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MX2012001854A - Ensayo para realizar una evaluacion de riesgo molecular de una muestra microbiana compleja sospechosa de contener una ehec. - Google Patents

Ensayo para realizar una evaluacion de riesgo molecular de una muestra microbiana compleja sospechosa de contener una ehec.

Info

Publication number
MX2012001854A
MX2012001854A MX2012001854A MX2012001854A MX2012001854A MX 2012001854 A MX2012001854 A MX 2012001854A MX 2012001854 A MX2012001854 A MX 2012001854A MX 2012001854 A MX2012001854 A MX 2012001854A MX 2012001854 A MX2012001854 A MX 2012001854A
Authority
MX
Mexico
Prior art keywords
target genes
ehec
contain
risk assessment
sample suspected
Prior art date
Application number
MX2012001854A
Other languages
English (en)
Other versions
MX340945B (es
Inventor
Patrick Fach
Marie Bugarel
Lothar Beutin
Original Assignee
Agente Nationale De Securite Sanitaire De L Alimentation De L Environnement Et Du Travail
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agente Nationale De Securite Sanitaire De L Alimentation De L Environnement Et Du Travail filed Critical Agente Nationale De Securite Sanitaire De L Alimentation De L Environnement Et Du Travail
Publication of MX2012001854A publication Critical patent/MX2012001854A/es
Publication of MX340945B publication Critical patent/MX340945B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un proceso para realizar una evaluación de riesgo molecular (MRA) en una muestra sospechosa de contener una Escherichia coli (EHEC), que comprende los pasos: poner en contacto dicha muestra o ADN aislado de la misma con un par de cebadores derivados de los siguientes genes objetivo stxl, stx2, eae y/o espk; en donde el proceso se caracteriza porque si se detectan productos de amplificación para cada uno de los genes objetivo en el primer paso, en un segundo paso dicha muestra o ADN aislado de la misma se pone en contacto con un par de cebadores derivados de los siguientes genes objetivo nleB, nIeH1-2, nIeE, ent/espL2, eae subtipos y, ß ( y ? y los genes objetivo rfbE (0157), wbdl (0111), wzx (026); ihpl (0145), wzx (0103); y detectar la presencia o la ausencia de un producto de amplificación para cada uno de los genes objetivo.
MX2012001854A 2009-08-11 2010-08-11 Ensayo para realizar una evaluacion de riesgo molecular de una muestra microbiana compleja sospechosa de contener una ehec. MX340945B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09290621A EP2292799A1 (en) 2009-08-11 2009-08-11 An assay for determining a molecular risk assessment of stec isolates
PCT/IB2010/053631 WO2011018762A1 (en) 2009-08-11 2010-08-11 An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec

Publications (2)

Publication Number Publication Date
MX2012001854A true MX2012001854A (es) 2012-05-22
MX340945B MX340945B (es) 2016-08-01

Family

ID=41479602

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012001854A MX340945B (es) 2009-08-11 2010-08-11 Ensayo para realizar una evaluacion de riesgo molecular de una muestra microbiana compleja sospechosa de contener una ehec.

Country Status (11)

Country Link
US (1) US9416425B2 (es)
EP (2) EP2292799A1 (es)
JP (1) JP5990099B2 (es)
KR (1) KR101837781B1 (es)
AU (1) AU2010283452B2 (es)
BR (1) BR112012003122B1 (es)
CA (1) CA2770275C (es)
ES (1) ES2614811T3 (es)
MX (1) MX340945B (es)
NZ (1) NZ598008A (es)
WO (1) WO2011018762A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102260743B (zh) * 2011-07-13 2013-03-06 浙江省疾病预防控制中心 肠出血性大肠杆菌o104:h4多重荧光pcr检测试剂盒
JP5979657B2 (ja) * 2011-08-16 2016-08-24 国立大学法人 東京大学 食中毒原因大腸菌検出用プライマー及び検出用キット
PL218839B1 (pl) 2011-09-09 2015-01-30 3G Therapeutics Inc Sposób wykrywania enterokrwotocznych Escherichia coli (EHEC), sonda do wykrywania enterokrwotocznych Escherichia coli (EHEC), sekwencje do amplifikacji fragmentu genu kodującego toksynę Shiga, zastosowanie sond i sekwencji
JP2014530011A (ja) 2011-09-28 2014-11-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Ｓｔｅｃ細菌の配列ならびに検出および特徴決定のためのそれらの使用
CA2862395A1 (en) * 2011-12-30 2013-07-04 Roka Bioscience, Inc. Monitoring shiga toxin producing escherichia coli
EP2674501A1 (en) * 2012-06-14 2013-12-18 Agence nationale de sécurité sanitaire de l'alimentation,de l'environnement et du travail Method for detecting and identifying enterohemorrhagic Escherichia coli
CA2916518A1 (en) * 2013-07-03 2015-01-08 Roka Bioscience, Inc. Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli
EP2843057B1 (en) * 2013-09-03 2017-12-27 Pall Genedisc Technologies Method for determining the presence or absence of shiga toxin-producing Escherichia coli (STEC) in a food sample

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2777907B1 (fr) 1998-04-28 2002-08-30 Pasteur Sanofi Diagnostics Sequences nucleotidiques pour la detection des escherichia coli enterohemorragiques (ehec)
US6291168B1 (en) * 1998-10-27 2001-09-18 Auburn University Nucleic acid sequences diagnostic for pathogenic E.coli O157:H7, methods of identification and kit therefore
US20060094034A1 (en) * 2003-04-30 2006-05-04 Roland Brousseau Virulence and antibiotic resistance array and uses thereof
EP2383287A1 (en) * 2003-10-31 2011-11-02 The University of British Columbia Citrobacter rodentium secreted proteins
US7323308B2 (en) * 2004-09-03 2008-01-29 Affymetrix, Inc. Methods of genetic analysis of E. coli
CN100441696C (zh) 2004-10-22 2008-12-10 中国疾病预防控制中心传染病预防控制所 肠出血性大肠杆菌o157：h7菌株的检测方法

Also Published As

Publication number Publication date
MX340945B (es) 2016-08-01
BR112012003122A2 (pt) 2016-11-22
JP5990099B2 (ja) 2016-09-07
KR101837781B1 (ko) 2018-03-12
CA2770275A1 (en) 2011-02-17
NZ598008A (en) 2014-09-26
EP2464746B1 (en) 2016-11-30
US9416425B2 (en) 2016-08-16
US20120202204A1 (en) 2012-08-09
AU2010283452A1 (en) 2012-03-01
BR112012003122B1 (pt) 2019-10-29
EP2292799A1 (en) 2011-03-09
KR20120100890A (ko) 2012-09-12
CA2770275C (en) 2020-03-31
EP2464746A1 (en) 2012-06-20
JP2013501516A (ja) 2013-01-17
ES2614811T3 (es) 2017-06-02
AU2010283452B2 (en) 2016-03-17
WO2011018762A1 (en) 2011-02-17

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