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MX2013013644A - Peptidos de transito al cloroplasto y metodos de uso. - Google Patents

Peptidos de transito al cloroplasto y metodos de uso.

Info

Publication number
MX2013013644A
MX2013013644A MX2013013644A MX2013013644A MX2013013644A MX 2013013644 A MX2013013644 A MX 2013013644A MX 2013013644 A MX2013013644 A MX 2013013644A MX 2013013644 A MX2013013644 A MX 2013013644A MX 2013013644 A MX2013013644 A MX 2013013644A
Authority
MX
Mexico
Prior art keywords
ctp
methods
chloroplast transit
operably linked
chimeric
Prior art date
Application number
MX2013013644A
Other languages
English (en)
Inventor
Gary C Chun
Timothy M Bourett
Albert L Lu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX2013013644A publication Critical patent/MX2013013644A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se proporcionan métodos y composiciones para dirigir un polipéptido de interés a un cloroplasto. Se proporcionan polinucleótidos recombinantes que comprenden una secuencia de nucleótidos que codifican un péptido de tránsito al cloroplasto (CTP) quimérico unido operativamente a un polinucleótido heterálogo de interés. En modalidades específicas, el CTP quimérico comprende un dominio N-terminal unido operativamente a un dominio central unido operativamente a un dominio C-terminal de un CTP para formar un péptido de tránsito al cloroplasto quimérico que tiene actividad de CTP. Además, se proporcionan polipéptidos recornbinantes que codifican a estos, así como a células, células vegetales, plantas y semillas que comprenden los polinucleótidos recombinantes. Además, se proporcionan métodos de uso de las diversas secuencias.
MX2013013644A 2011-05-23 2012-05-10 Peptidos de transito al cloroplasto y metodos de uso. MX2013013644A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161488952P 2011-05-23 2011-05-23
PCT/US2012/037360 WO2012161982A1 (en) 2011-05-23 2012-05-10 Chloroplast transit peptides and methods of their use

Publications (1)

Publication Number Publication Date
MX2013013644A true MX2013013644A (es) 2013-12-12

Family

ID=46124770

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013013644A MX2013013644A (es) 2011-05-23 2012-05-10 Peptidos de transito al cloroplasto y metodos de uso.

Country Status (7)

Country Link
US (1) US9150625B2 (es)
EP (1) EP2714725A1 (es)
CN (1) CN103748108A (es)
BR (1) BR112013029807A2 (es)
CA (1) CA2836775A1 (es)
MX (1) MX2013013644A (es)
WO (1) WO2012161982A1 (es)

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Also Published As

Publication number Publication date
BR112013029807A2 (pt) 2017-01-24
US20120304336A1 (en) 2012-11-29
WO2012161982A1 (en) 2012-11-29
EP2714725A1 (en) 2014-04-09
CA2836775A1 (en) 2012-11-29
US9150625B2 (en) 2015-10-06
CN103748108A (zh) 2014-04-23

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