[go: up one dir, main page]

MX2019002376A - Metodos de amplificacion digital del genoma completo. - Google Patents

Metodos de amplificacion digital del genoma completo.

Info

Publication number
MX2019002376A
MX2019002376A MX2019002376A MX2019002376A MX2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A
Authority
MX
Mexico
Prior art keywords
whole genome
methods
digital amplification
amplification
amplicons
Prior art date
Application number
MX2019002376A
Other languages
English (en)
Inventor
Xing Dong
Sunney Xie Xiaoliang
Han Chang Chi-
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of MX2019002376A publication Critical patent/MX2019002376A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente descripcion proporciona un metodo para la amplificacion del ADN genomico, tal como la amplificacion del genoma completo, incluyendo el uso de un sistema de transposasa para hacer fragmentos del ADN genomico, incluyendo los sitios de union del iniciador, aislar en aceite cada fragmento dentro de su propia microgota acuosa junto con los reactivos de amplificacion de PCR, amplificar cada fragmento dentro de su propia microgota acuosa, demulsificar las microgotas para obtener los amplicones y secuenciar los amplicones.
MX2019002376A 2016-08-31 2016-08-31 Metodos de amplificacion digital del genoma completo. MX2019002376A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/097520 WO2018039969A1 (en) 2016-08-31 2016-08-31 Methods of whole genome digital amplification

Publications (1)

Publication Number Publication Date
MX2019002376A true MX2019002376A (es) 2019-06-20

Family

ID=61299616

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019002376A MX2019002376A (es) 2016-08-31 2016-08-31 Metodos de amplificacion digital del genoma completo.

Country Status (9)

Country Link
US (1) US20210285038A1 (es)
EP (1) EP3507379A4 (es)
JP (1) JP6882453B2 (es)
CN (1) CN109923214A (es)
AU (1) AU2016421196A1 (es)
CA (1) CA3034959A1 (es)
MX (1) MX2019002376A (es)
RU (1) RU2736351C2 (es)
WO (1) WO2018039969A1 (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4397767A3 (en) 2012-08-14 2024-07-31 10X Genomics, Inc. Microcapsule compositions and methods
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124336A2 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Partitioning and processing of analytes and other species
JP6726659B2 (ja) 2014-04-10 2020-07-22 １０エックス ジェノミクス， インコーポレイテッド 試薬をカプセル化およびパーティション化するための流体デバイス、システム、および方法、ならびにそれらの応用
AU2015279617A1 (en) 2014-06-26 2017-01-12 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2016114970A1 (en) 2015-01-12 2016-07-21 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
EP4628582A3 (en) 2017-01-30 2025-12-24 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018217912A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
CN113614245B (zh) 2019-02-12 2024-08-27 10X基因组学有限公司 用于加工核酸分子的方法
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2021188889A1 (en) * 2020-03-20 2021-09-23 Mission Bio, Inc. Single cell workflow for whole genome amplification
WO2021222267A1 (en) * 2020-04-28 2021-11-04 President And Fellows Of Harvard College Systems and methods for determining viruses or other pathogens
RU2752840C1 (ru) * 2020-12-17 2021-08-09 Общество с ограниченной ответственностью «ДНК-дисплей» Способ штрихкодирования ампликонов при подготовке таргетных библиотек ДНК к массовому параллельному секвенированию
CN115807058B (zh) * 2022-12-02 2023-06-30 中国科学院水生生物研究所 一种低偏向的单精子全基因组扩增方法
CN118043478B (zh) * 2023-10-20 2025-03-14 北京昌平实验室 用于可靠的无创胚胎植入前基因检测的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2396245T3 (es) * 2003-01-29 2013-02-20 454 Life Sciences Corporation Método de amplificación y secuenciamiento de ácidos nucleicos
JP4773338B2 (ja) * 2003-03-07 2011-09-14 ルビコン ゲノミクス， インコーポレイテッド Ｄｎａ重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012166425A2 (en) * 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN107075509B (zh) * 2014-05-23 2021-03-09 数字基因公司 通过数字化转座子的单倍体组测定
US10017759B2 (en) * 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN105463066B (zh) * 2014-09-04 2019-05-17 中国科学院北京基因组研究所 一种dna扩增方法
CN108026575B (zh) * 2015-07-17 2022-08-19 哈佛学院董事及会员团体 扩增核酸序列的方法

Also Published As

Publication number Publication date
EP3507379A4 (en) 2020-05-13
WO2018039969A1 (en) 2018-03-08
CN109923214A (zh) 2019-06-21
JP6882453B2 (ja) 2021-06-02
RU2736351C2 (ru) 2020-11-16
AU2016421196A1 (en) 2019-03-21
CA3034959A1 (en) 2018-03-08
US20210285038A1 (en) 2021-09-16
JP2019531714A (ja) 2019-11-07
RU2019108270A3 (es) 2020-10-01
RU2019108270A (ru) 2020-10-01
EP3507379A1 (en) 2019-07-10

Similar Documents

Publication Publication Date Title
MX2019002376A (es) Metodos de amplificacion digital del genoma completo.
WO2015160895A3 (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
MX2019013993A (es) Amplificación de etiquetado extremo múltiple de ácidos nucleicos.
MX2018000729A (es) Métodos para amplificar las secuencias de ácido nucleico.
SG11202011467RA (en) Polynucleotides, reagents, and methods for nucleic acid hybridization
AU2017263886A1 (en) Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
IL249126B (en) A method for identifying and numbering nucleic acid sequence, expression, copies, or changes in DNA methylation, using a combination of nuclease, ligase, polymerase, and sequencing reactions
HK1253712A1 (zh) 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法
WO2008086381A3 (en) Isothermal dna amplification
MX2016016713A (es) Procesos y sistemas para el montaje de secuencias de acido nucleico.
MX376755B (es) Detección de ácidos nucleicos diana mediante el uso de hibridación.
HK1256342A1 (zh) 环境或生物样本中的核酸纯化
EP4527942A3 (en) Preserving genomic connectivity information in fragmented genomic dna samples
WO2014012107A3 (en) Human identifiation using a panel of snps
WO2019122879A3 (en) Methods for performing biological reactions
WO2016109799A3 (en) Method of rna isolation from clinical samples
WO2014143714A3 (en) Methods for one step nucleic acid amplification of non-eluted samples
GB2519465A (en) Solid matrix for one step nucleic acid amplification
HK1246690A1 (zh) 用於鉴定高度特异性核酸酶的方法和组合物
EP3283630A4 (en) Oligonucleotide fragment, and method as well as application of selective amplifiction of variant of target nucleic acid sequence using the same
PH12019501694A1 (en) Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
WO2018231955A3 (en) Isolation of target nucleic acids
SG11201809242VA (en) Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor
WO2015176006A3 (en) Genetic detection platform
EP3253888A4 (en) Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level