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MX2010010310A - Metodo para amplificar un locus en una celula bacteriana. - Google Patents

Metodo para amplificar un locus en una celula bacteriana.

Info

Publication number
MX2010010310A
MX2010010310A MX2010010310A MX2010010310A MX2010010310A MX 2010010310 A MX2010010310 A MX 2010010310A MX 2010010310 A MX2010010310 A MX 2010010310A MX 2010010310 A MX2010010310 A MX 2010010310A MX 2010010310 A MX2010010310 A MX 2010010310A
Authority
MX
Mexico
Prior art keywords
inhibitor
bacterial cell
cells
amplifying
resistant
Prior art date
Application number
MX2010010310A
Other languages
English (en)
Inventor
Eugenio Ferrari
Caroline Peres
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2010010310A publication Critical patent/MX2010010310A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Ciertos aspectos de esta descripción se relacionan a un método para amplificar un locus genómico. En ciertas modalidades, el método puede comprender: a) poner en contacto una población de células hospederas bacterianas con un inhibidor de una enzima esencial, donde las células hospederas bacterianas comprenden un locus genómico de la estructura: A1-P-M-A2, donde A1 y A2 son repeticiones directas, P comprende una secuencia de codificación para un polipéptido, y M comprende una secuencia de codificación para la enzima esencial, y b) seleccionar las células que son resistentes al inhibidor; en donde células que son resistentes al inhibidor tienen copias múltiples de la unidad de amplificación.
MX2010010310A 2008-03-28 2009-03-27 Metodo para amplificar un locus en una celula bacteriana. MX2010010310A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4045608P 2008-03-28 2008-03-28
PCT/US2009/038511 WO2009120929A1 (en) 2008-03-28 2009-03-27 Method for amplifying locus in bacterial cell

Publications (1)

Publication Number Publication Date
MX2010010310A true MX2010010310A (es) 2010-10-08

Family

ID=40801921

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010010310A MX2010010310A (es) 2008-03-28 2009-03-27 Metodo para amplificar un locus en una celula bacteriana.

Country Status (13)

Country Link
US (1) US9528114B2 (es)
EP (1) EP2257629B1 (es)
JP (1) JP5659143B2 (es)
KR (1) KR101683302B1 (es)
CN (1) CN101981193B (es)
AU (1) AU2009228165B2 (es)
BR (1) BRPI0909373A2 (es)
CA (1) CA2719314A1 (es)
DK (1) DK2257629T3 (es)
MX (1) MX2010010310A (es)
NZ (1) NZ587540A (es)
RU (1) RU2577985C2 (es)
WO (1) WO2009120929A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014100920A1 (zh) * 2012-12-28 2014-07-03 安徽华恒生物工程有限公司 生产dl-丙氨酸的工程菌及利用该工程菌生产dl-丙氨酸的方法
CN103540573B (zh) * 2013-10-19 2015-10-07 沅江浣溪沙酶技术有限公司 一种木质素酶及生产方法
KR20230042088A (ko) 2020-07-24 2023-03-27 바스프 에스이 알라닌 라세마아제 단일 결실 및 트랜스 보완

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Also Published As

Publication number Publication date
US20110229938A1 (en) 2011-09-22
JP2011516044A (ja) 2011-05-26
KR101683302B1 (ko) 2016-12-06
AU2009228165A1 (en) 2009-10-01
US9528114B2 (en) 2016-12-27
DK2257629T3 (en) 2016-06-13
EP2257629A1 (en) 2010-12-08
BRPI0909373A2 (pt) 2015-08-04
CN101981193A (zh) 2011-02-23
WO2009120929A8 (en) 2015-10-22
WO2009120929A1 (en) 2009-10-01
JP5659143B2 (ja) 2015-01-28
EP2257629B1 (en) 2016-03-16
CN101981193B (zh) 2016-05-11
KR20100138969A (ko) 2010-12-31
NZ587540A (en) 2012-06-29
AU2009228165B2 (en) 2015-08-13
RU2010144036A (ru) 2012-05-10
RU2577985C2 (ru) 2016-03-20
CA2719314A1 (en) 2009-10-01

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