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MX2016002306A - Promotor u6 de polimerasa iii de soja y metodos de uso. - Google Patents

Promotor u6 de polimerasa iii de soja y metodos de uso.

Info

Publication number
MX2016002306A
MX2016002306A MX2016002306A MX2016002306A MX2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A
Authority
MX
Mexico
Prior art keywords
soybean
methods
polymerase iii
promoter
plants
Prior art date
Application number
MX2016002306A
Other languages
English (en)
Inventor
Zhongsen Li
Andrew Mark Cigan
Joshua K Young
Saverio Carl Falco
Zhan-Bin Liu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51493053&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016002306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Du Pont filed Critical Du Pont
Publication of MX2016002306A publication Critical patent/MX2016002306A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine

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  • Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
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  • Environmental Sciences (AREA)
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  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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  • Enzymes And Modification Thereof (AREA)
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  • Immunology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)

Abstract

La invención se refiere a secuencias reguladoras de la expresión génica de soja, específicamente al promotor de un gen de U6 polimerasa III de soja y fragmentos de este, y su uso para promover la expresión de uno o más fragmentos de ácidos nucleicos heterólogos en plantas. La invención describe, además, composiciones, constructos de polinucleótidos, células hospederas transformadas, plantas mutadas, plantas y semillas transgénicas que contienen el constructo recombinante con el promotor, y métodos para preparar y usarlas.
MX2016002306A 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso. MX2016002306A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361868706P 2013-08-22 2013-08-22
US201361882532P 2013-09-25 2013-09-25
US201461937045P 2014-02-07 2014-02-07
US201461953090P 2014-03-14 2014-03-14
US201462023239P 2014-07-11 2014-07-11
PCT/US2014/051782 WO2015026887A1 (en) 2013-08-22 2014-08-20 A soybean u6 polymerase iii promoter and methods of use

Publications (1)

Publication Number Publication Date
MX2016002306A true MX2016002306A (es) 2016-07-08

Family

ID=51493053

Family Applications (5)

Application Number Title Priority Date Filing Date
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
MX2016002306A MX2016002306A (es) 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso.
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2016002266A MX361154B (es) 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.

Family Applications After (3)

Application Number Title Priority Date Filing Date
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2016002266A MX361154B (es) 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Country Status (12)

Country Link
US (16) US10519457B2 (es)
EP (6) EP3036327B1 (es)
CN (5) CN120574876A (es)
AU (6) AU2014308896A1 (es)
BR (3) BR112016003561B8 (es)
CA (7) CA2922040C (es)
DK (1) DK3036327T3 (es)
ES (1) ES2729635T3 (es)
HU (1) HUE044916T2 (es)
LT (1) LT3036327T (es)
MX (5) MX2016002118A (es)
WO (4) WO2015026887A1 (es)

Families Citing this family (346)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX365030B (es) 2010-06-25 2019-05-21 Du Pont Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz.
BR112013024337A2 (pt) 2011-03-23 2017-09-26 Du Pont locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
CA2877290A1 (en) 2012-06-19 2013-12-27 Daniel F. Voytas Gene targeting in plants using dna viruses
WO2014065596A1 (en) 2012-10-23 2014-05-01 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
HRP20181648T1 (hr) 2013-04-16 2019-01-25 Regeneron Pharmaceuticals, Inc. Ciljana modifikacija genoma štakora
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
MX2016002118A (es) 2013-08-22 2016-06-28 Du Pont Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US10584358B2 (en) 2013-10-30 2020-03-10 North Carolina State University Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri
WO2015070083A1 (en) 2013-11-07 2015-05-14 Editas Medicine,Inc. CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS
MX388127B (es) 2013-12-11 2025-03-19 Regeneron Pharma Metodos y composiciones para la modificacion dirigida de un genoma.
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US10787654B2 (en) 2014-01-24 2020-09-29 North Carolina State University Methods and compositions for sequence guiding Cas9 targeting
US11186843B2 (en) * 2014-02-27 2021-11-30 Monsanto Technology Llc Compositions and methods for site directed genomic modification
US11439712B2 (en) 2014-04-08 2022-09-13 North Carolina State University Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes
WO2015189693A1 (en) * 2014-06-12 2015-12-17 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using crispr/cas9
BR112017000482A2 (pt) 2014-07-11 2017-11-07 Du Pont métodos para produzir uma planta mutante e para gerar uma planta, planta, semente, rna, métodos para produzir uma célula, para duplicar um fragmento gênico, para substituir uma primeira sequência promotora, para inserir um elemento regulador em uma sequência de nucleotídeos e para inserir um íntron em uma sequência de nucleotídeos, planta de milho e célula vegetal
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EP3186375A4 (en) 2014-08-28 2019-03-13 North Carolina State University Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
WO2016073433A1 (en) 2014-11-06 2016-05-12 E. I. Du Pont De Nemours And Company Peptide-mediated delivery of rna-guided endonuclease into cells
ES2731437T3 (es) * 2014-11-21 2019-11-15 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida mediante el uso de pares de ARN guías
KR102763527B1 (ko) 2014-12-03 2025-02-05 애질런트 테크놀로지스, 인크. 화학적 변형을 갖는 가이드 rna
JP6830437B2 (ja) 2014-12-10 2021-02-17 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 疾患を処置するための遺伝子改変された細胞、組織および臓器
US20170369902A1 (en) 2014-12-16 2017-12-28 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
US20180179523A1 (en) * 2014-12-18 2018-06-28 Integrated Dna Technologies, Inc. Crispr-based compositions and methods of use
JP2017538427A (ja) 2014-12-18 2017-12-28 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド Ｃｒｉｓｐｒ系組成物及び使用方法
EP4219730A1 (en) 2015-01-27 2023-08-02 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for conducting site-specific modification on entire plant via gene transient expression
PL3250691T3 (pl) * 2015-01-28 2023-11-27 Caribou Biosciences, Inc. Hybrydowe polinukleotydy dna/rna crispr i przydatne sposoby
WO2016126417A1 (en) 2015-02-04 2016-08-11 Pioneer Hi Bred International Inc NOVEL Bt TOXIN RECEPTORS AND METHODS OF USE
SG11201706766WA (en) 2015-02-23 2017-09-28 Crispr Therapeutics Ag Materials and methods for treatment of hemoglobinopathies
WO2016135559A2 (en) 2015-02-23 2016-09-01 Crispr Therapeutics Ag Materials and methods for treatment of human genetic diseases including hemoglobinopathies
WO2016137774A1 (en) * 2015-02-25 2016-09-01 Pioneer Hi-Bred International Inc Composition and methods for regulated expression of a guide rna/cas endonuclease complex
KR102194612B1 (ko) 2015-03-16 2020-12-23 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 비-유전성 물질을 이용한 식물 게놈의 부위-특이적인 변형 방법
CN107429258A (zh) * 2015-03-19 2017-12-01 先锋国际良种公司 用于加速的性状渐渗的方法和组合物
US10450576B2 (en) 2015-03-27 2019-10-22 E I Du Pont De Nemours And Company Soybean U6 small nuclear RNA gene promoters and their use in constitutive expression of small RNA genes in plants
ES2884838T3 (es) * 2015-04-06 2021-12-13 Univ Leland Stanford Junior ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
WO2016186953A1 (en) 2015-05-15 2016-11-24 Pioneer Hi Bred International Inc Guide rna/cas endonuclease systems
DE102015006335A1 (de) 2015-05-19 2016-11-24 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
EP3095870A1 (en) * 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
DE102015014252A1 (de) 2015-11-05 2017-05-11 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
KR20220139447A (ko) 2015-05-29 2022-10-14 노쓰 캐롤라이나 스테이트 유니버시티 크리스퍼 핵산을 사용하여 박테리아, 고세균, 조류 및 효모를 스크리닝하는 방법
WO2016196887A1 (en) 2015-06-03 2016-12-08 Board Of Regents Of The University Of Nebraska Dna editing using single-stranded dna
US11155823B2 (en) 2015-06-15 2021-10-26 North Carolina State University Methods and compositions for efficient delivery of nucleic acids and RNA-based antimicrobials
US20180305707A1 (en) * 2015-06-26 2018-10-25 Pioneer Hi-Bred International, Inc. Gos2 regulatory elements and use thereof
AU2016285724A1 (en) * 2015-06-29 2017-11-16 Ionis Pharmaceuticals, Inc. Modified CRISPR RNA and modified single CRISPR RNA and uses thereof
WO2017004279A2 (en) * 2015-06-29 2017-01-05 Massachusetts Institute Of Technology Compositions comprising nucleic acids and methods of using the same
CN108472314A (zh) 2015-07-31 2018-08-31 明尼苏达大学董事会 修饰的细胞和治疗方法
US9580727B1 (en) 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
JP2018527920A (ja) 2015-08-14 2018-09-27 インスティテュート・オブ・ジェネティクス・アンド・ディヴェロプメンタル・バイオロジー、チャイニーズ・アカデミー・オブ・サイエンシズＩｎｓｔｉｔｕｔｅ ｏｆ Ｇｅｎｅｔｉｃｓ ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔａｌ Ｂｉｏｌｏｇｙ， Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ 部位特異的ヌクレオチド置換によりグリホサート耐性イネを取得するための方法
CA2992488A1 (en) 2015-08-28 2017-03-09 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
WO2017053729A1 (en) 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
US11286480B2 (en) 2015-09-28 2022-03-29 North Carolina State University Methods and compositions for sequence specific antimicrobials
WO2017059045A1 (en) * 2015-09-30 2017-04-06 Pioneer Hi-Bred International, Inc. Plant epsp synthases and methods of use
US11732269B2 (en) * 2015-10-02 2023-08-22 Monsanto Technology Llc Recombinant maize B chromosome sequence and uses thereof
CA3000917A1 (en) 2015-10-09 2017-04-13 Monsanto Technology Llc Rna-guided nucleases and uses thereof
JP7011590B2 (ja) * 2015-10-12 2022-02-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 細胞内での遺伝子組換えおよび相同組換えの増加のための保護ｄｎａ鋳型および使用方法
WO2018071362A1 (en) 2016-10-13 2018-04-19 Pioneer Hi-Bred International, Inc. Generating northern leaf blight resistant maize
CN108137658A (zh) 2015-10-16 2018-06-08 先锋国际良种公司 产生对北方叶枯病具有增强的抗性的玉蜀黍植物
JP2018531024A (ja) 2015-10-20 2018-10-25 パイオニア ハイ−ブレッド インターナショナル， イン マーカーフリーゲノム改変のための方法および組成物
US9677090B2 (en) 2015-10-23 2017-06-13 Caribou Biosciences, Inc. Engineered nucleic-acid targeting nucleic acids
EP3159407A1 (en) * 2015-10-23 2017-04-26 Silence Therapeutics (London) Ltd Guide rnas, methods and uses
US10167457B2 (en) 2015-10-23 2019-01-01 President And Fellows Of Harvard College Nucleobase editors and uses thereof
AU2016349504B2 (en) * 2015-11-04 2023-02-09 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
EP3371306B8 (en) 2015-11-04 2023-02-22 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
CN106811479B (zh) * 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用
BR112018011174A2 (pt) 2015-11-30 2018-11-21 Du Pont polinucleotídeo recombinante, construto de dna, célula heteróloga, planta transgênica ou célula de planta, semente da planta transgênica, método para expressar um polinucleotídeo em uma planta e método para aprimorar a transcrição de um polinucleotídeo em uma célula hospedeira
BR112018011242B1 (pt) * 2015-12-02 2023-11-28 Ceres, Inc. Método de produção de uma planta com um traço agronômico desejável
CN109310784B (zh) * 2015-12-07 2022-08-19 阿克生物公司 用于制备和使用指导核酸的方法和组合物
JP6888906B2 (ja) * 2015-12-11 2021-06-18 株式会社豊田中央研究所 生物体のゲノムの改変方法及びその利用
EP3387134B1 (en) 2015-12-11 2020-10-14 Danisco US Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
US11761007B2 (en) * 2015-12-18 2023-09-19 The Scripps Research Institute Production of unnatural nucleotides using a CRISPR/Cas9 system
EP3390631B1 (en) 2015-12-18 2020-04-08 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
EP4257694A3 (en) 2015-12-22 2023-12-06 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
WO2017112620A1 (en) 2015-12-22 2017-06-29 North Carolina State University Methods and compositions for delivery of crispr based antimicrobials
BR112018013679A2 (pt) 2016-01-11 2019-01-22 Univ Leland Stanford Junior proteínas quiméricas e métodos de regulação de expressão gênica
MX2018008345A (es) 2016-01-11 2018-12-06 Univ Leland Stanford Junior Proteínas quiméricas y métodos de inmunoterapia.
US20190017067A1 (en) * 2016-01-12 2019-01-17 Regents Of The University Of Minnesota Glyphosate tolerant plants having modified 5-enolpyruvylshikimate-3-phosphate synthase gene regulation
WO2017132239A1 (en) 2016-01-26 2017-08-03 Pioneer Hi-Bred International, Inc. Waxy corn
CA3014036A1 (en) * 2016-02-09 2017-08-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
EP3426778A1 (en) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
CN107312821A (zh) * 2016-04-25 2017-11-03 中国检验检疫科学研究院 用于转基因大米m12品系特异性基因成分精准定量检测的引物探针、试剂盒及方法
MX388294B (es) 2016-05-27 2025-03-19 Aadigen Llc Peptidos y nanoparticulas para suministro intracelular de moleculas editoras de genoma.
WO2017207589A1 (en) 2016-06-01 2017-12-07 Kws Saat Se Hybrid nucleic acid sequences for genome engineering
US10767175B2 (en) * 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN109312317A (zh) 2016-06-14 2019-02-05 先锋国际良种公司 Cpf1内切核酸酶用于植物基因组修饰的用途
CA3018430A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
WO2018005589A1 (en) * 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN106350536B (zh) * 2016-08-28 2021-02-12 浙江大学 一种植物杂交体系及其应用
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
CN109936977A (zh) * 2016-09-14 2019-06-25 孟山都技术公司 用于通过单倍体诱导来进行基因组编辑的方法和组合物
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018068053A2 (en) 2016-10-07 2018-04-12 Integrated Dna Technologies, Inc. S. pyogenes cas9 mutant genes and polypeptides encoded by same
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
EP4338799A3 (en) 2016-10-18 2024-06-05 Regents of the University of Minnesota Tumor infiltrating lymphocytes and methods of therapy
WO2018081728A1 (en) * 2016-10-31 2018-05-03 Emendobio Inc. Compositions for genome editing
CA3042259A1 (en) 2016-11-04 2018-05-11 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
CN108070611B (zh) * 2016-11-14 2021-06-29 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
JP2019535287A (ja) 2016-11-22 2019-12-12 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｃｒｉｓｐｒ／ｃｐｆ１システム及び方法
US11236348B2 (en) 2016-11-29 2022-02-01 Pioneer Hi-Bred International, Inc. Transcriptional terminators for gene expression in plants
US9816093B1 (en) 2016-12-06 2017-11-14 Caribou Biosciences, Inc. Engineered nucleic acid-targeting nucleic acids
WO2018103686A1 (zh) * 2016-12-07 2018-06-14 中国科学院上海生命科学研究院 叶绿体基因组编辑方法
WO2018106470A1 (en) * 2016-12-08 2018-06-14 Syngenta Participations Ag Methods for improving transformation frequency
EP3551757A1 (en) * 2016-12-08 2019-10-16 Intellia Therapeutics, Inc. Modified guide rnas
US11293022B2 (en) 2016-12-12 2022-04-05 Integrated Dna Technologies, Inc. Genome editing enhancement
AU2017379402B2 (en) * 2016-12-22 2022-03-10 Toolgen Incorporated Oleic acid-enriched plant body having genetically modified FAD2 and production method therefor
EP3559232A1 (en) 2016-12-22 2019-10-30 Intellia Therapeutics, Inc. Compositions and methods for treating alpha-1 antitrypsin deficiency
CA3047163A1 (en) * 2016-12-22 2018-06-28 Monsanto Technology Llc Genome editing-based crop engineering and production of brachytic plants
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
CN106834341B (zh) * 2016-12-30 2020-06-16 中国农业大学 一种基因定点突变载体及其构建方法和应用
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
EP3568484A1 (en) * 2017-01-11 2019-11-20 Yeda Research and Development Co. Ltd Targeted recombination between homologous chromosomes and uses thereof
WO2018140899A1 (en) 2017-01-28 2018-08-02 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11307139B2 (en) 2017-03-03 2022-04-19 Pioneer Hi-Bred International, Inc. Non-destructive assay for soybean seeds using near infrared analysis
CN107066944B (zh) * 2017-03-06 2019-11-05 北京数码视讯科技股份有限公司 一种手指指根的定位方法及装置
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
BR112019019655A2 (pt) 2017-03-23 2020-04-22 Harvard College editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico
BR112019020311A2 (pt) * 2017-03-30 2020-04-28 Pioneer Hi Bred Int epsp sintases de planta melhoradas e métodos de uso
WO2018183607A1 (en) 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
WO2018183878A1 (en) 2017-03-31 2018-10-04 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
EP3615552A1 (en) 2017-04-24 2020-03-04 DuPont Nutrition Biosciences ApS Methods and compositions of anti-crispr proteins for use in plants
US20200407737A1 (en) 2017-05-03 2020-12-31 KWS SAAT SE & Co. KGaA Use of crispr-cas endonucleases for plant genome engineering
AU2018263195B2 (en) * 2017-05-05 2022-04-07 Suzhou Qi Biodesign Biotechnology Company Limited Methods for isolating cells without the use of transgenic marker sequences
BR112019023377A2 (pt) * 2017-05-11 2020-06-16 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Criação de um gene resistente a herbicida e uso do mesmo
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
EP3624581A4 (en) * 2017-05-17 2021-03-10 Cold Spring Harbor Laboratory Compositions and methods for generating weak alleles in plants
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
EP3630973A1 (en) * 2017-05-31 2020-04-08 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
WO2018224861A1 (en) 2017-06-07 2018-12-13 International Rice Research Institute Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile gramineae plants and related materials and methods
WO2018237197A2 (en) * 2017-06-21 2018-12-27 Noble Research Institute, Llc Crispr-mediated selective gene disruption in plants
EP3642342A4 (en) 2017-06-23 2021-03-17 Cornell University RNA MOLECULES, METHODS FOR PRODUCING CIRCULAR RNA AND TREATMENT METHODS
EP3645021A4 (en) 2017-06-30 2021-04-21 Intima Bioscience, Inc. ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
RU2020111575A (ru) * 2017-08-22 2021-09-23 Напиджен, Инк. Модификация генома органелл с использованием направляемой полинуклеотидом эндонуклеазы
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US20200199609A1 (en) * 2017-09-14 2020-06-25 Pioneer Hi-Bred International, Inc. Compositions and methods for stature modification in plants
US12416015B2 (en) 2017-09-15 2025-09-16 The Board Of Trustees Of The Leland Stanford Junior University Multiplex production and barcoding of genetically engineered cells
WO2019053725A1 (en) * 2017-09-18 2019-03-21 Futuragene Israel Ltd. TISSUE-SPECIFIC EXPRESSION CONTROL OF DELLA POLYPEPTIDES
US11555199B2 (en) 2017-09-19 2023-01-17 Tropic Biosciences UK Limited Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
JP2020536515A (ja) 2017-09-25 2020-12-17 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド 組織優先的プロモーター及びその使用方法
US11603536B2 (en) 2017-09-29 2023-03-14 Inari Agriculture Technology, Inc. Methods for efficient maize genome editing
BR112020007343A2 (pt) 2017-10-13 2020-10-06 Pioneer Hi-Bred International, Inc. método para gerar um embrião de planta haploide, micrósporo embriogênico, embrioide ou tecido embriogênico, célula vegetal compreendendo um cassete de expressão, população de células vegetais
AU2018352592C1 (en) 2017-10-16 2025-09-25 Beam Therapeutics, Inc. Uses of adenosine base editors
WO2019084306A1 (en) * 2017-10-26 2019-05-02 University Of Florida Research Foundation, Inc. METHOD FOR REDUCING GENOMIC COMPLEXITY AND DETECTING POLYMORPHISMS
CA3074617A1 (en) 2017-11-16 2019-05-23 Dow Agrosciences Llc Plant promoter for transgene expression
US12161674B2 (en) 2017-12-05 2024-12-10 Vertex Pharmaceuticals Incorporated CRISPR-CAS9 modified CD34+ human hematopoietic stem and progenitor cells and uses thereof
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
KR102705104B1 (ko) 2017-12-15 2024-09-09 다니스코 유에스 인크. Cas9 변이체 및 사용 방법
CA3085914A1 (en) * 2017-12-22 2019-06-27 G+Flas Life Sciences Chimeric genome engineering molecules and methods
EP3728330A4 (en) 2017-12-23 2021-12-08 Uwell Biopharma Inc. PHARMACEUTICAL COMPOSITION OF CHEMICAL RECEPTOR AND ASSOCIATED PROCESS
WO2019133371A1 (en) 2017-12-27 2019-07-04 Pioneer Hi-Bred International, Inc. Transformation of dicot plants
JP7082142B2 (ja) * 2017-12-28 2022-06-07 日立造船株式会社 全固体電池、その製造方法および加工装置
CN109971763A (zh) * 2017-12-28 2019-07-05 未名生物农业集团有限公司 花期调控基因cmp1和相关的载体及其应用
GB201800184D0 (en) * 2018-01-05 2018-02-21 Univ Bristol Compositions and methods for delivery of nucleic acid plant cells
US11634722B2 (en) 2018-01-22 2023-04-25 Inari Agriculture Technology, Inc. Plant gene editing systems, methods, and compositions
US20240318171A1 (en) * 2018-01-26 2024-09-26 Integrated Dna Technologies, Inc. Crispr-based compositions and methods of use
US11220694B1 (en) 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11802288B1 (en) 2018-01-29 2023-10-31 Inari Agriculture Technology, Inc. Methods for efficient soybean genome editing
US11566236B2 (en) 2018-02-05 2023-01-31 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
MA51788A (fr) 2018-02-05 2020-12-16 Vertex Pharma Substances et méthodes pour traiter des hémoglobinopathies
EP3752647B1 (en) * 2018-02-15 2022-05-25 The Broad Institute, Inc. Cell data recorders and uses thereof
US20210105962A1 (en) * 2018-02-22 2021-04-15 Elsoms Developments Ltd Methods and compositions relating to maintainer lines
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
US11390876B2 (en) 2018-03-09 2022-07-19 Pioneer Hi-Bred International, Inc. Compositions and methods for modification of fatty acids in soybean
WO2019177976A1 (en) 2018-03-12 2019-09-19 Pioneer Hi-Bred International, Inc. Methods for plant transformation
AU2019239957A1 (en) * 2018-03-19 2020-09-10 Bayer Healthcare Llc Novel RNA-programmable endonuclease systems and uses thereof
US12209289B2 (en) 2018-03-23 2025-01-28 Pioneer Hi-Bred International, Inc. Methods of identifying, selecting, and producing disease resistant crops
CN110396523B (zh) * 2018-04-23 2023-06-09 中国科学院分子植物科学卓越创新中心 一种重复片段介导的植物定点重组方法
WO2019205919A1 (zh) * 2018-04-28 2019-10-31 青岛清原化合物有限公司 一种生物突变体库的构建方法
CN112088018A (zh) * 2018-05-07 2020-12-15 先锋国际良种公司 用于在植物细胞基因组中同源定向修复双链断裂的方法和组合物
WO2019213910A1 (en) * 2018-05-10 2019-11-14 Syngenta Participations Ag Methods and compositions for targeted editing of polynucleotides
WO2019222545A1 (en) 2018-05-16 2019-11-21 Synthego Corporation Methods and systems for guide rna design and use
CN108642077A (zh) * 2018-05-18 2018-10-12 江苏省农业科学院 基于CRISPR/Cas9基因编辑技术选育绿豆不育突变体的方法及专用gRNA
CA3097902A1 (en) * 2018-05-21 2019-11-28 Pioneer Hi-Bred International, Inc. Modification of glutamine synthetase to improve yield in plants
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
BR112020023853A2 (pt) 2018-05-25 2021-04-13 Pioneer Hi-Bred International, Inc. Sistemas e métodos para melhoramento aprimorado por modulação das taxas de recombinação
WO2019231924A1 (en) * 2018-05-29 2019-12-05 Monsanto Technology Llc Transgenic plants with enhanced traits
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
WO2020006112A1 (en) 2018-06-26 2020-01-02 Regents Of The University Of Minnesota Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations
EP3833747A1 (en) 2018-06-28 2021-06-16 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
WO2020006423A1 (en) 2018-06-29 2020-01-02 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
AU2019292919A1 (en) * 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
BR112021002792A2 (pt) 2018-08-13 2021-06-29 Pioneer Hi-Bred International, Inc. novos receptores de toxinas inseticidas e métodos de uso
AU2019363487A1 (en) * 2018-08-30 2021-04-15 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
US11459551B1 (en) 2018-08-31 2022-10-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US12534743B2 (en) 2018-08-31 2026-01-27 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
CN109136258A (zh) * 2018-09-06 2019-01-04 先正达参股股份有限公司 小麦中基因编辑效率的优化
CA3110093A1 (en) 2018-09-14 2020-03-19 Pioneer Hi-Bred International, Inc. Fertility restoration in plants
EP3853361A1 (en) * 2018-09-20 2021-07-28 Sanofi Intron-based universal cloning methods and compositions
US10711267B2 (en) 2018-10-01 2020-07-14 North Carolina State University Recombinant type I CRISPR-Cas system
WO2020072254A1 (en) 2018-10-01 2020-04-09 North Carolina State University Recombinant type i crispr-cas system and uses thereof for killing target cells
WO2020072253A1 (en) 2018-10-01 2020-04-09 North Carolina State University Recombinant type i crispr-cas system and uses thereof for screening for variant cells
US12264313B2 (en) 2018-10-01 2025-04-01 North Carolina State University Recombinant type I CRISPR-Cas system and uses thereof for genome modification and alteration of expression
US20220033833A1 (en) * 2018-10-02 2022-02-03 Monsanto Technology Llc Compositions and methods for transferring biomolecules to wounded cells
EP3866583A1 (en) * 2018-10-16 2021-08-25 Pioneer Hi-Bred International, Inc. Genome edited fine mapping and causal gene identification
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
AU2019369418A1 (en) 2018-10-31 2021-03-18 Pioneer Hi-Bred International, Inc. Compositions and methods for Ochrobactrum-mediated gene editing
US20210395758A1 (en) 2018-10-31 2021-12-23 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
EP3874048A1 (en) * 2018-11-01 2021-09-08 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020097445A1 (en) 2018-11-09 2020-05-14 Inari Agriculture, Inc. Rna-guided nucleases and dna binding proteins
US11518999B1 (en) 2018-11-09 2022-12-06 Inari Agriculture Technology, Inc. Plant transformation
WO2020117553A1 (en) * 2018-12-04 2020-06-11 Syngenta Crop Protection Ag Gene silencing via genome editing
EP3896155A4 (en) * 2018-12-12 2022-08-17 Kyushu University, National University Corporation Production method for genome-edited cells
WO2020123887A2 (en) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US11453885B1 (en) 2019-02-19 2022-09-27 Inari Agriculture Technology, Inc. Plant transformation
AU2020239058B2 (en) 2019-03-11 2024-02-01 Colorado State University Research Foundation Herbicide tolerant plants and production and detection of same
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
CN114127285B (zh) 2019-03-19 2024-09-10 布罗德研究所股份有限公司 编辑核苷酸序列的方法和组合物
JP7743308B2 (ja) 2019-03-27 2025-09-24 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド 植物外稙片の形質転換
US12031149B2 (en) * 2019-03-27 2024-07-09 Emendobio Inc. Compositions and methods for promoting gene editing of CXCR4 gene
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
MX2021012158A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
AU2020263573A1 (en) * 2019-04-24 2021-10-28 Oregon Health & Science University Promoter sequence and related products and uses thereof
US11692197B2 (en) 2019-05-06 2023-07-04 Inari Agriculture Technology, Inc. Delivery of biological molecules to plant cells
CN110241121B (zh) * 2019-05-21 2022-03-29 南京农业大学 大豆E3泛素连接酶GmNLA1编码基因的应用
CA3141568A1 (en) * 2019-06-19 2020-12-24 BetterSeeds Ltd. A method for increasing cannabis yield via gene editing
MX2021014861A (es) 2019-06-25 2022-06-22 Inari Agriculture Tech Inc Edicion genomica de reparacion dependiente de homologia mejorada.
WO2021003410A1 (en) * 2019-07-03 2021-01-07 Napigen, Inc. Organelle genome modification
US20210047651A1 (en) * 2019-07-12 2021-02-18 The Regents Of The University Of California Compositions and methods for targeted insertion of selectable marker-free dna sequence in plants
US11746354B2 (en) 2019-07-19 2023-09-05 Inari Agriculture Technology, Inc. Homology dependent repair genome editing
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
US20250381301A1 (en) * 2019-08-19 2025-12-18 Minghong Zhong Conjugates of Guide RNA-Cas Protein Complex
EP3798301A1 (en) * 2019-09-25 2021-03-31 KWS SAAT SE & Co. KGaA Promoter repression
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021081367A1 (en) 2019-10-23 2021-04-29 Pairwise Plants Services, Inc. Compositions and methods for rna-templated editing in plants
MX2022005328A (es) 2019-11-05 2022-07-21 Pairwise Plants Services Inc Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.
CN121109454A (zh) * 2019-11-06 2025-12-12 青岛清原化合物有限公司 在生物体内创制新基因的方法及应用
US12385054B2 (en) 2019-11-19 2025-08-12 Protalix Ltd. Removal of constructs from transformed cells
US12031140B2 (en) * 2019-11-25 2024-07-09 Syngenta Crop Protection Ag Glycine regulatory elements and uses thereof
CA3159799A1 (en) * 2019-12-02 2021-06-10 Prashant MALI Engineering circular guide rnas
US12410439B2 (en) 2019-12-17 2025-09-09 Pioneer Hi-Bred International, Inc. Reduced stature maize and mads-box transcription factors
CN111057713A (zh) * 2019-12-18 2020-04-24 广东省微生物研究所（广东省微生物分析检测中心） 一种适用于埃德菌FS110的CRISPR/Cas9载体及其构建方法和应用
EP4087600A4 (en) * 2020-01-09 2024-01-24 Pioneer Hi-Bred International, Inc. TWO-STEP GENE EXCHANGE
US12157892B2 (en) * 2020-01-22 2024-12-03 Pioneer Hi-Bred International, Inc. Dominant CRISPR inverted-repeat alleles to down-regulate gene expression in heterozygous plants
WO2021162970A1 (en) * 2020-02-12 2021-08-19 Pioneer Hi-Bred International, Inc. Cas-mediated homology directed repair in somatic plant tissue
BR112022016917A2 (pt) 2020-02-24 2022-10-25 Pioneer Hi Bred Int Recombinação homóloga intragenômica
AU2021226415A1 (en) 2020-02-28 2022-09-15 Pioneer Hi-Bred International, Inc. Sorghum doubled haploid production system
WO2021183874A1 (en) * 2020-03-13 2021-09-16 Pairwise Plants Services, Inc. Methods of introducing variation into plants and products produced therefrom
BR112022019960A2 (pt) * 2020-03-30 2022-12-13 Inari Agriculture Tech Inc Método de modificação de um gene endógeno, método de modificação da expressão de um gene endógeno, célula vegetal, planta de soja, parte da planta, tecido ou tecido do calo, método para obter a célula vegetal, polinucleotídeo isolado, ácido nucleico recombinante
WO2021202061A2 (en) 2020-04-02 2021-10-07 Pioneer Hi-Bred International, Inc. Novel insecticidal toxin receptors and methods of use
CN116096901B (zh) 2020-04-15 2025-12-02 先锋国际良种公司 植物病原体效应子和疾病抗性基因鉴定、组合物和使用方法
TW202208626A (zh) * 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
EP4149243A1 (en) * 2020-05-13 2023-03-22 Nunhems B.V. Method for obtaining mutant plants by targeted mutagenesis
EP4162057A4 (en) * 2020-06-03 2024-06-12 Pioneer Hi-Bred International, Inc. Maize event dp-915635-4 and methods for detection thereof
CN111778274A (zh) * 2020-06-09 2020-10-16 新疆生产建设兵团第六师农业科学研究所 一种通过基因编辑提高番茄耐贮藏性的方法
CA3175936A1 (en) 2020-06-12 2021-12-16 Pioneer Hi-Bred International, Inc. Alteration of seed composition in plants
EP4168561A4 (en) 2020-07-20 2024-08-21 Flagship Pioneering, Inc. VIROID-DERIVED POLYNUCLEOTIDES FOR PLANT MODIFICATIONS
US11369073B2 (en) 2020-07-31 2022-06-28 Inari Agriculture Technology, Inc. INIR12 transgenic maize
US12529062B2 (en) 2020-07-31 2026-01-20 Inari Agriculture Technology, Inc. INIR12 transgenic maize
US11214811B1 (en) 2020-07-31 2022-01-04 Inari Agriculture Technology, Inc. INIR6 transgenic maize
US11326177B2 (en) 2020-07-31 2022-05-10 Inari Agriculture Technology, Inc. INIR12 transgenic maize
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean
US20240011042A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites
US11359210B2 (en) 2020-07-31 2022-06-14 Inari Agriculture Technology, Inc. INIR12 transgenic maize
WO2022031720A1 (en) * 2020-08-03 2022-02-10 Arcadia Biosciences, Inc. Dna constructs containing rna polymerase iii promoters from cannabis, and methods of their use
CN116134143A (zh) 2020-08-18 2023-05-16 先锋国际良种公司 多种抗病基因及其基因组堆叠件
WO2022038536A1 (en) 2020-08-18 2022-02-24 International Rice Research Institute Methods of increasing outcrossing rates in gramineae
EP4210475A4 (en) 2020-09-10 2025-03-05 Monsanto Technology LLC ENHANCING GENE EDITING AND SITE-SPECIFIC INTEGRATION EVENTS USING MEIOTIC AND GERMLINE PROMOTERS
KR102579767B1 (ko) * 2020-09-21 2023-09-18 서울대학교산학협력단 eIF4E1 유전자 교정에 의해 포티바이러스 저항성이 증가된 유전체 교정 토마토 식물체의 제조방법 및 상기 방법에 의해 제조된 포티바이러스 저항성이 증가된 유전체 교정 토마토 식물체
US11976291B2 (en) 2020-09-28 2024-05-07 Inari Agriculture Technology, Inc. Genetically enhanced maize plants
US11198885B1 (en) 2020-09-28 2021-12-14 Inari Agriculture Technology, Inc. Genetic regulatory element
US12188031B2 (en) 2020-09-28 2025-01-07 Inari Agriculture Technology, Inc. Genetic regulatory element
US20230374447A1 (en) 2020-10-05 2023-11-23 Protalix Ltd. Dicer-like knock-out plant cells
CA3197672A1 (en) * 2020-10-14 2022-04-21 Pioneer Hi-Bred International, Inc. Engineered cas endonuclease variants for improved genome editing
CN112481259B (zh) * 2020-11-24 2022-09-16 南昌大学 两种甘薯U6基因启动子IbU6的克隆与应用
CN116867362A (zh) * 2020-12-11 2023-10-10 国际人造丝公司 由纤维素酯组合物形成的泡沫
WO2022233271A1 (en) * 2021-05-02 2022-11-10 Qingdao Kingagroot Chemical Compound Co., Ltd. Method for generating new gene in organism and use thereof
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
JP2024524924A (ja) 2021-07-02 2024-07-09 トロピック バイオサイエンシーズ ユーケー リミテッド バナナ果実における褐変の遅延又は防止
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
UY39997A (es) 2021-11-01 2023-05-15 Flagship Pioneering Innovations Vii Llc Polinucleótidos para modificar organismos
EP4441208A1 (en) 2021-11-30 2024-10-09 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
KR20240135651A (ko) 2022-01-20 2024-09-11 플래그쉽 파이어니어링 이노베이션스 Vii, 엘엘씨 유기체를 변형하기 위한 폴리뉴클레오티드
EP4490299A1 (en) * 2022-03-07 2025-01-15 Inedita Bio, Inc. Methods and compositions for intron mediated- expression of regulatory elements for trait development
CN114836446B (zh) * 2022-04-26 2023-12-19 上海交通大学 一种抗草甘膦的植物及其制法
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4548351A1 (en) 2022-06-30 2025-05-07 Pioneer Hi-Bred International, Inc. Artificial intelligence-mediated methods and systems for genome editing
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020360A1 (en) 2022-07-18 2024-01-25 Pairwise Plants Services, Inc. Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc'
CA3261642A1 (en) * 2022-07-27 2024-02-01 Pioneer Hi-Bred International, Inc RNA-Trapped Genome Editing Guide
WO2024023578A1 (en) 2022-07-28 2024-02-01 Institut Pasteur Hsc70-4 in host-induced and spray-induced gene silencing
EP4569122A2 (en) 2022-08-09 2025-06-18 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing
EP4627076A1 (en) 2022-12-01 2025-10-08 Danisco Us Inc Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system
EP4627078A1 (en) 2022-12-01 2025-10-08 Danisco US Inc. Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
EP4627077A1 (en) 2022-12-01 2025-10-08 Danisco US Inc. Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
WO2024123786A1 (en) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants
EP4638471A1 (en) 2022-12-21 2025-10-29 Donald Danforth Plant Science Center Antimicrobial peptides and modifications thereof
CN115762641B (zh) * 2023-01-10 2023-04-07 天津极智基因科技有限公司 一种指纹图谱构建方法及系统
EP4684008A1 (en) 2023-03-20 2026-01-28 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition
CN121443140A (zh) 2023-04-21 2026-01-30 非营利性组织佛兰芒综合大学生物技术研究所 用于作物产量提升的等位基因组合
AR132585A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Sistemas de amplificación de arn satélite endornavirales para plantas
AR132591A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de secoviridae artificiales
AR132592A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de ghabrivirales artificiales
AR132586A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de amalgavirus artificiales
AR132588A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de tymovirales artificiales
WO2024229395A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Partitiviral satellite rna amplification systems for plants
AR132589A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de solemoviridae artificiales
WO2024229347A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Artificial tombusviridae satellite rnas
WO2024229362A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc ARTIFICIAL MARTELLIVIRALES SATELLITE RNAs
AU2024270764A1 (en) 2023-05-15 2025-12-04 Nchroma Bio, Inc. Compositions and methods for epigenetic regulation of hbv gene expression
WO2025021702A1 (en) 2023-07-21 2025-01-30 Alia Therapeutics S.R.L. Cas9 orthologue nuclease and uses thereof
WO2025038723A1 (en) 2023-08-14 2025-02-20 The Texas A&M University System Corn endotype-derived polypeptides capable of metabolizing fumonisin
WO2025059184A1 (en) 2023-09-12 2025-03-20 Pioneer Hi-Bred International, Inc. Methods and compositions for generating genome-edited paternal doubled haploids
WO2025076141A1 (en) 2023-10-03 2025-04-10 Inari Agriculture Technology, Inc. Viral delivery of grna to the scion
WO2025132372A1 (en) 2023-12-21 2025-06-26 Technische Universität München Plant sensor twa1 as molecular thermogenetic control switch and for improvement of thermotolerance and modifying of senescence
WO2025168705A1 (en) 2024-02-08 2025-08-14 Vib Vzw Means and methods for the production of saponins with endosomal escape-enhancing properties
WO2025184759A1 (en) 2024-03-04 2025-09-12 Pioneer Hi-Bred International, Inc. Maize plants comprising resistance to gray leaf spot and compositions and methods for selecting and producing the same
US20250382332A1 (en) 2024-06-12 2025-12-18 Invaio Sciences, Inc. Novel alpha-factor based peptides with antifungal activity
WO2025259861A2 (en) 2024-06-12 2025-12-18 Invaio Sciences, Inc. Antimicrobial peptides against citrus greening
WO2025259976A1 (en) 2024-06-14 2025-12-18 Pioneer Hi-Bred International, Inc. Deamination polyproteins and methods of use thereof
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides
CN119842730B (zh) * 2024-12-20 2025-10-24 中国科学院遗传与发育生物学研究所 一种调控大豆根瘤共生固氮的基因GmPMT及其应用

Family Cites Families (245)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1230061A (en) 1916-02-25 1917-06-12 Joseph A Blanchette Oil-can.
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5196525A (en) 1987-01-13 1993-03-23 University Of British Columbia DNA construct for enhancing the efficiency of transcription
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5202422A (en) 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
ATE212667T1 (de) 1990-04-26 2002-02-15 Aventis Cropscience Nv Neuer bacillusthuringsiensis stamm und sein für insektentoxin kodierendes gen
US5478369A (en) 1990-06-12 1995-12-26 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
DE69227911T2 (de) 1991-08-02 1999-05-12 Kubota Corp., Tokio/Tokyo Neuer mikroorganismus und insektizid
US5604121A (en) 1991-08-27 1997-02-18 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
ZA927576B (en) 1991-10-04 1993-04-16 Univ North Carolina State Pathogen-resistant transgenic plants.
TW261517B (es) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
EP0616644B1 (en) 1991-12-04 2003-07-02 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5474896A (en) 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
AU5407594A (en) 1992-11-17 1994-06-08 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
DE69428290T2 (de) 1993-01-13 2002-04-18 Pioneer Hi-Bred International, Inc. Derivate von alpha-hordothionin mit höherem behalt an lysin
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5837850A (en) 1994-04-21 1998-11-17 Pioneer Hi-Bred International, Inc. Regulatory element conferring tapetum specificity
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US7262055B2 (en) * 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
CA2160529A1 (en) 1994-10-14 1996-04-15 Toshihiko Iizuka Bacillus strain and harmful organism controlling agents
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
JPH11506329A (ja) 1995-06-02 1999-06-08 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド α−ホルドチオニンの高メチオニン誘導体
CA2222615A1 (en) 1995-06-02 1996-12-05 Gururaj A. Rao High threonine derivatives of .alpha.-hordothionin
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
AU3495297A (en) 1996-07-08 1998-02-02 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
AU728086B2 (en) 1996-11-01 2001-01-04 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US7053282B1 (en) 1998-02-09 2006-05-30 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
ATE342985T1 (de) 1998-02-26 2006-11-15 Pioneer Hi Bred Int Mais alpha-tubulin 3-18 promoter
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
AU5489099A (en) 1998-08-20 2000-03-14 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6504083B1 (en) 1998-10-06 2003-01-07 Pioneer Hi-Bred International, Inc. Maize Gos-2 promoters
HUP0300187A3 (en) 1999-04-29 2004-11-29 Syngenta Ltd Haslemere Herbicide resistant plants
US20100293669A2 (en) * 1999-05-06 2010-11-18 Jingdong Liu Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement
US7868149B2 (en) * 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) * 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
US20070083945A1 (en) 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
US6627797B1 (en) * 2000-03-21 2003-09-30 The Texas A&M University System Maize lipoxygenase polynucleotide and methods of use
US6683231B2 (en) 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
US7612251B2 (en) 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
WO2002026789A2 (en) 2000-09-26 2002-04-04 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7517975B2 (en) 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
AU8786201A (en) 2000-09-29 2002-04-08 Syngenta Ltd Herbicide resistant plants
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20090100536A1 (en) * 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
US20030186281A1 (en) 2001-12-21 2003-10-02 Wolfgang Hillen Modified tetracycline repressor protein compositions and methods of use
EP2374879A3 (en) 2002-04-22 2012-01-11 E. I. du Pont de Nemours and Company Promoter and plasmid system for genetic engineering
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
US20060206949A1 (en) 2003-01-28 2006-09-14 Sylvain Arnould Custom-made meganuclease and use thereof
US20040231016A1 (en) 2003-02-19 2004-11-18 Commonwealth Scientific And Industrial Research Organization Efficient gene silencing in plants using short dsRNA sequences
US7230161B2 (en) 2003-06-23 2007-06-12 Pioneer Hi-Bred International, Inc. Engineering single-gene-controlled staygreen potential into plants utilizing ACC synthase from maize
IES20030696A2 (en) 2003-09-23 2005-03-23 Carsphairn Ltd A pipeline apparatus
DE602004018824D1 (de) 2003-11-18 2009-02-12 Bayer Bioscience Nv Verbesserte gezielte dna-insertion in pflanzen
EP1699931B1 (en) 2003-12-22 2009-09-16 Pioneer Hi-Bred International, Inc. Maize metallothionein 2 promoter and methods of use
CA2554644C (en) 2004-02-02 2015-06-16 Pioneer Hi-Bred International, Inc. Ap2 domain transcription factor odp2 (ovule development protein 2) and methods of use
US7292055B2 (en) 2005-04-21 2007-11-06 Endicott Interconnect Technologies, Inc. Interposer for use with test apparatus
BRPI0612862A2 (pt) 2005-07-18 2010-11-30 Pioneer Hi Bred Int polinucleotìdeo isolado, método para obtenção de uma célula transformada, método para obtenção de uma planta transformada, método de produção de recombinação de dna de sìtio especìfico, biblioteca isolada, método de geração de biblioteca de moléculas e método para selecionar sìtio de recombinação frt modificado
US20070016985A1 (en) 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Particle Preparation for Direct-Delivery Transformation
DK2341149T3 (en) 2005-08-26 2017-02-27 Dupont Nutrition Biosci Aps Use of CRISPR-associated genes (Cas)
AU2006302969B2 (en) 2005-10-13 2011-09-22 Monsanto Technology, Llc Methods for producing hybrid seed
ES2539616T3 (es) 2005-10-18 2015-07-02 Precision Biosciences Meganucleasa diseñada racionalmente con afinidad de formación de dímeros alterada
NZ597682A (en) † 2006-01-12 2014-02-28 Incima Ipco B V Epsps mutants
AR060217A1 (es) 2006-03-31 2008-06-04 Pioneer Hi Bred Int Genes de maiz para controlar el crecimiento y tamano de organos en plantas y su uso en la mejora de plantas de cultivo
KR101447300B1 (ko) 2006-08-11 2014-10-07 몬산토 테크놀로지 엘엘씨 안트라닐레이트 신타제의 엽록체를 표적으로 하는 발현에 의한 고-트립토판 옥수수의 생산
EP3067425A1 (en) 2006-10-31 2016-09-14 E. I. du Pont de Nemours and Company Soybean event dp-305423-1 and constructs for the generation thereof
NZ578090A (en) 2006-12-29 2012-03-30 Dow Agrosciences Llc In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls, and transformation thereof
AU2008258254B2 (en) 2007-06-07 2014-07-03 Agriculture And Agri-Food Canada Nanocarrier based plant transfection and transduction
EP2568048A1 (en) 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US7919676B2 (en) 2007-08-03 2011-04-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
EP2190282A2 (en) 2007-08-29 2010-06-02 Monsanto Technology, LLC Methods for incorporating multiple genes in a crop plant
SI2205749T1 (sl) * 2007-09-27 2016-09-30 Dow Agrosciences, Llc Konstruirani proteini s cinkovim prstom, usmerjeni v 5-enolpiruvil-šikimat-3-fosfat-sintazne gene
JP5507459B2 (ja) 2007-10-05 2014-05-28 ダウ アグロサイエンシィズ エルエルシー 植物細胞に分子性物質を移行させる方法
US7667023B2 (en) 2007-10-30 2010-02-23 MS Technologies Promoter and vectors for plant transformation and methods of using same
US8697857B2 (en) 2007-11-20 2014-04-15 E. I. Du Pont De Nemours And Company Soybean EF1A2 promoter and its use in constitutive expression of transgenic genes in plants
US8546553B2 (en) 2008-07-25 2013-10-01 University Of Georgia Research Foundation, Inc. Prokaryotic RNAi-like system and methods of use
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
MX2011003766A (es) 2008-10-28 2011-04-27 Pioneer Hi Bred Int Proteinas represoras responsivas a sulfonilurea.
CA2747124C (en) 2008-12-17 2018-09-04 Dow Agrosciences Llc Targeted integration into the zp15 locus
HRP20150564T1 (hr) 2009-04-07 2015-08-28 Dow Agrosciences Llc Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza
US8466341B2 (en) 2009-05-04 2013-06-18 Pioneer Hi-Bred International, Inc. Maize 17KD oleosin seed-preferred regulatory element
WO2011082310A2 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
AU2010339404B2 (en) 2009-12-30 2016-01-28 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
US20110203012A1 (en) 2010-01-21 2011-08-18 Dotson Stanton B Methods and compositions for use of directed recombination in plant breeding
JP5922094B2 (ja) 2010-03-31 2016-05-24 ダウ アグロサイエンシィズ エルエルシー 生体分子の植物細胞中への送達のための植物ペプチドγ−ゼイン
BR112012028805A2 (pt) 2010-05-10 2019-09-24 The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc composições de endorribonuclease e métodos de uso das mesmas.
EP2580334A1 (en) 2010-06-09 2013-04-17 E.I. Du Pont De Nemours And Company Regulatory sequences for modulating transgene expression in plants
WO2012006439A2 (en) 2010-07-07 2012-01-12 Dow Agrosciences Llc Production of functionalized linear dna cassette and quantum dot/nanoparticle mediated delivery in plants
CA3065095C (en) 2010-07-07 2023-02-14 Dow Agrosciences Llc Method for introgressing a trait into a plant
US8478663B2 (en) 2010-07-28 2013-07-02 True Fit Corporation Fit recommendation via collaborative inference
CN103476934B (zh) 2011-02-15 2016-05-18 先锋国际良种公司 根优选的启动子以及使用方法
JP6067671B2 (ja) 2011-03-23 2017-01-25 ダウ アグロサイエンシィズ エルエルシー 植物におけるイメージングおよび送達適用に適した量子ドット担体ペプチドコンジュゲート体
BR112013024337A2 (pt) 2011-03-23 2017-09-26 Du Pont locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
US9726249B2 (en) 2011-05-13 2017-08-08 Mississippi State University Shock mitigating materials and methods utilizing spiral shaped elements
US20140113376A1 (en) 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes
BR112013033176A2 (pt) 2011-06-21 2018-06-12 E I Du Pont De Nemouras And Company método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão
EP2732040A2 (en) 2011-07-15 2014-05-21 E. I. Du Pont de Nemours and Company Novel plant terminator sequences
CN103842994A (zh) 2011-08-01 2014-06-04 标记公司 异步分布式数据库管理的系统和方法
US10538775B2 (en) 2011-08-02 2020-01-21 Pioneer Hi-Bred International, Inc. Terminator sequence for gene expression in plants
CA2844101A1 (en) 2011-08-03 2013-02-07 E. I. Du Pont De Nemours And Company Methods and compositions for targeted integration in a plant
CN103981149A (zh) 2011-08-22 2014-08-13 拜尔作物科学公司 修饰植物基因组的方法和手段
CA2853775A1 (en) 2011-10-31 2013-05-10 Pioneer Hi-Bred International, Inc. Improving plant drought tolerance, nitrogen use efficiency and yield
US20130210151A1 (en) 2011-11-07 2013-08-15 University Of Western Ontario Endonuclease for genome editing
WO2013090734A1 (en) 2011-12-15 2013-06-20 Dow Agrosciences Llc Method for improved transformation using agrobacterium
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
CN104203973A (zh) 2012-03-13 2014-12-10 先锋国际良种公司 植物中雄性育性的遗传减少
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
BR112014026203A2 (pt) 2012-04-23 2017-07-18 Bayer Cropscience Nv engenharia do genoma direcionado nas plantas
MX369788B (es) 2012-05-02 2019-11-21 Dow Agrosciences Llc Modificacion dirigida de deshidrogenasa de malato.
US20150184173A1 (en) * 2012-05-04 2015-07-02 E I Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
CN104487577A (zh) 2012-05-18 2015-04-01 先锋国际良种公司 用于调控表达的诱导型启动子序列及使用方法
PT3241902T (pt) † 2012-05-25 2018-05-28 Univ California Métodos e composições para modificação de adn alvo dirigida por arn e para modulação dirigida por arn de transcrição
US9725729B2 (en) 2012-06-04 2017-08-08 Pioneer Hi-Bred International, Inc. SB-actin terminator sequence for gene expression in plants
CA2877290A1 (en) * 2012-06-19 2013-12-27 Daniel F. Voytas Gene targeting in plants using dna viruses
CA2877892A1 (en) 2012-06-29 2014-01-03 Pioneer Hi-Bred International, Inc. Manipulation of serine/threonine protein phosphatases for crop improvement
KR102530118B1 (ko) 2012-07-25 2023-05-08 더 브로드 인스티튜트, 인코퍼레이티드 유도 ｄｎａ 결합 단백질 및 게놈 교란 도구 및 이의 적용
US20140196170A1 (en) 2012-08-30 2014-07-10 Salk Institute For Biological Studies Ethylene gas signaling in plants
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20140096284A1 (en) 2012-10-01 2014-04-03 Iowa State University Research Foundation, Inc. Method for the delivery of molecules lyophilized onto microparticles to plant tissues
WO2014065596A1 (en) 2012-10-23 2014-05-01 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
CA2890160A1 (en) * 2012-10-31 2014-05-08 Cellectis Coupling herbicide resistance with targeted insertion of transgenes in plants
CN108715602A (zh) 2012-12-06 2018-10-30 西格马-奥尔德里奇有限责任公司 基于crispr的基因组修饰和调控
WO2014093479A1 (en) 2012-12-11 2014-06-19 Montana State University Crispr (clustered regularly interspaced short palindromic repeats) rna-guided control of gene regulation
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
ES2553782T3 (es) 2012-12-12 2015-12-11 The Broad Institute, Inc. Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias
EP4234696A3 (en) 2012-12-12 2023-09-06 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
KR20150105634A (ko) 2012-12-12 2015-09-17 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 개선된 시스템, 방법 및 효소 조성물의 유전자 조작 및 최적화
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
DK2784162T3 (en) 2012-12-12 2015-07-13 Broad Inst Inc Design of systems, methods and optimized control manipulations for sequence manipulation
SG10201707569YA (en) 2012-12-12 2017-10-30 Broad Inst Inc Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications
AR093980A1 (es) 2012-12-13 2015-07-01 Dow Agrosciences Llc Genes de precision que tienen como objetivo un locus particular en el maiz
DK3553174T3 (da) 2012-12-17 2025-08-04 Harvard College Rna-guided modificering af humant genom
JP6491113B2 (ja) 2013-02-25 2019-03-27 サンガモ セラピューティクス， インコーポレイテッド ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物
WO2014164466A1 (en) 2013-03-12 2014-10-09 E. I. Du Pont De Nemours And Company Methods for the identification of variant recognition sites for rare-cutting engineered double-strand-break-inducing agents and compositions and uses thereof
NZ712727A (en) 2013-03-14 2017-05-26 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
HK1214306A1 (zh) 2013-03-15 2016-07-22 Regents Of The University Of Minnesota 采用crispr/cas系统的植物基因组的工程改造
EP3744842A1 (en) 2013-03-15 2020-12-02 The General Hospital Corporation Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing
AU2014233519B2 (en) 2013-03-15 2020-05-14 Cibus Europe B.V. Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
CN115261411A (zh) 2013-04-04 2022-11-01 哈佛学院校长同事会 利用CRISPR/Cas系统的基因组编辑的治疗性用途
WO2014186686A2 (en) 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
US20150067922A1 (en) 2013-05-30 2015-03-05 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
AU2014287393B2 (en) 2013-07-09 2020-10-22 President And Fellows Of Harvard College Multiplex RNA-guided genome engineering
CN110819658B (zh) 2013-07-10 2024-03-22 哈佛大学校长及研究员协会 用于RNA向导的基因调节和编辑的正交Cas9蛋白
PT3019619T (pt) 2013-07-11 2021-11-11 Modernatx Inc Composições que compreendem polinucleótidos sintéticos que codificam proteínas relacionadas com crispr e sgarn sintéticos e métodos de utilização
WO2015021426A1 (en) 2013-08-09 2015-02-12 Sage Labs, Inc. A crispr/cas system-based novel fusion protein and its application in genome editing
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US12054725B2 (en) 2013-08-22 2024-08-06 E.I. Du Pont De Nemours And Company U6 polymerase III promoter and methods of use
MX2016002118A (es) 2013-08-22 2016-06-28 Du Pont Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
WO2015070083A1 (en) 2013-11-07 2015-05-14 Editas Medicine,Inc. CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS
US20160298096A1 (en) 2013-11-18 2016-10-13 Crispr Therapeutics Ag Crispr-cas system materials and methods
CN103667338B (zh) 2013-11-28 2016-01-27 中国科学院遗传与发育生物学研究所 一种玉米基因组定点改造方法
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2015109752A1 (en) 2014-01-21 2015-07-30 The Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Modified plants
CA2936646C (en) 2014-01-24 2024-04-30 North Carolina State University Methods and compositions for sequences guiding cas9 targeting
CN109971826B (zh) 2014-01-31 2022-12-30 斯威夫特生物科学股份有限公司 用于加工dna底物的改进方法
US11186843B2 (en) 2014-02-27 2021-11-30 Monsanto Technology Llc Compositions and methods for site directed genomic modification
EP3152323B1 (en) 2014-06-05 2024-10-23 Qiagen GmbH Optimization of dna amplification reactions
WO2015189693A1 (en) 2014-06-12 2015-12-17 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using crispr/cas9
WO2015200378A1 (en) 2014-06-23 2015-12-30 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
BR112017000482A2 (pt) 2014-07-11 2017-11-07 Du Pont métodos para produzir uma planta mutante e para gerar uma planta, planta, semente, rna, métodos para produzir uma célula, para duplicar um fragmento gênico, para substituir uma primeira sequência promotora, para inserir um elemento regulador em uma sequência de nucleotídeos e para inserir um íntron em uma sequência de nucleotídeos, planta de milho e célula vegetal
CA2954686A1 (en) 2014-07-11 2016-01-14 Pioneer Hi-Bred International, Inc. Agronomic trait modification using guide rna/cas endonuclease systems and methods of use
EP3186375A4 (en) 2014-08-28 2019-03-13 North Carolina State University Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
PL3250691T3 (pl) 2015-01-28 2023-11-27 Caribou Biosciences, Inc. Hybrydowe polinukleotydy dna/rna crispr i przydatne sposoby
WO2016137774A1 (en) 2015-02-25 2016-09-01 Pioneer Hi-Bred International Inc Composition and methods for regulated expression of a guide rna/cas endonuclease complex
CN107429258A (zh) 2015-03-19 2017-12-01 先锋国际良种公司 用于加速的性状渐渗的方法和组合物
US10450576B2 (en) 2015-03-27 2019-10-22 E I Du Pont De Nemours And Company Soybean U6 small nuclear RNA gene promoters and their use in constitutive expression of small RNA genes in plants
WO2016186953A1 (en) 2015-05-15 2016-11-24 Pioneer Hi Bred International Inc Guide rna/cas endonuclease systems
DE102015006335A1 (de) 2015-05-19 2016-11-24 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
WO2016196738A1 (en) 2015-06-02 2016-12-08 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
WO2017015015A1 (en) 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
AR105776A1 (es) 2015-08-21 2017-11-08 Monsanto Technology Llc Recombinación mejorada de loci genómicos
CA3000917A1 (en) 2015-10-09 2017-04-13 Monsanto Technology Llc Rna-guided nucleases and uses thereof
EP4089175A1 (en) 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
JP2018531024A (ja) 2015-10-20 2018-10-25 パイオニア ハイ−ブレッド インターナショナル， イン マーカーフリーゲノム改変のための方法および組成物
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
EP3397757A4 (en) 2015-12-29 2019-08-28 Monsanto Technology LLC Novel CRISPR-ASSOCIATED TRANSPOSASES AND USES THEREOF
WO2017132239A1 (en) 2016-01-26 2017-08-03 Pioneer Hi-Bred International, Inc. Waxy corn
EP3426778A1 (en) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
GB201610041D0 (en) 2016-06-08 2016-07-20 Oxford Genetics Ltd Methods
CN109312317A (zh) 2016-06-14 2019-02-05 先锋国际良种公司 Cpf1内切核酸酶用于植物基因组修饰的用途
CA3018430A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
EP3601576A1 (en) 2017-03-24 2020-02-05 CureVac AG Nucleic acids encoding crispr-associated proteins and uses thereof
EP3615665B1 (en) 2017-04-24 2025-11-26 International N&H Denmark ApS Novel anti-crispr genes and proteins and methods of use
EP3615552A1 (en) 2017-04-24 2020-03-04 DuPont Nutrition Biosciences ApS Methods and compositions of anti-crispr proteins for use in plants
CA3078845A1 (en) 2017-10-09 2019-04-18 Pioneer Hi-Bred International, Inc. Type i-e crispr-cas systems for eukaryotic genome editing
EP3701013A4 (en) 2017-10-25 2021-08-04 Monsanto Technology LLC TARGETED ENDONUCLEASE ACTIVITY OF RNA-GUIDED ENDONUCLEASE CASX IN EUKARYONTS
US11970719B2 (en) 2017-11-01 2024-04-30 The Regents Of The University Of California Class 2 CRISPR/Cas compositions and methods of use
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019168953A1 (en) 2018-02-27 2019-09-06 President And Fellows Of Harvard College Evolved cas9 variants and uses thereof
WO2019177976A1 (en) 2018-03-12 2019-09-19 Pioneer Hi-Bred International, Inc. Methods for plant transformation
CN112088018A (zh) 2018-05-07 2020-12-15 先锋国际良种公司 用于在植物细胞基因组中同源定向修复双链断裂的方法和组合物
WO2019217816A1 (en) 2018-05-11 2019-11-14 Pioneer Hi-Bred International, Inc. Methods for the identification and characterization of double-strand break sites and compositions and uses thereof
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes

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