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MX2018008351A - Metodos para preparar capsinoides por procesos biosinteticos. - Google Patents

Metodos para preparar capsinoides por procesos biosinteticos.

Info

Publication number
MX2018008351A
MX2018008351A MX2018008351A MX2018008351A MX2018008351A MX 2018008351 A MX2018008351 A MX 2018008351A MX 2018008351 A MX2018008351 A MX 2018008351A MX 2018008351 A MX2018008351 A MX 2018008351A MX 2018008351 A MX2018008351 A MX 2018008351A
Authority
MX
Mexico
Prior art keywords
capsinoids
making
methods
mixture
cellular system
Prior art date
Application number
MX2018008351A
Other languages
English (en)
Inventor
Chen Hui
Yu Xiaodan
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2018008351A publication Critical patent/MX2018008351A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/48—Separation; Purification; Stabilisation; Use of additives
    • C07C67/52—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation
    • C07C67/54—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation by distillation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/48—Separation; Purification; Stabilisation; Use of additives
    • C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/48—Separation; Purification; Stabilisation; Use of additives
    • C07C67/58—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/48—Separation; Purification; Stabilisation; Use of additives
    • C07C67/60—Separation; Purification; Stabilisation; Use of additives by treatment giving rise to chemical modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
    • C07C69/52—Esters of acyclic unsaturated carboxylic acids having the esterified carboxyl group bound to an acyclic carbon atom
    • C07C69/587—Monocarboxylic acid esters having at least two carbon-to-carbon double bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Se proporcionan en la presente métodos para preparar capsinoides incluyendo proporcionar una capsiato sintasa en una mezcla o sistema celular, alimentar 8-metil-6-nonenoil-CoA, ácido 6E-8-metilnonenoico o ácido 8- metilnonanoico a la mezcla o sistema celular, alimentar alcohol vainillílico a la mezcla o sistema celular, y recolectar capsinoides de la mezcla o sistema celular.
MX2018008351A 2016-01-07 2017-01-06 Metodos para preparar capsinoides por procesos biosinteticos. MX2018008351A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662276059P 2016-01-07 2016-01-07
PCT/US2017/012535 WO2017120473A1 (en) 2016-01-07 2017-01-06 Methods of making capsinoids by biosynthetic processes

Publications (1)

Publication Number Publication Date
MX2018008351A true MX2018008351A (es) 2018-12-06

Family

ID=59274312

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018008351A MX2018008351A (es) 2016-01-07 2017-01-06 Metodos para preparar capsinoides por procesos biosinteticos.

Country Status (10)

Country Link
US (1) US10655150B2 (es)
EP (1) EP3377057A4 (es)
JP (1) JP2019500877A (es)
KR (1) KR20180101445A (es)
CN (1) CN108472279A (es)
AU (1) AU2017205501A1 (es)
BR (1) BR112018013539A2 (es)
CA (1) CA3010716A1 (es)
MX (1) MX2018008351A (es)
WO (1) WO2017120473A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11459591B2 (en) 2016-07-19 2022-10-04 Conagen Inc. Method for the microbial production of specific natural capsaicinoids
CN111187161B (zh) * 2020-01-20 2021-10-01 中国科学技术大学 一种二氢辣椒素和二氢辣椒素酯的制备方法
IL305756A (en) * 2021-03-10 2023-11-01 Yeda Res & Dev An acyl-activating enzyme, and a cell, tissue, and organism that contain it are tensogens.
CN115960736B (zh) * 2023-03-01 2024-05-28 石河子大学 一种产香草胺和辣椒碱的酿酒酵母工程菌及其构建方法与应用
CN116837016B (zh) * 2023-08-29 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种构建生产辣椒素香草壬酰胺的重组大肠杆菌工程菌株的方法及重组菌株和应用

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958745A (en) 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
JP4043605B2 (ja) 1998-07-02 2008-02-06 丸善製薬株式会社 カプサイシン類縁体の製造法
US20070244192A1 (en) 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
DE10000978A1 (de) 2000-01-12 2001-07-26 Gvs Ges Fuer Erwerb Und Verwer Verfahren zur Erhöhung des Gehalts an Fettsäuren in Pflanzen und Mikroorganismen
US20040010817A1 (en) 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
JP3951008B2 (ja) 2002-01-17 2007-08-01 国立大学法人 岡山大学 カプサイシン分解合成酵素及びその生産方法
US20040033530A1 (en) 2002-04-08 2004-02-19 Awrey Donald E. High throughput purification, characterization and identification of recombinant proteins
GB0421937D0 (en) 2004-10-02 2004-11-03 Univ York Acyl CoA synthetases
KR101527739B1 (ko) * 2005-02-18 2015-06-12 아지노모토 가부시키가이샤 캡시노이드 조성물 및 이의 제조방법
WO2006100680A2 (en) 2005-03-22 2006-09-28 Hazera Genetics Ltd. Pepper plants having fruit with altered vitamin content
EP1917961A1 (en) * 2005-08-16 2008-05-07 Ajinomoto Co., Inc. Capsinoid containing composition
US20070220634A1 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
CA2644273A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
ES2307420B1 (es) * 2007-04-24 2009-10-20 Universidad De Cadiz Procedimiento de sintesis quimica de capsinoides.
BRPI0821508B1 (pt) 2007-12-11 2018-12-26 Synthetic Genomics Inc cultura celular compreendendo uma cianobactéria recombinante da espécie synechococcus elongatus e método para converter carbono inorgânico em ácidos graxos.
CN102065680B (zh) * 2008-06-23 2014-12-24 味之素株式会社 能生物合成辣椒素酯类物质的基因修饰植物
CN103842509B (zh) 2011-07-13 2016-06-08 加拿大国家研究委员会 用于烷酰基辅酶a合成的基因和蛋白质
US8951762B2 (en) 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
HK1199286A1 (en) 2011-08-08 2015-06-26 Evolva Sa Methods and materials for recombinant production of saffron compounds
CA2860416C (en) 2011-12-27 2024-04-30 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
RU2723620C2 (ru) 2012-07-13 2020-06-16 Калиста, Инк. Система, способы и композиции для биопереработки
CN103087998B (zh) 2012-10-30 2014-09-03 浙江工业大学 冬虫夏草合成十六烷基-辅酶a的酶、基因及其应用
US10184140B2 (en) 2013-01-23 2019-01-22 Iowa State University Research Foundation, Inc. Materials and methods for production of bi-functional fatty acids in recombinant bacteria
CN103173444A (zh) 2013-03-27 2013-06-26 广东省农业科学院蔬菜研究所 一种准确区分辣椒与甜椒的功能标记及其检测引物组和检测方法
MX2016005718A (es) 2013-11-01 2016-12-08 Conagen Inc Metodos de uso de acil-coa sintetasa para produccion biosintetica de acil-coas.
CN103725652B (zh) 2013-12-25 2016-06-29 无锡新和源发酵技术研究院有限公司 一种酰基辅酶a合成酶及其应用
KR20160125370A (ko) 2014-01-17 2016-10-31 코나겐 인크. 캡사이시노이드의 미생물적 생산을 위한 캡사이신 신타제의 사용 방법
WO2015160842A1 (en) * 2014-04-14 2015-10-22 Flex Pharma, Inc. Methods and formulatiions of capsaicinoids and capsinoids
EP3020819A1 (en) 2014-11-17 2016-05-18 Evonik Degussa GmbH Fatty acid and derivatives production
US11459591B2 (en) 2016-07-19 2022-10-04 Conagen Inc. Method for the microbial production of specific natural capsaicinoids
JP6986084B2 (ja) 2016-12-22 2021-12-22 コナジェン・インコーポレイテッドConagen Inc. ８−メチルノナン酸の微生物生成方法

Also Published As

Publication number Publication date
CA3010716A1 (en) 2017-07-13
CN108472279A (zh) 2018-08-31
EP3377057A4 (en) 2018-12-05
US20190010522A1 (en) 2019-01-10
AU2017205501A1 (en) 2018-07-05
JP2019500877A (ja) 2019-01-17
EP3377057A1 (en) 2018-09-26
US10655150B2 (en) 2020-05-19
KR20180101445A (ko) 2018-09-12
BR112018013539A2 (pt) 2018-12-04
WO2017120473A1 (en) 2017-07-13

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