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MX2009008021A - Produccion de una aciltransferasa de lipido de celulas de bacillus licheniformis transformadas. - Google Patents

Produccion de una aciltransferasa de lipido de celulas de bacillus licheniformis transformadas.

Info

Publication number
MX2009008021A
MX2009008021A MX2009008021A MX2009008021A MX2009008021A MX 2009008021 A MX2009008021 A MX 2009008021A MX 2009008021 A MX2009008021 A MX 2009008021A MX 2009008021 A MX2009008021 A MX 2009008021A MX 2009008021 A MX2009008021 A MX 2009008021A
Authority
MX
Mexico
Prior art keywords
lipid acyltransferase
bacillus licheniformis
production
cell
transformed bacillus
Prior art date
Application number
MX2009008021A
Other languages
English (en)
Inventor
Joern Dalgaard Mikkelsen
Rikke Hoeegh Lorentsen
Marc Kolkman
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of MX2009008021A publication Critical patent/MX2009008021A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a un método de producción de una aciltransferasa de lípido que comprende los pasos de: (i) proveer una célula de Bacillus licheniformis; (ii) transformar la célula Bacillus licheniformis con una secuencia de nucleótidos heteróloga que codifica una aciltransferasa de lípido, y (iii) expresar la aciltransferasa de lípido en la célula bajo el control de una secuencia promotora; además, la presente invención se refiere al uso de Bacillus licheniformis para expresar una aciltransferasa de lípido, una célula hospedera de Bacillus licheniformis que comprende una aciltransferasa de lípido heteróloga, y un vector que comprende una secuencia de nucleótidos que codifica una aciltransferasa de lípido enlazada operativamente a una secuencia promotora homóloga a B. licheniformis.
MX2009008021A 2007-01-25 2007-01-25 Produccion de una aciltransferasa de lipido de celulas de bacillus licheniformis transformadas. MX2009008021A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2007/000558 WO2008090395A1 (en) 2007-01-25 2007-01-25 Production of a lipid acyltransferase from transformed bacillus licheniformis cells

Publications (1)

Publication Number Publication Date
MX2009008021A true MX2009008021A (es) 2009-08-07

Family

ID=38323906

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009008021A MX2009008021A (es) 2007-01-25 2007-01-25 Produccion de una aciltransferasa de lipido de celulas de bacillus licheniformis transformadas.

Country Status (11)

Country Link
US (1) US7960150B2 (es)
EP (2) EP2109670A1 (es)
JP (1) JP5124593B2 (es)
CN (1) CN101652474B (es)
AU (1) AU2007344910B2 (es)
BR (1) BRPI0720801A2 (es)
CA (1) CA2673954C (es)
DK (1) DK2405007T5 (es)
MX (1) MX2009008021A (es)
PL (1) PL2405007T3 (es)
WO (1) WO2008090395A1 (es)

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MXPA03010511A (es) 2001-05-18 2004-03-02 Danisco Metodo para preparar una masa con una enzima.
GB0716126D0 (en) * 2007-08-17 2007-09-26 Danisco Process
JP5604032B2 (ja) 2004-07-16 2014-10-08 デュポン ニュートリション バイオサイエンシーズ エーピーエス 食用油の酵素的脱ガム方法
NZ585870A (en) 2007-12-21 2012-04-27 Danisco Process for edible oil refining using a lipid acyltransferase
CN101418276B (zh) * 2008-12-08 2010-12-22 江南大学 一种宿主细胞及其用于重组蛋白高效分泌表达的方法
GB0905367D0 (en) * 2009-03-27 2009-05-13 Danisco Method
CN102656264A (zh) * 2009-10-15 2012-09-05 丹尼斯科公司 脂质酰基转移酶蛋白及其制备方法
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
GB201007668D0 (en) 2010-05-07 2010-06-23 Danisco Method
JP6461604B2 (ja) * 2011-12-27 2019-01-30 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション 脂質製造のための工程
WO2014013538A1 (ja) * 2012-07-17 2014-01-23 国立大学法人福島大学 プラスマローゲンの加水分解方法、プラスマローゲンの測定方法、ホスホリパーゼ含有組成物、ホスホリパーゼの製造方法
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
KR101994252B1 (ko) * 2016-06-30 2019-06-28 명지대학교 산학협력단 베타 아가레이즈의 생산능을 갖는 재조합 바실러스 서브틸리스 균주 및 이의 용도
CN110592100B (zh) * 2019-10-08 2022-08-02 海南大学 一种木薯camta基因及其抑制表达载体的构建和抗病应用
CN119899821B (zh) * 2024-07-01 2025-10-17 华南理工大学 一种紫红链霉菌磷脂酶a2突变体及其应用

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