[go: up one dir, main page]

MX2014013333A - Variantes de enzima quimosina mejoradas. - Google Patents

Variantes de enzima quimosina mejoradas.

Info

Publication number
MX2014013333A
MX2014013333A MX2014013333A MX2014013333A MX2014013333A MX 2014013333 A MX2014013333 A MX 2014013333A MX 2014013333 A MX2014013333 A MX 2014013333A MX 2014013333 A MX2014013333 A MX 2014013333A MX 2014013333 A MX2014013333 A MX 2014013333A
Authority
MX
Mexico
Prior art keywords
chymosine
improved
variant
enzyme variants
polypeptide
Prior art date
Application number
MX2014013333A
Other languages
English (en)
Other versions
MX357563B (es
Inventor
Petrus Jacobus Theodorus Dekker
René Marcel Jong De
Cornelis Marinus Muijlwijk
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of MX2014013333A publication Critical patent/MX2014013333A/es
Publication of MX357563B publication Critical patent/MX357563B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/0684—Soft uncured Italian cheeses, e.g. Mozarella, Ricotta, Pasta filata cheese; Other similar stretched cheeses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/072—Cheddar type or similar hard cheeses without eyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C2220/00—Biochemical treatment
    • A23C2220/20—Treatment with microorganisms
    • A23C2220/202—Genetic engineering of microorganisms used in dairy technology
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C2250/00—Particular aspects related to cheese
    • A23C2250/15—Shredded non-dried cheese

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Dairy Products (AREA)

Abstract

Un polipéptido variante que tiene actividad quimosina, en que la variante tiene una secuencia de aminoácidos que, cuando se alinea con la quimosina que comprende la secuencia expuesta en SEC ID N°: 2, comprende al menos una sustitución de un resto de aminoácido correspondiente a cualquiera de los aminoácidos 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 o 295 definiéndose dichas posiciones con referencia a SEC ID N°: 2 y en que la variante tiene una o más propiedades alteradas en comparación con un polipéptido de referencia que tiene actividad quimosina. Dicho polipéptido variante puede usarse en la preparación de un queso.
MX2014013333A 2012-05-03 2013-05-03 Variantes de enzima quimosina mejoradas. MX357563B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261642095P 2012-05-03 2012-05-03
EP12166673 2012-05-03
US201261745063P 2012-12-21 2012-12-21
EP12199277 2012-12-21
EP12199178 2012-12-21
PCT/EP2013/059315 WO2013164479A2 (en) 2012-05-03 2013-05-03 Improved enzyme variants

Publications (2)

Publication Number Publication Date
MX2014013333A true MX2014013333A (es) 2015-02-10
MX357563B MX357563B (es) 2018-07-13

Family

ID=49514227

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014013333A MX357563B (es) 2012-05-03 2013-05-03 Variantes de enzima quimosina mejoradas.
MX2014013329A MX355170B (es) 2012-05-03 2013-05-03 Variantes de enzimas mejoradas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2014013329A MX355170B (es) 2012-05-03 2013-05-03 Variantes de enzimas mejoradas.

Country Status (12)

Country Link
US (3) US10167463B2 (es)
EP (3) EP2844751B1 (es)
AU (2) AU2013255747A1 (es)
BR (2) BR112014027371A2 (es)
CA (2) CA2872132C (es)
DK (2) DK2844751T3 (es)
EA (2) EA038324B1 (es)
HK (2) HK1207881A1 (es)
LT (2) LT2844751T (es)
MX (2) MX357563B (es)
NZ (1) NZ701125A (es)
WO (2) WO2013164479A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3929288A1 (en) * 2012-05-25 2021-12-29 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
EP3110948B1 (en) * 2014-02-26 2025-04-09 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
UA125925C2 (uk) 2015-02-10 2022-07-13 Кр. Гансен А/С Композиція для згортання молока, що містить суміш хімозинів, харчова та кормова композиції, що містять суміш хімозинів, застосування композиції для згортання молока, спосіб одержання продукту на основі молока та спосіб одержання ферментованого молочного продукту з використанням композиції для згортання молока
DK3310914T3 (da) 2015-06-22 2022-06-20 Chr Hansen As Chymosinvarianter med forbedrede egenskaber
CA2995755A1 (en) * 2015-08-31 2017-03-09 Chr. Hansen A/S Variants of chymosin with improved properties
EP3143879B1 (en) 2015-09-18 2025-01-22 DSM IP Assets B.V. Yogurt with reduced ripening
GB201517601D0 (en) * 2015-10-06 2015-11-18 Dale Farm Ltd Improvements relating to cheese making
WO2017198829A1 (en) 2016-05-19 2017-11-23 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
BR112018073261B1 (pt) * 2016-05-19 2023-04-25 Chr. Hansen A/S Variante de polipeptídeo quimosina isolada, seu método de produção e seu uso, método para produção de um alimento ou produto alimentício
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
CN111601509A (zh) * 2018-01-11 2020-08-28 科·汉森有限公司 提高干酪产量的培养物、促凝剂和技术之间的相互作用
CN109182311B (zh) * 2018-10-11 2021-07-27 山东隆科特酶制剂有限公司 一种用于沂蒙黑山羊乳奶酪的凝乳酶
EP4373285A1 (en) 2021-07-20 2024-05-29 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
CA3245506A1 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S ASPARTIC PROTEASE, PROCESSES AND ASSOCIATED USES
EP4680032A1 (en) 2023-03-15 2026-01-21 DSM IP Assets B.V. Novel process for producing a fermented milk product and new fermented milk product so produced
WO2025099224A1 (en) 2023-11-08 2025-05-15 Dsm Ip Assets B.V. Improved enzyme variants
WO2025099225A1 (en) 2023-11-08 2025-05-15 Dsm Ip Assets B.V. Improved enzyme variants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR031620A1 (es) * 2000-11-06 2003-09-24 Eidgenoess Tech Hochschule Un metodo para producir quimosina no-bovina y su uso
WO2008008766A2 (en) * 2006-07-10 2008-01-17 Ohio University Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells
EP3763220A1 (en) * 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk

Also Published As

Publication number Publication date
US20180030427A1 (en) 2018-02-01
MX355170B (es) 2018-04-05
MX2014013329A (es) 2015-02-10
BR112014027283A2 (pt) 2017-08-08
HK1207880A1 (en) 2016-02-12
WO2013164479A3 (en) 2013-12-19
EP3450552A2 (en) 2019-03-06
EP2844751B1 (en) 2018-10-17
US10253305B2 (en) 2019-04-09
EP2844751A2 (en) 2015-03-11
AU2013255747A1 (en) 2014-11-06
WO2013164479A2 (en) 2013-11-07
BR112014027371A2 (pt) 2017-08-08
US10167463B2 (en) 2019-01-01
US20150118356A1 (en) 2015-04-30
DK2844750T3 (en) 2018-10-01
LT2844750T (lt) 2018-09-10
LT2844751T (lt) 2019-02-11
WO2013164481A1 (en) 2013-11-07
CA2872132C (en) 2020-06-16
HK1207881A1 (en) 2016-02-12
AU2013255749A1 (en) 2014-11-06
US20150140169A1 (en) 2015-05-21
DK2844751T3 (en) 2019-02-04
EA201401211A1 (ru) 2015-05-29
EP3450552A3 (en) 2019-05-08
EP2844750A1 (en) 2015-03-11
US9822352B2 (en) 2017-11-21
CA2872309C (en) 2021-03-02
CA2872132A1 (en) 2013-11-07
MX357563B (es) 2018-07-13
EA035275B1 (ru) 2020-05-22
NZ701125A (en) 2016-11-25
CA2872309A1 (en) 2013-11-07
EA038324B1 (ru) 2021-08-10
EP2844750B1 (en) 2018-06-20
EA201401209A1 (ru) 2015-05-29

Similar Documents

Publication Publication Date Title
NZ701125A (en) Improved chymosine enzyme variants
PH12014501726A1 (en) Glyphosate resistant plants and associated methods
MX2020010399A (es) 3-epimerasa.
TN2017000085A1 (en) Polypeptide having a polyester degrading activity and uses thereof
MY160995A (en) Polypeptides with permease activity
PH12013502022B1 (en) Lyophilized preparation of botulinum toxin
IN2014DN07017A (es)
MX2020011083A (es) Proteina.
MX340248B (es) Celobiohidrolasa mutante.
MX2015001542A (es) Metodo.
MX2015003310A (es) Moleculas de union il-17a novedosas y usos medicos de las mismas.
WO2013098205A3 (en) Detergent compositions with lipase variants
MX2012009847A (es) Celulasas de familia 5 modificada y usos de las mismas.
NZ757945A (en) Lactase enzymes with improved properties
NZ757950A (en) Lactase enzymes with improved properties
EA201591609A1 (ru) Варианты альфа-амилазы
EP4043558A3 (en) Kaurenoic acid hydroxylases
MY187334A (en) Xylanase
EA033091B1 (ru) ПШЕНИЦА С НОВЫМИ АЛЛЕЛЯМИ Rht-B1
NZ709120A (en) Beta-hexosyl-transferases and uses thereof
AR095027A1 (es) VARIANTES DE a-AMILASA
WO2012031103A3 (en) Inhibitors of bcl-2
MX2014013255A (es) Endoglucanasas con propiedades mejoradas.
CN106687579A8 (zh) 产生二胺的微生物和使用该微生物产生二胺的方法
MX2019007375A (es) Variante de enzima lipolitica.

Legal Events

Date Code Title Description
FG Grant or registration