MX2017000772A - Metodo para extraer proteinas solubles de biomasa de microalgas. - Google Patents
Metodo para extraer proteinas solubles de biomasa de microalgas.Info
- Publication number
- MX2017000772A MX2017000772A MX2017000772A MX2017000772A MX2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A
- Authority
- MX
- Mexico
- Prior art keywords
- biomass
- soluble
- protein isolate
- soluble fraction
- kda
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 title abstract 6
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 abstract 1
- 238000000889 atomisation Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000008030 elimination Effects 0.000 abstract 1
- 238000003379 elimination reaction Methods 0.000 abstract 1
- 238000001704 evaporation Methods 0.000 abstract 1
- 230000008020 evaporation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000005189 flocculation Methods 0.000 abstract 1
- 230000016615 flocculation Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000001471 micro-filtration Methods 0.000 abstract 1
- 238000009928 pasteurization Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/009—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from unicellular algae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La invención se refiere a un método para preparar un aislado de proteínas a partir de la biomasa de microalgas del género Chlorella, caracterizado por que comprende las siguientes etapas: suministro de una biomasa de microalgas producida por fermentación; opcionalmente, lavado de la biomasa de manera que se eliminen los compuestos solubles intersticiales, permeabilización térmica de la biomasa a una temperatura de entre 50 y 150 °C, preferentemente de entre 100 y 150 °C, durante entre 10 segundos y 5 minutos, preferentemente entre 10 segundos y 1 minuto; eliminación de la biomasa permeabilizada de esta manera mediante una técnica de separación sólido-líquido seleccionada a partir del grupo constituido por filtración frontal o tangencial, floculación y centrifugación, más específicamente centrifugación multietapa, con el fin de obtener una fracción soluble; opcionalmente, recuperación y clarificación de la fracción soluble obtenida de este modo mediante microfiltración de tal manera que se eliminen los elementos insolubles residuales de ella; ultrafiltración de la fracción soluble con una membrana con un umbral de corte inferior a 5 kDa, preferentemente de entre 1 y 5 kDa, con el fin de producir un aislado de proteínas solubles; a continuación, evaporación, pasteurización y atomización de dicho aislado de proteínas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1456944 | 2014-07-18 | ||
| PCT/FR2015/051940 WO2016009145A1 (fr) | 2014-07-18 | 2015-07-16 | Procede d'extraction des proteines solubles de biomasses de microalgues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2017000772A true MX2017000772A (es) | 2017-04-27 |
Family
ID=53938354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017000772A MX2017000772A (es) | 2014-07-18 | 2015-07-16 | Metodo para extraer proteinas solubles de biomasa de microalgas. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170152294A1 (es) |
| EP (1) | EP3169695B1 (es) |
| JP (1) | JP2017521451A (es) |
| KR (1) | KR20170028353A (es) |
| CN (1) | CN106488927A (es) |
| BR (1) | BR112017001039A2 (es) |
| MX (1) | MX2017000772A (es) |
| WO (1) | WO2016009145A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX378520B (es) | 2014-07-18 | 2025-03-11 | Corbion Biotech Inc | Metodo para extraer proteinas solubles de biomasa de microalgas. |
| FR3031987B1 (fr) * | 2015-01-26 | 2019-05-24 | Corbion Biotech, Inc. | Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines |
| CN113924132B (zh) | 2019-05-07 | 2023-07-25 | 罗盖特公司 | 用于伤口护理的新型多糖基水凝胶支架 |
| CN111018069A (zh) * | 2019-12-09 | 2020-04-17 | 河南大禹水处理有限公司 | 一种回收微生物菌体蛋白的方法 |
| EP4507513A1 (fr) * | 2022-04-11 | 2025-02-19 | Algama | Procédé d'obtention d'un extrait protéique texturant à partir de microalgue |
| WO2025104748A1 (en) * | 2023-11-16 | 2025-05-22 | String Bio Private Limited | Method of preparing microbial protein concentrate |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1138073A1 (ru) * | 1976-12-02 | 1985-02-07 | Всесоюзный Научно-Исследовательский Биотехнический Институт | Способ разрушени клеточной оболочки хлореллы |
| JPH01180832A (ja) * | 1988-01-05 | 1989-07-18 | Sanwa Kagaku Kenkyusho Co Ltd | 高ヨウ素含有淡水クロレラからの熱水抽出物、その製法、およびその用途 |
| JP3143636B2 (ja) | 1991-09-11 | 2001-03-07 | 株式会社サン・クロレラ | 細胞破裂によるクロレラ細胞壁の破砕方法 |
| JP2000106826A (ja) * | 1998-10-01 | 2000-04-18 | Senmi Extract Kk | クロレラペプチド含有組成物 |
| CN1259335C (zh) * | 2004-03-27 | 2006-06-14 | 中国科学院水生生物研究所 | 一种水华蓝藻制备藻蓝蛋白的方法 |
| KR100657637B1 (ko) * | 2005-03-08 | 2006-12-19 | 진현진 | 클로렐라로부터 액상 추출물을 추출하는 방법 |
-
2015
- 2015-07-16 BR BR112017001039A patent/BR112017001039A2/pt not_active Application Discontinuation
- 2015-07-16 JP JP2017502826A patent/JP2017521451A/ja active Pending
- 2015-07-16 MX MX2017000772A patent/MX2017000772A/es unknown
- 2015-07-16 US US15/322,199 patent/US20170152294A1/en not_active Abandoned
- 2015-07-16 CN CN201580038031.9A patent/CN106488927A/zh active Pending
- 2015-07-16 WO PCT/FR2015/051940 patent/WO2016009145A1/fr not_active Ceased
- 2015-07-16 EP EP15753722.6A patent/EP3169695B1/fr active Active
- 2015-07-16 KR KR1020177000336A patent/KR20170028353A/ko not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP2017521451A (ja) | 2017-08-03 |
| BR112017001039A2 (pt) | 2017-11-14 |
| US20170152294A1 (en) | 2017-06-01 |
| EP3169695A1 (fr) | 2017-05-24 |
| CN106488927A (zh) | 2017-03-08 |
| WO2016009145A1 (fr) | 2016-01-21 |
| EP3169695B1 (fr) | 2019-11-13 |
| KR20170028353A (ko) | 2017-03-13 |
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