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MX2015007039A - Metodos y materiales para la biosintesis de compuestos mogrosidos. - Google Patents

Metodos y materiales para la biosintesis de compuestos mogrosidos.

Info

Publication number
MX2015007039A
MX2015007039A MX2015007039A MX2015007039A MX2015007039A MX 2015007039 A MX2015007039 A MX 2015007039A MX 2015007039 A MX2015007039 A MX 2015007039A MX 2015007039 A MX2015007039 A MX 2015007039A MX 2015007039 A MX2015007039 A MX 2015007039A
Authority
MX
Mexico
Prior art keywords
methods
biosynthesis
materials
useful
mogroside compounds
Prior art date
Application number
MX2015007039A
Other languages
English (en)
Other versions
MX367859B (es
Inventor
Yaoquan Liu
Jørgen Hansen
Jens Houghton-Larsen
Muthuswamy Panchapagesa Murali
Sathish Kumar
Nina Nicoline Rasmussen
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of MX2015007039A publication Critical patent/MX2015007039A/es
Publication of MX367859B publication Critical patent/MX367859B/es

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
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    • C12Y—ENZYMES
    • C12Y303/00—Hydrolases acting on ether bonds (3.3)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La invención se refiere a métodos para producir mogrósidos con la ayuda de enzimas. En particular la invención propone diversas rutas biosintéticas útiles para la producción de mogrósido y se proporcionan enzimas útiles para la producción de mogrósido. Además, la invención proporciona huéspedes recombinantes útiles para realizar los métodos de la invención.
MX2015007039A 2012-12-04 2013-12-04 Metodos y materiales para la biosintesis de compuestos mogrosidos. MX367859B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
PCT/EP2013/075510 WO2014086842A1 (en) 2012-12-04 2013-12-04 Methods and materials for biosynthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
MX2015007039A true MX2015007039A (es) 2016-02-25
MX367859B MX367859B (es) 2019-09-09

Family

ID=49709683

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015007039A MX367859B (es) 2012-12-04 2013-12-04 Metodos y materiales para la biosintesis de compuestos mogrosidos.

Country Status (10)

Country Link
US (4) US9932619B2 (es)
EP (1) EP2929043A1 (es)
JP (1) JP2015536157A (es)
AU (3) AU2015200486A1 (es)
BR (1) BR112015013129A2 (es)
CA (1) CA2893462A1 (es)
HK (1) HK1215053A1 (es)
MX (1) MX367859B (es)
SG (2) SG11201503757RA (es)
WO (1) WO2014086842A1 (es)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014CN03557A (es) 2011-11-23 2015-10-09 Evolva Sa
HK1215053A1 (zh) 2012-12-04 2016-08-12 Evolva Sa 用作生物合成罗汉果化合物的方法及材料
US20170283844A1 (en) * 2014-09-11 2017-10-05 The State of Israel, Ministry of Agriculture & Rural Development, Argricultural Research Organiza Methods of producing mogrosides and compositions comprising same and uses thereof
EP3201315A2 (en) 2014-10-01 2017-08-09 Evolva SA Methods and materials for biosynthesis of mogroside compounds
US10407706B2 (en) 2014-10-17 2019-09-10 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CN104988167A (zh) * 2014-11-21 2015-10-21 中国科学院天津工业生物技术研究所 罗汉果葫芦二烯醇合成酶基因SgCbQ及其应用
CN105801658B (zh) * 2014-12-30 2019-03-19 北京农学院 甜甙及其类似物的制备及作为stat3、erk信号通路靶位点药物在抗肿瘤中的应用
CN106350564A (zh) * 2015-07-20 2017-01-25 中国科学院天津工业生物技术研究所 罗汉果醇合成途径中萜类化合物及其糖基化产物的合成方法
US11357246B2 (en) 2015-10-29 2022-06-14 Firmenich Incorporated High intensity sweeteners
CN105713060A (zh) * 2016-01-29 2016-06-29 滁州润海甜叶菊高科有限公司 一种罗汉果中提取糖分工艺
CN105713061A (zh) * 2016-03-03 2016-06-29 湖南绿蔓生物科技股份有限公司 一种罗汉果苷v含量≥95％的罗汉果提取物的制备方法
CN105777837A (zh) * 2016-05-06 2016-07-20 深圳以诺生物制药有限公司 一种新型罗汉果醇衍生物单体
JP2019519212A (ja) * 2016-05-16 2019-07-11 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
US20200181671A1 (en) * 2017-03-01 2020-06-11 Guilin Layn Natural Ingredients Corp Method for Increasing Content of Mogroside V in Siraitia Grosvenorii Suspended Cells
CN111108213B (zh) 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
RU2762719C2 (ru) 2017-06-02 2021-12-22 Живодан Са Композиция, модифицирующая сладость
US11248248B2 (en) 2017-06-15 2022-02-15 Evolva Sa Production of mogroside compounds in recombinant hosts
CN109385377A (zh) * 2017-08-04 2019-02-26 中国科学院天津工业生物技术研究所 用于生产葫芦二烯醇的工程菌及其制备方法与应用
EP3759230A4 (en) * 2018-02-27 2022-05-25 Manus Bio Inc. MICROBIAL PRODUCTION OF TRITERPENOIDS INCLUDING MOGROSIDES
JP6814182B2 (ja) * 2018-09-10 2021-01-13 アナリティコン ディスカバリー ゲーエムベーハー 甘味料または甘味増強剤として使用される新規なトリペルテングリコシド
CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
CN113286523A (zh) 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
JP2022512931A (ja) * 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
EP3877519A4 (en) 2018-11-09 2022-08-24 Ginkgo Bioworks, Inc. BIOSYNTHESIS OF MOGROSIDS
CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
SG11202109278YA (en) * 2019-02-26 2021-09-29 Coca Cola Co Novel mogrosides and uses of the same
BR112021026302A2 (pt) * 2019-06-25 2022-03-03 Univ California Método para fazer um triterpeno oxidado a partir de beta-amirina, e, célula microbiana engenheirada para fazer um triterpeno oxidado
CN112194568B (zh) * 2019-07-08 2023-04-14 福建中医药大学 一种长链三萜类化合物及其制备方法和用途
CN110283224B (zh) * 2019-07-16 2020-08-28 湖南华诚生物资源股份有限公司 一种罗汉果甜苷v的半合成方法
EP4077649A4 (en) * 2019-12-16 2024-07-10 Manus Bio Inc. MICROBIAL PRODUCTION OF MOGROL AND MOGROSIDES
CN115605081A (zh) * 2020-03-17 2023-01-13 可口可乐公司（Us） 新的罗汉果苷生产系统和方法
EP4138574A1 (en) 2020-04-20 2023-03-01 Givaudan SA Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112903835B (zh) * 2021-01-12 2022-08-12 广州泽力医药科技有限公司 罗汉果橘红固体饮料的指纹图谱构建方法及检测方法
EP4314272A1 (en) * 2021-04-02 2024-02-07 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
KR20240005708A (ko) * 2021-04-02 2024-01-12 징코 바이오웍스, 인크. 이소프레노이드 및 그의 전구체의 생합성
US20240240222A1 (en) 2021-06-29 2024-07-18 Firmenich Incorporated Methods for making high intensity sweeteners
WO2023278226A1 (en) * 2021-06-29 2023-01-05 Firmenich Incorporated Mogroside compounds and their comestible use
CN113755355A (zh) * 2021-09-30 2021-12-07 河北维达康生物科技有限公司 一种以葡萄糖为底物生物合成罗汉果醇的工程菌株、构建及其应用
TWI843509B (zh) * 2022-03-31 2024-05-21 國立臺灣大學 製備三萜類化合物的方法
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法
WO2025144785A2 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US7569389B2 (en) * 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
MX2007007040A (es) * 2004-12-17 2008-10-24 Metanomics Gmbh Proceso para el control de produccion de productos quimicos finos.
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2008122901A (ru) 2005-11-23 2009-12-27 Дзе Кока-Кола Компани (US) Композиция интенсивного подсластителя с сапонином и подслащенные ею композиции
US7927851B2 (en) * 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008062165A2 (en) 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
EP2594646A3 (en) * 2007-09-21 2013-11-06 BASF Plant Science GmbH Plant with increased yield
GB0904639D0 (en) 2009-03-18 2009-04-29 Univ York Assay
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
IN2014CN03557A (es) * 2011-11-23 2015-10-09 Evolva Sa
HK1215053A1 (zh) 2012-12-04 2016-08-12 Evolva Sa 用作生物合成罗汉果化合物的方法及材料

Also Published As

Publication number Publication date
EP2929043A1 (en) 2015-10-14
CA2893462A1 (en) 2014-06-12
US20180346953A1 (en) 2018-12-06
SG10201704575XA (en) 2017-07-28
US20180251806A1 (en) 2018-09-06
US10465222B2 (en) 2019-11-05
US10011859B2 (en) 2018-07-03
WO2014086842A1 (en) 2014-06-12
MX367859B (es) 2019-09-09
WO2014086842A9 (en) 2015-06-18
AU2015200486A1 (en) 2015-03-05
AU2019250137A1 (en) 2019-10-31
US9932619B2 (en) 2018-04-03
US20150064743A1 (en) 2015-03-05
AU2022204430A1 (en) 2022-07-14
SG11201503757RA (en) 2015-06-29
JP2015536157A (ja) 2015-12-21
AU2019250137B2 (en) 2022-03-24
HK1215053A1 (zh) 2016-08-12
US10662458B2 (en) 2020-05-26
BR112015013129A2 (pt) 2017-07-11
US20150322473A1 (en) 2015-11-12

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