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MX2013012153A - Variantes de fitasa sintetica. - Google Patents

Variantes de fitasa sintetica.

Info

Publication number
MX2013012153A
MX2013012153A MX2013012153A MX2013012153A MX2013012153A MX 2013012153 A MX2013012153 A MX 2013012153A MX 2013012153 A MX2013012153 A MX 2013012153A MX 2013012153 A MX2013012153 A MX 2013012153A MX 2013012153 A MX2013012153 A MX 2013012153A
Authority
MX
Mexico
Prior art keywords
synthetic phytase
phytase
synthetic
elevated
phytase variants
Prior art date
Application number
MX2013012153A
Other languages
English (en)
Inventor
Stefan Haefner
Annegret Welzel
Robert Thummer
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of MX2013012153A publication Critical patent/MX2013012153A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Detergent Compositions (AREA)
  • Fertilizers (AREA)

Abstract

La invención se refiere a una fitasa sintética con alto grado de termoestabiIidad alto grado de estabilidad frente a ácidos con pH 2, alto grado de estabilidad frente a la pepsina y con un intervalo ampliado de pH activo, y a una secuencia aislada de ácido nucleico que codifica una fitasa sintética y al uso de la fitasa en alimentos para animales con el fin de reducir el contenido de fosfato en el abono natural líquido, y a los aditivos para alimentos para animales y alimentos para animales que contienen la fitasa sintética.
MX2013012153A 2011-04-21 2012-04-18 Variantes de fitasa sintetica. MX2013012153A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161477645P 2011-04-21 2011-04-21
EP11163453 2011-04-21
PCT/IB2012/051930 WO2012143861A1 (en) 2011-04-21 2012-04-18 Synthetic phytase variants

Publications (1)

Publication Number Publication Date
MX2013012153A true MX2013012153A (es) 2013-12-10

Family

ID=47041110

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013012153A MX2013012153A (es) 2011-04-21 2012-04-18 Variantes de fitasa sintetica.

Country Status (14)

Country Link
US (1) US9127260B2 (es)
EP (1) EP2699674B1 (es)
JP (1) JP5944485B2 (es)
KR (1) KR101692103B1 (es)
CN (1) CN103687944B (es)
AR (1) AR086033A1 (es)
BR (1) BR112013026496A2 (es)
CA (1) CA2831378A1 (es)
DK (1) DK2699674T3 (es)
ES (1) ES2562654T3 (es)
HU (1) HUE027001T2 (es)
MX (1) MX2013012153A (es)
PL (1) PL2699674T3 (es)
WO (1) WO2012143861A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2699675T3 (en) * 2011-04-21 2015-11-30 Basf Se Synthetic fytasevarianter
CN106455630B (zh) 2014-06-27 2021-08-24 帝斯曼知识产权资产管理有限公司 改进动物饲料的营养价值的方法
CN106282081B (zh) * 2016-08-31 2020-02-11 江南大学 一种高产维生素c-2-磷酸酯的方法
CN107488642B (zh) * 2017-09-30 2020-08-18 山东隆科特酶制剂有限公司 一种植酸酶突变体及其应用
CN113301809B (zh) * 2018-11-20 2024-08-02 国际N&H丹麦有限公司 工程化的稳健的高Tm植酸酶进化枝多肽及其片段
CN113717959B (zh) * 2020-05-22 2024-04-02 青岛蔚蓝生物集团有限公司 植酸酶突变体
EP4001407A1 (en) * 2020-11-17 2022-05-25 EW Nutrition GmbH Stable synthetic phytase variants
DE102020131856A1 (de) * 2020-12-01 2022-06-02 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Körperschaft des öffentlichen Rechts Thermostabile Phytase-Chimära
CN114807088B (zh) * 2022-06-28 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种提高植酸酶热稳定性的方法及突变体APPAmut6和应用
WO2024124013A1 (en) * 2022-12-09 2024-06-13 International N&H Denmark Aps Feed formulations comprising a phytase for dairy ruminant animals
EP4637384A1 (en) * 2022-12-23 2025-10-29 Basf Se Phytase variants with improved properties
KR20240139174A (ko) * 2023-03-13 2024-09-23 씨제이제일제당 (주) 피타아제 변이체
WO2025132617A1 (en) * 2023-12-18 2025-06-26 Ew Nutrition Gmbh Synthetic phytase variants

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ289014B6 (cs) 1989-09-27 2001-10-17 Dsm N. V. Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy
ATE238425T1 (de) 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
PL336345A1 (en) 1997-04-11 2000-06-19 Dsm Nv Genic conversion as a tool for constructing recombined filiform fungi
AU2002230953A1 (en) 2000-12-12 2002-06-24 Diversa Corporation Recombinant phytases and uses thereof
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
WO2007112739A1 (en) 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
WO2007128160A1 (en) 2006-04-30 2007-11-15 Feed Research Institute Chinese Academy Of Agricultural Sciences Cloning and expression of a novel phytase
US8371791B2 (en) 2006-07-28 2013-02-12 Gerard F. Kenna Hay and silage feeder
CA2676649C (en) 2007-01-30 2016-08-30 Novozymes A/S Polypeptides having phytase activty and polynucleotides encoding same
US20080220498A1 (en) 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
JP5463146B2 (ja) 2007-02-07 2014-04-09 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン アルファ‐アミラーゼを有するフィターゼを用いるデンプン加水分解
JP5643083B2 (ja) * 2007-03-26 2014-12-17 ノボザイムス アクティーゼルスカブ ハフニア・フィターゼ
DK2342323T3 (da) 2008-09-26 2013-09-08 Novozymes As Hafnia phytasevarianter
JP2013507939A (ja) * 2009-10-22 2013-03-07 ビーエーエスエフ ソシエタス・ヨーロピア 合成フィターゼ変異体
DK2699675T3 (en) * 2011-04-21 2015-11-30 Basf Se Synthetic fytasevarianter

Also Published As

Publication number Publication date
AR086033A1 (es) 2013-11-13
DK2699674T3 (da) 2016-03-21
KR101692103B1 (ko) 2017-01-02
PL2699674T3 (pl) 2016-06-30
JP2014513944A (ja) 2014-06-19
ES2562654T3 (es) 2016-03-07
CN103687944A (zh) 2014-03-26
BR112013026496A2 (pt) 2016-11-29
CN103687944B (zh) 2016-08-17
US20140044835A1 (en) 2014-02-13
US9127260B2 (en) 2015-09-08
JP5944485B2 (ja) 2016-07-05
CA2831378A1 (en) 2012-10-26
EP2699674A1 (en) 2014-02-26
EP2699674B1 (en) 2016-01-06
HUE027001T2 (en) 2016-10-28
KR20140009514A (ko) 2014-01-22
EP2699674A4 (en) 2014-12-17
WO2012143861A1 (en) 2012-10-26

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