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MX2013013224A - Procedimiento de purificacion de proteinas de bajo ph. - Google Patents

Procedimiento de purificacion de proteinas de bajo ph.

Info

Publication number
MX2013013224A
MX2013013224A MX2013013224A MX2013013224A MX2013013224A MX 2013013224 A MX2013013224 A MX 2013013224A MX 2013013224 A MX2013013224 A MX 2013013224A MX 2013013224 A MX2013013224 A MX 2013013224A MX 2013013224 A MX2013013224 A MX 2013013224A
Authority
MX
Mexico
Prior art keywords
bssl
low
purification process
protein purification
hic
Prior art date
Application number
MX2013013224A
Other languages
English (en)
Inventor
Susanne Wood
Original Assignee
Swedish Orphan Biovitrum Ab Publ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Swedish Orphan Biovitrum Ab Publ filed Critical Swedish Orphan Biovitrum Ab Publ
Publication of MX2013013224A publication Critical patent/MX2013013224A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La invención se refiere a un procedimiento para recuperar y purificar lipasa estimulada con sal biliar (BSSL) en una solución que contiene impurezas, dicho procedimiento comprende los pasos: (i) aplicar BSSL a una resina de cromatografía de interacción hidrófoba (HIC); (ii) eliminar las impurezas al lavar dicha resina de HIC con una composición de lavado que tiene un pH en la escala de 4 a 5; y (iii) recuperar la BSSL de dicha resina de HIC.
MX2013013224A 2011-05-18 2012-05-15 Procedimiento de purificacion de proteinas de bajo ph. MX2013013224A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE1150454 2011-05-18
PCT/SE2012/050519 WO2012158109A1 (en) 2011-05-18 2012-05-15 Low ph protein purification process

Publications (1)

Publication Number Publication Date
MX2013013224A true MX2013013224A (es) 2014-04-25

Family

ID=47177197

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013013224A MX2013013224A (es) 2011-05-18 2012-05-15 Procedimiento de purificacion de proteinas de bajo ph.

Country Status (12)

Country Link
US (1) US20140186921A1 (es)
EP (1) EP2710126A4 (es)
JP (1) JP2014514932A (es)
KR (1) KR20140034223A (es)
CN (1) CN103562383A (es)
AU (1) AU2012256449B2 (es)
CA (1) CA2835407A1 (es)
IL (1) IL229383A0 (es)
MX (1) MX2013013224A (es)
RU (1) RU2013156071A (es)
SG (1) SG194934A1 (es)
WO (1) WO2012158109A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102087854B1 (ko) 2011-06-10 2020-03-12 메르사나 테라퓨틱스, 인코포레이티드 단백질-중합체-약물 접합체
US8815226B2 (en) 2011-06-10 2014-08-26 Mersana Therapeutics, Inc. Protein-polymer-drug conjugates
GB201622343D0 (en) * 2016-12-29 2017-02-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042461A (en) * 1976-09-10 1977-08-16 Eastman Kodak Company Method for purifying cholesterol esterase
US5200183A (en) * 1987-11-19 1993-04-06 Oklahoma Medical Research Foundation Recombinant bile salt activated lipases
AU3434889A (en) * 1988-03-15 1989-10-05 Jewish Hospital Of St. Louis, The Inhibition of intestinal cholesterol and fatty acid absorption
US5173408A (en) * 1989-11-13 1992-12-22 Lange Louis George Iii Mammalian pancreatic cholesterol esterase
US5849874A (en) * 1991-07-12 1998-12-15 Gist-Brocades, N.V. Process for the purification of serum albumin
ES2050068B1 (es) * 1992-07-03 1994-12-16 Consejo Superior Investigacion Procedimiento para la purificacion de dos isoenzimas lipasa de candida rugosa.
IS4130A (is) * 1993-03-01 1994-09-02 Ab Astra Ný fjölpeptíð
SE9801424D0 (sv) * 1998-04-22 1998-04-22 Astra Ab Expression methods
MX2010001612A (es) * 2007-08-09 2010-07-01 Usv Ltd Novedoso procedimiento ortogonal para la purificacion de la hormona paratiroidea humana recombinante (rhpth) (1-34).

Also Published As

Publication number Publication date
IL229383A0 (en) 2014-01-30
SG194934A1 (en) 2013-12-30
EP2710126A1 (en) 2014-03-26
AU2012256449B2 (en) 2015-04-09
CA2835407A1 (en) 2012-11-22
KR20140034223A (ko) 2014-03-19
RU2013156071A (ru) 2015-06-27
CN103562383A (zh) 2014-02-05
US20140186921A1 (en) 2014-07-03
AU2012256449A1 (en) 2013-11-07
WO2012158109A1 (en) 2012-11-22
EP2710126A4 (en) 2014-11-26
JP2014514932A (ja) 2014-06-26

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Legal Events

Date Code Title Description
FA Abandonment or withdrawal