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AR095027A1 - VARIANTES DE a-AMILASA - Google Patents

VARIANTES DE a-AMILASA

Info

Publication number
AR095027A1
AR095027A1 ARP140100725A ARP140100725A AR095027A1 AR 095027 A1 AR095027 A1 AR 095027A1 AR P140100725 A ARP140100725 A AR P140100725A AR P140100725 A ARP140100725 A AR P140100725A AR 095027 A1 AR095027 A1 AR 095027A1
Authority
AR
Argentina
Prior art keywords
variant
polypeptide
amylase
seq
amino acid
Prior art date
Application number
ARP140100725A
Other languages
English (en)
Inventor
T Van Rij Evert
E Abbas Hanna
M De Jong Rene
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR095027A1 publication Critical patent/AR095027A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un polipéptido variante que tiene actividad a-amilasa, en donde la variante tiene una secuencia de aminoácidos la cual, cuando se alinea con la a-amilasa que comprende la secuencia indicada en SEQ ID Nº 2, comprende al menos una sustitución de un residuo de aminoácido que se corresponde con cualquiera de los aminoácidos 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 554, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677 estando dichas posiciones definidas con referencia a SEQ ID Nº 2 y en donde la variante tiene una o más propiedades alteradas en comparación a un polipéptido de referencia que tiene actividad a-amilasa. Dicho polipéptido variante puede usarse en la preparación de un producto horneado.
ARP140100725A 2013-03-01 2014-03-06 VARIANTES DE a-AMILASA AR095027A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13157398 2013-03-01

Publications (1)

Publication Number Publication Date
AR095027A1 true AR095027A1 (es) 2015-09-16

Family

ID=47750572

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140100725A AR095027A1 (es) 2013-03-01 2014-03-06 VARIANTES DE a-AMILASA

Country Status (2)

Country Link
US (2) US9301533B2 (es)
AR (1) AR095027A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111935981B (zh) * 2018-04-05 2024-06-14 帝斯曼知识产权资产管理有限公司 变体麦芽糖α-淀粉酶
EP4056635A1 (en) 2021-03-12 2022-09-14 Premo, Sa A thermal conductive composition, a thermal conductive potting for sealing a magnetic power assembly, a power transformer assembly and an electrical vehicle
EP4504928A1 (en) * 2022-04-01 2025-02-12 Danstar Ferment AG N-terminus engineering of intracellular polypeptides expressed in recombinant eukaryotic host cells
CN119464255B (zh) * 2024-12-17 2026-01-06 安徽大学 一种特异性水解糊化淀粉的新型α-淀粉酶及应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
USRE38507E1 (en) 1989-09-27 2004-04-27 Novozymes A/S Antistaling process and agent
ATE490312T1 (de) 1998-02-27 2010-12-15 Novozymes As Maltogene alpha-amylase varianten
EP1066374B1 (en) 1998-02-27 2006-05-31 Novozymes A/S Amylolytic enzyme variants
GB0305685D0 (en) 2003-03-12 2003-04-16 Danisco Enzyme
AU2005269082A1 (en) 2004-08-02 2006-02-09 Novozymes A/S Creation of diversity in polypeptides
WO2006012899A1 (en) 2004-08-02 2006-02-09 Novozymes A/S Maltogenic alpha-amylase variants
CA2610683C (en) 2004-09-24 2015-12-22 Novozymes A/S Method of preparing a dough-based product
DK2164958T3 (en) 2007-06-07 2014-11-24 Novozymes As A process for preparing a dough-based product
EP2499227B1 (en) 2009-11-13 2015-04-08 Novozymes A/S A brewing method
DK2620496T3 (en) * 2012-01-30 2015-09-07 Dsm Ip Assets Bv Alpha-amylase

Also Published As

Publication number Publication date
US20150376588A1 (en) 2015-12-31
US20140248397A1 (en) 2014-09-04
US9301533B2 (en) 2016-04-05
US9808018B2 (en) 2017-11-07

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