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MX2010000578A - Metodo para generar aptameros con constantes de disociacion mejoradas. - Google Patents

Metodo para generar aptameros con constantes de disociacion mejoradas.

Info

Publication number
MX2010000578A
MX2010000578A MX2010000578A MX2010000578A MX2010000578A MX 2010000578 A MX2010000578 A MX 2010000578A MX 2010000578 A MX2010000578 A MX 2010000578A MX 2010000578 A MX2010000578 A MX 2010000578A MX 2010000578 A MX2010000578 A MX 2010000578A
Authority
MX
Mexico
Prior art keywords
methods
aptamers
improved
producing
disclosure describes
Prior art date
Application number
MX2010000578A
Other languages
English (en)
Inventor
Larry Gold
Dominic Zichi
Daniel J Schneider
Bruce Eaton
Sheri K Wilcox
Chris Bock
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2010000578(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of MX2010000578A publication Critical patent/MX2010000578A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente descripci?n trata de m?todos SELEX mejorados para producir apt?meros que son capaces de enlazarse a mol?culas objetivo y m?todos photoSELEX mejorados para producir apt?meros fotorreactivos que son capaces de tanto enlazarse como reticularse covalentemente a las mol?culas objetivo. Espec?ficamente, la presente descripci?n trata de m?todos para producir apt?meros y fotoapt?meros que tienen constantes de velocidad de disociaci?n m?s lentas que se obtienen usando los m?todos SELEX y photoSELEX anteriores. La descripci?n adem?s describe apt?meros y fotoapt?meros que tienen constantes de velocidad de disociaci?n m?s lentas que aquellas obtenidas usando los m?todos anteriores. Adem?s, la descripci?n trata de constructos de apt?mero que incluyen una variedad de funcionalidades, que incluyen un elemento escindible, un elemento de detecci?n y un elemento de captura o inmovilizaci?n.
MX2010000578A 2007-07-17 2008-07-17 Metodo para generar aptameros con constantes de disociacion mejoradas. MX2010000578A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US95029307P 2007-07-17 2007-07-17
US95028307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US3142008P 2008-02-26 2008-02-26
US5159408P 2008-05-08 2008-05-08
PCT/US2008/070383 WO2009012418A2 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates

Publications (1)

Publication Number Publication Date
MX2010000578A true MX2010000578A (es) 2010-04-30

Family

ID=40260081

Family Applications (7)

Application Number Title Priority Date Filing Date
MX2010000533A MX2010000533A (es) 2007-07-17 2008-07-17 Analisis multiplexados de muestras de pruebas.
MX2016016043A MX375114B (es) 2007-07-17 2008-07-17 Método para generar aptámeros con constantes de disociación mejoradas.
MX2014005658A MX344253B (es) 2007-07-17 2008-07-17 Metodo para generar aptameros con constantes de disociacion mejoradas.
MX2010000578A MX2010000578A (es) 2007-07-17 2008-07-17 Metodo para generar aptameros con constantes de disociacion mejoradas.
MX2014013401A MX341491B (es) 2007-07-17 2010-01-13 Analisis multiplexados de muestras de pruebas.
MX2016010844A MX355017B (es) 2007-07-17 2010-01-13 Analisis multiplexados de muestras de pruebas.
MX2020009595A MX2020009595A (es) 2007-07-17 2010-01-14 Metodo para generar aptameros con constantes de disociacion mejoradas.

Family Applications Before (3)

Application Number Title Priority Date Filing Date
MX2010000533A MX2010000533A (es) 2007-07-17 2008-07-17 Analisis multiplexados de muestras de pruebas.
MX2016016043A MX375114B (es) 2007-07-17 2008-07-17 Método para generar aptámeros con constantes de disociación mejoradas.
MX2014005658A MX344253B (es) 2007-07-17 2008-07-17 Metodo para generar aptameros con constantes de disociacion mejoradas.

Family Applications After (3)

Application Number Title Priority Date Filing Date
MX2014013401A MX341491B (es) 2007-07-17 2010-01-13 Analisis multiplexados de muestras de pruebas.
MX2016010844A MX355017B (es) 2007-07-17 2010-01-13 Analisis multiplexados de muestras de pruebas.
MX2020009595A MX2020009595A (es) 2007-07-17 2010-01-14 Metodo para generar aptameros con constantes de disociacion mejoradas.

Country Status (17)

Country Link
US (1) US8409795B2 (es)
EP (10) EP2076613B1 (es)
JP (11) JP5404620B2 (es)
KR (3) KR101634608B1 (es)
CN (6) CN101809167B (es)
AU (3) AU2008275917B2 (es)
CA (4) CA3022666C (es)
DK (4) DK2489743T3 (es)
ES (6) ES2533711T3 (es)
FI (1) FI3284832T3 (es)
HR (1) HRP20150382T1 (es)
MX (7) MX2010000533A (es)
NO (1) NO2933340T3 (es)
PL (1) PL2489743T3 (es)
PT (2) PT2489743E (es)
SI (1) SI2489743T1 (es)
WO (3) WO2009012418A2 (es)

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WO2009012410A1 (en) 2009-01-22
AU2008275917A2 (en) 2010-04-29
PL2489743T3 (pl) 2015-06-30
HK1133046A1 (en) 2010-03-12
JP5404620B2 (ja) 2014-02-05
SI2489743T1 (sl) 2015-04-30
EP4056711A1 (en) 2022-09-14
KR20160108584A (ko) 2016-09-19
HK1132012A1 (zh) 2010-02-12
AU2008275917A1 (en) 2009-01-22
PT2489743E (pt) 2015-04-09
JP2016122014A (ja) 2016-07-07
ES2533711T3 (es) 2015-04-14
MX355017B (es) 2018-04-02
HRP20150382T1 (hr) 2015-05-08
DK2489743T3 (en) 2015-03-02
MX375114B (es) 2025-03-06
JP5684568B2 (ja) 2015-03-11
EP2069496A1 (en) 2009-06-17
JP7755619B2 (ja) 2025-10-16
CN107090457A (zh) 2017-08-25
AU2008276001A2 (en) 2010-06-03
AU2008275915A2 (en) 2010-04-22
EP3284832B1 (en) 2023-06-07
MX2010000533A (es) 2010-04-30
CA2696431C (en) 2021-01-05
NO2933340T3 (es) 2018-02-03
KR101656240B1 (ko) 2016-09-09
JP5938080B2 (ja) 2016-06-22
WO2009012418A3 (en) 2009-03-05
ES2647587T3 (es) 2017-12-22
JP2015062416A (ja) 2015-04-09
CA2693453A1 (en) 2009-01-22
CA2693453C (en) 2018-12-11
DK2172566T4 (da) 2022-06-13
AU2008275915A1 (en) 2009-01-22
ES2537322T5 (es) 2022-09-12
EP2626436A3 (en) 2013-10-16
EP2933340A1 (en) 2015-10-21
ES2537322T3 (es) 2015-06-05
ES2604764T3 (es) 2017-03-09
EP2933340B1 (en) 2017-09-06
EP4056711C0 (en) 2025-09-03
JP7303244B2 (ja) 2023-07-04
EP2626436A2 (en) 2013-08-14
MX344253B (es) 2016-12-09
JP5956404B2 (ja) 2016-07-27
EP3284832A2 (en) 2018-02-21
EP2076613A4 (en) 2012-11-28
EP4056711B1 (en) 2025-09-03
KR20100058485A (ko) 2010-06-03
EP2076613B1 (en) 2016-09-07
JP6407195B2 (ja) 2018-10-17
WO2009012418A2 (en) 2009-01-22
JP2010533872A (ja) 2010-10-28
CN101802225B (zh) 2013-10-30
JP6491610B2 (ja) 2019-03-27
AU2008275917B2 (en) 2014-08-21
JP2010533499A (ja) 2010-10-28
EP3284832A3 (en) 2018-03-07
EP2172566B1 (en) 2015-03-04

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