CA2770275C - An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec - Google Patents

An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec Download PDF

Info

Publication number: CA2770275C
Authority: CA; Canada
Prior art keywords: seq; fragment; eae; pair; fifteen nucleotides
Prior art date: 2009-08-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

CA2770275A

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English (en)

French (fr)

Other versions

CA2770275A1 (en

Inventor

Patrick Fach

Marie Bugarel

Lothar Beutin

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail

Original Assignee

Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2009-08-11

Filing date

2010-08-11

Publication date

2020-03-31

2010-08-11 Application filed by Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail filed Critical Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail

2011-02-17 Publication of CA2770275A1 publication Critical patent/CA2770275A1/en

2020-03-31 Application granted granted Critical

2020-03-31 Publication of CA2770275C publication Critical patent/CA2770275C/en

Status Active legal-status Critical Current

2030-08-11 Anticipated expiration legal-status Critical

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Analytical Chemistry (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Immunology (AREA)
Biomedical Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA2770275A 2009-08-11 2010-08-11 An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec Active CA2770275C (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
EP09290621.3		2009-08-11
EP09290621A EP2292799A1 (en)	2009-08-11	2009-08-11	An assay for determining a molecular risk assessment of stec isolates
PCT/IB2010/053631 WO2011018762A1 (en)	2009-08-11	2010-08-11	An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec

Publications (2)

Publication Number	Publication Date
CA2770275A1 CA2770275A1 (en)	2011-02-17
CA2770275C true CA2770275C (en)	2020-03-31

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ID=41479602

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Application Number	Title	Priority Date	Filing Date
CA2770275A Active CA2770275C (en)	2009-08-11	2010-08-11	An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec

Country Status (11)

Country	Link
US (1)	US9416425B2 (es)
EP (2)	EP2292799A1 (es)
JP (1)	JP5990099B2 (es)
KR (1)	KR101837781B1 (es)
AU (1)	AU2010283452B2 (es)
BR (1)	BR112012003122B1 (es)
CA (1)	CA2770275C (es)
ES (1)	ES2614811T3 (es)
MX (1)	MX340945B (es)
NZ (1)	NZ598008A (es)
WO (1)	WO2011018762A1 (es)

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MX340945B (es)	2016-08-01
BR112012003122A2 (pt)	2016-11-22
JP5990099B2 (ja)	2016-09-07
KR101837781B1 (ko)	2018-03-12
CA2770275A1 (en)	2011-02-17
NZ598008A (en)	2014-09-26
EP2464746B1 (en)	2016-11-30
US9416425B2 (en)	2016-08-16
US20120202204A1 (en)	2012-08-09
AU2010283452A1 (en)	2012-03-01
BR112012003122B1 (pt)	2019-10-29
EP2292799A1 (en)	2011-03-09
KR20120100890A (ko)	2012-09-12
EP2464746A1 (en)	2012-06-20
JP2013501516A (ja)	2013-01-17
MX2012001854A (es)	2012-05-22
ES2614811T3 (es)	2017-06-02
AU2010283452B2 (en)	2016-03-17
WO2011018762A1 (en)	2011-02-17

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