MX2008008258A - Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo. - Google Patents
Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo.Info
- Publication number
- MX2008008258A MX2008008258A MX2008008258A MX2008008258A MX2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleotide
- modified oligonucleotides
- nucleotide exchange
- targeted nucleotide
- dna sequence
- Prior art date
Links
- 125000003729 nucleotide group Chemical group 0.000 title abstract 6
- 239000002773 nucleotide Substances 0.000 title abstract 5
- -1 propynyl modified oligonucleotides Chemical class 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C12N15/09—Recombinant DNA-technology
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- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
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- Health & Medical Sciences (AREA)
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- Zoology (AREA)
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- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Un método y oligonucleótidos para intercambio de nucleótido dirigido de una secuencia de ADN dúplex, en donde el oligonucleótido donador contiene por lo menos un nucleótido modificado que es una purina y/o pirimidina propinilada que tiene una afinidad de unión superior comparada con A, C, T o G que ocurren naturalmente y/o se une fuertemente a un nucleótido en una posición opuesta en la primera secuencia de ADN en comparación con un nucleótido que ocurre naturalmente complementario al nucleótido en la posición opuesta en la primera secuencia de ADN.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/NL2005/000884 WO2007073149A1 (en) | 2005-12-22 | 2005-12-22 | Alternative nucleotides for improved targeted nucleotide exchange |
| PCT/NL2006/000244 WO2007073154A1 (en) | 2005-12-22 | 2006-05-09 | Alternative nucleotides for improved targeted nucleotide exchange |
| PCT/NL2006/000649 WO2007073166A1 (en) | 2005-12-22 | 2006-12-20 | Improved targeted nucleotide exchange with propynyl modified oligonucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2008008258A true MX2008008258A (es) | 2008-09-24 |
Family
ID=36691461
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2008008258A MX2008008258A (es) | 2005-12-22 | 2006-12-20 | Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo. |
| MX2008008261A MX2008008261A (es) | 2005-12-22 | 2006-12-21 | Intercambio nucleotidico dirigido mejorado con oligonucleotidos modificados por lna. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2008008261A MX2008008261A (es) | 2005-12-22 | 2006-12-21 | Intercambio nucleotidico dirigido mejorado con oligonucleotidos modificados por lna. |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US20090307805A1 (es) |
| EP (4) | EP2333115A1 (es) |
| JP (3) | JP5295780B2 (es) |
| KR (2) | KR20080092924A (es) |
| CN (3) | CN101346474B (es) |
| AU (2) | AU2006328050B2 (es) |
| BR (2) | BRPI0621134A2 (es) |
| CA (2) | CA2633741A1 (es) |
| ES (2) | ES2393277T3 (es) |
| IL (2) | IL192164A0 (es) |
| MX (2) | MX2008008258A (es) |
| NZ (2) | NZ569503A (es) |
| RU (2) | RU2008130093A (es) |
| WO (4) | WO2007073149A1 (es) |
| ZA (2) | ZA200805091B (es) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007073149A1 (en) * | 2005-12-22 | 2007-06-28 | Keygene N.V. | Alternative nucleotides for improved targeted nucleotide exchange |
| KR20100051795A (ko) * | 2007-06-22 | 2010-05-18 | 키진 엔.브이. | 향상된 변형 올리고뉴클레오티드를 이용한 표적화 뉴클레오티드 교환 |
| RU2515110C2 (ru) * | 2007-12-21 | 2014-05-10 | Киджин Н.В. | Улучшенный способ мутагенеза с использованием полиэтиленгликоль-опосредованного введения мутагенных нуклеиновых оснований в растительные протопласты |
| CN103146757B (zh) * | 2007-12-21 | 2016-05-04 | 凯津公司 | 通过聚乙二醇的介导将致诱变核碱基引入植物原生质体的改进的诱变方法 |
| JP2012513199A (ja) * | 2008-12-22 | 2012-06-14 | キージーン・エン・フェー | 植物プロトプラストにおける標的化遺伝子改変の効率を増大させるための二本鎖ｒｎａの使用 |
| CA2951341A1 (en) | 2009-12-21 | 2011-06-30 | Keygene N.V. | Improved techniques for transfecting protoplasts |
| AU2011337323B2 (en) * | 2010-12-02 | 2015-11-26 | Keygene N.V. | Targeted alteration of DNA |
| ES2536640T3 (es) | 2010-12-02 | 2015-05-27 | Keygene N.V. | Alteración dirigida de ADN con oligonucleótidos |
| US20140206850A1 (en) | 2011-04-29 | 2014-07-24 | Keygene N.V. | Glyphosate resistance enhancement |
| WO2012174187A2 (en) * | 2011-06-15 | 2012-12-20 | Grifols Therapeutics Inc. | Methods, compositions, and kits for determining human immunodeficiency virus (hiv) |
| EP2554045A1 (en) | 2011-08-04 | 2013-02-06 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Method for systemically influencing processes in the male meiocyte |
| EP2581448B1 (en) * | 2011-10-13 | 2015-01-28 | Association Institut de Myologie | Tricyclo-phosphorothioate DNA |
| CN102827251B (zh) * | 2012-09-12 | 2014-06-18 | 中国人民解放军第四军医大学 | 一种透膜肽介导的反义抗菌剂及其制备方法和应用 |
| EA032843B9 (ru) | 2012-09-28 | 2019-11-29 | Nunhems Bv | Растения solanum lycopersicum, имеющие нетрансгенные модификации в гене acs4 |
| EA032773B1 (ru) | 2012-11-21 | 2019-07-31 | Нунхемс Б.В. | Растения solanum lycopersicum, имеющие нетрансгенные изменения в гене acs2 |
| AU2014211518B2 (en) | 2013-01-31 | 2019-08-01 | Nunhems B.V. | Solanum lycopersicum plants having pink fruits |
| US12331303B2 (en) | 2013-03-15 | 2025-06-17 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| PL3527068T3 (pl) | 2013-03-15 | 2022-10-10 | Cibus Us Llc | Sposoby i kompozycje do zwiększania skuteczności ukierunkowanej modyfikacji genu z użyciem naprawy genu, w której pośredniczy oligonukleotyd |
| US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
| JP2013247961A (ja) * | 2013-08-21 | 2013-12-12 | Keygene Nv | 植物細胞プロトプラストにおける二本鎖アクセプタｄｎａ配列の標的化改変の方法 |
| EA201691581A1 (ru) | 2014-03-14 | 2017-02-28 | Кибус Юс Ллс | Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов |
| EP4372091A3 (en) * | 2014-12-12 | 2024-07-31 | Tod M. Woolf | Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides |
| WO2016105185A1 (en) | 2014-12-22 | 2016-06-30 | Keygene N.V. | Plant callus populations |
| AU2016264193B2 (en) | 2015-05-19 | 2022-04-07 | The National Centre For Biological Sciences | Methods and composition for determining pH |
| EP3402745B1 (en) * | 2015-12-23 | 2023-07-26 | The Regents of the University of California | Nano-sensors for nucleic acid detection and discrimination |
| KR20250117482A (ko) | 2016-02-09 | 2025-08-04 | 시버스 유에스 엘엘씨 | 올리고뉴클레오타이드 매개된 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물 |
| EP3257944A1 (en) | 2016-06-14 | 2017-12-20 | Nunhems B.V. | Tomato plants having alterations in the dmr6 gene |
| WO2018115389A1 (en) | 2016-12-22 | 2018-06-28 | Keygene N.V. | Methods of targeted genetic alteration in plant cells |
| AU2019207703B2 (en) | 2018-01-09 | 2024-11-14 | Cibus Europe B.V. | Shatterproof genes and mutations |
| CA3095047A1 (en) | 2018-04-04 | 2019-10-10 | Cibus Us Llc | Fad2 genes and mutations |
| NL2020823B1 (en) | 2018-04-25 | 2019-11-05 | Univ Delft Tech | NAD+ dependent 7ß-hydroxysteroid dehydrogenase |
| EP3363751B1 (de) | 2018-06-05 | 2020-04-22 | B&R Industrial Automation GmbH | Verfahren zur übergabe einer transporteinheit eines langstatorlinearmotors an einer übergabeposition |
| WO2020011985A1 (en) | 2018-07-12 | 2020-01-16 | Keygene N.V. | Type v crispr/nuclease-system for genome editing in plant cells |
| US20220010321A1 (en) | 2018-11-01 | 2022-01-13 | Keygene N.V. | Dual guide rna for crispr/cas genome editing in plants cells |
| CN114262735B (zh) * | 2021-12-15 | 2025-09-16 | 成都齐碳科技有限公司 | 用于表征多核苷酸的衔接体及其用途 |
| WO2023168273A2 (en) * | 2022-03-01 | 2023-09-07 | Colorado State University Research Foundation | Targeted control of weeds and invasive plants by delivery of fana antisense oligonucleotides |
| US20250215424A1 (en) * | 2022-03-30 | 2025-07-03 | National Institute Of Advanced Industrial Science And Technology | Single-strand form polynucleotide and use thereof in genome editing |
| JP7660960B2 (ja) * | 2023-04-26 | 2025-04-14 | Ｅｕｒｕｓ Ｔｈｅｒａｐｅｕｔｉｃｓ株式会社 | 標的ヌクレオチド配列の改変のための非天然型ポリヌクレオチド |
| WO2025110249A1 (ja) * | 2023-11-24 | 2025-05-30 | 国立大学法人 広島大学 | 塩基配列編集用組成物およびそれを使用する塩基配列編集方法 |
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| AU2002341905A2 (en) | 2001-09-27 | 2003-04-07 | University Of Delaware | Composition and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration |
| DK2264172T3 (da) * | 2002-04-05 | 2017-11-27 | Roche Innovation Ct Copenhagen As | Oligomerforbindelser til modulering af HIF-1á-ekspression |
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| DK1633767T3 (en) * | 2003-06-02 | 2019-03-25 | Univ Massachusetts | METHODS AND COMPOSITIONS FOR MANAGING THE EFFECT OF RNA SILENCING |
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| WO2007073149A1 (en) * | 2005-12-22 | 2007-06-28 | Keygene N.V. | Alternative nucleotides for improved targeted nucleotide exchange |
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