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MX2013011737A - Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido. - Google Patents

Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido.

Info

Publication number
MX2013011737A
MX2013011737A MX2013011737A MX2013011737A MX2013011737A MX 2013011737 A MX2013011737 A MX 2013011737A MX 2013011737 A MX2013011737 A MX 2013011737A MX 2013011737 A MX2013011737 A MX 2013011737A MX 2013011737 A MX2013011737 A MX 2013011737A
Authority
MX
Mexico
Prior art keywords
nucleic acid
tissue sample
array
capture
domain
Prior art date
Application number
MX2013011737A
Other languages
English (en)
Other versions
MX340330B (es
Inventor
Jonas Frisen
Patrik Ståhl
Joakim Lundeberg
Original Assignee
Spatial Transcriptomics Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44123034&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2013011737(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Spatial Transcriptomics Ab filed Critical Spatial Transcriptomics Ab
Publication of MX2013011737A publication Critical patent/MX2013011737A/es
Publication of MX340330B publication Critical patent/MX340330B/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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Abstract

La presente invención se refiere a métodos y productos para la detección localizada o espacial de ácido nucleico en una muestra de tejido y, en particular, a un método para la detección localizada de ácido nucleico en una muestra de tejido que comprende: (a) proporcionar una matriz que comprende un sustrato en el cual múltiples especies de sondas de captura son directa o indirectamente inmovilizadas de tal manera que cada especie ocupa una posición distinta en la matriz y está orientada para tener un extremo 3' libre para hacer posible que la sonda funcione como un cebador para una reacción de extensión o ligación de cebador, en donde cada especie de la sonda de captura comprende una molécula de ácido nucleico con 5´ a 3´ : (i) un dominio posicional que corresponde a la posición de la sonda de captura en la matriz, y (ii) un dominio de captura; (b) poner en contacto la matriz con una muestra de tejido de tal manera que la posición de una sonda de captura en la matriz puede estar correlacionada con una posición en la muestra de tejido y permitir que el ácido nucleico de la muestra de tejido se hibride al dominio de captura en la sonda de captura; (c) generar moléculas de ADN de moléculas de ácido nucleico capturadas utilizando sondas de captura como cebadores de extensión o ligación, en donde las moléculas de ADN extendidas y ligadas se etiquetan en virtud del dominio posicional; (d) opcionalmente generar una hebra complementaria del ADN etiquetado y/u opcionalmente amplificar el ADN etiquetado; (e) liberar al menos parte de las moléculas de ADN etiquetadas y/o sus complementos o amplicones de la superficie de la matriz, en donde la parte incluye el dominio posicional o un complemento del mismo; y (f) analizar directa o indirectamente la secuencia de las moléculas de ADN liberadas.
MX2013011737A 2011-04-13 2012-04-13 Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido. MX340330B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1106254.4A GB201106254D0 (en) 2011-04-13 2011-04-13 Method and product
PCT/EP2012/056823 WO2012140224A1 (en) 2011-04-13 2012-04-13 Method and product for localised or spatial detection of nucleic acid in a tissue sample

Publications (2)

Publication Number Publication Date
MX2013011737A true MX2013011737A (es) 2014-03-27
MX340330B MX340330B (es) 2016-07-06

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MX2013011737A MX340330B (es) 2011-04-13 2012-04-13 Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido.

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Country Link
US (15) US10030261B2 (es)
EP (4) EP4206333A1 (es)
JP (1) JP5916166B2 (es)
KR (1) KR101994494B1 (es)
CN (3) CN115896252A (es)
AU (1) AU2012241730B2 (es)
BR (1) BR112013026502A2 (es)
CA (1) CA2832678C (es)
GB (1) GB201106254D0 (es)
MX (1) MX340330B (es)
NZ (1) NZ616407A (es)
RU (1) RU2603074C2 (es)
WO (1) WO2012140224A1 (es)

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