de crecimiento de hepatocitos (hgf) activo. - Google Patents
Metodo para producir factor de crecimiento de hepatocitos (hgf) activo.Info
- Publication number
- MX2018010283A MX2018010283A MX2018010283A MX2018010283A MX2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A
- Authority
- MX
- Mexico
- Prior art keywords
- hgfa
- growth factor
- hepatocyte growth
- hgf
- pro
- Prior art date
Links
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 title abstract 4
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 108010043026 HGF activator Proteins 0.000 abstract 10
- 102100031465 Hepatocyte growth factor activator Human genes 0.000 abstract 10
- 238000000034 method Methods 0.000 abstract 3
- 239000012228 culture supernatant Substances 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000012679 serum free medium Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21034—Plasma kallikrein (3.4.21.34)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención proporciona un método para producir activador de factor de crecimiento de hepatocitos activo (HGFA) y factor de crecimiento de hepatocitos activo (HGF) sin usar suero animal. La presente invención se refiere a un método para producir HGFA activo sin usar suero animal. El método se caracteriza porque comprende una etapa de obtener un sobrenadante del cultivo que comprende pro-HGFA, cultivando células de mamífero que expresan activador de factor de crecimiento de hepatocitos inactivo (pro-HGFA) en un medio sin suero, y una etapa de ajustar a débilmente ácido el sobrenadante del cultivo que comprende pro-HGFA obtenido en la etapa anterior para convertir pro-HGFA en HGFA activo. La presente invención se refiere también a un método para producir HGF activo con HGFA producido por el método.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016054128 | 2016-03-17 | ||
| PCT/JP2017/010355 WO2017159722A1 (ja) | 2016-03-17 | 2017-03-15 | 活性型肝細胞増殖因子（ｈｇｆ）の製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2018010283A true MX2018010283A (es) | 2019-01-31 |
| MX395311B MX395311B (es) | 2025-03-25 |
Family
ID=59850449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018010283A MX395311B (es) | 2016-03-17 | 2017-03-15 | Metodo para producir factor de crecimiento de hepatocitos (hgf) activo. |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US11548926B2 (es) |
| EP (1) | EP3431590B1 (es) |
| JP (1) | JP6861201B2 (es) |
| KR (1) | KR102275262B1 (es) |
| CN (1) | CN109196099B (es) |
| AU (1) | AU2017232582B2 (es) |
| CA (1) | CA3014567C (es) |
| DK (1) | DK3431590T3 (es) |
| ES (1) | ES2894477T3 (es) |
| HU (1) | HUE055414T2 (es) |
| IL (1) | IL261066B (es) |
| LT (1) | LT3431590T (es) |
| MX (1) | MX395311B (es) |
| RU (1) | RU2747977C2 (es) |
| SG (1) | SG11201806843UA (es) |
| WO (1) | WO2017159722A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3431590B1 (en) | 2016-03-17 | 2021-07-28 | Eisai R&D Management Co., Ltd. | Method for producing activated hepatocyte growth factor (hgf) |
| CN110607304A (zh) * | 2019-07-25 | 2019-12-24 | 广州凌腾生物医药有限公司 | 肝细胞生长因子的重组表达方法 |
| CN110606883A (zh) * | 2019-07-25 | 2019-12-24 | 广州凌腾生物医药有限公司 | 肝细胞生长因子的制备方法 |
| WO2024096038A1 (ja) * | 2022-11-01 | 2024-05-10 | クリングルファーマ株式会社 | 活性型ｈｇｆの製造方法 |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2564486B2 (ja) | 1986-07-14 | 1996-12-18 | 修治 橋本 | 肝細胞増殖因子 |
| NZ232813A (en) | 1989-03-10 | 1992-08-26 | Snow Brand Milk Products Co Ltd | Human fibroblast glycoprotein, cell differentiation, blood vessel endothelial cell growth factor, cellular immunology inforcing factor of 78 or 74 thousand daltons plus or minus two thousand daltons |
| JP2706704B2 (ja) | 1989-06-05 | 1998-01-28 | 敏一 中村 | 組換ヒト肝実質細胞増殖因子 |
| JP3036782B2 (ja) | 1990-04-03 | 2000-04-24 | 三菱化学株式会社 | ヒト肝実質細胞増殖因子の生産方法および該因子を産生する形質転換体 |
| JP2577091B2 (ja) | 1989-08-11 | 1997-01-29 | 三菱化学株式会社 | 肝実質細胞増殖因子及びそれをコードする遺伝子 |
| JP2777678B2 (ja) | 1990-06-11 | 1998-07-23 | 敏一 中村 | 組換ヒト肝実質細胞増殖因子及びその製造方法 |
| JP3072628B2 (ja) | 1990-11-19 | 2000-07-31 | 敏一 中村 | ヒト肝実質細胞増殖因子をコードする染色体ｄｎａ |
| JP3213985B2 (ja) * | 1991-10-18 | 2001-10-02 | 三菱化学株式会社 | 新規なタンパク質 |
| JP3232714B2 (ja) * | 1992-11-20 | 2001-11-26 | 三菱化学株式会社 | 新規なタンパク質およびそれをコードする遺伝子 |
| CA2102463A1 (en) * | 1992-11-05 | 1994-05-06 | Mitsubishi Chemical Corporation | Protein and gene encoding said protein |
| DE69426292T2 (de) | 1993-02-23 | 2001-05-17 | Genentech, Inc. | Stabilisierung von mit organischen lösungsmittel behandelten polypeptiden mit einem hilfsstoff |
| JP2577091Y2 (ja) | 1993-06-23 | 1998-07-23 | 賢一 片山 | 分流装置の合成樹脂製サドル |
| JP3680114B2 (ja) * | 1993-09-17 | 2005-08-10 | 敏一 中村 | 脳神経障害治療剤 |
| JP2747979B2 (ja) | 1994-08-19 | 1998-05-06 | 雪印乳業株式会社 | ヒト由来の糖蛋白質からなる生理活性因子を有効成分とする医薬 |
| JP3927248B2 (ja) | 1995-07-11 | 2007-06-06 | 第一製薬株式会社 | Ｈｇｆ凍結乾燥製剤 |
| JP2859577B2 (ja) | 1996-03-27 | 1999-02-17 | 三菱化学株式会社 | 肝実質細胞増殖因子 |
| JP4463885B2 (ja) | 1996-12-05 | 2010-05-19 | 敏一 中村 | 劇症肝炎疾患治療剤 |
| JP2980888B2 (ja) | 1998-07-09 | 1999-11-22 | 三菱化学株式会社 | 肝実質細胞増殖因子をコードする遺伝子 |
| HK1044471A1 (en) | 1999-05-31 | 2002-10-25 | Mitsubishi Chemical Corporation | Freeze dried hgf preparations |
| JP3072628U (ja) | 2000-02-17 | 2000-10-24 | 輝夫 尾形 | ステンドグラス製文字入り記念楯 |
| JP4090236B2 (ja) * | 2000-12-05 | 2008-05-28 | 三菱化学株式会社 | 活性型肝細胞増殖因子アクティベーターに対する特異的抗体とその使用法 |
| US7045602B2 (en) * | 2000-12-05 | 2006-05-16 | Mitsubishi Chemical Corporation | Specific antibody directed to active hepatocyte growth factor activator and method for using the same |
| DE10204792A1 (de) | 2002-02-06 | 2003-08-14 | Merck Patent Gmbh | Lyophilisierte Zubereitung enthaltend Immuncytokine |
| NZ551908A (en) * | 2004-07-26 | 2009-10-30 | Genentech Inc | Methods and compositions for modulating hepatocyte growth factor HGF activation |
| WO2006063300A2 (en) * | 2004-12-10 | 2006-06-15 | Genentech, Inc. | Crystal structure of hepatocyte growth factor activator complexed with kunitz domain inhibitor |
| WO2007058776A2 (en) * | 2005-11-10 | 2007-05-24 | Receptor Biologix, Inc. | Hepatocyte growth factor intron fusion proteins |
| EP2014676B1 (en) | 2006-04-20 | 2012-03-07 | Kringle Pharma Inc. | Hgf precursor protein mutant and activated form thereof |
| CA2673260A1 (en) | 2006-12-20 | 2008-07-03 | Bayer Healthcare Llc | Factor vii and viia compositions |
| WO2008102452A1 (ja) | 2007-02-22 | 2008-08-28 | Kringle Pharma Inc. | Ｈｇｆ製剤 |
| EP2362767B1 (en) | 2008-10-29 | 2017-12-06 | Ablynx N.V. | Formulations of single domain antigen binding molecules |
| CN101649320B (zh) * | 2009-09-15 | 2011-06-15 | 上海交通大学医学院附属仁济医院 | 重组人源hgf激活因子及其应用 |
| US8759491B2 (en) * | 2009-10-19 | 2014-06-24 | Genentech, Inc. | Modulators of hepatocyte growth factor activator |
| WO2011102845A1 (en) | 2010-02-18 | 2011-08-25 | Transtech Pharma, Inc. | Rage fusion protein compositions and methods of use |
| JP2012051822A (ja) | 2010-08-31 | 2012-03-15 | Institute Of Physical & Chemical Research | 肺癌診断用ポリペプチド、肺癌の検出方法、および治療効果の評価方法 |
| US9272018B2 (en) | 2011-04-18 | 2016-03-01 | Kyoto University | Acute hepatic insufficiency depressant and method for evaluating drug efficacy thereof |
| JP6124347B2 (ja) | 2011-08-02 | 2017-05-10 | 石川 哲也 | ＴＧＦ−βシグナリング阻害剤および／またはＹ−２７６３２による肝前駆細胞の誘導方法および製造方法、並びに当該肝前駆細胞から肝細胞の製造方法 |
| AU2013201422B2 (en) * | 2012-01-23 | 2015-04-09 | Ablynx Nv | Sequences directed against hepatocyte growth factor (HFG) and polypeptides comprising the same for the treatment of cancers and/or tumors |
| CA2888472C (en) | 2012-10-20 | 2022-06-28 | Board Of Regents, The University Of Texas System | Cancer cell trap |
| RU2506946C1 (ru) | 2012-11-28 | 2014-02-20 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Воронежская государственная медицинская академия им. Н.Н. Бурденко" Министерства здравоохранения и социального развития Российской Федерации | Технология лиофилизации обогащенной тромбоцитами плазмы с сохранением жизнеспособности факторов tgf pdgf vegf |
| RU2693472C2 (ru) | 2014-04-28 | 2019-07-03 | Эйсай Ар Энд Ди Менеджмент Ко., Лтд. | Лиофилизированный состав на основе hgf |
| EP3431590B1 (en) | 2016-03-17 | 2021-07-28 | Eisai R&D Management Co., Ltd. | Method for producing activated hepatocyte growth factor (hgf) |
| EP3431992A4 (en) | 2016-03-18 | 2019-10-23 | Eisai R&D Management Co., Ltd. | PD MARKERS OF THE HEPATOCYTE GROWTH FACTOR (HGF) |
| CN208371461U (zh) | 2017-08-21 | 2019-01-15 | 潍坊鸿旺工贸有限公司 | 一种晾衣架用连接构件及晾衣架 |
-
2017
- 2017-03-15 EP EP17766718.5A patent/EP3431590B1/en active Active
- 2017-03-15 WO PCT/JP2017/010355 patent/WO2017159722A1/ja not_active Ceased
- 2017-03-15 RU RU2018130530A patent/RU2747977C2/ru active
- 2017-03-15 DK DK17766718.5T patent/DK3431590T3/da active
- 2017-03-15 HU HUE17766718A patent/HUE055414T2/hu unknown
- 2017-03-15 KR KR1020187024470A patent/KR102275262B1/ko active Active
- 2017-03-15 JP JP2018505972A patent/JP6861201B2/ja active Active
- 2017-03-15 ES ES17766718T patent/ES2894477T3/es active Active
- 2017-03-15 CA CA3014567A patent/CA3014567C/en active Active
- 2017-03-15 SG SG11201806843UA patent/SG11201806843UA/en unknown
- 2017-03-15 MX MX2018010283A patent/MX395311B/es unknown
- 2017-03-15 LT LTEPPCT/JP2017/010355T patent/LT3431590T/lt unknown
- 2017-03-15 AU AU2017232582A patent/AU2017232582B2/en active Active
- 2017-03-15 CN CN201780013057.7A patent/CN109196099B/zh active Active
- 2017-03-15 US US16/078,568 patent/US11548926B2/en active Active
-
2018
- 2018-08-08 IL IL261066A patent/IL261066B/en unknown
-
2022
- 2022-05-05 US US17/737,343 patent/US20220267393A1/en not_active Abandoned
-
2024
- 2024-02-01 US US18/429,738 patent/US12384824B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| MX395311B (es) | 2025-03-25 |
| KR20180127322A (ko) | 2018-11-28 |
| IL261066A (en) | 2018-10-31 |
| DK3431590T3 (da) | 2021-10-11 |
| IL261066B (en) | 2021-08-31 |
| EP3431590B1 (en) | 2021-07-28 |
| KR102275262B1 (ko) | 2021-07-12 |
| US11548926B2 (en) | 2023-01-10 |
| RU2018130530A (ru) | 2020-04-17 |
| RU2018130530A3 (es) | 2020-08-13 |
| CN109196099A (zh) | 2019-01-11 |
| WO2017159722A1 (ja) | 2017-09-21 |
| US20190062390A1 (en) | 2019-02-28 |
| CA3014567A1 (en) | 2017-09-21 |
| JP6861201B2 (ja) | 2021-04-21 |
| US20220267393A1 (en) | 2022-08-25 |
| HUE055414T2 (hu) | 2021-11-29 |
| LT3431590T (lt) | 2021-09-10 |
| EP3431590A1 (en) | 2019-01-23 |
| US20240218035A1 (en) | 2024-07-04 |
| AU2017232582A1 (en) | 2018-08-30 |
| JPWO2017159722A1 (ja) | 2019-01-24 |
| US12384824B2 (en) | 2025-08-12 |
| EP3431590A4 (en) | 2019-11-20 |
| CN109196099B (zh) | 2022-01-11 |
| ES2894477T3 (es) | 2022-02-14 |
| AU2017232582B2 (en) | 2022-07-14 |
| SG11201806843UA (en) | 2018-09-27 |
| RU2747977C2 (ru) | 2021-05-18 |
| CA3014567C (en) | 2024-02-20 |
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