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MX2012015025A - Método para reacción en cadena de polimerasa (pcr) directa utilizando un material polimérico como portador de muestra. - Google Patents

Método para reacción en cadena de polimerasa (pcr) directa utilizando un material polimérico como portador de muestra.

Info

Publication number
MX2012015025A
MX2012015025A MX2012015025A MX2012015025A MX2012015025A MX 2012015025 A MX2012015025 A MX 2012015025A MX 2012015025 A MX2012015025 A MX 2012015025A MX 2012015025 A MX2012015025 A MX 2012015025A MX 2012015025 A MX2012015025 A MX 2012015025A
Authority
MX
Mexico
Prior art keywords
nucleic acid
polymeric material
sampling device
methods
containing material
Prior art date
Application number
MX2012015025A
Other languages
English (en)
Other versions
MX340926B (es
Inventor
Paul Gerald Debenham
David John Moore
Original Assignee
Lgc Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lgc Ltd filed Critical Lgc Ltd
Publication of MX2012015025A publication Critical patent/MX2012015025A/es
Publication of MX340926B publication Critical patent/MX340926B/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N2001/002Devices for supplying or distributing samples to an analysing apparatus
    • G01N2001/007Devices specially adapted for forensic samples, e.g. tamper-proofing, sample tracking
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N2001/028Sampling from a surface, swabbing, vaporising

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un método para la amplificación de ácido nucleico cuya fuente de ácido nucleico es una eucariota superior, tal como un mamífero o una planta, según un método con las etapas (A) poner en contacto un dispositivo de muestreo con la fuente de ácido nucleico del eucariota superior, tal como un mamífero o planta, de tal manera que después de dicho contacto, material que contiene ácido nucleico del eucariota superior, tal como un mamífero o planta, se adhiere a al menos una parte del dispositivo de muestreo, donde el dispositivo de muestreo, o la parte del mismo a la que se encuentra adherida el material que contiene el ácido nucleico, está hecha de un material polimérico; (B) introducir el dispositivo de muestreo o parte del mismo a la que el material que contiene ácido nucleico se adhiere en un recipiente de reacción que contiene una mezcla de reacción para llevar a cabo una reacción amplificación de ácido nucleico, sin ningún tratamiento previo al material que contiene el ácido nucleico; y (C) realizar una reacción de amplificación de ácido nucleico.
MX2012015025A 2010-06-18 2011-06-17 Método para reacción en cadena de polimerasa (pcr) directa utilizando un material polimérico como portador de muestra. MX340926B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201010237A GB201010237D0 (en) 2010-06-18 2010-06-18 Methods and apparatuses
PCT/GB2011/051133 WO2011158037A2 (en) 2010-06-18 2011-06-17 Methods and apparatuses

Publications (2)

Publication Number Publication Date
MX2012015025A true MX2012015025A (es) 2013-04-03
MX340926B MX340926B (es) 2016-08-01

Family

ID=42471855

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012015025A MX340926B (es) 2010-06-18 2011-06-17 Método para reacción en cadena de polimerasa (pcr) directa utilizando un material polimérico como portador de muestra.

Country Status (14)

Country Link
US (1) US9139871B2 (es)
EP (1) EP2582843B1 (es)
JP (1) JP5766284B2 (es)
CN (1) CN103069008B (es)
AU (1) AU2011266792B2 (es)
BR (1) BR112012032369A2 (es)
CA (1) CA2802911A1 (es)
ES (1) ES2578263T3 (es)
GB (2) GB201010237D0 (es)
MX (1) MX340926B (es)
NZ (2) NZ700170A (es)
RU (1) RU2549694C2 (es)
WO (1) WO2011158037A2 (es)
ZA (1) ZA201209615B (es)

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JP6652673B1 (ja) * 2019-06-07 2020-02-26 日本板硝子株式会社 反応処理容器
CN111549024B (zh) * 2020-05-09 2023-06-30 浙江省中药研究所有限公司 一种核酸提取试纸条及其使用方法
RU2770918C1 (ru) * 2021-06-07 2022-04-25 Федеральное Государственное Казенное Образовательное Учреждение Высшего Образования «Санкт-Петербургская Академия Следственного Комитета Российской Федерации» Способ изъятия биологических следов со снега и устройство для его реализации

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Also Published As

Publication number Publication date
AU2011266792B2 (en) 2014-11-06
GB201205470D0 (en) 2012-05-09
ZA201209615B (en) 2014-05-28
NZ700170A (en) 2016-01-29
GB2485955B (en) 2013-04-24
AU2011266792A1 (en) 2013-01-10
WO2011158037A3 (en) 2012-03-29
CA2802911A1 (en) 2011-12-22
MX340926B (es) 2016-08-01
EP2582843A2 (en) 2013-04-24
JP2013528393A (ja) 2013-07-11
NZ604683A (en) 2014-10-31
CN103069008B (zh) 2016-01-20
US20130157315A1 (en) 2013-06-20
JP5766284B2 (ja) 2015-08-19
ES2578263T3 (es) 2016-07-22
CN103069008A (zh) 2013-04-24
GB2485955A (en) 2012-05-30
HK1171476A1 (en) 2013-03-28
EP2582843B1 (en) 2016-03-30
GB201010237D0 (en) 2010-07-21
RU2013102241A (ru) 2014-07-27
WO2011158037A2 (en) 2011-12-22
BR112012032369A2 (pt) 2016-10-04
US9139871B2 (en) 2015-09-22
RU2549694C2 (ru) 2015-04-27

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